Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006113

Physical Location & Seq

Genomic Coordinates Forward (+)

3426172 .. 3428151

Gene Structure 1980 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1028700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1980 bp

ATGGCGGCCGTTGCTCCACCCTCCTCTTCCTCCTTCTCAAAACCCTCCCCTTTCTCTTCCTCAGCCAAATCCCACCACCCCATTTCCAGATTCAACCTCCCCTTCTCTCCTCTTCCCCAAAAACCCTCTCCCCTCCGCCACCTCCGGATCTCCAGCTCCCTATCCCCCAAAACCGATACCACAATCACCACCACCACCAGCAACCCTCTAGACCTAGAGGCCCCTATCTCCCGATTTGCCCCTGACGAGCCCAGAAAAGGCGCCGATGTCCTCGTTGAGGCACTTGAGCGCCAAGGCGTCACCGACGTCTTTGCCTACCCCGGCGGCGCATCCATGGAGATCCACCAGGCCCTCACGCGCTCCTCTTCCATCCGTAACGTTCTCCCGCGCCACGAGCAAGGCGGGGTGTTCGCGGCTGAAGGCTACGCCCGCGCGTCGGGTCGTCCCGGCGTGTGCATTGCAACCTCCGGCCCCGGCGCAACGAACTTGGTTAGCGGCCTCGCCGACGCGCTCATGGACAGCATACCGGTGGTGGCAATCACCGGCCAAGTACCCCGGAGAATGATAGGCACTGACGCCTTCCAGGAGACCCCAATTGTTGAGGTAACAAGGTCAATCACTAAGCACAATTATCTTGTTCTTGATGTAGAGGACATTCCTAGGGTTGTTAGGGAGGCATTTTTCTTAGCTACGTCGGGTCGACCTGGCCCGGTTTTGATTGATATACCCAAAGATGTGCAACAACAGCTTGTGGTTCCCAATTGGGATCAACCCATTAAGTTACCCGGGTACATGTCTAGGTTGCCCAAGTCCCCCAATGAGGGTCACTTGGAGCAGATTGTGAGGTTGGTGTCTGAGTCCAAGAAACCGGTTTTGTATGTTGGTGGAGGGTGTTTGAACTCCAGTGAGGAGTTGAGGCGGTTCGTGGAGCTCACCGGGATCCCCGTGGCTAGTACTTTGATGGGTCTCGGGGCATACCCGTGCAATGACGAAGAATTGTCACTCCAAATGCTTGGAATGCATGGCACTGTTTATGCTAATTATGCTGTGGATAAGTCTGATCTGTTGCTTGCATTTGGGGTGAGGTTTGATGACCGGGTTACTGGGAAGTTGGAGGCATTTGCTAGCCGGGCTAAGATTGTTCACATTGATATTGACTCGGCGGAGATTGGAAAGAATAAGCAGCCTCATGTGTCGGTTTGTGCTGATGTAAAATCGGCATTGGAAGGGTTGAATAGGATATTGGAGCAGAAAGAGAGCAAGGTTAAGCTTGATTTTTCGGCTTGGAGGGCGGAGCTGAAAGAGGAGAAAGTGAAGTTTCCATTGGGTTTTAAGACTTTTGGGGACTCCATTTCTCCTCAGAATGCCATTCAGGTTCTTGATGAGTTAACTGATGGGAACGCGATTATAAGCACTGGTGTTGGACAGCATCAAATGTGGGCAGCTCAGTTTTACAAGTACAAGCGGCCTCGGCAGTGGTTGACATCAGGGGGATTAGGAGCTATGGGCTTCGGATTGCCTGCTGCTATCGGGGCTGCTGTTGCAAACCCGGATTCTGTTGTCGTTGACATTGATGGTGATGGAAGTTTCCTTATGAATGTCCAGGAATTGGCAACGATCAGTGTGGAGAAACTTCCGGTCAAGATAATGCTGTTGAATAACCAACATTTGGGTATGGTTGTGCAATGGGAGGATCGCTTCTACAAGGCAAACAGAGCTCATACGTACTTGGGGAACCCAGCAAATGAGTCGGAGATATTCCCAAATATGCTCAAGTTTGCAGATGCTTGTGGGATACCAGCTGCCCGTGTGACGAAGAAGGAAGATCTGAAAGCAGCAATTCAGAAAATGTTGGACACTCCTGGTCCGTACTTGTTGGATGTAATAGTGCCCCATCAAGAGCATGTCCTGCCTATGATTCCGAGCGGCGGTGCTTTCAAAGATGTGATAACGGAGGGCGATGGGAGGTCGAGTTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009099 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

660

Amino Acids

71.44

Weight (kDa)

6.34

Isoelectric Point (pI)

44.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	86 - 196	9e-38	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	277 - 411	3.8e-44	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	473 - 628	6.5e-51	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48560
fragaria_vesca	FvH4_7g06770
malus_domestica	MD06G1028700.v1.1 MD10G1013700.v1.1
prunus_persica	Prupe.5G043100_v2.0.a1 Prupe.8G001300_v2.0.a1 Prupe.8G001300_v2.0.a1
pyrus_communis	pycom06g02280 pycom10g00840
rosa_chinensis	RchiOBHm_Chr7g0208791
rosa_laevigata	RLG00000003195 RLG00000015583
rosa_multiflora	Rmu_co8493263.1_g000001 Rmu_sc0000278.1_g000002 Rmu_sc0008663.1_g000001 Rmu_sc0018750.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250260 Rroxscaffold_7G00217760
rosa_rugosa	Rorug05G0492000 Rorug07G0105800
rosa_samantha	Rh6AG001800 Rh7AG241000 Rh7BG235900 Rh7CG255600 Rh7DG247000
rosa_wichuraiana	Rw6G000240 Rw7G020440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1409
AatII	GACGTC	1 cut(s)	309
AccB1I	GGYRCC	1 cut(s)	260
AccB7I	CCANNNNNTGG	2 cut(s)	1609, 1669
AccBSI	CCGCTC	1 cut(s)	1926
AccI	GTMKAC	1 cut(s)	700
AccII	CGCG	7 cut(s)	358, 388, 413, 432, 434, 509, 1403
AccIII	TCCGGA	1 cut(s)	144
AclI	AACGTT	1 cut(s)	378
AclWI	GGATC	6 cut(s)	155, 334, 774, 934, 947, 1701
AcoI	YGGCCR	2 cut(s)	6, 544
AcuI	CTGAAG	1 cut(s)	438
AcyI	GRCGYC	4 cut(s)	261, 297, 306, 576
AfaI	GTAC	6 cut(s)	552, 791, 955, 1460, 1727, 1871
AfiI	CCNNNNNNNGG	8 cut(s)	257, 277, 436, 820, 821, 1609, 1669, 1928
AflIII	ACRYGT	1 cut(s)	792
AgeI	ACCGGT	2 cut(s)	526, 868
AgsI	TTSAA	5 cut(s)	94, 898, 1234, 1657, 1939
AjnI	CCWGG	5 cut(s)	345, 582, 703, 1602, 1861
AjuI	GAANNNNNNNTTGG	4 cut(s)	1226, 1258, 1307, 1339
Alw21I	GWGCWC	2 cut(s)	933, 1720
Alw26I	GTCTC	2 cut(s)	581, 971
AlwI	GGATC	6 cut(s)	155, 334, 774, 934, 947, 1701
Ama87I	CYCGRG	2 cut(s)	785, 968
Aor13HI	TCCGGA	1 cut(s)	144
AoxI	GGCC	8 cut(s)	6, 219, 348, 469, 496, 544, 706, 1466
ApeKI	GCWGC	6 cut(s)	1183, 1442, 1523, 1535, 1802, 1835
AsiGI	ACCGGT	2 cut(s)	526, 868
AspA2I	CCTAGG	1 cut(s)	659
AspLEI	GCGC	8 cut(s)	263, 291, 329, 360, 390, 434, 479, 511
AspS9I	GGNCC	5 cut(s)	220, 349, 470, 707, 1865
AsuHPI	GGTGA	6 cut(s)	178, 292, 532, 925, 1093, 1589
AsuNHI	GCTAGC	1 cut(s)	1124
AvaI	CYCGRG	2 cut(s)	785, 968
AvaII	GGWCC	1 cut(s)	1865
AvrII	CCTAGG	1 cut(s)	659
BaeGI	GKGCMC	1 cut(s)	1893
BamHI	GGATCC	1 cut(s)	939
BanI	GGYRCC	1 cut(s)	260
BanII	GRGCYC	3 cut(s)	252, 933, 1720
BauI	CACGAG	1 cut(s)	392
Bbv12I	GWGCWC	2 cut(s)	933, 1720
BbvCI	CCTCAGC	1 cut(s)	61
BbvI	GCAGC	6 cut(s)	1195, 1454, 1510, 1522, 1789, 1847
BccI	CCATC	7 cut(s)	377, 955, 1388, 1568, 1574, 1902, 1955
BcgI	CGANNNNNNTGC	4 cut(s)	293, 327, 1731, 1765
BciT130I	CCWGG	5 cut(s)	347, 584, 705, 1604, 1863
BciVI	GTATCC	1 cut(s)	1788
BcoDI	GTCTC	2 cut(s)	581, 971
BfaI	CTAG	6 cut(s)	209, 215, 660, 798, 951, 1125
BfoI	RGCGCY	2 cut(s)	264, 292
BfuI	GTATCC	1 cut(s)	1788
BglII	AGATCT	1 cut(s)	1825
BlnI	CCTAGG	1 cut(s)	659
BmcAI	AGTACT	1 cut(s)	955
Bme18I	GGWCC	1 cut(s)	1865
BmeT110I	CYCGRG	2 cut(s)	785, 968
BmgT120I	GGNCC	5 cut(s)	220, 349, 470, 707, 1865
BmiI	GGNNCC	6 cut(s)	222, 262, 472, 756, 941, 1736
BmrI	ACTGGG	1 cut(s)	1113
BmsI	GCATC	3 cut(s)	338, 1438, 1774
BmtI	GCTAGC	1 cut(s)	1128
BmuI	ACTGGG	1 cut(s)	1113
BpmI	CTGGAG	2 cut(s)	136, 886
Bpu10I	CCTNAGC	1 cut(s)	61
BpuEI	CTTGAG	2 cut(s)	305, 1757
BsaAI	YACGTR	1 cut(s)	1725
BsaHI	GRCGYC	4 cut(s)	261, 297, 306, 576
BsaI	GGTCTC	2 cut(s)	581, 971
BsaJI	CCNNGG	9 cut(s)	292, 319, 333, 472, 554, 659, 785, 945, 1469
BsaWI	WCCGGW	4 cut(s)	144, 526, 868, 1636
BsaXI	ACNNNNNCTCC	1 cut(s)	1957
Bsc4I	CCNNNNNNNGG	8 cut(s)	257, 277, 436, 820, 821, 1609, 1669, 1928
Bse118I	RCCGGY	3 cut(s)	526, 542, 868
Bse1I	ACTGG	3 cut(s)	903, 1108, 1420
Bse3DI	GCAATG	3 cut(s)	456, 991, 1691
BseAI	TCCGGA	1 cut(s)	144
BseBI	CCWGG	5 cut(s)	347, 584, 705, 1604, 1863
BseDI	CCNNGG	9 cut(s)	292, 319, 333, 472, 554, 659, 785, 945, 1469
BseGI	GGATG	3 cut(s)	329, 369, 1885
BseLI	CCNNNNNNNGG	8 cut(s)	257, 277, 436, 820, 821, 1609, 1669, 1928
BseMI	GCAATG	3 cut(s)	456, 991, 1691
BseMII	CTCAG	4 cut(s)	75, 846, 1373, 1460
BseNI	ACTGG	3 cut(s)	903, 1108, 1420
BseRI	GAGGAG	6 cut(s)	13, 99, 352, 923, 1319, 1347
BseSI	GKGCMC	1 cut(s)	1893
BseX3I	CGGCCG	1 cut(s)	6
BseXI	GCAGC	6 cut(s)	1195, 1454, 1510, 1522, 1789, 1847
BseYI	CCCAGC	1 cut(s)	1738
Bsh1236I	CGCG	7 cut(s)	358, 388, 413, 432, 434, 509, 1403
Bsh1285I	CGRYCG	1 cut(s)	9
BshFI	GGCC	8 cut(s)	8, 221, 350, 471, 498, 546, 708, 1468
BshNI	GGYRCC	1 cut(s)	260
BshTI	ACCGGT	2 cut(s)	526, 868
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	2 cut(s)	933, 1720
BsiHKCI	CYCGRG	2 cut(s)	785, 968
BslFI	GGGAC	3 cut(s)	429, 796, 1358
BslI	CCNNNNNNNGG	8 cut(s)	257, 277, 436, 820, 821, 1609, 1669, 1928
BsmAI	GTCTC	2 cut(s)	581, 971
BsmFI	GGGAC	3 cut(s)	429, 796, 1358
BsmI	GAATGC	2 cut(s)	1023, 1369
BsnI	GGCC	8 cut(s)	8, 221, 350, 471, 498, 546, 708, 1468
Bso31I	GGTCTC	2 cut(s)	581, 971
BsoBI	CYCGRG	2 cut(s)	785, 968
Bsp1286I	GDGCHC	4 cut(s)	252, 933, 1720, 1893
Bsp13I	TCCGGA	1 cut(s)	144
Bsp143I	GATC	8 cut(s)	147, 339, 766, 939, 1060, 1617, 1693, 1825
Bsp19I	CCATGG	1 cut(s)	333
BspANI	GGCC	8 cut(s)	8, 221, 350, 471, 498, 546, 708, 1468
BspCNI	CTCAG	4 cut(s)	74, 847, 1372, 1459
BspEI	TCCGGA	1 cut(s)	144
BspFNI	CGCG	7 cut(s)	358, 388, 413, 432, 434, 509, 1403
BspLI	GGNNCC	6 cut(s)	222, 262, 472, 756, 941, 1736
BspOI	GCTAGC	1 cut(s)	1128
BspPI	GGATC	6 cut(s)	155, 334, 774, 934, 947, 1701
BspT107I	GGYRCC	1 cut(s)	260
BspTNI	GGTCTC	2 cut(s)	581, 971
BsrBI	CCGCTC	1 cut(s)	1926
BsrDI	GCAATG	3 cut(s)	456, 991, 1691
BsrFI	RCCGGY	3 cut(s)	526, 542, 868
BsrI	ACTGG	3 cut(s)	903, 1108, 1420
BssAI	RCCGGY	3 cut(s)	526, 542, 868
BssECI	CCNNGG	9 cut(s)	292, 319, 333, 472, 554, 659, 785, 945, 1469
BssMI	GATC	8 cut(s)	147, 339, 766, 939, 1060, 1617, 1693, 1825
BssNI	GRCGYC	4 cut(s)	261, 297, 306, 576
BssSI	CACGAG	1 cut(s)	392
BssT1I	CCWWGG	3 cut(s)	292, 333, 659
Bst2BI	CACGAG	1 cut(s)	392
Bst2UI	CCWGG	5 cut(s)	347, 584, 705, 1604, 1863
Bst4CI	ACNGT	1 cut(s)	1030
Bst6I	CTCTTC	4 cut(s)	31, 61, 117, 370
BstACI	GRCGYC	4 cut(s)	261, 297, 306, 576
BstAPI	GCANNNNNTGC	1 cut(s)	1909
BstBAI	YACGTR	1 cut(s)	1725
BstC8I	GCNNGC	4 cut(s)	430, 1071, 1126, 1521
BstDEI	CTNAG	7 cut(s)	61, 621, 685, 855, 1134, 1359, 1446
BstDSI	CCRYGG	2 cut(s)	333, 945
BstENI	CCTNNNNNAGG	1 cut(s)	275
BstF5I	GGATG	3 cut(s)	329, 369, 1885
BstFNI	CGCG	7 cut(s)	358, 388, 413, 432, 434, 509, 1403
BstH2I	RGCGCY	2 cut(s)	264, 292
BstHHI	GCGC	8 cut(s)	263, 291, 329, 360, 390, 434, 479, 511
BstKTI	GATC	8 cut(s)	150, 342, 769, 942, 1063, 1620, 1696, 1828
BstMAI	GTCTC	2 cut(s)	581, 971
BstMBI	GATC	8 cut(s)	147, 339, 766, 939, 1060, 1617, 1693, 1825
BstMCI	CGRYCG	1 cut(s)	9
BstNI	CCWGG	5 cut(s)	347, 584, 705, 1604, 1863
BstNSI	RCATGY	2 cut(s)	796, 1907
BstSLI	GKGCMC	1 cut(s)	1893
BstSNI	TACGTA	1 cut(s)	1725
BstUI	CGCG	7 cut(s)	358, 388, 413, 432, 434, 509, 1403
BstV1I	GCAGC	6 cut(s)	1195, 1454, 1510, 1522, 1789, 1847
BstX2I	RGATCY	4 cut(s)	147, 339, 939, 1825
BstXI	CCANNNNNNTGG	1 cut(s)	1013
BstYI	RGATCY	4 cut(s)	147, 339, 939, 1825
BstZI	CGGCCG	1 cut(s)	6
BsuI	GTATCC	1 cut(s)	1788
BsuRI	GGCC	8 cut(s)	8, 221, 350, 471, 498, 546, 708, 1468
BtgI	CCRYGG	2 cut(s)	333, 945
BtgZI	GCGATG	1 cut(s)	1974
BtsCI	GGATG	3 cut(s)	329, 369, 1885
BtsI	GCAGTG	1 cut(s)	1481
BtsIMutI	CAGTG	6 cut(s)	570, 910, 1026, 1413, 1481, 1627
Cac8I	GCNNGC	4 cut(s)	430, 1071, 1126, 1521
CfoI	GCGC	8 cut(s)	263, 291, 329, 360, 390, 434, 479, 511
Cfr10I	RCCGGY	3 cut(s)	526, 542, 868
Cfr13I	GGNCC	5 cut(s)	220, 349, 470, 707, 1865
Cfr9I	CCCGGG	1 cut(s)	785
CseI	GACGC	4 cut(s)	286, 423, 515, 584
Csp6I	GTAC	6 cut(s)	551, 790, 954, 1459, 1726, 1870
CspAI	ACCGGT	2 cut(s)	526, 868
CviAII	CATG	6 cut(s)	334, 514, 793, 1022, 1190, 1904
CviQI	GTAC	6 cut(s)	551, 790, 954, 1459, 1726, 1870
DdeI	CTNAG	7 cut(s)	61, 621, 685, 855, 1134, 1359, 1446
DinI	GGCGCC	1 cut(s)	262
DpnI	GATC	8 cut(s)	149, 341, 768, 941, 1062, 1619, 1695, 1827
DpnII	GATC	8 cut(s)	147, 339, 766, 939, 1060, 1617, 1693, 1825
EaeI	YGGCCR	2 cut(s)	6, 544
EagI	CGGCCG	1 cut(s)	6
Eam1104I	CTCTTC	4 cut(s)	31, 61, 117, 370
EarI	CTCTTC	4 cut(s)	31, 61, 117, 370
EciI	GGCGGA	3 cut(s)	125, 1178, 1307
Ecl136II	GAGCTC	2 cut(s)	931, 1718
EclXI	CGGCCG	1 cut(s)	6
Eco105I	TACGTA	1 cut(s)	1725
Eco130I	CCWWGG	3 cut(s)	292, 333, 659
Eco24I	GRGCYC	3 cut(s)	252, 933, 1720
Eco31I	GGTCTC	2 cut(s)	581, 971
Eco47I	GGWCC	1 cut(s)	1865
Eco52I	CGGCCG	1 cut(s)	6
Eco53kI	GAGCTC	2 cut(s)	931, 1718
Eco57I	CTGAAG	1 cut(s)	438
Eco88I	CYCGRG	2 cut(s)	785, 968
EcoICRI	GAGCTC	2 cut(s)	931, 1718
EcoNI	CCTNNNNNAGG	1 cut(s)	275
EcoO109I	RGGNCCY	2 cut(s)	220, 349
EcoRII	CCWGG	5 cut(s)	345, 582, 703, 1602, 1861
EcoT14I	CCWWGG	3 cut(s)	292, 333, 659
EcoT22I	ATGCAT	1 cut(s)	1023
EcoT38I	GRGCYC	3 cut(s)	252, 933, 1720
EgeI	GGCGCC	1 cut(s)	262
EheI	GGCGCC	1 cut(s)	262
ErhI	CCWWGG	3 cut(s)	292, 333, 659
FaeI	CATG	6 cut(s)	337, 517, 796, 1025, 1193, 1907
FaqI	GGGAC	3 cut(s)	429, 796, 1358
FatI	CATG	6 cut(s)	333, 513, 792, 1021, 1189, 1903
FauI	CCCGC	3 cut(s)	393, 395, 437
FblI	GTMKAC	1 cut(s)	700
FokI	GGATG	3 cut(s)	316, 356, 1892
FriOI	GRGCYC	3 cut(s)	252, 933, 1720
FspBI	CTAG	6 cut(s)	209, 215, 660, 798, 951, 1125
GlaI	GCGC	8 cut(s)	262, 290, 328, 359, 389, 433, 478, 510
GsaI	CCCAGC	1 cut(s)	1742
GsuI	CTGGAG	2 cut(s)	136, 886
HaeII	RGCGCY	2 cut(s)	264, 292
HaeIII	GGCC	8 cut(s)	8, 221, 350, 471, 498, 546, 708, 1468
HgaI	GACGC	4 cut(s)	286, 423, 515, 584
HhaI	GCGC	8 cut(s)	263, 291, 329, 360, 390, 434, 479, 511
Hin1I	GRCGYC	4 cut(s)	261, 297, 306, 576
Hin1II	CATG	6 cut(s)	337, 517, 796, 1025, 1193, 1907
Hin6I	GCGC	8 cut(s)	261, 289, 327, 358, 388, 432, 477, 509
HinP1I	GCGC	8 cut(s)	261, 289, 327, 358, 388, 432, 477, 509
HincII	GTYRAC	4 cut(s)	701, 1389, 1482, 1567
HindII	GTYRAC	4 cut(s)	701, 1389, 1482, 1567
HindIII	AAGCTT	1 cut(s)	1268
HinfI	GANTC	7 cut(s)	90, 857, 1157, 1346, 1553, 1748, 1918
HpaI	GTTAAC	1 cut(s)	1389
HphI	GGTGA	6 cut(s)	178, 292, 532, 925, 1093, 1589
Hpy166II	GTNNAC	5 cut(s)	701, 1144, 1389, 1482, 1567
Hpy188I	TCNGA	8 cut(s)	856, 1060, 1362, 1514, 1753, 1830, 1845, 1923
Hpy188III	TCNNGA	8 cut(s)	87, 145, 209, 231, 641, 1379, 1642, 1898
Hpy8I	GTNNAC	5 cut(s)	701, 1144, 1389, 1482, 1567
Hpy99I	CGWCG	4 cut(s)	308, 439, 509, 697
HpyAV	CCTTC	6 cut(s)	43, 112, 413, 589, 1220, 1813
HpyCH4III	ACNGT	1 cut(s)	1030
HpyCH4IV	ACGT	4 cut(s)	306, 378, 692, 1724
HpyCH4V	TGCA	9 cut(s)	456, 461, 739, 984, 1021, 1073, 1544, 1684, 1781
HpyF3I	CTNAG	7 cut(s)	61, 621, 685, 855, 1134, 1359, 1446
HpySE526I	ACGT	4 cut(s)	306, 378, 692, 1724
Hsp92I	GRCGYC	4 cut(s)	261, 297, 306, 576
Hsp92II	CATG	6 cut(s)	337, 517, 796, 1025, 1193, 1907
HspAI	GCGC	8 cut(s)	261, 289, 327, 358, 388, 432, 477, 509
KasI	GGCGCC	1 cut(s)	260
Kpn2I	TCCGGA	1 cut(s)	144
KspAI	GTTAAC	1 cut(s)	1389
Kzo9I	GATC	8 cut(s)	147, 339, 766, 939, 1060, 1617, 1693, 1825
LmnI	GCTCC	8 cut(s)	19, 161, 365, 832, 928, 1246, 1294, 1499
Lsp1109I	GCAGC	6 cut(s)	1195, 1454, 1510, 1522, 1789, 1847
LweI	GCATC	3 cut(s)	338, 1438, 1774
MaeI	CTAG	6 cut(s)	209, 215, 660, 798, 951, 1125
MaeII	ACGT	4 cut(s)	306, 378, 692, 1724
MaeIII	GTNAC	8 cut(s)	298, 374, 604, 780, 824, 999, 1099, 1810
MalI	GATC	8 cut(s)	149, 341, 768, 941, 1062, 1619, 1695, 1827
MbiI	CCGCTC	1 cut(s)	1926
MboI	GATC	8 cut(s)	147, 339, 766, 939, 1060, 1617, 1693, 1825
MboII	GAAGA	7 cut(s)	18, 48, 104, 357, 1004, 1828, 1835
MfeI	CAATTG	2 cut(s)	594, 760
MflI	RGATCY	4 cut(s)	147, 339, 939, 1825
MhlI	GDGCHC	4 cut(s)	252, 933, 1720, 1893
MluCI	AATT	7 cut(s)	594, 628, 760, 995, 1039, 1607, 1839
Mly113I	GGCGCC	1 cut(s)	261
MlyI	GAGTC	4 cut(s)	866, 1151, 1340, 1757
MmeI	TCCRAC	5 cut(s)	1092, 1402, 1731, 1833, 1857
Mph1103I	ATGCAT	1 cut(s)	1023
MroI	TCCGGA	1 cut(s)	144
MseI	TTAA	4 cut(s)	777, 1266, 1332, 1388
MslI	CAYNNNNRTG	2 cut(s)	527, 979
MspA1I	CMGCKG	1 cut(s)	1802
MunI	CAATTG	2 cut(s)	594, 760
Mva1269I	GAATGC	2 cut(s)	1023, 1369
MvaI	CCWGG	5 cut(s)	347, 584, 705, 1604, 1863
MvnI	CGCG	7 cut(s)	358, 388, 413, 432, 434, 509, 1403
NarI	GGCGCC	1 cut(s)	261
NcoI	CCATGG	1 cut(s)	333
NdeII	GATC	8 cut(s)	147, 339, 766, 939, 1060, 1617, 1693, 1825
NheI	GCTAGC	1 cut(s)	1124
NlaIII	CATG	6 cut(s)	337, 517, 796, 1025, 1193, 1907
NlaIV	GGNNCC	6 cut(s)	222, 262, 472, 756, 941, 1736
NmeAIII	GCCGAG	2 cut(s)	1139, 1450
NmuCI	GTSAC	4 cut(s)	298, 824, 999, 1810
NsiI	ATGCAT	1 cut(s)	1023
NspI	RCATGY	2 cut(s)	796, 1907
PciI	ACATGT	1 cut(s)	792
PctI	GAATGC	2 cut(s)	1023, 1369
PfeI	GAWTC	3 cut(s)	90, 1553, 1918
PflMI	CCANNNNNTGG	2 cut(s)	1609, 1669
PfoI	TCCNGGA	2 cut(s)	582, 1602
PinAI	ACCGGT	2 cut(s)	526, 868
PleI	GAGTC	4 cut(s)	865, 1151, 1340, 1756
PluTI	GGCGCC	1 cut(s)	264
PpsI	GAGTC	4 cut(s)	865, 1151, 1340, 1756
Ppu21I	YACGTR	1 cut(s)	1725
PscI	ACATGT	1 cut(s)	792
PsiI	TTATAA	1 cut(s)	1409
Psp124BI	GAGCTC	2 cut(s)	933, 1720
Psp1406I	AACGTT	1 cut(s)	378
Psp6I	CCWGG	5 cut(s)	345, 582, 703, 1602, 1861
PspFI	CCCAGC	1 cut(s)	1738
PspGI	CCWGG	5 cut(s)	345, 582, 703, 1602, 1861
PspN4I	GGNNCC	6 cut(s)	222, 262, 472, 756, 941, 1736
PspPI	GGNCC	5 cut(s)	220, 349, 470, 707, 1865
PsuI	RGATCY	4 cut(s)	147, 339, 939, 1825
PvuII	CAGCTG	1 cut(s)	1802
RsaI	GTAC	6 cut(s)	552, 791, 955, 1460, 1727, 1871
RsaNI	GTAC	6 cut(s)	551, 790, 954, 1459, 1726, 1870
RseI	CAYNNNNRTG	2 cut(s)	527, 979
SacI	GAGCTC	2 cut(s)	933, 1720
SalI	GTCGAC	1 cut(s)	699
SaqAI	TTAA	4 cut(s)	777, 1266, 1332, 1388
Sau3AI	GATC	8 cut(s)	147, 339, 766, 939, 1060, 1617, 1693, 1825
Sau96I	GGNCC	5 cut(s)	220, 349, 470, 707, 1865
ScaI	AGTACT	1 cut(s)	955
SchI	GAGTC	4 cut(s)	866, 1151, 1340, 1757
SduI	GDGCHC	4 cut(s)	252, 933, 1720, 1893
SfaNI	GCATC	3 cut(s)	338, 1438, 1774
SfoI	GGCGCC	1 cut(s)	262
SinI	GGWCC	1 cut(s)	1865
SmaI	CCCGGG	1 cut(s)	787
SmiMI	CAYNNNNRTG	2 cut(s)	527, 979
SmlI	CTYRAG	2 cut(s)	284, 1772
SmoI	CTYRAG	2 cut(s)	284, 1772
SnaBI	TACGTA	1 cut(s)	1725
Sse9I	AATT	7 cut(s)	594, 628, 760, 995, 1039, 1607, 1839
SspDI	GGCGCC	1 cut(s)	260
SspMI	CTAG	6 cut(s)	209, 215, 660, 798, 951, 1125
SstI	GAGCTC	2 cut(s)	933, 1720
StyI	CCWWGG	3 cut(s)	292, 333, 659
TaaI	ACNGT	1 cut(s)	1030
TaiI	ACGT	4 cut(s)	309, 381, 695, 1727
TaqI	TCGA	2 cut(s)	700, 1970
TasI	AATT	7 cut(s)	594, 628, 760, 995, 1039, 1607, 1839
TatI	WGTACW	2 cut(s)	953, 1458
TauI	GCSGC	6 cut(s)	8, 327, 416, 498, 1468, 1929
TfiI	GAWTC	3 cut(s)	90, 1553, 1918
Tru1I	TTAA	4 cut(s)	777, 1266, 1332, 1388
Tru9I	TTAA	4 cut(s)	777, 1266, 1332, 1388
TscAI	CASTG	6 cut(s)	577, 910, 1033, 1420, 1481, 1627
TseFI	GTSAC	4 cut(s)	298, 824, 999, 1810
TseI	GCWGC	6 cut(s)	1183, 1442, 1523, 1535, 1802, 1835
Tsp45I	GTSAC	4 cut(s)	298, 824, 999, 1810
TspDTI	ATGAA	1 cut(s)	1610
TspGWI	ACGGA	3 cut(s)	362, 1857, 1967
TspMI	CCCGGG	1 cut(s)	785
TspRI	CASTG	6 cut(s)	577, 910, 1033, 1420, 1481, 1627
Van91I	CCANNNNNTGG	2 cut(s)	1609, 1669
VpaK11BI	GGWCC	1 cut(s)	1865
XagI	CCTNNNNNAGG	1 cut(s)	275
XbaI	TCTAGA	1 cut(s)	208
XceI	RCATGY	2 cut(s)	796, 1907
XmaI	CCCGGG	1 cut(s)	785
XmaJI	CCTAGG	1 cut(s)	659
XmiI	GTMKAC	1 cut(s)	700
XspI	CTAG	6 cut(s)	209, 215, 660, 798, 951, 1125
ZraI	GACGTC	1 cut(s)	307
ZrmI	AGTACT	1 cut(s)	955
Zsp2I	ATGCAT	1 cut(s)	1023

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.