Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008040

Physical Location & Seq

Genomic Coordinates Reverse (-)

43829914 .. 43833075

Gene Structure 3162 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1306100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1143 bp

ATGTTGAACTCAAACCACCACCAACCCCCGTCTCCCTCCGACGCTTCGTCGCCGCTTTTAGGCCAATCGATGGCCGACAACCTCGTCCGCAGCCGGCTGCTCATGCGTCAGCCGCCGCGCCCTCTTCGCGGCGCGGCTCGCTTCCTGCGTCGCGCCAGCAGCCGACGCATGATGCTGCGCGAGCCGTCGGTCCGGGTACGGGAGGCGGCGGCGGAGCAGCTGGAGGAGCGCCAGAGTGACTGGGCCTACTCTCGGCCGATTATCGTGTTGGACCTCTTGTGGAACGCCGGGCTCCTTGGGATTGCCGTCGGGGTGCTGTCGATGAGCTGGAAAGAGGCGCCTTCGGTGCCTTTGAGGACTTGGATTGGCGGGTACGCGTTTCAGTGCCTGGTCCATATGGTCTGCGTGGGGGTGGAGTGCAAGCGGCGGAGGCGCGGAGGTCAAGCGGCGGCGGAAGCGAGTTCCGGGTGGGAAAATTCATCTGCGTCTGGGAGTGATGATGGTGAGGACTATGGAGTTGAGCAGAGCGCCGACTACACTGTCACCAGTGTTGCAAAGCATTTGGAGTCTGCAAATACGGTGTTTTCGTTTATCTGGTGGCTTCTTGGGTTCTACTGGGTGACTGCTGGCGGTGAAAGTTTGACACGAGATTCCCCACAGTTATACTGGATTTGTATCACTTTTCTTGCTTTGGATGTTGTCTTCGTAGTCATCTGCGTTGCTGTCGTGTCTCTCATTGGTATTGCCGTTTGCTTCTGTCTCCCCTGCATCATTGCCATATTGTATGTTGTGACAGATCAGGAAGGAGCAACAAAGGAAGAGATCGATCATTTGCCAAAGTACAAGTTCCACAAGATACCTGATTTTGACAAAGTTAATGGTGAGATCCTAGAATCTTCTGGAGGAGTAATGACGGAAAGCGACACTACTACACCTACTGAACATGTTCTTTCTGCAGAGGATGCTGAATGTTGCATCTGCCTTTGTGCCTACGAGGATGGAACTGAACTGCGTGAACTTCCATGCTGTCACCACTTTCACTGCACCTGCATAGACAAATGGTTACAGATCAATGCCACCTGCCCACTCTGCAAGTTCAACATTTTGAAGTCCGTTAGCCAAAGCGGTAGTGAAGAAGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

381

Amino Acids

42.06

Weight (kDa)

5.35

Isoelectric Point (pI)

61.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	323 - 365	7.2e-13	Ring finger domain
zf-RING_11	PF17123	323 - 351	2e-06	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008040)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61180
fragaria_vesca	FvH4_3g08230
malus_domestica	MD01G1049700.v1.1 MD05G1306100.v1.1 MD07G1126700.v1.1 MD07G1126800.v1.1 MD10G1285100.v1.1
prunus_persica	Prupe.2G157200_v2.0.a1 Prupe.4G056500_v2.0.a1
pyrus_communis	pycom01g07470 pycom05g28320 pycom07g12230 pycom07g12310 pycom10g23820
rosa_chinensis	RchiOBHm_Chr5g0009921
rosa_laevigata	RLG00000031710
rosa_multiflora	Rmu_sc0001709.1_g000014
rosa_roxburghii	Rroxscaffold_1G00066260
rosa_rugosa	Rorug04G0446900
rosa_samantha	Rh5AG078500 Rh5BG073400 Rh5CG085800 Rh5DG073900
rosa_wichuraiana	Rw5G007220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1055, 1088
AasI	GACNNNNNNGTC	2 cut(s)	83, 539
Acc36I	ACCTGC	2 cut(s)	1055, 1088
AccB1I	GGYRCC	2 cut(s)	337, 346
AccB7I	CCANNNNNTGG	1 cut(s)	70
AccII	CGCG	7 cut(s)	118, 129, 134, 153, 180, 377, 435
AclWI	GGATC	1 cut(s)	880
AcoI	YGGCCR	2 cut(s)	72, 254
AcsI	RAATTY	1 cut(s)	475
AcyI	GRCGYC	1 cut(s)	338
AfaI	GTAC	3 cut(s)	198, 374, 842
AfiI	CCNNNNNNNGG	5 cut(s)	59, 70, 128, 199, 252
AflIII	ACRYGT	2 cut(s)	375, 943
AgsI	TTSAA	3 cut(s)	7, 1099, 1108
AhdI	GACNNNNNGTC	1 cut(s)	46
AjnI	CCWGG	1 cut(s)	387
AluBI	AGCT	2 cut(s)	220, 327
AluI	AGCT	2 cut(s)	220, 327
Alw26I	GTCTC	3 cut(s)	36, 735, 764
AlwI	GGATC	1 cut(s)	880
AlwNI	CAGNNNCTG	1 cut(s)	388
AoxI	GGCC	4 cut(s)	61, 72, 243, 254
ApeKI	GCWGC	5 cut(s)	90, 97, 159, 175, 217
ApoI	RAATTY	1 cut(s)	475
Asp700I	GAANNNNTTC	1 cut(s)	945
AspLEI	GCGC	8 cut(s)	120, 134, 155, 180, 231, 340, 435, 530
AspS9I	GGNCC	4 cut(s)	190, 243, 271, 391
AsuC2I	CCSGG	3 cut(s)	194, 289, 466
AsuHPI	GGTGA	6 cut(s)	515, 535, 631, 644, 893, 1022
AvaII	GGWCC	3 cut(s)	190, 271, 391
BanI	GGYRCC	2 cut(s)	337, 346
BanII	GRGCYC	1 cut(s)	294
BauI	CACGAG	1 cut(s)	645
BbsI	GAAGAC	1 cut(s)	694
BbvI	GCAGC	5 cut(s)	84, 102, 162, 171, 229
BccI	CCATC	3 cut(s)	64, 494, 992
BceAI	ACGGC	3 cut(s)	169, 290, 731
BciT130I	CCWGG	1 cut(s)	389
BcnI	CCSGG	3 cut(s)	194, 289, 466
BcoDI	GTCTC	3 cut(s)	36, 735, 764
BfaI	CTAG	1 cut(s)	890
BfmI	CTRYAG	1 cut(s)	954
BfoI	RGCGCY	3 cut(s)	232, 341, 531
BfuAI	ACCTGC	2 cut(s)	1055, 1088
Bme1390I	CCNGG	4 cut(s)	194, 289, 389, 466
Bme18I	GGWCC	3 cut(s)	190, 271, 391
BmeRI	GACNNNNNGTC	1 cut(s)	46
BmgT120I	GGNCC	4 cut(s)	190, 243, 271, 391
BmiI	GGNNCC	3 cut(s)	293, 339, 348
BmrFI	CCNGG	4 cut(s)	194, 289, 389, 466
BmrI	ACTGGG	2 cut(s)	250, 625
BmsI	GCATC	4 cut(s)	162, 777, 952, 984
BmuI	ACTGGG	2 cut(s)	250, 625
BpiI	GAAGAC	1 cut(s)	694
BpmI	CTGGAG	2 cut(s)	242, 921
BpuMI	CCSGG	3 cut(s)	194, 289, 466
Bsa29I	ATCGAT	2 cut(s)	68, 825
BsaBI	GATNNNNATC	1 cut(s)	674
BsaHI	GRCGYC	1 cut(s)	338
BsaJI	CCNNGG	1 cut(s)	295
Bsc4I	CCNNNNNNNGG	5 cut(s)	59, 70, 128, 199, 252
Bse118I	RCCGGY	1 cut(s)	93
Bse1I	ACTGG	4 cut(s)	245, 546, 620, 671
Bse3DI	GCAATG	1 cut(s)	771
Bse8I	GATNNNNATC	1 cut(s)	674
BseBI	CCWGG	1 cut(s)	389
BseCI	ATCGAT	2 cut(s)	68, 825
BseDI	CCNNGG	1 cut(s)	295
BseGI	GGATG	3 cut(s)	700, 967, 1003
BseJI	GATNNNNATC	1 cut(s)	674
BseLI	CCNNNNNNNGG	5 cut(s)	59, 70, 128, 199, 252
BseMI	GCAATG	1 cut(s)	771
BseNI	ACTGG	4 cut(s)	245, 546, 620, 671
BseRI	GAGGAG	2 cut(s)	239, 918
BseX3I	CGGCCG	1 cut(s)	254
BseXI	GCAGC	5 cut(s)	84, 102, 162, 171, 229
BsgI	GTGCAG	1 cut(s)	1027
Bsh1236I	CGCG	7 cut(s)	118, 129, 134, 153, 180, 377, 435
Bsh1285I	CGRYCG	1 cut(s)	257
BshFI	GGCC	4 cut(s)	63, 74, 245, 256
BshNI	GGYRCC	2 cut(s)	337, 346
BshVI	ATCGAT	2 cut(s)	68, 825
BsiEI	CGRYCG	1 cut(s)	257
BsiSI	CCGG	4 cut(s)	94, 193, 288, 465
BslI	CCNNNNNNNGG	5 cut(s)	59, 70, 128, 199, 252
BsmAI	GTCTC	3 cut(s)	36, 735, 764
BsmBI	CGTCTC	1 cut(s)	36
BsnI	GGCC	4 cut(s)	63, 74, 245, 256
Bsp1286I	GDGCHC	1 cut(s)	294
Bsp143I	GATC	5 cut(s)	796, 822, 826, 885, 1068
BspANI	GGCC	4 cut(s)	63, 74, 245, 256
BspDI	ATCGAT	2 cut(s)	68, 825
BspFNI	CGCG	7 cut(s)	118, 129, 134, 153, 180, 377, 435
BspLI	GGNNCC	3 cut(s)	293, 339, 348
BspMAI	CTGCAG	1 cut(s)	958
BspMI	ACCTGC	2 cut(s)	1055, 1088
BspPI	GGATC	1 cut(s)	880
BspT107I	GGYRCC	2 cut(s)	337, 346
BsrDI	GCAATG	1 cut(s)	771
BsrFI	RCCGGY	1 cut(s)	93
BsrI	ACTGG	4 cut(s)	245, 546, 620, 671
BssAI	RCCGGY	1 cut(s)	93
BssECI	CCNNGG	1 cut(s)	295
BssMI	GATC	5 cut(s)	796, 822, 826, 885, 1068
BssNI	GRCGYC	1 cut(s)	338
BssSI	CACGAG	1 cut(s)	645
BssT1I	CCWWGG	1 cut(s)	295
Bst2BI	CACGAG	1 cut(s)	645
Bst2UI	CCWGG	1 cut(s)	389
Bst4CI	ACNGT	3 cut(s)	541, 580, 660
Bst6I	CTCTTC	2 cut(s)	129, 813
BstACI	GRCGYC	1 cut(s)	338
BstAPI	GCANNNNNTGC	1 cut(s)	962
BstC8I	GCNNGC	6 cut(s)	95, 139, 157, 182, 422, 628
BstF5I	GGATG	3 cut(s)	700, 967, 1003
BstFNI	CGCG	7 cut(s)	118, 129, 134, 153, 180, 377, 435
BstH2I	RGCGCY	3 cut(s)	232, 341, 531
BstHHI	GCGC	8 cut(s)	120, 134, 155, 180, 231, 340, 435, 530
BstKTI	GATC	5 cut(s)	799, 825, 829, 888, 1071
BstMAI	GTCTC	3 cut(s)	36, 735, 764
BstMBI	GATC	5 cut(s)	796, 822, 826, 885, 1068
BstMCI	CGRYCG	1 cut(s)	257
BstNI	CCWGG	1 cut(s)	389
BstNSI	RCATGY	1 cut(s)	947
BstSCI	CCNGG	4 cut(s)	192, 287, 387, 464
BstSFI	CTRYAG	1 cut(s)	954
BstUI	CGCG	7 cut(s)	118, 129, 134, 153, 180, 377, 435
BstV1I	GCAGC	5 cut(s)	84, 102, 162, 171, 229
BstV2I	GAAGAC	1 cut(s)	694
BstX2I	RGATCY	1 cut(s)	885
BstYI	RGATCY	1 cut(s)	885
BstZI	CGGCCG	1 cut(s)	254
Bsu15I	ATCGAT	2 cut(s)	68, 825
BsuRI	GGCC	4 cut(s)	63, 74, 245, 256
BsuTUI	ATCGAT	2 cut(s)	68, 825
BtsCI	GGATG	3 cut(s)	700, 967, 1003
BtsI	GCAGTG	1 cut(s)	1039
BtsIMutI	CAGTG	4 cut(s)	389, 537, 553, 1039
BveI	ACCTGC	2 cut(s)	1055, 1088
Cac8I	GCNNGC	6 cut(s)	95, 139, 157, 182, 422, 628
CaiI	CAGNNNCTG	1 cut(s)	388
CfoI	GCGC	8 cut(s)	120, 134, 155, 180, 231, 340, 435, 530
Cfr10I	RCCGGY	1 cut(s)	93
Cfr13I	GGNCC	4 cut(s)	190, 243, 271, 391
ClaI	ATCGAT	2 cut(s)	68, 825
CpoI	CGGWCCG	1 cut(s)	190
CseI	GACGC	5 cut(s)	50, 95, 137, 174, 474
Csp6I	GTAC	3 cut(s)	197, 373, 841
CspI	CGGWCCG	1 cut(s)	190
CviAII	CATG	4 cut(s)	103, 169, 944, 1023
CviQI	GTAC	3 cut(s)	197, 373, 841
DinI	GGCGCC	1 cut(s)	339
DpnI	GATC	5 cut(s)	798, 824, 828, 887, 1070
DpnII	GATC	5 cut(s)	796, 822, 826, 885, 1068
DrdI	GACNNNNNNGTC	2 cut(s)	83, 539
DriI	GACNNNNNGTC	1 cut(s)	46
DseDI	GACNNNNNNGTC	2 cut(s)	83, 539
EaeI	YGGCCR	2 cut(s)	72, 254
EagI	CGGCCG	1 cut(s)	254
Eam1104I	CTCTTC	2 cut(s)	129, 813
Eam1105I	GACNNNNNGTC	1 cut(s)	46
EarI	CTCTTC	2 cut(s)	129, 813
EciI	GGCGGA	3 cut(s)	227, 442, 467
EclXI	CGGCCG	1 cut(s)	254
Eco130I	CCWWGG	1 cut(s)	295
Eco24I	GRGCYC	1 cut(s)	294
Eco47I	GGWCC	3 cut(s)	190, 271, 391
Eco52I	CGGCCG	1 cut(s)	254
EcoRII	CCWGG	1 cut(s)	387
EcoT14I	CCWWGG	1 cut(s)	295
EcoT38I	GRGCYC	1 cut(s)	294
EgeI	GGCGCC	1 cut(s)	339
EheI	GGCGCC	1 cut(s)	339
ErhI	CCWWGG	1 cut(s)	295
Esp3I	CGTCTC	1 cut(s)	36
FaeI	CATG	4 cut(s)	106, 172, 947, 1026
FatI	CATG	4 cut(s)	102, 168, 943, 1022
FauI	CCCGC	1 cut(s)	362
FauNDI	CATATG	1 cut(s)	396
FokI	GGATG	3 cut(s)	707, 974, 1010
FriOI	GRGCYC	1 cut(s)	294
FspBI	CTAG	1 cut(s)	890
GlaI	GCGC	8 cut(s)	119, 133, 154, 179, 230, 339, 434, 529
GsuI	CTGGAG	2 cut(s)	242, 921
HaeII	RGCGCY	3 cut(s)	232, 341, 531
HaeIII	GGCC	4 cut(s)	63, 74, 245, 256
HapII	CCGG	4 cut(s)	94, 193, 288, 465
HgaI	GACGC	5 cut(s)	50, 95, 137, 174, 474
HhaI	GCGC	8 cut(s)	120, 134, 155, 180, 231, 340, 435, 530
Hin1I	GRCGYC	1 cut(s)	338
Hin1II	CATG	4 cut(s)	106, 172, 947, 1026
Hin6I	GCGC	8 cut(s)	118, 132, 153, 178, 229, 338, 433, 528
HinP1I	GCGC	8 cut(s)	118, 132, 153, 178, 229, 338, 433, 528
HinfI	GANTC	3 cut(s)	566, 650, 893
HpaII	CCGG	4 cut(s)	94, 193, 288, 465
HphI	GGTGA	6 cut(s)	515, 535, 631, 644, 893, 1022
Hpy166II	GTNNAC	1 cut(s)	1016
Hpy188I	TCNGA	1 cut(s)	40
Hpy188III	TCNNGA	2 cut(s)	800, 900
Hpy8I	GTNNAC	1 cut(s)	1016
Hpy99I	CGWCG	6 cut(s)	44, 52, 153, 168, 190, 311
HpyAV	CCTTC	2 cut(s)	351, 797
HpyCH4III	ACNGT	3 cut(s)	541, 580, 660
HpyCH4V	TGCA	9 cut(s)	420, 554, 572, 768, 956, 975, 1044, 1050, 1092
Hsp92I	GRCGYC	1 cut(s)	338
Hsp92II	CATG	4 cut(s)	106, 172, 947, 1026
HspAI	GCGC	8 cut(s)	118, 132, 153, 178, 229, 338, 433, 528
KasI	GGCGCC	1 cut(s)	337
KroI	GCCGGC	1 cut(s)	93
KroNI	GCCGGC	1 cut(s)	95
Kzo9I	GATC	5 cut(s)	796, 822, 826, 885, 1068
LmnI	GCTCC	4 cut(s)	214, 226, 297, 806
Lsp1109I	GCAGC	5 cut(s)	84, 102, 162, 171, 229
LweI	GCATC	4 cut(s)	162, 777, 952, 984
MaeI	CTAG	1 cut(s)	890
MaeIII	GTNAC	6 cut(s)	236, 541, 619, 790, 1028, 1062
MalI	GATC	5 cut(s)	798, 824, 828, 887, 1070
MboI	GATC	5 cut(s)	796, 822, 826, 885, 1068
MboII	GAAGA	4 cut(s)	116, 694, 830, 888
MflI	RGATCY	1 cut(s)	885
MhlI	GDGCHC	1 cut(s)	294
MluCI	AATT	1 cut(s)	475
MluI	ACGCGT	1 cut(s)	375
Mly113I	GGCGCC	1 cut(s)	338
MlyI	GAGTC	1 cut(s)	575
MmeI	TCCRAC	2 cut(s)	63, 249
MroNI	GCCGGC	1 cut(s)	93
MroXI	GAANNNNTTC	1 cut(s)	945
MseI	TTAA	1 cut(s)	876
MspA1I	CMGCKG	1 cut(s)	220
MspI	CCGG	4 cut(s)	94, 193, 288, 465
MspR9I	CCNGG	4 cut(s)	194, 289, 389, 466
MvaI	CCWGG	1 cut(s)	389
MvnI	CGCG	7 cut(s)	118, 129, 134, 153, 180, 377, 435
NaeI	GCCGGC	1 cut(s)	95
NarI	GGCGCC	1 cut(s)	338
NciI	CCSGG	3 cut(s)	194, 289, 466
NdeI	CATATG	1 cut(s)	396
NdeII	GATC	5 cut(s)	796, 822, 826, 885, 1068
NgoMIV	GCCGGC	1 cut(s)	93
NlaIII	CATG	4 cut(s)	106, 172, 947, 1026
NlaIV	GGNNCC	3 cut(s)	293, 339, 348
NmeAIII	GCCGAG	1 cut(s)	232
NmuCI	GTSAC	5 cut(s)	236, 541, 619, 790, 1028
NspI	RCATGY	1 cut(s)	947
PaqCI	CACCTGC	2 cut(s)	1055, 1088
PciI	ACATGT	1 cut(s)	943
PcsI	WCGNNNNNNNCGW	2 cut(s)	145, 584
PdiI	GCCGGC	1 cut(s)	95
PdmI	GAANNNNTTC	1 cut(s)	945
PfeI	GAWTC	2 cut(s)	650, 893
PflMI	CCANNNNNTGG	1 cut(s)	70
PleI	GAGTC	1 cut(s)	574
PluTI	GGCGCC	1 cut(s)	341
PpsI	GAGTC	1 cut(s)	574
PscI	ACATGT	1 cut(s)	943
Psp6I	CCWGG	1 cut(s)	387
PspGI	CCWGG	1 cut(s)	387
PspN4I	GGNNCC	3 cut(s)	293, 339, 348
PspPI	GGNCC	4 cut(s)	190, 243, 271, 391
PstI	CTGCAG	1 cut(s)	958
PstNI	CAGNNNCTG	1 cut(s)	388
PsuI	RGATCY	1 cut(s)	885
PvuII	CAGCTG	1 cut(s)	220
RsaI	GTAC	3 cut(s)	198, 374, 842
RsaNI	GTAC	3 cut(s)	197, 373, 841
Rsr2I	CGGWCCG	1 cut(s)	190
RsrII	CGGWCCG	1 cut(s)	190
SaqAI	TTAA	1 cut(s)	876
Sau3AI	GATC	5 cut(s)	796, 822, 826, 885, 1068
Sau96I	GGNCC	4 cut(s)	190, 243, 271, 391
SchI	GAGTC	1 cut(s)	575
ScrFI	CCNGG	4 cut(s)	194, 289, 389, 466
SduI	GDGCHC	1 cut(s)	294
SetI	ASST	9 cut(s)	84, 222, 276, 329, 442, 862, 937, 1049, 1082
SfaNI	GCATC	4 cut(s)	162, 777, 952, 984
SfcI	CTRYAG	1 cut(s)	954
SfoI	GGCGCC	1 cut(s)	339
SinI	GGWCC	3 cut(s)	190, 271, 391
Sse9I	AATT	1 cut(s)	475
SspDI	GGCGCC	1 cut(s)	337
SspMI	CTAG	1 cut(s)	890
StyD4I	CCNGG	4 cut(s)	192, 287, 387, 464
StyI	CCWWGG	1 cut(s)	295
TaaI	ACNGT	3 cut(s)	541, 580, 660
TaqI	TCGA	3 cut(s)	68, 320, 825
TaqII	GACCGA	1 cut(s)	178
TasI	AATT	1 cut(s)	475
TatI	WGTACW	1 cut(s)	840
TfiI	GAWTC	2 cut(s)	650, 893
Tru1I	TTAA	1 cut(s)	876
Tru9I	TTAA	1 cut(s)	876
TscAI	CASTG	4 cut(s)	389, 544, 553, 1046
TseFI	GTSAC	5 cut(s)	236, 541, 619, 790, 1028
TseI	GCWGC	5 cut(s)	90, 97, 159, 175, 217
Tsp45I	GTSAC	5 cut(s)	236, 541, 619, 790, 1028
TspDTI	ATGAA	1 cut(s)	468
TspGWI	ACGGA	2 cut(s)	929, 1102
TspRI	CASTG	4 cut(s)	389, 544, 553, 1046
Van91I	CCANNNNNTGG	1 cut(s)	70
VpaK11BI	GGWCC	3 cut(s)	190, 271, 391
XapI	RAATTY	1 cut(s)	475
XceI	RCATGY	1 cut(s)	947
XcmI	CCANNNNNNNNNTGG	1 cut(s)	663
XmnI	GAANNNNTTC	1 cut(s)	945
XspI	CTAG	1 cut(s)	890

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.