Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004532

Physical Location & Seq

Genomic Coordinates Reverse (-)

34500474 .. 34501218

Gene Structure 745 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1252000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 654 bp

ATGGCAAACCAAATCTTGCTGTTAGCTACTTTCCTAGCCATGACATGTTCTCTGGTTGTTGCTTTTGAGCCTGCTCCACTGCAAGATTTCTGTGTTGCTGACGCTACTAGCTCAGTAACAAGAGTTAATGGGTTACCTTGCTTGGACTCCAAGCTAGCAACAGCTGAGCATTTCTTCTTCAGCGGACTTCACATCCCGGGCAACACCTCAAACCCTGTTGGCGGAAAAGTCACCCCAGTCAATGTGGCTCAGATTCCAGGACTCAACACCCTCGGCATCTCACTCGCTCGCATTGACTACGCACCAATGGGTGTTATCCCTCCCCACACCCATCCTCGCGCCACCGAGATTCTGACGGTCTTAAAAGGCAAGCTCAATGTTGGGTTTGTGACCTCAAACCCCGAGAACAAGCTCATCTCGAAGGTCCTTAAGAAGGGTGATGTGTTTGTTTTCCCTGTTGGACTAGTTCACTATCAGCAAAACCTGGGTTCAGGAACTGCCATTTCTCTATCTTCTTTGAGCAGCCAAAACCCTGGAGTCAACACCATTGCCAATGTTGTTTTTGGGTCCAATCCTGCTATTTCCGAGGATGTTTTGGCCAAGTCTTTCCAAGTGGAACAATCGGTAATTAGGTATCTTCAAGCAAAATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006810 GO:0008150 GO:0009506 GO:0009987 GO:0010496 GO:0010497 GO:0030054 GO:0030312 GO:0044464 GO:0050789 GO:0050793 GO:0051179 GO:0051234 GO:0051239 GO:0055044 GO:0065007 GO:0071944 GO:2000026 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_1

Protein Analysis

218

Amino Acids

22.96

Weight (kDa)

8.47

Isoelectric Point (pI)

31.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cupin_1	PF00190	62 - 209	1.3e-50	Cupin
Cupin_2	PF07883	98 - 167	3e-09	Cupin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004532)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g10240 FvH4_3g10730
malus_domestica	MD05G1269200.v1.1 MD05G1271400.v1.1 MD05G1271500.v1.1 MD10G1248000.v1.1 MD10G1251900.v1.1 MD10G1252000.v1.1 MD14G1081700.v1.1
prunus_persica	Prupe.4G091500_v2.0.a1 Prupe.4G095500_v2.0.a1 Prupe.4G095600_v2.0.a1
pyrus_communis	pycom10g20550 pycom10g20790
rosa_chinensis	RchiOBHm_Chr5g0016481 RchiOBHm_Chr5g0017421
rosa_laevigata	RLG00000032286
rosa_multiflora	Rmu_co8334417.1_g000001 Rmu_sc0001235.1_g000024 Rmu_sc0007392.1_g000005 Rmu_sc0019163.1_g000002
rosa_roxburghii	Rroxscaffold_1G00059970
rosa_rugosa	Rorug05G0032500 Rorug05G0035300
rosa_samantha	Rh5AG126500 Rh5AG130000 Rh5BG125300 Rh5CG137900 Rh5DG125500 Rh5DG130300
rosa_wichuraiana	Rw5G010960 Rw5G011230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	339
AciI	CCGC	2 cut(s)	183, 222
AcoI	YGGCCR	1 cut(s)	597
AcsI	RAATTY	1 cut(s)	647
AcuI	CTGAAG	1 cut(s)	163
AfiI	CCNNNNNNNGG	2 cut(s)	221, 433
AflII	CTTAAG	1 cut(s)	428
AflIII	ACRYGT	1 cut(s)	44
AgsI	TTSAA	1 cut(s)	641
AhlI	ACTAGT	1 cut(s)	463
AjnI	CCWGG	3 cut(s)	256, 483, 532
AluBI	AGCT	6 cut(s)	26, 111, 154, 164, 373, 412
AluI	AGCT	6 cut(s)	26, 111, 154, 164, 373, 412
AlwNI	CAGNNNCTG	1 cut(s)	497
Ama87I	CYCGRG	2 cut(s)	196, 401
AoxI	GGCC	1 cut(s)	597
ApeKI	GCWGC	1 cut(s)	522
ApoI	RAATTY	1 cut(s)	647
AspLEI	GCGC	1 cut(s)	341
AspS9I	GGNCC	2 cut(s)	424, 567
AsuC2I	CCSGG	2 cut(s)	197, 198
AsuHPI	GGTGA	2 cut(s)	223, 449
AsuNHI	GCTAGC	1 cut(s)	154
AvaI	CYCGRG	2 cut(s)	196, 401
AvaII	GGWCC	2 cut(s)	424, 567
BalI	TGGCCA	1 cut(s)	599
BbvI	GCAGC	1 cut(s)	534
BccI	CCATC	1 cut(s)	339
BcgI	CGANNNNNNTGC	2 cut(s)	265, 299
BciT130I	CCWGG	3 cut(s)	258, 485, 534
BcnI	CCSGG	2 cut(s)	197, 198
BcuI	ACTAGT	1 cut(s)	463
BfaI	CTAG	4 cut(s)	35, 108, 155, 464
BfrI	CTTAAG	1 cut(s)	428
BisI	GCNGC	1 cut(s)	523
BlpI	GCTNAGC	1 cut(s)	165
BlsI	GCNGC	1 cut(s)	524
Bme1390I	CCNGG	5 cut(s)	197, 198, 258, 485, 534
Bme18I	GGWCC	2 cut(s)	424, 567
BmeT110I	CYCGRG	2 cut(s)	196, 401
BmgT120I	GGNCC	2 cut(s)	424, 567
BmiI	GGNNCC	1 cut(s)	568
BmrFI	CCNGG	5 cut(s)	197, 198, 258, 485, 534
BmrI	ACTGGG	1 cut(s)	230
BmsI	GCATC	1 cut(s)	285
BmtI	GCTAGC	1 cut(s)	158
BmuI	ACTGGG	1 cut(s)	230
BpmI	CTGGAG	1 cut(s)	555
Bpu1102I	GCTNAGC	1 cut(s)	165
BpuMI	CCSGG	2 cut(s)	197, 198
BsaJI	CCNNGG	5 cut(s)	196, 271, 484, 532, 585
Bsc4I	CCNNNNNNNGG	2 cut(s)	221, 433
Bse1I	ACTGG	1 cut(s)	236
Bse3DI	GCAATG	1 cut(s)	546
BseBI	CCWGG	3 cut(s)	258, 485, 534
BseDI	CCNNGG	5 cut(s)	196, 271, 484, 532, 585
BseGI	GGATG	3 cut(s)	192, 331, 595
BseLI	CCNNNNNNNGG	2 cut(s)	221, 433
BseMI	GCAATG	1 cut(s)	546
BseMII	CTCAG	3 cut(s)	126, 156, 263
BseNI	ACTGG	1 cut(s)	236
BseXI	GCAGC	1 cut(s)	534
Bsh1236I	CGCG	1 cut(s)	339
BshFI	GGCC	1 cut(s)	599
BsiHKCI	CYCGRG	2 cut(s)	196, 401
BsiSI	CCGG	1 cut(s)	197
BslI	CCNNNNNNNGG	2 cut(s)	221, 433
BsnI	GGCC	1 cut(s)	599
BsoBI	CYCGRG	2 cut(s)	196, 401
Bsp1720I	GCTNAGC	1 cut(s)	165
BspACI	CCGC	2 cut(s)	183, 222
BspANI	GGCC	1 cut(s)	599
BspCNI	CTCAG	3 cut(s)	125, 157, 262
BspFNI	CGCG	1 cut(s)	339
BspLI	GGNNCC	1 cut(s)	568
BspOI	GCTAGC	1 cut(s)	158
BspTI	CTTAAG	1 cut(s)	428
BsrDI	GCAATG	1 cut(s)	546
BsrI	ACTGG	1 cut(s)	236
BssECI	CCNNGG	5 cut(s)	196, 271, 484, 532, 585
Bst2UI	CCWGG	3 cut(s)	258, 485, 534
Bst4CI	ACNGT	1 cut(s)	358
BstAFI	CTTAAG	1 cut(s)	428
BstC8I	GCNNGC	4 cut(s)	72, 156, 289, 371
BstDEI	CTNAG	3 cut(s)	112, 165, 249
BstEII	GGTNACC	1 cut(s)	132
BstENI	CCTNNNNNAGG	1 cut(s)	431
BstF5I	GGATG	3 cut(s)	192, 331, 595
BstFNI	CGCG	1 cut(s)	339
BstHHI	GCGC	1 cut(s)	341
BstNI	CCWGG	3 cut(s)	258, 485, 534
BstNSI	RCATGY	1 cut(s)	48
BstPI	GGTNACC	1 cut(s)	132
BstSCI	CCNGG	5 cut(s)	195, 196, 256, 483, 532
BstUI	CGCG	1 cut(s)	339
BstV1I	GCAGC	1 cut(s)	534
BstXI	CCANNNNNNTGG	1 cut(s)	533
BsuRI	GGCC	1 cut(s)	599
BtsCI	GGATG	3 cut(s)	192, 331, 595
BtsI	GCAGTG	1 cut(s)	77
BtsIMutI	CAGTG	1 cut(s)	77
Cac8I	GCNNGC	4 cut(s)	72, 156, 289, 371
CaiI	CAGNNNCTG	1 cut(s)	497
CfoI	GCGC	1 cut(s)	341
Cfr13I	GGNCC	2 cut(s)	424, 567
Cfr9I	CCCGGG	1 cut(s)	196
CseI	GACGC	1 cut(s)	110
CviAII	CATG	2 cut(s)	40, 45
DdeI	CTNAG	3 cut(s)	112, 165, 249
EaeI	YGGCCR	1 cut(s)	597
EciI	GGCGGA	1 cut(s)	237
Eco47I	GGWCC	2 cut(s)	424, 567
Eco57I	CTGAAG	1 cut(s)	163
Eco88I	CYCGRG	2 cut(s)	196, 401
Eco91I	GGTNACC	1 cut(s)	132
EcoNI	CCTNNNNNAGG	1 cut(s)	431
EcoO109I	RGGNCCY	1 cut(s)	424
EcoO65I	GGTNACC	1 cut(s)	132
EcoRII	CCWGG	3 cut(s)	256, 483, 532
FaeI	CATG	2 cut(s)	43, 48
FaiI	YATR	2 cut(s)	41, 46
FatI	CATG	2 cut(s)	39, 44
Fnu4HI	GCNGC	1 cut(s)	523
FokI	GGATG	3 cut(s)	179, 318, 602
Fsp4HI	GCNGC	1 cut(s)	523
FspBI	CTAG	4 cut(s)	35, 108, 155, 464
GlaI	GCGC	1 cut(s)	340
GluI	GCNGC	1 cut(s)	523
GsuI	CTGGAG	1 cut(s)	555
HaeIII	GGCC	1 cut(s)	599
HapII	CCGG	1 cut(s)	197
HgaI	GACGC	1 cut(s)	110
HhaI	GCGC	1 cut(s)	341
Hin1II	CATG	2 cut(s)	43, 48
Hin6I	GCGC	1 cut(s)	339
HinP1I	GCGC	1 cut(s)	339
HincII	GTYRAC	1 cut(s)	541
HindII	GTYRAC	1 cut(s)	541
HinfI	GANTC	5 cut(s)	146, 253, 261, 349, 537
HpaII	CCGG	1 cut(s)	197
HphI	GGTGA	2 cut(s)	223, 449
Hpy166II	GTNNAC	2 cut(s)	469, 541
Hpy188I	TCNGA	3 cut(s)	252, 354, 586
Hpy188III	TCNNGA	2 cut(s)	418, 492
Hpy8I	GTNNAC	2 cut(s)	469, 541
HpyAV	CCTTC	2 cut(s)	415, 427
HpyCH4III	ACNGT	1 cut(s)	358
HpyCH4V	TGCA	1 cut(s)	82
HpyF3I	CTNAG	3 cut(s)	112, 165, 249
Hsp92II	CATG	2 cut(s)	43, 48
HspAI	GCGC	1 cut(s)	339
LmnI	GCTCC	1 cut(s)	79
Lsp1109I	GCAGC	1 cut(s)	534
LweI	GCATC	1 cut(s)	285
MaeI	CTAG	4 cut(s)	35, 108, 155, 464
MaeIII	GTNAC	4 cut(s)	115, 132, 229, 388
MboII	GAAGA	4 cut(s)	166, 169, 504, 629
MlsI	TGGCCA	1 cut(s)	599
MluCI	AATT	2 cut(s)	627, 647
MluNI	TGGCCA	1 cut(s)	599
MlyI	GAGTC	3 cut(s)	140, 255, 546
MmeI	TCCRAC	1 cut(s)	439
MnlI	CCTC	6 cut(s)	217, 281, 330, 345, 403, 580
Mox20I	TGGCCA	1 cut(s)	599
MscI	TGGCCA	1 cut(s)	599
MseI	TTAA	3 cut(s)	126, 362, 429
Msp20I	TGGCCA	1 cut(s)	599
MspA1I	CMGCKG	2 cut(s)	164, 183
MspCI	CTTAAG	1 cut(s)	428
MspI	CCGG	1 cut(s)	197
MspR9I	CCNGG	5 cut(s)	197, 198, 258, 485, 534
MvaI	CCWGG	3 cut(s)	258, 485, 534
MvnI	CGCG	1 cut(s)	339
NciI	CCSGG	2 cut(s)	197, 198
NheI	GCTAGC	1 cut(s)	154
NlaIII	CATG	2 cut(s)	43, 48
NlaIV	GGNNCC	1 cut(s)	568
NmeAIII	GCCGAG	1 cut(s)	252
NmuCI	GTSAC	2 cut(s)	229, 388
NspI	RCATGY	1 cut(s)	48
PciI	ACATGT	1 cut(s)	44
PfeI	GAWTC	2 cut(s)	253, 349
PfoI	TCCNGGA	1 cut(s)	256
PkrI	GCNGC	1 cut(s)	524
PleI	GAGTC	3 cut(s)	140, 255, 545
PpsI	GAGTC	3 cut(s)	140, 255, 545
PpuMI	RGGWCCY	1 cut(s)	424
PscI	ACATGT	1 cut(s)	44
Psp5II	RGGWCCY	1 cut(s)	424
Psp6I	CCWGG	3 cut(s)	256, 483, 532
PspEI	GGTNACC	1 cut(s)	132
PspGI	CCWGG	3 cut(s)	256, 483, 532
PspN4I	GGNNCC	1 cut(s)	568
PspPI	GGNCC	2 cut(s)	424, 567
PspPPI	RGGWCCY	1 cut(s)	424
PstNI	CAGNNNCTG	1 cut(s)	497
PvuII	CAGCTG	1 cut(s)	164
SaqAI	TTAA	3 cut(s)	126, 362, 429
SatI	GCNGC	1 cut(s)	523
Sau96I	GGNCC	2 cut(s)	424, 567
SchI	GAGTC	3 cut(s)	140, 255, 546
ScrFI	CCNGG	5 cut(s)	197, 198, 258, 485, 534
SfaNI	GCATC	1 cut(s)	285
SinI	GGWCC	2 cut(s)	424, 567
SmaI	CCCGGG	1 cut(s)	198
SmlI	CTYRAG	1 cut(s)	428
SmoI	CTYRAG	1 cut(s)	428
SpeI	ACTAGT	1 cut(s)	463
Sse9I	AATT	2 cut(s)	627, 647
SsiI	CCGC	2 cut(s)	183, 222
SspMI	CTAG	4 cut(s)	35, 108, 155, 464
StyD4I	CCNGG	5 cut(s)	195, 196, 256, 483, 532
TaaI	ACNGT	1 cut(s)	358
TaqI	TCGA	1 cut(s)	419
TasI	AATT	2 cut(s)	627, 647
TfiI	GAWTC	2 cut(s)	253, 349
Tru1I	TTAA	3 cut(s)	126, 362, 429
Tru9I	TTAA	3 cut(s)	126, 362, 429
TscAI	CASTG	1 cut(s)	84
TseFI	GTSAC	2 cut(s)	229, 388
TseI	GCWGC	1 cut(s)	522
Tsp45I	GTSAC	2 cut(s)	229, 388
TspMI	CCCGGG	1 cut(s)	196
TspRI	CASTG	1 cut(s)	84
Vha464I	CTTAAG	1 cut(s)	428
VpaK11BI	GGWCC	2 cut(s)	424, 567
XagI	CCTNNNNNAGG	1 cut(s)	431
XapI	RAATTY	1 cut(s)	647
XceI	RCATGY	1 cut(s)	48
XmaI	CCCGGG	1 cut(s)	196
XspI	CTAG	4 cut(s)	35, 108, 155, 464

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.