Rorug04G0446900

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008040
Physical Location & Seq
Forward (+)
60262587 .. 60263263
677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0446900.1

Sequence Viewer

Length: 642 bp
ATGGCGATGAAGAGCAAAAAGCCTAGCCAGGGTCGCCAAAAGATTGTGATTGCCAAAATACCAAAGAGAAGTAACTTACAAGTCACATTCTCGAAACGTTGTTCAGGACTCTTCAAAAAGGCTAGTGAGCTCTGCACACTCTGTGGAGTTGAGATTGTGATCATAGTCTTTTCTCCGGCTAACAAGCCATTCTCTTTTGGCCATCCTAGTGTTGAGAGCCTCATCGGCCGTTTTCTTACTCCAAGCCATTTATCATTTTCCAGTAGTACTCAACAAGCTCAGTACTGCAGGGAGGTTAGCTCAAGCTTGGAGGCTGAGAAACATCGAGGAGAAGCACTTGACAAGCTGAGTGAAGACAGCGAGAGATCAAGCCATTGTTGGTGGGAAAAATCCATTGAGGAACTTGGATTGGATGAGCTTCAGACATTGAAGGCTGCGTTGGAGGAGCTCAAGAAGAATGTGACTGAAGAAGTCCACAAAATGAATGACCCTTGCAATTCTTCAGTACCATTCTTTATGATGAATGGTATTCAACTTGATCATCGTCTTTCCGAGAGAAAAGCCGTTGAGATTAATGTATCACATGGTTCCATGATCCCTCCTAGTGCCTATAGATTTGGTTATGGACATGGCTTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

213

Amino Acids

23.91

Weight (kDa)

8.7

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 19 - 66 2.4e-23 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 97
AclWI GGATC 1 cut(s) 589
AcoI YGGCCR 2 cut(s) 199, 226
AcuI CTGAAG 3 cut(s) 404, 486, 486
AdeI CACNNNGTG 1 cut(s) 143
AfaI GTAC 3 cut(s) 268, 284, 507
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 3 cut(s) 115, 430, 533
AjnI CCWGG 1 cut(s) 27
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
AluBI AGCT 7 cut(s) 130, 278, 300, 306, 346, 418, 448
AluI AGCT 7 cut(s) 130, 278, 300, 306, 346, 418, 448
Alw21I GWGCWC 2 cut(s) 132, 450
AlwI GGATC 1 cut(s) 589
AoxI GGCC 2 cut(s) 199, 226
ApeKI GCWGC 1 cut(s) 434
AseI ATTAAT 1 cut(s) 573
BalI TGGCCA 1 cut(s) 201
BanII GRGCYC 2 cut(s) 132, 450
BbsI GAAGAC 1 cut(s) 360
Bbv12I GWGCWC 2 cut(s) 132, 450
BbvI GCAGC 1 cut(s) 421
BccI CCATC 1 cut(s) 210
BceAI ACGGC 2 cut(s) 213, 548
BciT130I CCWGG 1 cut(s) 29
BclI TGATCA 2 cut(s) 159, 538
BfaI CTAG 4 cut(s) 24, 123, 207, 603
BfmI CTRYAG 2 cut(s) 286, 610
BglI GCCNNNNNGGC 1 cut(s) 225
BisI GCNGC 1 cut(s) 435
BlsI GCNGC 1 cut(s) 436
BmcAI AGTACT 2 cut(s) 268, 284
Bme1390I CCNGG 1 cut(s) 29
BmiI GGNNCC 1 cut(s) 589
BmrFI CCNGG 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 360
BplI GAGNNNNNCTC 2 cut(s) 284, 316
BpuEI CTTGAG 2 cut(s) 286, 434
BsaBI GATNNNNATC 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 28
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse1I ACTGG 1 cut(s) 261
Bse8I GATNNNNATC 1 cut(s) 158
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 2 cut(s) 202, 418
BseJI GATNNNNATC 1 cut(s) 158
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMII CTCAG 3 cut(s) 293, 306, 338
BseNI ACTGG 1 cut(s) 261
BseRI GAGGAG 2 cut(s) 342, 458
BseX3I CGGCCG 1 cut(s) 226
BseXI GCAGC 1 cut(s) 421
BsgI GTGCAG 1 cut(s) 118
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 2 cut(s) 201, 228
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 2 cut(s) 132, 450
BsiSI CCGG 1 cut(s) 176
BslI CCNNNNNNNGG 1 cut(s) 29
BsnI GGCC 2 cut(s) 201, 228
Bsp1286I GDGCHC 2 cut(s) 132, 450
Bsp143I GATC 4 cut(s) 159, 365, 538, 594
BspANI GGCC 2 cut(s) 201, 228
BspCNI CTCAG 3 cut(s) 292, 307, 339
BspLI GGNNCC 1 cut(s) 589
BspMAI CTGCAG 1 cut(s) 290
BspPI GGATC 1 cut(s) 589
BspQI GCTCTTC 1 cut(s) 5
BsrI ACTGG 1 cut(s) 261
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 4 cut(s) 159, 365, 538, 594
Bst2UI CCWGG 1 cut(s) 29
Bst6I CTCTTC 2 cut(s) 5, 116
BstDEI CTNAG 3 cut(s) 279, 315, 347
BstF5I GGATG 2 cut(s) 202, 418
BstKTI GATC 4 cut(s) 162, 368, 541, 597
BstMBI GATC 4 cut(s) 159, 365, 538, 594
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 2 cut(s) 33, 225
BstNI CCWGG 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 2 cut(s) 286, 610
BstV1I GCAGC 1 cut(s) 421
BstV2I GAAGAC 1 cut(s) 360
BstZI CGGCCG 1 cut(s) 226
BsuRI GGCC 2 cut(s) 201, 228
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 202, 418
Csp6I GTAC 3 cut(s) 267, 283, 506
CviAII CATG 3 cut(s) 584, 592, 629
CviQI GTAC 3 cut(s) 267, 283, 506
DdeI CTNAG 3 cut(s) 279, 315, 347
DpnI GATC 4 cut(s) 161, 367, 540, 596
DpnII GATC 4 cut(s) 159, 365, 538, 594
DraIII CACNNNGTG 1 cut(s) 143
EaeI YGGCCR 2 cut(s) 199, 226
EagI CGGCCG 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 5, 116
EarI CTCTTC 2 cut(s) 5, 116
Ecl136II GAGCTC 2 cut(s) 130, 448
EclXI CGGCCG 1 cut(s) 226
Eco24I GRGCYC 2 cut(s) 132, 450
Eco52I CGGCCG 1 cut(s) 226
Eco53kI GAGCTC 2 cut(s) 130, 448
Eco57I CTGAAG 3 cut(s) 404, 486, 486
EcoICRI GAGCTC 2 cut(s) 130, 448
EcoRII CCWGG 1 cut(s) 27
EcoT38I GRGCYC 2 cut(s) 132, 450
FaeI CATG 3 cut(s) 587, 595, 632
FaiI YATR 7 cut(s) 164, 518, 585, 593, 612, 624, 630
FatI CATG 3 cut(s) 583, 591, 628
FbaI TGATCA 2 cut(s) 159, 538
Fnu4HI GCNGC 1 cut(s) 435
FokI GGATG 2 cut(s) 189, 425
FriOI GRGCYC 2 cut(s) 132, 450
Fsp4HI GCNGC 1 cut(s) 435
FspBI CTAG 4 cut(s) 24, 123, 207, 603
GluI GCNGC 1 cut(s) 435
HaeIII GGCC 2 cut(s) 201, 228
HapII CCGG 1 cut(s) 176
Hin1II CATG 3 cut(s) 587, 595, 632
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 1 cut(s) 108
HpaII CCGG 1 cut(s) 176
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 3 cut(s) 423, 553, 641
Hpy188III TCNNGA 3 cut(s) 91, 105, 451
Hpy8I GTNNAC 1 cut(s) 475
HpyAV CCTTC 1 cut(s) 424
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 3 cut(s) 135, 288, 495
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 225
HpyF3I CTNAG 3 cut(s) 279, 315, 347
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 3 cut(s) 587, 595, 632
Ksp22I TGATCA 2 cut(s) 159, 538
Kzo9I GATC 4 cut(s) 159, 365, 538, 594
LguI GCTCTTC 1 cut(s) 5
LmnI GCTCC 1 cut(s) 445
LpnPI CCDG 6 cut(s) 14, 41, 90, 189, 274, 274
Lsp1109I GCAGC 1 cut(s) 421
MaeI CTAG 4 cut(s) 24, 123, 207, 603
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 3 cut(s) 71, 82, 460
MalI GATC 4 cut(s) 161, 367, 540, 596
MboI GATC 4 cut(s) 159, 365, 538, 594
MboII GAAGA 6 cut(s) 22, 103, 365, 466, 479, 492
MhlI GDGCHC 2 cut(s) 132, 450
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 1 cut(s) 496
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 1 cut(s) 102
MmeI TCCRAC 1 cut(s) 420
MnlI CCTC 7 cut(s) 230, 286, 304, 320, 391, 436, 609
Mox20I TGGCCA 1 cut(s) 201
MscI TGGCCA 1 cut(s) 201
MseI TTAA 1 cut(s) 573
MslI CAYNNNNRTG 1 cut(s) 207
Msp20I TGGCCA 1 cut(s) 201
MspI CCGG 1 cut(s) 176
MspR9I CCNGG 1 cut(s) 29
MvaI CCWGG 1 cut(s) 29
MwoI GCNNNNNNNGC 2 cut(s) 33, 225
NdeII GATC 4 cut(s) 159, 365, 538, 594
NlaIII CATG 3 cut(s) 587, 595, 632
NlaIV GGNNCC 1 cut(s) 589
NmuCI GTSAC 2 cut(s) 82, 460
PciSI GCTCTTC 1 cut(s) 5
PkrI GCNGC 1 cut(s) 436
PleI GAGTC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 102
PshBI ATTAAT 1 cut(s) 573
Psp124BI GAGCTC 2 cut(s) 132, 450
Psp1406I AACGTT 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 589
PstI CTGCAG 1 cut(s) 290
RsaI GTAC 3 cut(s) 268, 284, 507
RsaNI GTAC 3 cut(s) 267, 283, 506
RseI CAYNNNNRTG 1 cut(s) 207
SacI GAGCTC 2 cut(s) 132, 450
SapI GCTCTTC 1 cut(s) 5
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 1 cut(s) 435
Sau3AI GATC 4 cut(s) 159, 365, 538, 594
ScaI AGTACT 2 cut(s) 268, 284
SchI GAGTC 1 cut(s) 102
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 2 cut(s) 132, 450
SetI ASST 9 cut(s) 100, 132, 280, 297, 302, 308, 348, 420, 450
SfcI CTRYAG 2 cut(s) 286, 610
SmiMI CAYNNNNRTG 1 cut(s) 207
SmlI CTYRAG 2 cut(s) 301, 449
SmoI CTYRAG 2 cut(s) 301, 449
Sse9I AATT 1 cut(s) 496
SspMI CTAG 4 cut(s) 24, 123, 207, 603
SstI GAGCTC 2 cut(s) 132, 450
StyD4I CCNGG 1 cut(s) 27
TaiI ACGT 1 cut(s) 100
TaqI TCGA 2 cut(s) 92, 325
TasI AATT 1 cut(s) 496
TatI WGTACW 2 cut(s) 266, 282
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TseFI GTSAC 2 cut(s) 82, 460
TseI GCWGC 1 cut(s) 434
Tsp45I GTSAC 2 cut(s) 82, 460
TspDTI ATGAA 3 cut(s) 23, 497, 536
VspI ATTAAT 1 cut(s) 573
XspI CTAG 4 cut(s) 24, 123, 207, 603
ZrmI AGTACT 2 cut(s) 268, 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.