Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004532

Physical Location & Seq

Genomic Coordinates Forward (+)

40448008 .. 40448776

Gene Structure 769 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1269200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 654 bp

ATGGCAAACCAAATCCTGCTATTGACTACTTTCCTAGCCATGACATGTTCTCTGGTCGTTGCTTTCGAGCCTAGTCCGTTGCAGGATTTCTGTGTTGCTGATGCAACTAGCTCAGGAACAAGAGTGAATGGGCTACCTTGCTTGGACTCCAAGCTAGCAACAGCTGAGCATTTCTCCTTCAGTGGACTTCATATCCCAGGCAACACCTCAAACCCTGTTGGTGGAAAAGTTACCCCCGTCAATGTGGCTCAAATTCCAGGACTCAACACCCTTGGCATCTCACTCGCTCGCATTGACTACGCACCAATGGGCGTCATCCCACCCCACACTCATCCCCGTGCCACTGAGATTTTAACCGTCTTAGAAGGCAGCCTCGATGTTGGCTTCGTGACCTCAAACCCCGACAACAAGCTCATCTCAAAGGTCCTTAACAAGGGTGATGCGTTTGTGTTCCCTGTCGGACTAGTTCACTATCAGAAAAATGTGGGCACTGGAATGGCCATTTCACTCTCTTCTTTGAGCAGCCAAAACCCTGGAGTCAACACCATTGCAAATGCTGTGTTTGGATCCAATCCCGCAATTCCTGAGGATGTTTTGGCCAAGTCTTTCCAGGTGGACAAATCTGTAATTCGTGATCTTCAAGCAAAATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006810 GO:0008150 GO:0009506 GO:0009987 GO:0010496 GO:0010497 GO:0030054 GO:0030312 GO:0044464 GO:0050789 GO:0050793 GO:0051179 GO:0051234 GO:0051239 GO:0055044 GO:0065007 GO:0071944 GO:2000026 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_1

Protein Analysis

218

Amino Acids

22.75

Weight (kDa)

6.17

Isoelectric Point (pI)

29.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cupin_1	PF00190	62 - 209	3.3e-51	Cupin
Cupin_2	PF07883	98 - 167	1.6e-09	Cupin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004532)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g10240 FvH4_3g10730
malus_domestica	MD05G1269200.v1.1 MD05G1271400.v1.1 MD05G1271500.v1.1 MD10G1248000.v1.1 MD10G1251900.v1.1 MD10G1252000.v1.1 MD14G1081700.v1.1
prunus_persica	Prupe.4G091500_v2.0.a1 Prupe.4G095500_v2.0.a1 Prupe.4G095600_v2.0.a1
pyrus_communis	pycom10g20550 pycom10g20790
rosa_chinensis	RchiOBHm_Chr5g0016481 RchiOBHm_Chr5g0017421
rosa_laevigata	RLG00000032286
rosa_multiflora	Rmu_co8334417.1_g000001 Rmu_sc0001235.1_g000024 Rmu_sc0007392.1_g000005 Rmu_sc0019163.1_g000002
rosa_roxburghii	Rroxscaffold_1G00059970
rosa_rugosa	Rorug05G0032500 Rorug05G0035300
rosa_samantha	Rh5AG126500 Rh5AG130000 Rh5BG125300 Rh5CG137900 Rh5DG125500 Rh5DG130300
rosa_wichuraiana	Rw5G010960 Rw5G011230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	576
AclWI	GGATC	2 cut(s)	561, 574
AcoI	YGGCCR	2 cut(s)	498, 597
AcsI	RAATTY	2 cut(s)	252, 647
AcuI	CTGAAG	1 cut(s)	163
AcyI	GRCGYC	1 cut(s)	312
AfiI	CCNNNNNNNGG	2 cut(s)	221, 433
AflIII	ACRYGT	1 cut(s)	44
AgsI	TTSAA	1 cut(s)	641
AhlI	ACTAGT	1 cut(s)	463
AjnI	CCWGG	4 cut(s)	196, 256, 532, 609
AluBI	AGCT	4 cut(s)	111, 154, 164, 412
AluI	AGCT	4 cut(s)	111, 154, 164, 412
AlwI	GGATC	2 cut(s)	561, 574
AoxI	GGCC	2 cut(s)	498, 597
ApeKI	GCWGC	2 cut(s)	369, 522
ApoI	RAATTY	2 cut(s)	252, 647
AspS9I	GGNCC	1 cut(s)	424
AsuHPI	GGTGA	1 cut(s)	449
AsuNHI	GCTAGC	1 cut(s)	154
AvaII	GGWCC	1 cut(s)	424
AxyI	CCTNAGG	1 cut(s)	585
BaeGI	GKGCMC	1 cut(s)	491
BalI	TGGCCA	2 cut(s)	500, 599
BamHI	GGATCC	1 cut(s)	566
BbvI	GCAGC	2 cut(s)	381, 534
BcgI	CGANNNNNNTGC	2 cut(s)	265, 299
BciT130I	CCWGG	4 cut(s)	198, 258, 534, 611
BcuI	ACTAGT	1 cut(s)	463
BfaI	CTAG	5 cut(s)	35, 72, 108, 155, 464
BisI	GCNGC	2 cut(s)	370, 523
BlpI	GCTNAGC	1 cut(s)	165
BlsI	GCNGC	2 cut(s)	371, 524
Bme1390I	CCNGG	4 cut(s)	198, 258, 534, 611
Bme18I	GGWCC	1 cut(s)	424
BmgT120I	GGNCC	1 cut(s)	424
BmiI	GGNNCC	1 cut(s)	568
BmrFI	CCNGG	4 cut(s)	198, 258, 534, 611
BmsI	GCATC	3 cut(s)	91, 285, 430
BmtI	GCTAGC	1 cut(s)	158
BplI	GAGNNNNNCTC	2 cut(s)	158, 190
BpmI	CTGGAG	1 cut(s)	555
Bpu10I	CCTNAGC	1 cut(s)	112
Bpu1102I	GCTNAGC	1 cut(s)	165
BsaHI	GRCGYC	1 cut(s)	312
BsaJI	CCNNGG	3 cut(s)	196, 271, 532
Bsc4I	CCNNNNNNNGG	2 cut(s)	221, 433
Bse1I	ACTGG	1 cut(s)	496
Bse21I	CCTNAGG	1 cut(s)	585
Bse3DI	GCAATG	1 cut(s)	546
BseBI	CCWGG	4 cut(s)	198, 258, 534, 611
BseDI	CCNNGG	3 cut(s)	196, 271, 532
BseGI	GGATG	3 cut(s)	315, 331, 595
BseLI	CCNNNNNNNGG	2 cut(s)	221, 433
BseMI	GCAATG	1 cut(s)	546
BseMII	CTCAG	4 cut(s)	126, 156, 336, 576
BseNI	ACTGG	1 cut(s)	496
BseSI	GKGCMC	1 cut(s)	491
BseXI	GCAGC	2 cut(s)	381, 534
BshFI	GGCC	2 cut(s)	500, 599
BslI	CCNNNNNNNGG	2 cut(s)	221, 433
BsnI	GGCC	2 cut(s)	500, 599
Bsp1286I	GDGCHC	1 cut(s)	491
Bsp143I	GATC	2 cut(s)	566, 634
Bsp1720I	GCTNAGC	1 cut(s)	165
BspACI	CCGC	1 cut(s)	576
BspANI	GGCC	2 cut(s)	500, 599
BspCNI	CTCAG	4 cut(s)	125, 157, 337, 577
BspLI	GGNNCC	1 cut(s)	568
BspOI	GCTAGC	1 cut(s)	158
BspPI	GGATC	2 cut(s)	561, 574
BsrDI	GCAATG	1 cut(s)	546
BsrI	ACTGG	1 cut(s)	496
BssECI	CCNNGG	3 cut(s)	196, 271, 532
BssMI	GATC	2 cut(s)	566, 634
BssNI	GRCGYC	1 cut(s)	312
BssT1I	CCWWGG	1 cut(s)	271
Bst2UI	CCWGG	4 cut(s)	198, 258, 534, 611
Bst4CI	ACNGT	1 cut(s)	358
Bst6I	CTCTTC	1 cut(s)	517
BstACI	GRCGYC	1 cut(s)	312
BstC8I	GCNNGC	2 cut(s)	156, 289
BstDEI	CTNAG	5 cut(s)	112, 165, 345, 361, 585
BstENI	CCTNNNNNAGG	1 cut(s)	431
BstF5I	GGATG	3 cut(s)	315, 331, 595
BstKTI	GATC	2 cut(s)	569, 637
BstMBI	GATC	2 cut(s)	566, 634
BstNI	CCWGG	4 cut(s)	198, 258, 534, 611
BstNSI	RCATGY	1 cut(s)	48
BstSCI	CCNGG	4 cut(s)	196, 256, 532, 609
BstSLI	GKGCMC	1 cut(s)	491
BstV1I	GCAGC	2 cut(s)	381, 534
BstX2I	RGATCY	1 cut(s)	566
BstXI	CCANNNNNNTGG	1 cut(s)	533
BstYI	RGATCY	1 cut(s)	566
Bsu36I	CCTNAGG	1 cut(s)	585
BsuRI	GGCC	2 cut(s)	500, 599
BtsCI	GGATG	3 cut(s)	315, 331, 595
BtsIMutI	CAGTG	3 cut(s)	187, 342, 489
Cac8I	GCNNGC	2 cut(s)	156, 289
Cfr13I	GGNCC	1 cut(s)	424
CseI	GACGC	1 cut(s)	301
CviAII	CATG	2 cut(s)	40, 45
DdeI	CTNAG	5 cut(s)	112, 165, 345, 361, 585
DpnI	GATC	2 cut(s)	568, 636
DpnII	GATC	2 cut(s)	566, 634
EaeI	YGGCCR	2 cut(s)	498, 597
Eam1104I	CTCTTC	1 cut(s)	517
EarI	CTCTTC	1 cut(s)	517
Eco130I	CCWWGG	1 cut(s)	271
Eco47I	GGWCC	1 cut(s)	424
Eco57I	CTGAAG	1 cut(s)	163
Eco81I	CCTNAGG	1 cut(s)	585
EcoNI	CCTNNNNNAGG	1 cut(s)	431
EcoO109I	RGGNCCY	1 cut(s)	424
EcoRII	CCWGG	4 cut(s)	196, 256, 532, 609
EcoT14I	CCWWGG	1 cut(s)	271
ErhI	CCWWGG	1 cut(s)	271
FaeI	CATG	2 cut(s)	43, 48
FaiI	YATR	3 cut(s)	41, 46, 192
FatI	CATG	2 cut(s)	39, 44
FauI	CCCGC	1 cut(s)	583
Fnu4HI	GCNGC	2 cut(s)	370, 523
FokI	GGATG	3 cut(s)	302, 318, 602
Fsp4HI	GCNGC	2 cut(s)	370, 523
FspBI	CTAG	5 cut(s)	35, 72, 108, 155, 464
GluI	GCNGC	2 cut(s)	370, 523
GsuI	CTGGAG	1 cut(s)	555
HaeIII	GGCC	2 cut(s)	500, 599
HgaI	GACGC	1 cut(s)	301
Hin1I	GRCGYC	1 cut(s)	312
Hin1II	CATG	2 cut(s)	43, 48
HincII	GTYRAC	1 cut(s)	541
HindII	GTYRAC	1 cut(s)	541
HinfI	GANTC	3 cut(s)	146, 261, 537
HphI	GGTGA	1 cut(s)	449
Hpy166II	GTNNAC	4 cut(s)	185, 469, 541, 616
Hpy188I	TCNGA	2 cut(s)	461, 477
Hpy188III	TCNNGA	4 cut(s)	114, 388, 584, 632
Hpy8I	GTNNAC	4 cut(s)	185, 469, 541, 616
HpyAV	CCTTC	2 cut(s)	187, 359
HpyCH4III	ACNGT	1 cut(s)	358
HpyCH4V	TGCA	3 cut(s)	82, 104, 551
HpyF3I	CTNAG	5 cut(s)	112, 165, 345, 361, 585
Hsp92I	GRCGYC	1 cut(s)	312
Hsp92II	CATG	2 cut(s)	43, 48
Kzo9I	GATC	2 cut(s)	566, 634
Lsp1109I	GCAGC	2 cut(s)	381, 534
LweI	GCATC	3 cut(s)	91, 285, 430
MaeI	CTAG	5 cut(s)	35, 72, 108, 155, 464
MaeIII	GTNAC	2 cut(s)	229, 388
MalI	GATC	2 cut(s)	568, 636
MboI	GATC	2 cut(s)	566, 634
MboII	GAAGA	2 cut(s)	504, 629
MflI	RGATCY	1 cut(s)	566
MhlI	GDGCHC	1 cut(s)	491
MlsI	TGGCCA	2 cut(s)	500, 599
MluCI	AATT	4 cut(s)	252, 579, 627, 647
MluNI	TGGCCA	2 cut(s)	500, 599
MlyI	GAGTC	3 cut(s)	140, 255, 546
MmeI	TCCRAC	1 cut(s)	439
MnlI	CCTC	4 cut(s)	217, 383, 403, 580
Mox20I	TGGCCA	2 cut(s)	500, 599
MscI	TGGCCA	2 cut(s)	500, 599
MseI	TTAA	2 cut(s)	353, 429
MslI	CAYNNNNRTG	2 cut(s)	336, 494
Msp20I	TGGCCA	2 cut(s)	500, 599
MspA1I	CMGCKG	1 cut(s)	164
MspR9I	CCNGG	4 cut(s)	198, 258, 534, 611
MvaI	CCWGG	4 cut(s)	198, 258, 534, 611
NdeII	GATC	2 cut(s)	566, 634
NheI	GCTAGC	1 cut(s)	154
NlaIII	CATG	2 cut(s)	43, 48
NlaIV	GGNNCC	1 cut(s)	568
NmuCI	GTSAC	1 cut(s)	388
NspI	RCATGY	1 cut(s)	48
PciI	ACATGT	1 cut(s)	44
PcsI	WCGNNNNNNNCGW	1 cut(s)	63
PfoI	TCCNGGA	1 cut(s)	256
PkrI	GCNGC	2 cut(s)	371, 524
PleI	GAGTC	3 cut(s)	140, 255, 545
PpsI	GAGTC	3 cut(s)	140, 255, 545
PpuMI	RGGWCCY	1 cut(s)	424
PscI	ACATGT	1 cut(s)	44
Psp5II	RGGWCCY	1 cut(s)	424
Psp6I	CCWGG	4 cut(s)	196, 256, 532, 609
PspGI	CCWGG	4 cut(s)	196, 256, 532, 609
PspN4I	GGNNCC	1 cut(s)	568
PspPI	GGNCC	1 cut(s)	424
PspPPI	RGGWCCY	1 cut(s)	424
PsuI	RGATCY	1 cut(s)	566
PvuII	CAGCTG	1 cut(s)	164
RseI	CAYNNNNRTG	2 cut(s)	336, 494
SaqAI	TTAA	2 cut(s)	353, 429
SatI	GCNGC	2 cut(s)	370, 523
Sau3AI	GATC	2 cut(s)	566, 634
Sau96I	GGNCC	1 cut(s)	424
SchI	GAGTC	3 cut(s)	140, 255, 546
ScrFI	CCNGG	4 cut(s)	198, 258, 534, 611
SduI	GDGCHC	1 cut(s)	491
SetI	ASST	9 cut(s)	113, 139, 156, 166, 209, 395, 414, 426, 615
SfaNI	GCATC	3 cut(s)	91, 285, 430
SinI	GGWCC	1 cut(s)	424
SmiMI	CAYNNNNRTG	2 cut(s)	336, 494
SpeI	ACTAGT	1 cut(s)	463
Sse9I	AATT	4 cut(s)	252, 579, 627, 647
SsiI	CCGC	1 cut(s)	576
SspMI	CTAG	5 cut(s)	35, 72, 108, 155, 464
StyD4I	CCNGG	4 cut(s)	196, 256, 532, 609
StyI	CCWWGG	1 cut(s)	271
TaaI	ACNGT	1 cut(s)	358
TaqI	TCGA	2 cut(s)	66, 375
TasI	AATT	4 cut(s)	252, 579, 627, 647
Tru1I	TTAA	2 cut(s)	353, 429
Tru9I	TTAA	2 cut(s)	353, 429
TscAI	CASTG	3 cut(s)	187, 349, 496
TseFI	GTSAC	1 cut(s)	388
TseI	GCWGC	2 cut(s)	369, 522
Tsp45I	GTSAC	1 cut(s)	388
TspDTI	ATGAA	1 cut(s)	179
TspGWI	ACGGA	1 cut(s)	66
TspRI	CASTG	3 cut(s)	187, 349, 496
VpaK11BI	GGWCC	1 cut(s)	424
XagI	CCTNNNNNAGG	1 cut(s)	431
XapI	RAATTY	2 cut(s)	252, 647
XceI	RCATGY	1 cut(s)	48
XspI	CTAG	5 cut(s)	35, 72, 108, 155, 464

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.