Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004532

Physical Location & Seq

Genomic Coordinates Forward (+)

34087492 .. 34088276

Gene Structure 785 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1248000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 654 bp

ATGGCAAACCAAATCCTGCTATTGACTACTTTCCTAGCCATGACATGTTCTTTGGTTGTTGCTTTCGAGCCTAGTCCGTTGCAGGATTTCTGTGTTGCTGACGCTACTAGCTCAGCAACAAGAGTGAATGGGCTACCTTGCTTGGACTCCAAGCTAGCAACAGCTGAGCATTTCTTCTTCAGTGGACTTCACATCCCGGGCAACACCTCAAACCCTGTTGGTGGAAAAGTCACCCCTGTCAATGTGGCTCAAATTCCAGGACTCAACACCCTCGGCATCTCACTCGCTCGCATTGACTACGCACCAATGGGTGTCATCCCACCCCACACTCACCCCCGTGCCACTGAGATTTTAACCGTCTTAGAAGGCAGCCTCGATGTTGGCTTCGTGACCTCAAACCCCGACAACAAGCTCATCTCAAAGGTCCTTAACAAGGGCGATGCGTTTGTGTTCCCTGTCGGACTCATTCACTATCAGAAAAATGTGGGCACTGGAATGGCCGTTTCACTCTCTTCTTTGAGCAGCCAAAACCCTGGAGTCAACACCATTGCTAATGCTGTGTTCGGATCCAATCCCCCAATTTCAGAAGATGTCCTCGCCAAGTCTTTCCAGGTTGACAAATCCGTTATTACTAGTATTCAGGCCAAATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006810 GO:0008150 GO:0009506 GO:0009987 GO:0010496 GO:0010497 GO:0030054 GO:0030312 GO:0044464 GO:0050789 GO:0050793 GO:0051179 GO:0051234 GO:0051239 GO:0055044 GO:0065007 GO:0071944 GO:2000026 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_1

Protein Analysis

218

Amino Acids

22.76

Weight (kDa)

6.17

Isoelectric Point (pI)

29.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cupin_1	PF00190	62 - 209	8e-51	Cupin
Cupin_2	PF07883	98 - 168	2.3e-09	Cupin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004532)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g10240 FvH4_3g10730
malus_domestica	MD05G1269200.v1.1 MD05G1271400.v1.1 MD05G1271500.v1.1 MD10G1248000.v1.1 MD10G1251900.v1.1 MD10G1252000.v1.1 MD14G1081700.v1.1
prunus_persica	Prupe.4G091500_v2.0.a1 Prupe.4G095500_v2.0.a1 Prupe.4G095600_v2.0.a1
pyrus_communis	pycom10g20550 pycom10g20790
rosa_chinensis	RchiOBHm_Chr5g0016481 RchiOBHm_Chr5g0017421
rosa_laevigata	RLG00000032286
rosa_multiflora	Rmu_co8334417.1_g000001 Rmu_sc0001235.1_g000024 Rmu_sc0007392.1_g000005 Rmu_sc0019163.1_g000002
rosa_roxburghii	Rroxscaffold_1G00059970
rosa_rugosa	Rorug05G0032500 Rorug05G0035300
rosa_samantha	Rh5AG126500 Rh5AG130000 Rh5BG125300 Rh5CG137900 Rh5DG125500 Rh5DG130300
rosa_wichuraiana	Rw5G010960 Rw5G011230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	2 cut(s)	561, 574
AcoI	YGGCCR	1 cut(s)	498
AcsI	RAATTY	2 cut(s)	252, 647
AcuI	CTGAAG	1 cut(s)	163
AfiI	CCNNNNNNNGG	2 cut(s)	221, 433
AflIII	ACRYGT	1 cut(s)	44
AhlI	ACTAGT	1 cut(s)	632
AjnI	CCWGG	3 cut(s)	256, 532, 609
AleI	CACNNNNGTG	1 cut(s)	336
AluBI	AGCT	4 cut(s)	111, 154, 164, 412
AluI	AGCT	4 cut(s)	111, 154, 164, 412
AlwI	GGATC	2 cut(s)	561, 574
Ama87I	CYCGRG	1 cut(s)	196
AoxI	GGCC	2 cut(s)	498, 642
ApeKI	GCWGC	2 cut(s)	369, 522
ApoI	RAATTY	2 cut(s)	252, 647
ArsI	GACNNNNNNTTYG	2 cut(s)	34, 66
AspS9I	GGNCC	1 cut(s)	424
AsuC2I	CCSGG	2 cut(s)	197, 198
AsuHPI	GGTGA	2 cut(s)	223, 323
AsuNHI	GCTAGC	1 cut(s)	154
AvaI	CYCGRG	1 cut(s)	196
AvaII	GGWCC	1 cut(s)	424
BaeGI	GKGCMC	1 cut(s)	491
BamHI	GGATCC	1 cut(s)	566
BbvI	GCAGC	2 cut(s)	381, 534
BceAI	ACGGC	1 cut(s)	485
BcgI	CGANNNNNNTGC	2 cut(s)	265, 299
BciT130I	CCWGG	3 cut(s)	258, 534, 611
BcnI	CCSGG	2 cut(s)	197, 198
BcuI	ACTAGT	1 cut(s)	632
BfaI	CTAG	5 cut(s)	35, 72, 108, 155, 633
BisI	GCNGC	2 cut(s)	370, 523
BlpI	GCTNAGC	2 cut(s)	112, 165
BlsI	GCNGC	2 cut(s)	371, 524
Bme1390I	CCNGG	5 cut(s)	197, 198, 258, 534, 611
Bme18I	GGWCC	1 cut(s)	424
BmeT110I	CYCGRG	1 cut(s)	196
BmgT120I	GGNCC	1 cut(s)	424
BmiI	GGNNCC	1 cut(s)	568
BmrFI	CCNGG	5 cut(s)	197, 198, 258, 534, 611
BmsI	GCATC	2 cut(s)	285, 430
BmtI	GCTAGC	1 cut(s)	158
BpmI	CTGGAG	1 cut(s)	555
Bpu1102I	GCTNAGC	2 cut(s)	112, 165
BpuMI	CCSGG	2 cut(s)	197, 198
BsaJI	CCNNGG	3 cut(s)	196, 271, 532
Bsc4I	CCNNNNNNNGG	2 cut(s)	221, 433
Bse1I	ACTGG	1 cut(s)	496
Bse3DI	GCAATG	1 cut(s)	546
BseBI	CCWGG	3 cut(s)	258, 534, 611
BseDI	CCNNGG	3 cut(s)	196, 271, 532
BseGI	GGATG	2 cut(s)	192, 315
BseLI	CCNNNNNNNGG	2 cut(s)	221, 433
BseMI	GCAATG	1 cut(s)	546
BseMII	CTCAG	3 cut(s)	126, 156, 336
BseNI	ACTGG	1 cut(s)	496
BseSI	GKGCMC	1 cut(s)	491
BseXI	GCAGC	2 cut(s)	381, 534
BshFI	GGCC	2 cut(s)	500, 644
BsiHKCI	CYCGRG	1 cut(s)	196
BsiSI	CCGG	1 cut(s)	197
BslI	CCNNNNNNNGG	2 cut(s)	221, 433
BsnI	GGCC	2 cut(s)	500, 644
BsoBI	CYCGRG	1 cut(s)	196
Bsp1286I	GDGCHC	1 cut(s)	491
Bsp143I	GATC	1 cut(s)	566
Bsp1720I	GCTNAGC	2 cut(s)	112, 165
BspANI	GGCC	2 cut(s)	500, 644
BspCNI	CTCAG	3 cut(s)	125, 157, 337
BspLI	GGNNCC	1 cut(s)	568
BspOI	GCTAGC	1 cut(s)	158
BspPI	GGATC	2 cut(s)	561, 574
BsrDI	GCAATG	1 cut(s)	546
BsrI	ACTGG	1 cut(s)	496
BssECI	CCNNGG	3 cut(s)	196, 271, 532
BssMI	GATC	1 cut(s)	566
Bst2UI	CCWGG	3 cut(s)	258, 534, 611
Bst4CI	ACNGT	1 cut(s)	358
Bst6I	CTCTTC	1 cut(s)	517
BstC8I	GCNNGC	2 cut(s)	156, 289
BstDEI	CTNAG	4 cut(s)	112, 165, 345, 361
BstENI	CCTNNNNNAGG	1 cut(s)	431
BstF5I	GGATG	2 cut(s)	192, 315
BstKTI	GATC	1 cut(s)	569
BstMBI	GATC	1 cut(s)	566
BstNI	CCWGG	3 cut(s)	258, 534, 611
BstNSI	RCATGY	1 cut(s)	48
BstSCI	CCNGG	5 cut(s)	195, 196, 256, 532, 609
BstSLI	GKGCMC	1 cut(s)	491
BstV1I	GCAGC	2 cut(s)	381, 534
BstX2I	RGATCY	1 cut(s)	566
BstXI	CCANNNNNNTGG	1 cut(s)	533
BstYI	RGATCY	1 cut(s)	566
BsuRI	GGCC	2 cut(s)	500, 644
BtgZI	GCGATG	1 cut(s)	453
BtsCI	GGATG	2 cut(s)	192, 315
BtsIMutI	CAGTG	3 cut(s)	187, 342, 489
Cac8I	GCNNGC	2 cut(s)	156, 289
Cfr13I	GGNCC	1 cut(s)	424
Cfr9I	CCCGGG	1 cut(s)	196
CseI	GACGC	1 cut(s)	110
CviAII	CATG	2 cut(s)	40, 45
DdeI	CTNAG	4 cut(s)	112, 165, 345, 361
DpnI	GATC	1 cut(s)	568
DpnII	GATC	1 cut(s)	566
EaeI	YGGCCR	1 cut(s)	498
Eam1104I	CTCTTC	1 cut(s)	517
EarI	CTCTTC	1 cut(s)	517
Eco47I	GGWCC	1 cut(s)	424
Eco57I	CTGAAG	1 cut(s)	163
Eco88I	CYCGRG	1 cut(s)	196
EcoNI	CCTNNNNNAGG	1 cut(s)	431
EcoO109I	RGGNCCY	1 cut(s)	424
EcoRII	CCWGG	3 cut(s)	256, 532, 609
FaeI	CATG	2 cut(s)	43, 48
FaiI	YATR	2 cut(s)	41, 46
FatI	CATG	2 cut(s)	39, 44
Fnu4HI	GCNGC	2 cut(s)	370, 523
FokI	GGATG	2 cut(s)	179, 302
Fsp4HI	GCNGC	2 cut(s)	370, 523
FspBI	CTAG	5 cut(s)	35, 72, 108, 155, 633
GluI	GCNGC	2 cut(s)	370, 523
GsuI	CTGGAG	1 cut(s)	555
HaeIII	GGCC	2 cut(s)	500, 644
HapII	CCGG	1 cut(s)	197
HgaI	GACGC	1 cut(s)	110
Hin1II	CATG	2 cut(s)	43, 48
HincII	GTYRAC	2 cut(s)	541, 616
HindII	GTYRAC	2 cut(s)	541, 616
HinfI	GANTC	4 cut(s)	146, 261, 462, 537
HpaII	CCGG	1 cut(s)	197
HphI	GGTGA	2 cut(s)	223, 323
Hpy166II	GTNNAC	3 cut(s)	185, 541, 616
Hpy188I	TCNGA	4 cut(s)	461, 477, 566, 586
Hpy188III	TCNNGA	1 cut(s)	388
Hpy8I	GTNNAC	3 cut(s)	185, 541, 616
HpyAV	CCTTC	1 cut(s)	359
HpyCH4III	ACNGT	1 cut(s)	358
HpyCH4V	TGCA	1 cut(s)	82
HpyF3I	CTNAG	4 cut(s)	112, 165, 345, 361
Hsp92II	CATG	2 cut(s)	43, 48
Kzo9I	GATC	1 cut(s)	566
Lsp1109I	GCAGC	2 cut(s)	381, 534
LweI	GCATC	2 cut(s)	285, 430
MaeI	CTAG	5 cut(s)	35, 72, 108, 155, 633
MaeIII	GTNAC	2 cut(s)	229, 388
MalI	GATC	1 cut(s)	568
MboI	GATC	1 cut(s)	566
MboII	GAAGA	4 cut(s)	166, 169, 504, 599
MflI	RGATCY	1 cut(s)	566
MhlI	GDGCHC	1 cut(s)	491
MluCI	AATT	3 cut(s)	252, 579, 647
MlyI	GAGTC	4 cut(s)	140, 255, 456, 546
MmeI	TCCRAC	1 cut(s)	439
MnlI	CCTC	5 cut(s)	217, 281, 383, 403, 605
MseI	TTAA	2 cut(s)	353, 429
MslI	CAYNNNNRTG	2 cut(s)	336, 494
MspA1I	CMGCKG	1 cut(s)	164
MspI	CCGG	1 cut(s)	197
MspR9I	CCNGG	5 cut(s)	197, 198, 258, 534, 611
MvaI	CCWGG	3 cut(s)	258, 534, 611
NciI	CCSGG	2 cut(s)	197, 198
NdeII	GATC	1 cut(s)	566
NheI	GCTAGC	1 cut(s)	154
NlaIII	CATG	2 cut(s)	43, 48
NlaIV	GGNNCC	1 cut(s)	568
NmeAIII	GCCGAG	1 cut(s)	252
NmuCI	GTSAC	2 cut(s)	229, 388
NspI	RCATGY	1 cut(s)	48
OliI	CACNNNNGTG	1 cut(s)	336
PciI	ACATGT	1 cut(s)	44
PfoI	TCCNGGA	1 cut(s)	256
PkrI	GCNGC	2 cut(s)	371, 524
PleI	GAGTC	4 cut(s)	140, 255, 456, 545
PpsI	GAGTC	4 cut(s)	140, 255, 456, 545
PpuMI	RGGWCCY	1 cut(s)	424
PscI	ACATGT	1 cut(s)	44
Psp5II	RGGWCCY	1 cut(s)	424
Psp6I	CCWGG	3 cut(s)	256, 532, 609
PspGI	CCWGG	3 cut(s)	256, 532, 609
PspN4I	GGNNCC	1 cut(s)	568
PspPI	GGNCC	1 cut(s)	424
PspPPI	RGGWCCY	1 cut(s)	424
PsuI	RGATCY	1 cut(s)	566
PvuII	CAGCTG	1 cut(s)	164
RseI	CAYNNNNRTG	2 cut(s)	336, 494
SaqAI	TTAA	2 cut(s)	353, 429
SatI	GCNGC	2 cut(s)	370, 523
Sau3AI	GATC	1 cut(s)	566
Sau96I	GGNCC	1 cut(s)	424
SchI	GAGTC	4 cut(s)	140, 255, 456, 546
ScrFI	CCNGG	5 cut(s)	197, 198, 258, 534, 611
SduI	GDGCHC	1 cut(s)	491
SetI	ASST	9 cut(s)	113, 139, 156, 166, 209, 395, 414, 426, 615
SfaNI	GCATC	2 cut(s)	285, 430
SinI	GGWCC	1 cut(s)	424
SmaI	CCCGGG	1 cut(s)	198
SmiMI	CAYNNNNRTG	2 cut(s)	336, 494
SpeI	ACTAGT	1 cut(s)	632
Sse9I	AATT	3 cut(s)	252, 579, 647
SspMI	CTAG	5 cut(s)	35, 72, 108, 155, 633
StyD4I	CCNGG	5 cut(s)	195, 196, 256, 532, 609
TaaI	ACNGT	1 cut(s)	358
TaqI	TCGA	2 cut(s)	66, 375
TasI	AATT	3 cut(s)	252, 579, 647
Tru1I	TTAA	2 cut(s)	353, 429
Tru9I	TTAA	2 cut(s)	353, 429
TscAI	CASTG	3 cut(s)	187, 349, 496
TseFI	GTSAC	2 cut(s)	229, 388
TseI	GCWGC	2 cut(s)	369, 522
Tsp45I	GTSAC	2 cut(s)	229, 388
TspGWI	ACGGA	2 cut(s)	66, 613
TspMI	CCCGGG	1 cut(s)	196
TspRI	CASTG	3 cut(s)	187, 349, 496
VpaK11BI	GGWCC	1 cut(s)	424
XagI	CCTNNNNNAGG	1 cut(s)	431
XapI	RAATTY	2 cut(s)	252, 647
XceI	RCATGY	1 cut(s)	48
XmaI	CCCGGG	1 cut(s)	196
XspI	CTAG	5 cut(s)	35, 72, 108, 155, 633

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.