pycom05g28320

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008040
Physical Location & Seq
Reverse (-)
29088897 .. 29090744
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g28320.2

Sequence Viewer

Length: 912 bp
ATGTTGAACTCAAATCACTACCAACTCCCGTCTCCCTCCGACGCTTCGTCGCCGCTTTTAGGCCAATCGATGTCCGAAAACCTCGTCCGCAGCCGGCTGCTCATCCGTCAGCCGCCGCGCCCTCTTCGCGGCGCGGCTCGCTTCCTGCGTCGCGCCAGCAGCCGACGCATGATGCTGCGCGAGCCGTCGGTCCGGGTACGGGAGGCGGCGGCGGAGCAGCTGGAGGAGCGCCAGAGCGATTGGGCCTACTCTCGGCCGATTATCGTGTTGGACCTCTTGTGGAACGCCGGGCTCCTTGGGATTGCCGTCGGGGTGCTGTCGATGAGCTGGAAAGAGGCGCCTTCGGTGCCTTTGAGGACGTGGATTGGCGGGTACGCGTTTCAGTGCCTGGTCCATATGGTCTGCGTGGGGGTGGAGTGCAAGCGGCGGAGGCGCGGAGGTCAAGCGGCCGCGGAAGCGAGTTCCGGGTGGGAAAATTCATCTGGGTCTGGGAGTGATGATGGTGAGGACTATGGAGTTGAGCAGAGCGCCGACTACACTGCCACCAGTGTTGCAAAGCATTTGGAGTCTGCAAATACGGTGTTTTCGTTTATCTGGTGGCTTCTTGGGTTCTACTGGGTGACTGCTGGCGGTGAAAGTTTGATACGAGATTCCCCACAGTTATACTGGATTTGTATCACTTTTCTTGCTTTGGATGTTGTCTTTGTAGTCATCTGCGTTGCTGTCGTGTCTCTCATTGGTATTGCCGTTTGCTTCTGCCTCCCCTGCATCATTGCCATATTGTATGTTGTGACAGATCAGGAAGGAGCAACAAAGGAAGAGATCGATCATTTGCCAAAGTACAAGTTCCACAAGATACCTGATTTTGACAAAGTTAATGGTGATCGATCCAAGAATCTTCTGGAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

304

Amino Acids

33.73

Weight (kDa)

6.25

Isoelectric Point (pI)

62.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 337, 346
AccII CGCG 8 cut(s) 118, 129, 134, 153, 180, 377, 435, 452
AclWI GGATC 1 cut(s) 882
AcoI YGGCCR 2 cut(s) 254, 447
AcsI RAATTY 1 cut(s) 475
AcyI GRCGYC 1 cut(s) 338
AfaI GTAC 3 cut(s) 198, 374, 842
AfiI CCNNNNNNNGG 4 cut(s) 59, 128, 199, 252
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 1 cut(s) 7
AhdI GACNNNNNGTC 1 cut(s) 46
AjiI CACGTC 1 cut(s) 360
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 2 cut(s) 220, 327
AluI AGCT 2 cut(s) 220, 327
Alw26I GTCTC 2 cut(s) 36, 735
AlwI GGATC 1 cut(s) 882
AlwNI CAGNNNCTG 1 cut(s) 388
AoxI GGCC 4 cut(s) 61, 243, 254, 447
ApeKI GCWGC 5 cut(s) 90, 97, 159, 175, 217
ApoI RAATTY 1 cut(s) 475
ArsI GACNNNNNNTTYG 2 cut(s) 69, 101
AspLEI GCGC 8 cut(s) 120, 134, 155, 180, 231, 340, 435, 530
AspS9I GGNCC 4 cut(s) 190, 243, 271, 391
AsuC2I CCSGG 3 cut(s) 194, 289, 466
AsuHPI GGTGA 4 cut(s) 515, 631, 644, 893
AvaII GGWCC 3 cut(s) 190, 271, 391
BanI GGYRCC 2 cut(s) 337, 346
BanII GRGCYC 1 cut(s) 294
BbvI GCAGC 5 cut(s) 84, 102, 162, 171, 229
BccI CCATC 1 cut(s) 494
BceAI ACGGC 3 cut(s) 169, 290, 731
BcgI CGANNNNNNTGC 2 cut(s) 521, 555
BciT130I CCWGG 1 cut(s) 389
BcnI CCSGG 3 cut(s) 194, 289, 466
BcoDI GTCTC 2 cut(s) 36, 735
BfoI RGCGCY 3 cut(s) 232, 341, 531
Bme1390I CCNGG 4 cut(s) 194, 289, 389, 466
Bme18I GGWCC 3 cut(s) 190, 271, 391
BmeRI GACNNNNNGTC 1 cut(s) 46
BmgBI CACGTC 1 cut(s) 360
BmgT120I GGNCC 4 cut(s) 190, 243, 271, 391
BmiI GGNNCC 3 cut(s) 293, 339, 348
BmrFI CCNGG 4 cut(s) 194, 289, 389, 466
BmrI ACTGGG 1 cut(s) 625
BmsI GCATC 2 cut(s) 162, 777
BmuI ACTGGG 1 cut(s) 625
BpmI CTGGAG 1 cut(s) 242
BpuMI CCSGG 3 cut(s) 194, 289, 466
Bsa29I ATCGAT 3 cut(s) 68, 825, 886
BsaBI GATNNNNATC 1 cut(s) 674
BsaHI GRCGYC 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 295, 450
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 128, 199, 252
Bse118I RCCGGY 1 cut(s) 93
Bse1I ACTGG 3 cut(s) 546, 620, 671
Bse3DI GCAATG 1 cut(s) 771
Bse8I GATNNNNATC 1 cut(s) 674
BseBI CCWGG 1 cut(s) 389
BseCI ATCGAT 3 cut(s) 68, 825, 886
BseDI CCNNGG 2 cut(s) 295, 450
BseGI GGATG 2 cut(s) 102, 700
BseJI GATNNNNATC 1 cut(s) 674
BseLI CCNNNNNNNGG 4 cut(s) 59, 128, 199, 252
BseMI GCAATG 1 cut(s) 771
BseNI ACTGG 3 cut(s) 546, 620, 671
BseRI GAGGAG 1 cut(s) 239
BseX3I CGGCCG 2 cut(s) 254, 447
BseXI GCAGC 5 cut(s) 84, 102, 162, 171, 229
Bsh1236I CGCG 8 cut(s) 118, 129, 134, 153, 180, 377, 435, 452
Bsh1285I CGRYCG 2 cut(s) 257, 450
BshFI GGCC 4 cut(s) 63, 245, 256, 449
BshNI GGYRCC 2 cut(s) 337, 346
BshVI ATCGAT 3 cut(s) 68, 825, 886
BsiEI CGRYCG 2 cut(s) 257, 450
BsiSI CCGG 4 cut(s) 94, 193, 288, 465
BslI CCNNNNNNNGG 4 cut(s) 59, 128, 199, 252
BsmAI GTCTC 2 cut(s) 36, 735
BsmBI CGTCTC 1 cut(s) 36
BsnI GGCC 4 cut(s) 63, 245, 256, 449
Bsp1286I GDGCHC 1 cut(s) 294
Bsp143I GATC 5 cut(s) 796, 822, 826, 883, 887
BspANI GGCC 4 cut(s) 63, 245, 256, 449
BspDI ATCGAT 3 cut(s) 68, 825, 886
BspFNI CGCG 8 cut(s) 118, 129, 134, 153, 180, 377, 435, 452
BspLI GGNNCC 3 cut(s) 293, 339, 348
BspPI GGATC 1 cut(s) 882
BspT107I GGYRCC 2 cut(s) 337, 346
BsrDI GCAATG 1 cut(s) 771
BsrFI RCCGGY 1 cut(s) 93
BsrI ACTGG 3 cut(s) 546, 620, 671
BssAI RCCGGY 1 cut(s) 93
BssECI CCNNGG 2 cut(s) 295, 450
BssMI GATC 5 cut(s) 796, 822, 826, 883, 887
BssNI GRCGYC 1 cut(s) 338
BssT1I CCWWGG 1 cut(s) 295
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 2 cut(s) 580, 660
Bst6I CTCTTC 2 cut(s) 129, 813
BstACI GRCGYC 1 cut(s) 338
BstC8I GCNNGC 6 cut(s) 95, 139, 157, 182, 422, 628
BstDSI CCRYGG 1 cut(s) 450
BstF5I GGATG 2 cut(s) 102, 700
BstFNI CGCG 8 cut(s) 118, 129, 134, 153, 180, 377, 435, 452
BstH2I RGCGCY 3 cut(s) 232, 341, 531
BstHHI GCGC 8 cut(s) 120, 134, 155, 180, 231, 340, 435, 530
BstKTI GATC 5 cut(s) 799, 825, 829, 886, 890
BstMAI GTCTC 2 cut(s) 36, 735
BstMBI GATC 5 cut(s) 796, 822, 826, 883, 887
BstMCI CGRYCG 2 cut(s) 257, 450
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 4 cut(s) 192, 287, 387, 464
BstUI CGCG 8 cut(s) 118, 129, 134, 153, 180, 377, 435, 452
BstV1I GCAGC 5 cut(s) 84, 102, 162, 171, 229
BstZI CGGCCG 2 cut(s) 254, 447
Bsu15I ATCGAT 3 cut(s) 68, 825, 886
BsuRI GGCC 4 cut(s) 63, 245, 256, 449
BsuTUI ATCGAT 3 cut(s) 68, 825, 886
BtgI CCRYGG 1 cut(s) 450
BtrI CACGTC 1 cut(s) 360
BtsCI GGATG 2 cut(s) 102, 700
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 3 cut(s) 389, 537, 553
Cac8I GCNNGC 6 cut(s) 95, 139, 157, 182, 422, 628
CaiI CAGNNNCTG 1 cut(s) 388
CciNI GCGGCCGC 1 cut(s) 447
CfoI GCGC 8 cut(s) 120, 134, 155, 180, 231, 340, 435, 530
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 4 cut(s) 190, 243, 271, 391
Cfr42I CCGCGG 1 cut(s) 453
ClaI ATCGAT 3 cut(s) 68, 825, 886
CpoI CGGWCCG 1 cut(s) 190
CseI GACGC 3 cut(s) 50, 137, 174
Csp6I GTAC 3 cut(s) 197, 373, 841
CspCI CAANNNNNGTGG 2 cut(s) 532, 567
CspI CGGWCCG 1 cut(s) 190
CviAII CATG 1 cut(s) 169
CviQI GTAC 3 cut(s) 197, 373, 841
DinI GGCGCC 1 cut(s) 339
DpnI GATC 5 cut(s) 798, 824, 828, 885, 889
DpnII GATC 5 cut(s) 796, 822, 826, 883, 887
DriI GACNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 2 cut(s) 254, 447
EagI CGGCCG 2 cut(s) 254, 447
Eam1104I CTCTTC 2 cut(s) 129, 813
Eam1105I GACNNNNNGTC 1 cut(s) 46
EarI CTCTTC 2 cut(s) 129, 813
EciI GGCGGA 2 cut(s) 227, 442
EclXI CGGCCG 2 cut(s) 254, 447
Eco130I CCWWGG 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 294
Eco47I GGWCC 3 cut(s) 190, 271, 391
Eco52I CGGCCG 2 cut(s) 254, 447
EcoRII CCWGG 1 cut(s) 387
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 294
EgeI GGCGCC 1 cut(s) 339
EheI GGCGCC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 295
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 1 cut(s) 172
FaiI YATR 7 cut(s) 170, 396, 398, 513, 664, 779, 786
FatI CATG 1 cut(s) 168
FauI CCCGC 1 cut(s) 362
FauNDI CATATG 1 cut(s) 396
FokI GGATG 2 cut(s) 89, 707
FriOI GRGCYC 1 cut(s) 294
GlaI GCGC 8 cut(s) 119, 133, 154, 179, 230, 339, 434, 529
GsuI CTGGAG 1 cut(s) 242
HaeII RGCGCY 3 cut(s) 232, 341, 531
HaeIII GGCC 4 cut(s) 63, 245, 256, 449
HapII CCGG 4 cut(s) 94, 193, 288, 465
HgaI GACGC 3 cut(s) 50, 137, 174
HhaI GCGC 8 cut(s) 120, 134, 155, 180, 231, 340, 435, 530
Hin1I GRCGYC 1 cut(s) 338
Hin1II CATG 1 cut(s) 172
Hin6I GCGC 8 cut(s) 118, 132, 153, 178, 229, 338, 433, 528
HinP1I GCGC 8 cut(s) 118, 132, 153, 178, 229, 338, 433, 528
HinfI GANTC 3 cut(s) 566, 650, 895
HpaII CCGG 4 cut(s) 94, 193, 288, 465
HphI GGTGA 4 cut(s) 515, 631, 644, 893
Hpy188I TCNGA 2 cut(s) 40, 76
Hpy188III TCNNGA 2 cut(s) 800, 902
Hpy99I CGWCG 6 cut(s) 44, 52, 153, 168, 190, 311
HpyAV CCTTC 2 cut(s) 351, 797
HpyCH4III ACNGT 2 cut(s) 580, 660
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 4 cut(s) 420, 554, 572, 768
HpySE526I ACGT 1 cut(s) 359
Hsp92I GRCGYC 1 cut(s) 338
Hsp92II CATG 1 cut(s) 172
HspAI GCGC 8 cut(s) 118, 132, 153, 178, 229, 338, 433, 528
KasI GGCGCC 1 cut(s) 337
KroI GCCGGC 1 cut(s) 93
KroNI GCCGGC 1 cut(s) 95
KspI CCGCGG 1 cut(s) 453
Kzo9I GATC 5 cut(s) 796, 822, 826, 883, 887
LmnI GCTCC 4 cut(s) 214, 226, 297, 806
Lsp1109I GCAGC 5 cut(s) 84, 102, 162, 171, 229
LweI GCATC 2 cut(s) 162, 777
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 2 cut(s) 619, 790
MalI GATC 5 cut(s) 798, 824, 828, 885, 889
MboI GATC 5 cut(s) 796, 822, 826, 883, 887
MboII GAAGA 3 cut(s) 116, 830, 890
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 1 cut(s) 475
MluI ACGCGT 1 cut(s) 375
Mly113I GGCGCC 1 cut(s) 338
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 2 cut(s) 63, 249
MroNI GCCGGC 1 cut(s) 93
MseI TTAA 1 cut(s) 876
MspA1I CMGCKG 2 cut(s) 220, 452
MspI CCGG 4 cut(s) 94, 193, 288, 465
MspR9I CCNGG 4 cut(s) 194, 289, 389, 466
MvaI CCWGG 1 cut(s) 389
MvnI CGCG 8 cut(s) 118, 129, 134, 153, 180, 377, 435, 452
NaeI GCCGGC 1 cut(s) 95
NarI GGCGCC 1 cut(s) 338
NciI CCSGG 3 cut(s) 194, 289, 466
NdeI CATATG 1 cut(s) 396
NdeII GATC 5 cut(s) 796, 822, 826, 883, 887
NgoMIV GCCGGC 1 cut(s) 93
NlaIII CATG 1 cut(s) 172
NlaIV GGNNCC 3 cut(s) 293, 339, 348
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 2 cut(s) 619, 790
NotI GCGGCCGC 1 cut(s) 447
PcsI WCGNNNNNNNCGW 2 cut(s) 145, 584
PdiI GCCGGC 1 cut(s) 95
PfeI GAWTC 2 cut(s) 650, 895
PleI GAGTC 1 cut(s) 574
PluTI GGCGCC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 574
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 3 cut(s) 293, 339, 348
PspPI GGNCC 4 cut(s) 190, 243, 271, 391
PstNI CAGNNNCTG 1 cut(s) 388
PvuII CAGCTG 1 cut(s) 220
RsaI GTAC 3 cut(s) 198, 374, 842
RsaNI GTAC 3 cut(s) 197, 373, 841
Rsr2I CGGWCCG 1 cut(s) 190
RsrII CGGWCCG 1 cut(s) 190
SacII CCGCGG 1 cut(s) 453
SaqAI TTAA 1 cut(s) 876
Sau3AI GATC 5 cut(s) 796, 822, 826, 883, 887
Sau96I GGNCC 4 cut(s) 190, 243, 271, 391
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 4 cut(s) 194, 289, 389, 466
SduI GDGCHC 1 cut(s) 294
SetI ASST 7 cut(s) 84, 222, 276, 329, 362, 442, 862
SfaNI GCATC 2 cut(s) 162, 777
SfoI GGCGCC 1 cut(s) 339
Sfr303I CCGCGG 1 cut(s) 453
SgrBI CCGCGG 1 cut(s) 453
SinI GGWCC 3 cut(s) 190, 271, 391
Sse9I AATT 1 cut(s) 475
SspDI GGCGCC 1 cut(s) 337
StyD4I CCNGG 4 cut(s) 192, 287, 387, 464
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 2 cut(s) 580, 660
TaiI ACGT 1 cut(s) 362
TaqI TCGA 4 cut(s) 68, 320, 825, 886
TaqII GACCGA 1 cut(s) 178
TasI AATT 1 cut(s) 475
TatI WGTACW 1 cut(s) 840
TfiI GAWTC 2 cut(s) 650, 895
Tru1I TTAA 1 cut(s) 876
Tru9I TTAA 1 cut(s) 876
TscAI CASTG 3 cut(s) 389, 544, 553
TseFI GTSAC 2 cut(s) 619, 790
TseI GCWGC 5 cut(s) 90, 97, 159, 175, 217
Tsp45I GTSAC 2 cut(s) 619, 790
TspDTI ATGAA 1 cut(s) 468
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 3 cut(s) 389, 544, 553
VpaK11BI GGWCC 3 cut(s) 190, 271, 391
XapI RAATTY 1 cut(s) 475
XcmI CCANNNNNNNNNTGG 2 cut(s) 663, 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.