pycom10g23820

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008040
Physical Location & Seq
Reverse (-)
25822193 .. 25824576
2384 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23820.2

Sequence Viewer

Length: 1146 bp
ATGTTGAGCTCAAACCAATATCAACCCTCTCCCTCCGACGCCTCGTCGCCGCTTTTAGGCCACTCTATCGCGGACAACATCGTCCGGAGCCGGAGACTCATACGTCGGCCGCCGCATCCTCTCCGTGGCGCAGCTCGGTTCTTGCGACGCGCGAGCAGCCGCCGGATGATGCTGCGAGAGCCGTCGGTTAGAGTCCGTGAGGCCGCGGCGGAGCAGGTGGAGGATCGCCAGAGCGACTGGGCCTACTCGCGGCCGATTATCGTTTTGGACCTATTGTGGAACGCTGGGCTCCTGGGACTTGCCGTCGGGGTGCTGTCGCTGAGCTGGAAAGAGGAGCCTTCCGTGCCTTTGAGGACTTGGATTGGCGGGTACGCGTTTCAGTGCCTGGTGCATATGGTCTGCGTGGTCGTGGAGTGCAAGCGGAGGAGGCGCGGAGGTCAAGCGGCGGCGGGGGCGGAAGCGAGTTCTGCGTGGGAAAGTTCATCTGGGTCTGGGAGTGATGATGCTGAGGACTATGGAGTTGAGCAGAGCACAGATTACACTGCCACCAGTGTTGCAAAGAATTTGGAGTCTGCGAATACGGTATTTTCGTTTATCTGGTGGATTCTTGGGTTCTACTGGGTGACTGCTGGCGGTGAAAATTTGACAGAGGAATCTCCCCAGTTATACTGGATATGTATCACTTTTCTTGCTTTGGATGTTGTTTTTGTAGTCATCTGCGTTGTTCTTGCATCTCTCGTTGGTATTGCCGTTTGCTTCTGTCTCCCCTGCATCATTGCCATCTTGTATGTTGTGACAGATCAGGAAGGAGCAACAAAGGAAGAGATCGATCGTTTGCCAAAGTACAAGTTCCGCAAGATACCTGATTTTGACAAAGTTAATGGTGAGATCCAAGAATCTTTTGGAGGAGTAATGACTGAATGCGACACTACTACACCTACCGAAAATGTTCTCTCCCCAGAGGATGCTGAATGTTGCATCTGCCTTTGTGCCTACGAAGATGGAATTGAACTGCGTGAACTTCCTTGCTGTCACCACTTTCACTGCACCTGCATAGACAAATGGTTACAGATCAATGCCACCTGCCCACTCTGCAAGTTCAACATTTTGAAGTCCGTTAGCCAAAGCAGTAGCGAAGAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

382

Amino Acids

42.31

Weight (kDa)

5.11

Isoelectric Point (pI)

67.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 205, 1058, 1091
AasI GACNNNNNNGTC 2 cut(s) 80, 102
Acc36I ACCTGC 3 cut(s) 205, 1058, 1091
AccII CGCG 7 cut(s) 71, 150, 152, 206, 250, 374, 432
AccIII TCCGGA 1 cut(s) 84
AclWI GGATC 2 cut(s) 231, 883
AcoI YGGCCR 2 cut(s) 107, 251
AcsI RAATTY 2 cut(s) 562, 640
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 371, 845
AfiI CCNNNNNNNGG 3 cut(s) 56, 125, 249
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 3 cut(s) 1010, 1102, 1111
AhdI GACNNNNNGTC 2 cut(s) 43, 302
AjnI CCWGG 2 cut(s) 291, 384
AluBI AGCT 3 cut(s) 9, 134, 324
AluI AGCT 3 cut(s) 9, 134, 324
Alw21I GWGCWC 2 cut(s) 11, 533
Alw26I GTCTC 2 cut(s) 88, 767
AlwI GGATC 2 cut(s) 231, 883
AlwNI CAGNNNCTG 1 cut(s) 385
Aor13HI TCCGGA 1 cut(s) 84
AoxI GGCC 5 cut(s) 58, 107, 201, 240, 251
ApeKI GCWGC 3 cut(s) 131, 156, 172
ApoI RAATTY 2 cut(s) 562, 640
Asp700I GAANNNNTTC 1 cut(s) 948
AspLEI GCGC 3 cut(s) 131, 152, 432
AspS9I GGNCC 2 cut(s) 240, 268
AsuHPI GGTGA 4 cut(s) 634, 647, 896, 1025
AvaII GGWCC 1 cut(s) 268
BanII GRGCYC 2 cut(s) 11, 291
Bbv12I GWGCWC 2 cut(s) 11, 533
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 3 cut(s) 143, 159, 168
BccI CCATC 2 cut(s) 788, 995
BceAI ACGGC 3 cut(s) 166, 287, 734
BciT130I CCWGG 2 cut(s) 293, 386
BcoDI GTCTC 2 cut(s) 88, 767
BfuAI ACCTGC 3 cut(s) 205, 1058, 1091
BlpI GCTNAGC 1 cut(s) 320
Bme1390I CCNGG 2 cut(s) 293, 386
Bme18I GGWCC 1 cut(s) 268
BmeRI GACNNNNNGTC 2 cut(s) 43, 302
BmgT120I GGNCC 2 cut(s) 240, 268
BmiI GGNNCC 3 cut(s) 89, 290, 336
BmrFI CCNGG 2 cut(s) 293, 386
BmrI ACTGGG 3 cut(s) 247, 628, 655
BmsI GCATC 7 cut(s) 124, 159, 493, 740, 780, 955, 987
BmuI ACTGGG 3 cut(s) 247, 628, 655
Bpu10I CCTNAGC 1 cut(s) 507
Bpu1102I GCTNAGC 1 cut(s) 320
Bsa29I ATCGAT 1 cut(s) 828
BsaBI GATNNNNATC 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 3 cut(s) 124, 204, 292
BsaWI WCCGGW 1 cut(s) 84
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 125, 249
Bse1I ACTGG 5 cut(s) 242, 549, 623, 661, 674
Bse3DI GCAATG 1 cut(s) 774
Bse8I GATNNNNATC 1 cut(s) 677
BseAI TCCGGA 1 cut(s) 84
BseBI CCWGG 2 cut(s) 293, 386
BseCI ATCGAT 1 cut(s) 828
BseDI CCNNGG 3 cut(s) 124, 204, 292
BseGI GGATG 4 cut(s) 115, 171, 703, 970
BseJI GATNNNNATC 1 cut(s) 677
BseLI CCNNNNNNNGG 3 cut(s) 56, 125, 249
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 2 cut(s) 311, 498
BseNI ACTGG 5 cut(s) 242, 549, 623, 661, 674
BseRI GAGGAG 3 cut(s) 347, 439, 921
BseX3I CGGCCG 2 cut(s) 107, 251
BseXI GCAGC 3 cut(s) 143, 159, 168
BseYI CCCAGC 1 cut(s) 284
BsgI GTGCAG 1 cut(s) 1030
Bsh1236I CGCG 7 cut(s) 71, 150, 152, 206, 250, 374, 432
Bsh1285I CGRYCG 3 cut(s) 110, 254, 832
BshFI GGCC 5 cut(s) 60, 109, 203, 242, 253
BshVI ATCGAT 1 cut(s) 828
BsiEI CGRYCG 3 cut(s) 110, 254, 832
BsiHKAI GWGCWC 2 cut(s) 11, 533
BsiSI CCGG 3 cut(s) 85, 91, 163
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 3 cut(s) 56, 125, 249
BsmAI GTCTC 2 cut(s) 88, 767
BsmFI GGGAC 1 cut(s) 309
BsmI GAATGC 1 cut(s) 926
BsnI GGCC 5 cut(s) 60, 109, 203, 242, 253
Bsp1286I GDGCHC 3 cut(s) 11, 291, 533
Bsp13I TCCGGA 1 cut(s) 84
Bsp143I GATC 6 cut(s) 223, 799, 825, 829, 888, 1071
Bsp1720I GCTNAGC 1 cut(s) 320
BspANI GGCC 5 cut(s) 60, 109, 203, 242, 253
BspCNI CTCAG 2 cut(s) 312, 499
BspDI ATCGAT 1 cut(s) 828
BspEI TCCGGA 1 cut(s) 84
BspFNI CGCG 7 cut(s) 71, 150, 152, 206, 250, 374, 432
BspLI GGNNCC 3 cut(s) 89, 290, 336
BspMI ACCTGC 3 cut(s) 205, 1058, 1091
BspPI GGATC 2 cut(s) 231, 883
BsrDI GCAATG 1 cut(s) 774
BsrI ACTGG 5 cut(s) 242, 549, 623, 661, 674
BssECI CCNNGG 3 cut(s) 124, 204, 292
BssMI GATC 6 cut(s) 223, 799, 825, 829, 888, 1071
BssNI GRCGYC 1 cut(s) 39
Bst2UI CCWGG 2 cut(s) 293, 386
Bst4CI ACNGT 1 cut(s) 583
Bst6I CTCTTC 1 cut(s) 816
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 3 cut(s) 154, 419, 631
BstDEI CTNAG 2 cut(s) 320, 507
BstDSI CCRYGG 2 cut(s) 124, 204
BstF5I GGATG 4 cut(s) 115, 171, 703, 970
BstFNI CGCG 7 cut(s) 71, 150, 152, 206, 250, 374, 432
BstHHI GCGC 3 cut(s) 131, 152, 432
BstKTI GATC 6 cut(s) 226, 802, 828, 832, 891, 1074
BstMAI GTCTC 2 cut(s) 88, 767
BstMBI GATC 6 cut(s) 223, 799, 825, 829, 888, 1071
BstMCI CGRYCG 3 cut(s) 110, 254, 832
BstMWI GCNNNNNNNGC 7 cut(s) 156, 178, 343, 427, 452, 467, 1092
BstNI CCWGG 2 cut(s) 293, 386
BstSCI CCNGG 2 cut(s) 291, 384
BstUI CGCG 7 cut(s) 71, 150, 152, 206, 250, 374, 432
BstV1I GCAGC 3 cut(s) 143, 159, 168
BstX2I RGATCY 1 cut(s) 888
BstYI RGATCY 1 cut(s) 888
BstZI CGGCCG 2 cut(s) 107, 251
Bsu15I ATCGAT 1 cut(s) 828
BsuRI GGCC 5 cut(s) 60, 109, 203, 242, 253
BsuTUI ATCGAT 1 cut(s) 828
BtgI CCRYGG 2 cut(s) 124, 204
BtsCI GGATG 4 cut(s) 115, 171, 703, 970
BtsI GCAGTG 2 cut(s) 540, 1042
BtsIMutI CAGTG 4 cut(s) 386, 540, 556, 1042
BveI ACCTGC 3 cut(s) 205, 1058, 1091
Cac8I GCNNGC 3 cut(s) 154, 419, 631
CaiI CAGNNNCTG 1 cut(s) 385
CfoI GCGC 3 cut(s) 131, 152, 432
Cfr13I GGNCC 2 cut(s) 240, 268
Cfr42I CCGCGG 1 cut(s) 207
ClaI ATCGAT 1 cut(s) 828
CseI GACGC 2 cut(s) 47, 156
Csp6I GTAC 2 cut(s) 370, 844
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviQI GTAC 2 cut(s) 370, 844
DdeI CTNAG 2 cut(s) 320, 507
DpnI GATC 6 cut(s) 225, 801, 827, 831, 890, 1073
DpnII GATC 6 cut(s) 223, 799, 825, 829, 888, 1071
DrdI GACNNNNNNGTC 2 cut(s) 80, 102
DriI GACNNNNNGTC 2 cut(s) 43, 302
DseDI GACNNNNNNGTC 2 cut(s) 80, 102
EaeI YGGCCR 2 cut(s) 107, 251
EagI CGGCCG 2 cut(s) 107, 251
Eam1104I CTCTTC 1 cut(s) 816
Eam1105I GACNNNNNGTC 2 cut(s) 43, 302
EarI CTCTTC 1 cut(s) 816
EciI GGCGGA 2 cut(s) 224, 470
Ecl136II GAGCTC 1 cut(s) 9
EclXI CGGCCG 2 cut(s) 107, 251
Eco24I GRGCYC 2 cut(s) 11, 291
Eco47I GGWCC 1 cut(s) 268
Eco52I CGGCCG 2 cut(s) 107, 251
Eco53kI GAGCTC 1 cut(s) 9
EcoICRI GAGCTC 1 cut(s) 9
EcoRII CCWGG 2 cut(s) 291, 384
EcoT38I GRGCYC 2 cut(s) 11, 291
FaiI YATR 9 cut(s) 101, 393, 395, 516, 667, 676, 789, 1055, 1144
FaqI GGGAC 1 cut(s) 309
FauI CCCGC 2 cut(s) 359, 442
FauNDI CATATG 1 cut(s) 393
FokI GGATG 4 cut(s) 102, 178, 710, 977
FriOI GRGCYC 2 cut(s) 11, 291
GlaI GCGC 3 cut(s) 130, 151, 431
GsaI CCCAGC 1 cut(s) 288
HaeIII GGCC 5 cut(s) 60, 109, 203, 242, 253
HapII CCGG 3 cut(s) 85, 91, 163
HgaI GACGC 2 cut(s) 47, 156
HhaI GCGC 3 cut(s) 131, 152, 432
Hin1I GRCGYC 1 cut(s) 39
Hin6I GCGC 3 cut(s) 129, 150, 430
HinP1I GCGC 3 cut(s) 129, 150, 430
HinfI GANTC 6 cut(s) 96, 192, 569, 604, 653, 896
HpaII CCGG 3 cut(s) 85, 91, 163
HphI GGTGA 4 cut(s) 634, 647, 896, 1025
Hpy166II GTNNAC 1 cut(s) 1019
Hpy188I TCNGA 1 cut(s) 37
Hpy188III TCNNGA 2 cut(s) 85, 803
Hpy8I GTNNAC 1 cut(s) 1019
Hpy99I CGWCG 6 cut(s) 41, 49, 108, 150, 187, 308
HpyAV CCTTC 2 cut(s) 348, 800
HpyCH4III ACNGT 1 cut(s) 583
HpyCH4IV ACGT 1 cut(s) 103
HpyCH4V TGCA 9 cut(s) 391, 417, 557, 731, 771, 978, 1047, 1053, 1095
HpyF10VI GCNNNNNNNGC 7 cut(s) 156, 178, 343, 427, 452, 467, 1092
HpyF3I CTNAG 2 cut(s) 320, 507
HpySE526I ACGT 1 cut(s) 103
Hsp92I GRCGYC 1 cut(s) 39
HspAI GCGC 3 cut(s) 129, 150, 430
Kpn2I TCCGGA 1 cut(s) 84
KspI CCGCGG 1 cut(s) 207
Kzo9I GATC 6 cut(s) 223, 799, 825, 829, 888, 1071
LmnI GCTCC 5 cut(s) 87, 211, 294, 334, 809
Lsp1109I GCAGC 3 cut(s) 143, 159, 168
LweI GCATC 7 cut(s) 124, 159, 493, 740, 780, 955, 987
MaeII ACGT 1 cut(s) 103
MaeIII GTNAC 4 cut(s) 622, 793, 1031, 1065
MalI GATC 6 cut(s) 225, 801, 827, 831, 890, 1073
MboI GATC 6 cut(s) 223, 799, 825, 829, 888, 1071
MboII GAAGA 2 cut(s) 833, 1010
MflI RGATCY 1 cut(s) 888
MhlI GDGCHC 3 cut(s) 11, 291, 533
MluCI AATT 3 cut(s) 562, 640, 1005
MluI ACGCGT 1 cut(s) 372
MlyI GAGTC 3 cut(s) 90, 201, 578
MmeI TCCRAC 1 cut(s) 60
MroI TCCGGA 1 cut(s) 84
MroXI GAANNNNTTC 1 cut(s) 948
MseI TTAA 1 cut(s) 879
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 3 cut(s) 85, 91, 163
MspR9I CCNGG 2 cut(s) 293, 386
Mva1269I GAATGC 1 cut(s) 926
MvaI CCWGG 2 cut(s) 293, 386
MvnI CGCG 7 cut(s) 71, 150, 152, 206, 250, 374, 432
MwoI GCNNNNNNNGC 7 cut(s) 156, 178, 343, 427, 452, 467, 1092
NdeI CATATG 1 cut(s) 393
NdeII GATC 6 cut(s) 223, 799, 825, 829, 888, 1071
NlaIV GGNNCC 3 cut(s) 89, 290, 336
NmuCI GTSAC 3 cut(s) 622, 793, 1031
PaqCI CACCTGC 3 cut(s) 205, 1058, 1091
PcsI WCGNNNNNNNCGW 2 cut(s) 142, 587
PctI GAATGC 1 cut(s) 926
PdmI GAANNNNTTC 1 cut(s) 948
PfeI GAWTC 3 cut(s) 604, 653, 896
Ple19I CGATCG 1 cut(s) 832
PleI GAGTC 3 cut(s) 90, 200, 577
PpsI GAGTC 3 cut(s) 90, 200, 577
Psp124BI GAGCTC 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 291, 384
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 2 cut(s) 291, 384
PspN4I GGNNCC 3 cut(s) 89, 290, 336
PspPI GGNCC 2 cut(s) 240, 268
PstNI CAGNNNCTG 1 cut(s) 385
PsuI RGATCY 1 cut(s) 888
PvuI CGATCG 1 cut(s) 832
RsaI GTAC 2 cut(s) 371, 845
RsaNI GTAC 2 cut(s) 370, 844
SacI GAGCTC 1 cut(s) 11
SacII CCGCGG 1 cut(s) 207
SaqAI TTAA 1 cut(s) 879
Sau3AI GATC 6 cut(s) 223, 799, 825, 829, 888, 1071
Sau96I GGNCC 2 cut(s) 240, 268
SchI GAGTC 3 cut(s) 90, 201, 578
ScrFI CCNGG 2 cut(s) 293, 386
SduI GDGCHC 3 cut(s) 11, 291, 533
SfaNI GCATC 7 cut(s) 124, 159, 493, 740, 780, 955, 987
Sfr303I CCGCGG 1 cut(s) 207
SgrBI CCGCGG 1 cut(s) 207
SinI GGWCC 1 cut(s) 268
Sse9I AATT 3 cut(s) 562, 640, 1005
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 2 cut(s) 291, 384
TaaI ACNGT 1 cut(s) 583
TaiI ACGT 1 cut(s) 106
TaqI TCGA 1 cut(s) 828
TasI AATT 3 cut(s) 562, 640, 1005
TatI WGTACW 1 cut(s) 843
TauI GCSGC 9 cut(s) 52, 112, 115, 162, 206, 209, 253, 446, 449
TfiI GAWTC 3 cut(s) 604, 653, 896
Tru1I TTAA 1 cut(s) 879
Tru9I TTAA 1 cut(s) 879
TscAI CASTG 4 cut(s) 386, 547, 556, 1049
TseFI GTSAC 3 cut(s) 622, 793, 1031
TseI GCWGC 3 cut(s) 131, 156, 172
Tsp45I GTSAC 3 cut(s) 622, 793, 1031
TspDTI ATGAA 1 cut(s) 471
TspGWI ACGGA 4 cut(s) 113, 185, 331, 1105
TspRI CASTG 4 cut(s) 386, 547, 556, 1049
VpaK11BI GGWCC 1 cut(s) 268
XapI RAATTY 2 cut(s) 562, 640
XcmI CCANNNNNNNNNTGG 1 cut(s) 899
XmnI GAANNNNTTC 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.