Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004532

Physical Location & Seq

Genomic Coordinates Reverse (-)

40689457 .. 40690014

Gene Structure 558 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1271400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 558 bp

ATGTGTGCACATATCACAGCAACGATAGTAAATGGGTTTCCTTGCTTGGACTCCAAGCTAGCAACAGCTGAGCATTTCTTCTTCAGCGGACTTCACATCCCCGGCAACACCTCAAACCCTGTTGGTGGAAAAGTCACCCCTGTCAATGTGGGTCAAATTCCAGGTCTCAACACCCTCGGCATCTCACTCGCTCGCATCGACTACGCACCATGGGGTGTTATCCCACCCCACACTCATCCCCGTGCCACTGAGATTTTAACCGTCTTAGAAGGCAGCCTCAATGTTGGCTTTGTGACATCAAACCCCAACAACAAGCTCATTTCAAAGGTCCTTAACAAGGGTGATGTGTTTGTGTTCCCTGTTGGACTAGTTCACTATCAGCAAAATGTGGGGAATGGAATTGCCATTTCACTCTCTTCTCTGAGTAGCCAAAACCCGGGAGTCAACACCATTGCTAATGCTGTGTTTGGATCCAATCCCGCAATTTCAGAAGATGTTCTGGCTAAATCTTTCCAGGTTGACAAATCAGTAATTAGTAGCATTCAAGCTAAATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006810 GO:0008150 GO:0009506 GO:0009987 GO:0010496 GO:0010497 GO:0030054 GO:0030312 GO:0044464 GO:0050789 GO:0050793 GO:0051179 GO:0051234 GO:0051239 GO:0055044 GO:0065007 GO:0071944 GO:2000026 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_1

Protein Analysis

186

Amino Acids

19.4

Weight (kDa)

7.85

Isoelectric Point (pI)

24.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cupin_1	PF00190	30 - 177	1.5e-50	Cupin
Cupin_2	PF07883	66 - 135	8e-09	Cupin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004532)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g10240 FvH4_3g10730
malus_domestica	MD05G1269200.v1.1 MD05G1271400.v1.1 MD05G1271500.v1.1 MD10G1248000.v1.1 MD10G1251900.v1.1 MD10G1252000.v1.1 MD14G1081700.v1.1
prunus_persica	Prupe.4G091500_v2.0.a1 Prupe.4G095500_v2.0.a1 Prupe.4G095600_v2.0.a1
pyrus_communis	pycom10g20550 pycom10g20790
rosa_chinensis	RchiOBHm_Chr5g0016481 RchiOBHm_Chr5g0017421
rosa_laevigata	RLG00000032286
rosa_multiflora	Rmu_co8334417.1_g000001 Rmu_sc0001235.1_g000024 Rmu_sc0007392.1_g000005 Rmu_sc0019163.1_g000002
rosa_roxburghii	Rroxscaffold_1G00059970
rosa_rugosa	Rorug05G0032500 Rorug05G0035300
rosa_samantha	Rh5AG126500 Rh5AG130000 Rh5BG125300 Rh5CG137900 Rh5DG125500 Rh5DG130300
rosa_wichuraiana	Rw5G010960 Rw5G011230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	87, 480
AclWI	GGATC	2 cut(s)	465, 478
AcsI	RAATTY	2 cut(s)	156, 551
AcuI	CTGAAG	1 cut(s)	67
AfiI	CCNNNNNNNGG	3 cut(s)	125, 337, 436
AgsI	TTSAA	2 cut(s)	324, 545
AhlI	ACTAGT	1 cut(s)	367
AjnI	CCWGG	2 cut(s)	160, 513
AluBI	AGCT	4 cut(s)	58, 68, 316, 548
AluI	AGCT	4 cut(s)	58, 68, 316, 548
Alw21I	GWGCWC	1 cut(s)	10
Alw26I	GTCTC	1 cut(s)	170
Alw44I	GTGCAC	1 cut(s)	6
AlwI	GGATC	2 cut(s)	465, 478
Ama87I	CYCGRG	1 cut(s)	436
ApaLI	GTGCAC	1 cut(s)	6
ApeKI	GCWGC	1 cut(s)	273
ApoI	RAATTY	2 cut(s)	156, 551
ArsI	GACNNNNNNTTYG	2 cut(s)	148, 180
Asp700I	GAANNNNTTC	1 cut(s)	495
AspS9I	GGNCC	1 cut(s)	328
AsuC2I	CCSGG	3 cut(s)	102, 437, 438
AsuHPI	GGTGA	2 cut(s)	127, 353
AsuNHI	GCTAGC	1 cut(s)	58
AvaI	CYCGRG	1 cut(s)	436
AvaII	GGWCC	1 cut(s)	328
BaeGI	GKGCMC	1 cut(s)	10
BamHI	GGATCC	1 cut(s)	470
Bbv12I	GWGCWC	1 cut(s)	10
BbvI	GCAGC	1 cut(s)	285
BcgI	CGANNNNNNTGC	2 cut(s)	169, 203
BciT130I	CCWGG	2 cut(s)	162, 515
BcnI	CCSGG	3 cut(s)	102, 437, 438
BcoDI	GTCTC	1 cut(s)	170
BcuI	ACTAGT	1 cut(s)	367
BfaI	CTAG	2 cut(s)	59, 368
BisI	GCNGC	1 cut(s)	274
BlpI	GCTNAGC	1 cut(s)	69
BlsI	GCNGC	1 cut(s)	275
Bme1390I	CCNGG	5 cut(s)	102, 162, 437, 438, 515
Bme18I	GGWCC	1 cut(s)	328
BmeT110I	CYCGRG	1 cut(s)	436
BmgT120I	GGNCC	1 cut(s)	328
BmiI	GGNNCC	1 cut(s)	472
BmrFI	CCNGG	5 cut(s)	102, 162, 437, 438, 515
BmsI	GCATC	2 cut(s)	189, 204
BmtI	GCTAGC	1 cut(s)	62
Bpu1102I	GCTNAGC	1 cut(s)	69
BpuMI	CCSGG	3 cut(s)	102, 437, 438
BsaI	GGTCTC	1 cut(s)	170
BsaJI	CCNNGG	4 cut(s)	100, 175, 209, 436
Bsc4I	CCNNNNNNNGG	3 cut(s)	125, 337, 436
Bse3DI	GCAATG	1 cut(s)	450
BseBI	CCWGG	2 cut(s)	162, 515
BseDI	CCNNGG	4 cut(s)	100, 175, 209, 436
BseGI	GGATG	2 cut(s)	96, 235
BseLI	CCNNNNNNNGG	3 cut(s)	125, 337, 436
BseMI	GCAATG	1 cut(s)	450
BseMII	CTCAG	3 cut(s)	60, 240, 413
BseSI	GKGCMC	1 cut(s)	10
BseXI	GCAGC	1 cut(s)	285
BsiHKAI	GWGCWC	1 cut(s)	10
BsiHKCI	CYCGRG	1 cut(s)	436
BsiSI	CCGG	2 cut(s)	102, 437
BslI	CCNNNNNNNGG	3 cut(s)	125, 337, 436
BsmAI	GTCTC	1 cut(s)	170
BsmI	GAATGC	1 cut(s)	540
Bso31I	GGTCTC	1 cut(s)	170
BsoBI	CYCGRG	1 cut(s)	436
Bsp1286I	GDGCHC	1 cut(s)	10
Bsp143I	GATC	1 cut(s)	470
Bsp1720I	GCTNAGC	1 cut(s)	69
Bsp19I	CCATGG	1 cut(s)	209
BspACI	CCGC	2 cut(s)	87, 480
BspCNI	CTCAG	3 cut(s)	61, 241, 414
BspLI	GGNNCC	1 cut(s)	472
BspOI	GCTAGC	1 cut(s)	62
BspPI	GGATC	2 cut(s)	465, 478
BspTNI	GGTCTC	1 cut(s)	170
BsrDI	GCAATG	1 cut(s)	450
BssECI	CCNNGG	4 cut(s)	100, 175, 209, 436
BssMI	GATC	1 cut(s)	470
BssT1I	CCWWGG	1 cut(s)	209
Bst2UI	CCWGG	2 cut(s)	162, 515
Bst4CI	ACNGT	1 cut(s)	262
Bst6I	CTCTTC	1 cut(s)	421
BstC8I	GCNNGC	2 cut(s)	60, 193
BstDEI	CTNAG	4 cut(s)	69, 249, 265, 422
BstDSI	CCRYGG	1 cut(s)	209
BstENI	CCTNNNNNAGG	1 cut(s)	335
BstF5I	GGATG	2 cut(s)	96, 235
BstKTI	GATC	1 cut(s)	473
BstMAI	GTCTC	1 cut(s)	170
BstMBI	GATC	1 cut(s)	470
BstNI	CCWGG	2 cut(s)	162, 515
BstSCI	CCNGG	5 cut(s)	100, 160, 435, 436, 513
BstSLI	GKGCMC	1 cut(s)	10
BstV1I	GCAGC	1 cut(s)	285
BstX2I	RGATCY	1 cut(s)	470
BstYI	RGATCY	1 cut(s)	470
BtgI	CCRYGG	1 cut(s)	209
BtsCI	GGATG	2 cut(s)	96, 235
BtsIMutI	CAGTG	1 cut(s)	246
Cac8I	GCNNGC	2 cut(s)	60, 193
Cfr13I	GGNCC	1 cut(s)	328
Cfr9I	CCCGGG	1 cut(s)	436
CviAII	CATG	1 cut(s)	210
CviJI	RGCY	8 cut(s)	58, 68, 276, 288, 316, 429, 503, 548
CviKI_1	RGCY	8 cut(s)	58, 68, 276, 288, 316, 429, 503, 548
DdeI	CTNAG	4 cut(s)	69, 249, 265, 422
DpnI	GATC	1 cut(s)	472
DpnII	GATC	1 cut(s)	470
Eam1104I	CTCTTC	1 cut(s)	421
EarI	CTCTTC	1 cut(s)	421
Eco130I	CCWWGG	1 cut(s)	209
Eco31I	GGTCTC	1 cut(s)	170
Eco47I	GGWCC	1 cut(s)	328
Eco57I	CTGAAG	1 cut(s)	67
Eco88I	CYCGRG	1 cut(s)	436
EcoNI	CCTNNNNNAGG	1 cut(s)	335
EcoO109I	RGGNCCY	1 cut(s)	328
EcoRII	CCWGG	2 cut(s)	160, 513
EcoT14I	CCWWGG	1 cut(s)	209
ErhI	CCWWGG	1 cut(s)	209
FaeI	CATG	1 cut(s)	213
FaiI	YATR	2 cut(s)	12, 211
FatI	CATG	1 cut(s)	209
FauI	CCCGC	1 cut(s)	487
Fnu4HI	GCNGC	1 cut(s)	274
FokI	GGATG	2 cut(s)	83, 222
Fsp4HI	GCNGC	1 cut(s)	274
FspBI	CTAG	2 cut(s)	59, 368
GluI	GCNGC	1 cut(s)	274
HapII	CCGG	2 cut(s)	102, 437
Hin1II	CATG	1 cut(s)	213
HincII	GTYRAC	2 cut(s)	445, 520
HindII	GTYRAC	2 cut(s)	445, 520
HinfI	GANTC	2 cut(s)	50, 441
HpaII	CCGG	2 cut(s)	102, 437
HphI	GGTGA	2 cut(s)	127, 353
Hpy166II	GTNNAC	4 cut(s)	8, 373, 445, 520
Hpy188I	TCNGA	2 cut(s)	423, 490
Hpy8I	GTNNAC	4 cut(s)	8, 373, 445, 520
HpyAV	CCTTC	1 cut(s)	263
HpyCH4III	ACNGT	1 cut(s)	262
HpyCH4V	TGCA	1 cut(s)	8
HpyF3I	CTNAG	4 cut(s)	69, 249, 265, 422
Hsp92II	CATG	1 cut(s)	213
Kzo9I	GATC	1 cut(s)	470
Lsp1109I	GCAGC	1 cut(s)	285
LweI	GCATC	2 cut(s)	189, 204
MaeI	CTAG	2 cut(s)	59, 368
MaeIII	GTNAC	2 cut(s)	133, 292
MalI	GATC	1 cut(s)	472
MboI	GATC	1 cut(s)	470
MboII	GAAGA	4 cut(s)	70, 73, 408, 503
MflI	RGATCY	1 cut(s)	470
MhlI	GDGCHC	1 cut(s)	10
MluCI	AATT	5 cut(s)	156, 399, 483, 531, 551
MlyI	GAGTC	2 cut(s)	44, 450
MmeI	TCCRAC	1 cut(s)	343
MnlI	CCTC	3 cut(s)	121, 185, 287
MroXI	GAANNNNTTC	1 cut(s)	495
MseI	TTAA	2 cut(s)	257, 333
MslI	CAYNNNNRTG	1 cut(s)	240
MspA1I	CMGCKG	2 cut(s)	68, 87
MspI	CCGG	2 cut(s)	102, 437
MspR9I	CCNGG	5 cut(s)	102, 162, 437, 438, 515
Mva1269I	GAATGC	1 cut(s)	540
MvaI	CCWGG	2 cut(s)	162, 515
NciI	CCSGG	3 cut(s)	102, 437, 438
NcoI	CCATGG	1 cut(s)	209
NdeII	GATC	1 cut(s)	470
NheI	GCTAGC	1 cut(s)	58
NlaIII	CATG	1 cut(s)	213
NlaIV	GGNNCC	1 cut(s)	472
NmeAIII	GCCGAG	1 cut(s)	156
NmuCI	GTSAC	2 cut(s)	133, 292
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PctI	GAATGC	1 cut(s)	540
PdmI	GAANNNNTTC	1 cut(s)	495
PkrI	GCNGC	1 cut(s)	275
PleI	GAGTC	2 cut(s)	44, 449
PpsI	GAGTC	2 cut(s)	44, 449
PpuMI	RGGWCCY	1 cut(s)	328
Psp5II	RGGWCCY	1 cut(s)	328
Psp6I	CCWGG	2 cut(s)	160, 513
PspGI	CCWGG	2 cut(s)	160, 513
PspN4I	GGNNCC	1 cut(s)	472
PspPI	GGNCC	1 cut(s)	328
PspPPI	RGGWCCY	1 cut(s)	328
PsuI	RGATCY	1 cut(s)	470
PvuII	CAGCTG	1 cut(s)	68
RseI	CAYNNNNRTG	1 cut(s)	240
SaqAI	TTAA	2 cut(s)	257, 333
SatI	GCNGC	1 cut(s)	274
Sau3AI	GATC	1 cut(s)	470
Sau96I	GGNCC	1 cut(s)	328
SchI	GAGTC	2 cut(s)	44, 450
ScrFI	CCNGG	5 cut(s)	102, 162, 437, 438, 515
SduI	GDGCHC	1 cut(s)	10
SetI	ASST	8 cut(s)	60, 70, 113, 166, 318, 330, 519, 550
SfaNI	GCATC	2 cut(s)	189, 204
SinI	GGWCC	1 cut(s)	328
SmaI	CCCGGG	1 cut(s)	438
SmiMI	CAYNNNNRTG	1 cut(s)	240
SpeI	ACTAGT	1 cut(s)	367
Sse9I	AATT	5 cut(s)	156, 399, 483, 531, 551
SsiI	CCGC	2 cut(s)	87, 480
SspMI	CTAG	2 cut(s)	59, 368
StyD4I	CCNGG	5 cut(s)	100, 160, 435, 436, 513
StyI	CCWWGG	1 cut(s)	209
TaaI	ACNGT	1 cut(s)	262
TaqI	TCGA	1 cut(s)	198
TasI	AATT	5 cut(s)	156, 399, 483, 531, 551
Tru1I	TTAA	2 cut(s)	257, 333
Tru9I	TTAA	2 cut(s)	257, 333
TscAI	CASTG	1 cut(s)	253
TseFI	GTSAC	2 cut(s)	133, 292
TseI	GCWGC	1 cut(s)	273
Tsp45I	GTSAC	2 cut(s)	133, 292
TspMI	CCCGGG	1 cut(s)	436
TspRI	CASTG	1 cut(s)	253
VneI	GTGCAC	1 cut(s)	6
VpaK11BI	GGWCC	1 cut(s)	328
XagI	CCTNNNNNAGG	1 cut(s)	335
XapI	RAATTY	2 cut(s)	156, 551
XmaI	CCCGGG	1 cut(s)	436
XmnI	GAANNNNTTC	1 cut(s)	495
XspI	CTAG	2 cut(s)	59, 368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.