Prupe.4G056500_v2.0.a1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0008040
Physical Location & Seq
Forward (+)
2706491 .. 2709623
3133 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G056500.1

Sequence Viewer

Length: 1143 bp
ATGTTGGGCTCAAACCTCCAACCCCCATCTTCCTCCGATGCCGCATCACCACTTCTAAGCCACTCGATCGCCGACAACCTCGTGCGGAGCCGGCGGCTCATCCGGCGCCCGCCGAGGCCTCTCCGCGGAGCAGCTCGCTTCCTACGGCGAGCCAGCAGCCGCCGTATGATGCTGCGCGAGCCGTCGGTCCGAGTCCGCGAGGCAGCGGCGGAGCAGCTGGAGGAGCGACAAACCGACTGGGCCTACTCGCGGCCCATTATCGTATTGGACCTCTTGTGGAACGCTGGGCTTCTGGGGATTGCCATTGGGGTGCTGTGGCTGAGCCGTGAAGAGAGCCCCTTGGTGCCTTTGAGGACTTGGATTGTTGGATACGCCTTTCAGTGCCTGGTCCACATGGCCTGCGTAGGCGTGGAGTGCACGCGGCGGAGGCGCGTGGGTGATGTTGCGGTGGCGGAAGAGAGTCCTCGTTGGGAAAATTCTTCTGGGTCTGGGAGTGATGATGGTGAGGACTATGGAATTGAACAGAGCGTGGACTATGCTGGCACCAGTGTTGCAAAACATTTGGAGTCAGCAAATACGGTGTTTTCGTTCATCTGGTGGATCCTTGGGTTCTACTGGGTGACTGCTGGTGGTGAAAGTTTGATAAGAGACTCCCCTCAATTGTACTGGATTTGTATCACTTTTCTTGCTTTGGATGTGGTTTTTGTTGTGATCTGCATTGCCGTTGCATCTCTCATTGGCATTGCCGTTTGCTTCTGTCTCCCCTGCATCATTGCTATCTTGTATGTTGTGACAGATCAGGAAGGAGCAACAAAGGAAGAGATTGACCATTTGCCAAAGTACAAGTTCCGCACGATATCTGATTTTGAGAAAGTTAATGGTGAGATCCAAGAATCTTTTGGAGGAATAATGACTGAATGTGATACTAATACGCCCACTGAACATGTTCTCTCCCAAGAGGATGCTGAATGTTGCATCTGCCTTTGTGCCTATGATGATGGAACTGAACTGCGTGAACTTCCTTGCCATCACCATTTCCACTGCACCTGCATAGACAAATGGCTACAGATCAATGCCACCTGCCCACTCTGCAAGTTCAACATTTTGAAGTCTGTCAGCCAAAGTGGCAGTGAAGAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

381

Amino Acids

42.47

Weight (kDa)

5.23

Isoelectric Point (pI)

69.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1055, 1088
Acc36I ACCTGC 2 cut(s) 1055, 1088
AccB1I GGYRCC 3 cut(s) 105, 343, 542
AccII CGCG 6 cut(s) 126, 177, 198, 250, 421, 432
AclWI GGATC 3 cut(s) 595, 608, 880
AcsI RAATTY 1 cut(s) 475
AcyI GRCGYC 1 cut(s) 106
AfaI GTAC 2 cut(s) 665, 842
AfiI CCNNNNNNNGG 2 cut(s) 125, 249
AflIII ACRYGT 1 cut(s) 943
AgsI TTSAA 3 cut(s) 521, 1099, 1108
AjnI CCWGG 1 cut(s) 384
AluBI AGCT 2 cut(s) 134, 217
AluI AGCT 2 cut(s) 134, 217
Alw21I GWGCWC 1 cut(s) 419
Alw26I GTCTC 2 cut(s) 642, 764
Alw44I GTGCAC 1 cut(s) 415
AlwI GGATC 3 cut(s) 595, 608, 880
AlwNI CAGNNNCTG 1 cut(s) 385
AoxI GGCC 4 cut(s) 116, 240, 251, 396
ApaLI GTGCAC 1 cut(s) 415
ApeKI GCWGC 5 cut(s) 131, 156, 172, 203, 214
ApoI RAATTY 1 cut(s) 475
Asp700I GAANNNNTTC 1 cut(s) 945
AspLEI GCGC 3 cut(s) 108, 177, 432
AspS9I GGNCC 5 cut(s) 187, 240, 252, 268, 388
AsuHPI GGTGA 7 cut(s) 39, 449, 515, 631, 644, 893, 1022
AvaII GGWCC 3 cut(s) 187, 268, 388
BaeGI GKGCMC 1 cut(s) 419
BamHI GGATCC 1 cut(s) 600
BanI GGYRCC 3 cut(s) 105, 343, 542
BanII GRGCYC 2 cut(s) 11, 338
BauI CACGAG 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 419
BbvI GCAGC 5 cut(s) 143, 159, 168, 215, 226
BccI CCATC 4 cut(s) 34, 494, 992, 1035
BceAI ACGGC 6 cut(s) 147, 161, 166, 309, 707, 731
BciT130I CCWGG 1 cut(s) 386
BciVI GTATCC 1 cut(s) 362
BcoDI GTCTC 2 cut(s) 642, 764
BfmI CTRYAG 1 cut(s) 1064
BfoI RGCGCY 1 cut(s) 109
BfuAI ACCTGC 2 cut(s) 1055, 1088
BfuI GTATCC 1 cut(s) 362
BglI GCCNNNNNGGC 1 cut(s) 1125
BlpI GCTNAGC 1 cut(s) 320
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 3 cut(s) 187, 268, 388
BmgT120I GGNCC 5 cut(s) 187, 240, 252, 268, 388
BmiI GGNNCC 5 cut(s) 89, 107, 345, 544, 602
BmrFI CCNGG 1 cut(s) 386
BmrI ACTGGG 2 cut(s) 247, 625
BmsI GCATC 7 cut(s) 28, 53, 159, 737, 777, 952, 984
BmuI ACTGGG 2 cut(s) 247, 625
BpmI CTGGAG 1 cut(s) 239
Bpu1102I GCTNAGC 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 674
BsaHI GRCGYC 1 cut(s) 106
BsaJI CCNNGG 4 cut(s) 113, 124, 339, 604
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 249
Bse118I RCCGGY 1 cut(s) 90
Bse1I ACTGG 4 cut(s) 242, 546, 620, 671
Bse3DI GCAATG 3 cut(s) 717, 741, 771
Bse8I GATNNNNATC 1 cut(s) 674
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 4 cut(s) 113, 124, 339, 604
BseGI GGATG 3 cut(s) 99, 700, 967
BseJI GATNNNNATC 1 cut(s) 674
BseLI CCNNNNNNNGG 2 cut(s) 125, 249
BseMI GCAATG 3 cut(s) 717, 741, 771
BseMII CTCAG 1 cut(s) 311
BseNI ACTGG 4 cut(s) 242, 546, 620, 671
BseRI GAGGAG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 5 cut(s) 143, 159, 168, 215, 226
BseYI CCCAGC 1 cut(s) 284
BsgI GTGCAG 1 cut(s) 1027
Bsh1236I CGCG 6 cut(s) 126, 177, 198, 250, 421, 432
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 4 cut(s) 118, 242, 253, 398
BshNI GGYRCC 3 cut(s) 105, 343, 542
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 419
BsiSI CCGG 2 cut(s) 91, 103
BslI CCNNNNNNNGG 2 cut(s) 125, 249
BsmAI GTCTC 2 cut(s) 642, 764
BsnI GGCC 4 cut(s) 118, 242, 253, 398
Bsp1286I GDGCHC 3 cut(s) 11, 338, 419
Bsp143I GATC 6 cut(s) 66, 600, 711, 796, 885, 1068
Bsp1720I GCTNAGC 1 cut(s) 320
BspANI GGCC 4 cut(s) 118, 242, 253, 398
BspCNI CTCAG 1 cut(s) 312
BspFNI CGCG 6 cut(s) 126, 177, 198, 250, 421, 432
BspLI GGNNCC 5 cut(s) 89, 107, 345, 544, 602
BspMI ACCTGC 2 cut(s) 1055, 1088
BspPI GGATC 3 cut(s) 595, 608, 880
BspT107I GGYRCC 3 cut(s) 105, 343, 542
BsrDI GCAATG 3 cut(s) 717, 741, 771
BsrFI RCCGGY 1 cut(s) 90
BsrI ACTGG 4 cut(s) 242, 546, 620, 671
BssAI RCCGGY 1 cut(s) 90
BssECI CCNNGG 4 cut(s) 113, 124, 339, 604
BssMI GATC 6 cut(s) 66, 600, 711, 796, 885, 1068
BssNI GRCGYC 1 cut(s) 106
BssSI CACGAG 1 cut(s) 80
BssT1I CCWWGG 2 cut(s) 339, 604
Bst2BI CACGAG 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 580
Bst6I CTCTTC 4 cut(s) 324, 450, 813, 1128
BstACI GRCGYC 1 cut(s) 106
BstC8I GCNNGC 9 cut(s) 92, 110, 136, 150, 154, 179, 400, 419, 541
BstDEI CTNAG 2 cut(s) 56, 320
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 3 cut(s) 99, 700, 967
BstFNI CGCG 6 cut(s) 126, 177, 198, 250, 421, 432
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 3 cut(s) 108, 177, 432
BstKTI GATC 6 cut(s) 69, 603, 714, 799, 888, 1071
BstMAI GTCTC 2 cut(s) 642, 764
BstMBI GATC 6 cut(s) 66, 600, 711, 796, 885, 1068
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 8 cut(s) 91, 103, 178, 223, 414, 427, 1089, 1125
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 1 cut(s) 947
BstSCI CCNGG 1 cut(s) 384
BstSFI CTRYAG 1 cut(s) 1064
BstSLI GKGCMC 1 cut(s) 419
BstUI CGCG 6 cut(s) 126, 177, 198, 250, 421, 432
BstV1I GCAGC 5 cut(s) 143, 159, 168, 215, 226
BstX2I RGATCY 2 cut(s) 600, 885
BstYI RGATCY 2 cut(s) 600, 885
BsuI GTATCC 1 cut(s) 362
BsuRI GGCC 4 cut(s) 118, 242, 253, 398
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 3 cut(s) 99, 700, 967
BtsI GCAGTG 2 cut(s) 1039, 1135
BtsIMutI CAGTG 5 cut(s) 386, 553, 936, 1039, 1135
BveI ACCTGC 2 cut(s) 1055, 1088
Cac8I GCNNGC 9 cut(s) 92, 110, 136, 150, 154, 179, 400, 419, 541
CaiI CAGNNNCTG 1 cut(s) 385
CfoI GCGC 3 cut(s) 108, 177, 432
Cfr10I RCCGGY 1 cut(s) 90
Cfr13I GGNCC 5 cut(s) 187, 240, 252, 268, 388
Cfr42I CCGCGG 1 cut(s) 127
CpoI CGGWCCG 1 cut(s) 187
Csp6I GTAC 2 cut(s) 664, 841
CspI CGGWCCG 1 cut(s) 187
CviAII CATG 2 cut(s) 394, 944
CviQI GTAC 2 cut(s) 664, 841
DdeI CTNAG 2 cut(s) 56, 320
DinI GGCGCC 1 cut(s) 107
DpnI GATC 6 cut(s) 68, 602, 713, 798, 887, 1070
DpnII GATC 6 cut(s) 66, 600, 711, 796, 885, 1068
Eam1104I CTCTTC 4 cut(s) 324, 450, 813, 1128
EarI CTCTTC 4 cut(s) 324, 450, 813, 1128
EciI GGCGGA 3 cut(s) 224, 439, 467
Eco130I CCWWGG 2 cut(s) 339, 604
Eco147I AGGCCT 1 cut(s) 118
Eco24I GRGCYC 2 cut(s) 11, 338
Eco32I GATATC 1 cut(s) 858
Eco47I GGWCC 3 cut(s) 187, 268, 388
EcoRII CCWGG 1 cut(s) 384
EcoRV GATATC 1 cut(s) 858
EcoT14I CCWWGG 2 cut(s) 339, 604
EcoT38I GRGCYC 2 cut(s) 11, 338
EgeI GGCGCC 1 cut(s) 107
EheI GGCGCC 1 cut(s) 107
ErhI CCWWGG 2 cut(s) 339, 604
FaeI CATG 2 cut(s) 397, 947
FaiI YATR 9 cut(s) 167, 395, 513, 537, 786, 945, 993, 1052, 1141
FatI CATG 2 cut(s) 393, 943
FauI CCCGC 1 cut(s) 117
FokI GGATG 3 cut(s) 86, 707, 974
FriOI GRGCYC 2 cut(s) 11, 338
GlaI GCGC 3 cut(s) 107, 176, 431
GsaI CCCAGC 1 cut(s) 288
GsuI CTGGAG 1 cut(s) 239
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 4 cut(s) 118, 242, 253, 398
HapII CCGG 2 cut(s) 91, 103
HhaI GCGC 3 cut(s) 108, 177, 432
Hin1I GRCGYC 1 cut(s) 106
Hin1II CATG 2 cut(s) 397, 947
Hin6I GCGC 3 cut(s) 106, 175, 430
HinP1I GCGC 3 cut(s) 106, 175, 430
HinfI GANTC 5 cut(s) 192, 460, 566, 650, 893
HpaII CCGG 2 cut(s) 91, 103
HphI GGTGA 7 cut(s) 39, 449, 515, 631, 644, 893, 1022
Hpy166II GTNNAC 4 cut(s) 391, 417, 532, 1016
Hpy188I TCNGA 3 cut(s) 37, 191, 862
Hpy188III TCNNGA 1 cut(s) 800
Hpy8I GTNNAC 4 cut(s) 391, 417, 532, 1016
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 1 cut(s) 797
HpyCH4III ACNGT 1 cut(s) 580
HpyCH4V TGCA 9 cut(s) 417, 554, 717, 728, 768, 975, 1044, 1050, 1092
HpyF10VI GCNNNNNNNGC 8 cut(s) 91, 103, 178, 223, 414, 427, 1089, 1125
HpyF3I CTNAG 2 cut(s) 56, 320
Hsp92I GRCGYC 1 cut(s) 106
Hsp92II CATG 2 cut(s) 397, 947
HspAI GCGC 3 cut(s) 106, 175, 430
KasI GGCGCC 1 cut(s) 105
KroI GCCGGC 1 cut(s) 90
KroNI GCCGGC 1 cut(s) 92
KspI CCGCGG 1 cut(s) 127
Kzo9I GATC 6 cut(s) 66, 600, 711, 796, 885, 1068
LmnI GCTCC 5 cut(s) 87, 128, 211, 223, 806
Lsp1109I GCAGC 5 cut(s) 143, 159, 168, 215, 226
LweI GCATC 7 cut(s) 28, 53, 159, 737, 777, 952, 984
MaeIII GTNAC 2 cut(s) 619, 790
MalI GATC 6 cut(s) 68, 602, 713, 798, 887, 1070
MboI GATC 6 cut(s) 66, 600, 711, 796, 885, 1068
MboII GAAGA 5 cut(s) 21, 341, 467, 471, 830
MfeI CAATTG 1 cut(s) 659
MflI RGATCY 2 cut(s) 600, 885
MhlI GDGCHC 3 cut(s) 11, 338, 419
MluCI AATT 3 cut(s) 475, 516, 659
Mly113I GGCGCC 1 cut(s) 106
MlyI GAGTC 4 cut(s) 201, 469, 575, 644
MmeI TCCRAC 2 cut(s) 43, 346
MroNI GCCGGC 1 cut(s) 90
MroXI GAANNNNTTC 1 cut(s) 945
MseI TTAA 1 cut(s) 876
MslI CAYNNNNRTG 1 cut(s) 308
MspA1I CMGCKG 3 cut(s) 126, 206, 217
MspI CCGG 2 cut(s) 91, 103
MspR9I CCNGG 1 cut(s) 386
MunI CAATTG 1 cut(s) 659
MvaI CCWGG 1 cut(s) 386
MvnI CGCG 6 cut(s) 126, 177, 198, 250, 421, 432
MwoI GCNNNNNNNGC 8 cut(s) 91, 103, 178, 223, 414, 427, 1089, 1125
NaeI GCCGGC 1 cut(s) 92
NarI GGCGCC 1 cut(s) 106
NdeII GATC 6 cut(s) 66, 600, 711, 796, 885, 1068
NgoMIV GCCGGC 1 cut(s) 90
NlaIII CATG 2 cut(s) 397, 947
NlaIV GGNNCC 5 cut(s) 89, 107, 345, 544, 602
NmeAIII GCCGAG 1 cut(s) 138
NmuCI GTSAC 2 cut(s) 619, 790
NspI RCATGY 1 cut(s) 947
PaqCI CACCTGC 2 cut(s) 1055, 1088
PceI AGGCCT 1 cut(s) 118
PciI ACATGT 1 cut(s) 943
PcsI WCGNNNNNNNCGW 1 cut(s) 584
PdiI GCCGGC 1 cut(s) 92
PdmI GAANNNNTTC 1 cut(s) 945
PfeI GAWTC 1 cut(s) 893
Ple19I CGATCG 1 cut(s) 69
PleI GAGTC 4 cut(s) 200, 468, 574, 644
PluTI GGCGCC 1 cut(s) 109
PpsI GAGTC 4 cut(s) 200, 468, 574, 644
PscI ACATGT 1 cut(s) 943
Psp6I CCWGG 1 cut(s) 384
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 5 cut(s) 89, 107, 345, 544, 602
PspPI GGNCC 5 cut(s) 187, 240, 252, 268, 388
PstNI CAGNNNCTG 1 cut(s) 385
PsuI RGATCY 2 cut(s) 600, 885
PvuI CGATCG 1 cut(s) 69
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 2 cut(s) 665, 842
RsaNI GTAC 2 cut(s) 664, 841
RseI CAYNNNNRTG 1 cut(s) 308
Rsr2I CGGWCCG 1 cut(s) 187
RsrII CGGWCCG 1 cut(s) 187
SacII CCGCGG 1 cut(s) 127
SaqAI TTAA 1 cut(s) 876
Sau3AI GATC 6 cut(s) 66, 600, 711, 796, 885, 1068
Sau96I GGNCC 5 cut(s) 187, 240, 252, 268, 388
SchI GAGTC 4 cut(s) 201, 469, 575, 644
ScrFI CCNGG 1 cut(s) 386
SduI GDGCHC 3 cut(s) 11, 338, 419
SetI ASST 8 cut(s) 18, 81, 136, 219, 273, 1049, 1082, 1140
SfaNI GCATC 7 cut(s) 28, 53, 159, 737, 777, 952, 984
SfcI CTRYAG 1 cut(s) 1064
SfoI GGCGCC 1 cut(s) 107
Sfr303I CCGCGG 1 cut(s) 127
SgrBI CCGCGG 1 cut(s) 127
SinI GGWCC 3 cut(s) 187, 268, 388
SmiMI CAYNNNNRTG 1 cut(s) 308
Sse9I AATT 3 cut(s) 475, 516, 659
SseBI AGGCCT 1 cut(s) 118
SspDI GGCGCC 1 cut(s) 105
StuI AGGCCT 1 cut(s) 118
StyD4I CCNGG 1 cut(s) 384
StyI CCWWGG 2 cut(s) 339, 604
TaaI ACNGT 1 cut(s) 580
TaqI TCGA 1 cut(s) 65
TaqII GACCGA 1 cut(s) 175
TasI AATT 3 cut(s) 475, 516, 659
TatI WGTACW 2 cut(s) 663, 840
TauI GCSGC 6 cut(s) 44, 97, 162, 209, 253, 424
TfiI GAWTC 1 cut(s) 893
Tru1I TTAA 1 cut(s) 876
Tru9I TTAA 1 cut(s) 876
TscAI CASTG 5 cut(s) 386, 553, 943, 1046, 1135
TseFI GTSAC 2 cut(s) 619, 790
TseI GCWGC 5 cut(s) 131, 156, 172, 203, 214
Tsp45I GTSAC 2 cut(s) 619, 790
TspDTI ATGAA 1 cut(s) 580
TspRI CASTG 5 cut(s) 386, 553, 943, 1046, 1135
VneI GTGCAC 1 cut(s) 415
VpaK11BI GGWCC 3 cut(s) 187, 268, 388
XapI RAATTY 1 cut(s) 475
XceI RCATGY 1 cut(s) 947
XcmI CCANNNNNNNNNTGG 2 cut(s) 262, 896
XmnI GAANNNNTTC 1 cut(s) 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.