Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0006113

Physical Location & Seq

Genomic Coordinates Forward (+)

25459952 .. 25462226

Gene Structure 2275 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG247000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGAAATATTTAAACCATAGAAAAAGGGAAATTAAAAATATAAATAGGATAAACTCTTGTGGACAAGAGCCAACCTCTTCGCAGCCACACTGGAGCAATCAAAATCTCTGCACTCTTGAGAGGCGCCTCTCTCTCACCTCGTCAACAATGGCGACCATAGCTCCACCCTCTTCCTCCTTCTCCAAACCCTCTCTTCCCACCAAATCCCCAAACCCACTTTCCCGATTTGCCCTCCCCTACTTCCCTTACCCCAACAAGCACACACCCTTCCGCCCTCTCCACATCTCCAACTCTCTCACCCCCAAAGCCACCACCACACCTGTCACCGCCACCACAACAATCACCGCTGCTCCTCCAGACCCAGCAACTCCAATTCCCCGATTTGCCCCTGACGAGCCCAGAAAAGGCGCCGATGTCCTCGTGGAGGCCCTCGAGCGCCAGGGCGTCACCACTGTCTTCGCCTACCCCGGCGGCGCGTCGATGGAGATCCACCAGGCCCTCACGCGCTCCACCACCATCCGCAACGTCCTCCCCCGCCACGAGCAGGGCGGCGTCTTCGCAGCCGAGGGCTATGCGCGCGCGTCGGGCCTTCCGGGCGTATGCATTGCCACCTCCGGCCCCGGCGCGACCAATCTGGTCAGCGGTCTTGCCGACGCGCTCATGGACAGCATTCCGATGGTGGCAATCACCGGACAAGTGCCCCGCCGGATGATCGGAACTGATGCTTTTCAGGAGACGCCGATTGTTGAGCAACAGCTTGTGGTTCCGGATTGGGACCAACCCATTAGGTTAACTGGTTACATGTCTAGGTTGCCCAAAAGCCCCAGTGAGGTACATCTGGAGCAGATTGTGAGGTTGGTGTCGGAGTCGAAGAAACCGGTGTTGTATGTTGGTGGAGGGTGTTTGAACTCGAGTGAGGAGTTGAGGCGGTTCGTGGAGCTTACTGGGATCCCAGTTACTAGTACTTTGATGGGTCTCGGGACTTACCCGAGCTCCGATGAAGATGACTTGTCACTGCAGATGCTTGGGATGCATGGAACTGTTTATGCTAACTATGCTGTGGACAAGGCTGACTTGTTGCTGGCTTTTGGTGTGAGGTTTGATGATCGTGTGACTGGGAAGCTGGAGGCATTTGCTAGCCGGGCTAAGATTGTGCACATTGATATTGATTCTGCAGAGATTGGGAAGAACAAGCAGCCTCATGTGTCTATTTGTGGTGATGTGAAACCAGCGTTGCAAGGGTTGAACAGGATATTGGAGGGGAAATTGGAGAAGCTTAAGCTTGATTTTTCAGCTTGGAGGGCGGAGCTGAAAGAGCAGAAATTGAAGTTTCCATTGGGTTACAAGACTTTTGGGGAGGCCATCCCTCCTCAGTATGCCATTCAGGTTCTTGATGAGTTAACTGAAGGGAAGGCTATTATAAGCACTGGTGTTGGGCAACATCAGATGTGGGCGGCTCAACATTACAAGTATCTTAGGCCCCGACAGTGGTTGACTTCGGGGGGTTTAGGAGCCATGGGTTTTGGATTGCCAGCTGCTATGGGGGCTGCGGCAGCAAATCCAGGTGCTATTGTTGTCGACATTGATGGTGATGGAAGTTTTATGATGAATGTCCAAGAATTGGCAACAATCAAGGTGGAGAATCTTCCTGTCAAAATTCTGCTGTTGAACAATCAACATTTGGGTATGGTTGTTCAGTGGGAGGATCGCTTCTATAAGGCAAACAGGGCTCACACATACTTGGGCAACCCAGCAAATGAGTCTGAGATCTTCCCAAATATGCTAAAGTTCGCAGAAGCTTGTGGGGTACCAGCTGCCCGTGTGACGAAGAAGGAAGACCTCAAAGCGGCAATCCAGAAAATGTTGGACACTCCTGGGCCATACTTGTTGGACGTTATTGTACCACATCAAGAGCATGTGTTGCCTATGATCCCAAGCGGTGGTGCTTTCAAAGATGTGATAACTGAGGGTGATGGAAGAAGAAGTTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009099 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

663

Amino Acids

72.27

Weight (kDa)

8.32

Isoelectric Point (pI)

44.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	135 - 246	4.5e-37	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	280 - 415	6.1e-45	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	477 - 632	3.7e-51	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48560
fragaria_vesca	FvH4_7g06770
malus_domestica	MD06G1028700.v1.1 MD10G1013700.v1.1
prunus_persica	Prupe.5G043100_v2.0.a1 Prupe.8G001300_v2.0.a1 Prupe.8G001300_v2.0.a1
pyrus_communis	pycom06g02280 pycom10g00840
rosa_chinensis	RchiOBHm_Chr7g0208791
rosa_laevigata	RLG00000003195 RLG00000015583
rosa_multiflora	Rmu_co8493263.1_g000001 Rmu_sc0000278.1_g000002 Rmu_sc0008663.1_g000001 Rmu_sc0018750.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250260 Rroxscaffold_7G00217760
rosa_rugosa	Rorug05G0492000 Rorug07G0105800
rosa_samantha	Rh6AG001800 Rh7AG241000 Rh7BG235900 Rh7CG255600 Rh7DG247000
rosa_wichuraiana	Rw6G000240 Rw7G020440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1421
Acc65I	GGTACC	1 cut(s)	1807
AccB1I	GGYRCC	3 cut(s)	123, 407, 1807
AccB7I	CCANNNNNTGG	2 cut(s)	1621, 1940
AccI	GTMKAC	1 cut(s)	1578
AccII	CGCG	7 cut(s)	476, 505, 577, 579, 581, 626, 656
AccIII	TCCGGA	1 cut(s)	766
AclWI	GGATC	5 cut(s)	481, 943, 956, 1713, 1924
AcsI	RAATTY	1 cut(s)	1656
AcuI	CTGAAG	1 cut(s)	1425
AcyI	GRCGYC	5 cut(s)	124, 408, 444, 552, 737
AfaI	GTAC	4 cut(s)	834, 964, 1809, 1902
AfiI	CCNNNNNNNGG	8 cut(s)	404, 424, 544, 829, 1488, 1621, 1846, 1940
AflII	CTTAAG	1 cut(s)	1277
AflIII	ACRYGT	1 cut(s)	801
AgeI	ACCGGT	1 cut(s)	877
AgsI	TTSAA	5 cut(s)	907, 1246, 1327, 1669, 1951
AhlI	ACTAGT	1 cut(s)	959
AjnI	CCWGG	4 cut(s)	438, 492, 1561, 1873
AjuI	GAANNNNNNNTTGG	6 cut(s)	202, 234, 1238, 1270, 1319, 1351
Alw21I	GWGCWC	2 cut(s)	995, 1158
Alw26I	GTCTC	2 cut(s)	728, 980
Alw44I	GTGCAC	1 cut(s)	1154
AlwI	GGATC	5 cut(s)	481, 943, 956, 1713, 1924
Ama87I	CYCGRG	4 cut(s)	431, 910, 977, 988
Aor13HI	TCCGGA	1 cut(s)	766
AoxI	GGCC	7 cut(s)	426, 495, 586, 616, 1359, 1478, 1877
ApaLI	GTGCAC	1 cut(s)	1154
ApeKI	GCWGC	8 cut(s)	82, 347, 560, 1195, 1535, 1547, 1553, 1814
ApoI	RAATTY	1 cut(s)	1656
AsiGI	ACCGGT	1 cut(s)	877
Asp718I	GGTACC	1 cut(s)	1807
AspS9I	GGNCC	7 cut(s)	427, 496, 586, 617, 775, 1479, 1877
AsuC2I	CCSGG	4 cut(s)	468, 594, 621, 1142
AsuHPI	GGTGA	9 cut(s)	127, 289, 316, 334, 439, 679, 1229, 1601, 1982
AsuNHI	GCTAGC	1 cut(s)	1136
AvaI	CYCGRG	4 cut(s)	431, 910, 977, 988
AvaII	GGWCC	1 cut(s)	775
BaeGI	GKGCMC	2 cut(s)	702, 1158
BamHI	GGATCC	1 cut(s)	948
BanI	GGYRCC	3 cut(s)	123, 407, 1807
BanII	GRGCYC	3 cut(s)	399, 995, 1732
BauI	CACGAG	2 cut(s)	419, 539
BbsI	GAAGAC	3 cut(s)	448, 547, 1842
Bbv12I	GWGCWC	2 cut(s)	995, 1158
BbvI	GCAGC	8 cut(s)	94, 334, 572, 1207, 1522, 1534, 1565, 1801
BccI	CCATC	8 cut(s)	475, 524, 670, 964, 1370, 1580, 1586, 1967
BcgI	CGANNNNNNTGC	2 cut(s)	554, 588
BciT130I	CCWGG	4 cut(s)	440, 494, 1563, 1875
BcnI	CCSGG	4 cut(s)	468, 594, 621, 1142
BcoDI	GTCTC	2 cut(s)	728, 980
BcuI	ACTAGT	1 cut(s)	959
BfaI	CTAG	3 cut(s)	807, 960, 1137
BfmI	CTRYAG	2 cut(s)	1016, 1173
BfoI	RGCGCY	3 cut(s)	127, 411, 439
BfrI	CTTAAG	1 cut(s)	1277
BglI	GCCNNNNNGGC	1 cut(s)	594
BglII	AGATCT	1 cut(s)	1767
BmcAI	AGTACT	1 cut(s)	964
Bme1390I	CCNGG	8 cut(s)	440, 468, 494, 594, 621, 1142, 1563, 1875
Bme18I	GGWCC	1 cut(s)	775
BmeT110I	CYCGRG	4 cut(s)	431, 910, 977, 988
BmgT120I	GGNCC	7 cut(s)	427, 496, 586, 617, 775, 1479, 1877
BmiI	GGNNCC	9 cut(s)	125, 409, 619, 765, 776, 950, 1481, 1513, 1809
BmrFI	CCNGG	8 cut(s)	440, 468, 494, 594, 621, 1142, 1563, 1875
BmrI	ACTGGG	4 cut(s)	819, 947, 954, 1125
BmsI	GCATC	3 cut(s)	712, 1011, 1020
BmtI	GCTAGC	1 cut(s)	1140
BmuI	ACTGGG	4 cut(s)	819, 947, 954, 1125
BpiI	GAAGAC	3 cut(s)	448, 547, 1842
BplI	GAGNNNNNCTC	2 cut(s)	59, 91
BpmI	CTGGAG	4 cut(s)	112, 339, 860, 1145
BpuEI	CTTGAG	1 cut(s)	137
BpuMI	CCSGG	4 cut(s)	468, 594, 621, 1142
BsaBI	GATNNNNATC	1 cut(s)	485
BsaHI	GRCGYC	5 cut(s)	124, 408, 444, 552, 737
BsaI	GGTCTC	1 cut(s)	980
BsaJI	CCNNGG	6 cut(s)	439, 466, 564, 619, 1515, 1874
BsaWI	WCCGGW	3 cut(s)	689, 766, 877
BsaXI	ACNNNNNCTCC	6 cut(s)	145, 175, 334, 364, 977, 1007
Bsc4I	CCNNNNNNNGG	8 cut(s)	404, 424, 544, 829, 1488, 1621, 1846, 1940
Bse118I	RCCGGY	1 cut(s)	877
Bse1I	ACTGG	7 cut(s)	95, 799, 825, 949, 953, 1120, 1432
Bse3DI	GCAATG	1 cut(s)	603
Bse8I	GATNNNNATC	1 cut(s)	485
BseAI	TCCGGA	1 cut(s)	766
BseBI	CCWGG	4 cut(s)	440, 494, 1563, 1875
BseDI	CCNNGG	6 cut(s)	439, 466, 564, 619, 1515, 1874
BseGI	GGATG	4 cut(s)	516, 714, 1035, 1362
BseJI	GATNNNNATC	1 cut(s)	485
BseLI	CCNNNNNNNGG	8 cut(s)	404, 424, 544, 829, 1488, 1621, 1846, 1940
BseMI	GCAATG	1 cut(s)	603
BseMII	CTCAG	3 cut(s)	1385, 1755, 1956
BseNI	ACTGG	7 cut(s)	95, 799, 825, 949, 953, 1120, 1432
BsePI	GCGCGC	2 cut(s)	575, 577
BseRI	GAGGAG	3 cut(s)	342, 932, 1359
BseSI	GKGCMC	2 cut(s)	702, 1158
BseXI	GCAGC	8 cut(s)	94, 334, 572, 1207, 1522, 1534, 1565, 1801
BseYI	CCCAGC	2 cut(s)	361, 1750
BsgI	GTGCAG	1 cut(s)	94
Bsh1236I	CGCG	7 cut(s)	476, 505, 577, 579, 581, 626, 656
BshFI	GGCC	7 cut(s)	428, 497, 588, 618, 1361, 1480, 1879
BshNI	GGYRCC	3 cut(s)	123, 407, 1807
BshTI	ACCGGT	1 cut(s)	877
BsiHKAI	GWGCWC	2 cut(s)	995, 1158
BsiHKCI	CYCGRG	4 cut(s)	431, 910, 977, 988
BsiSI	CCGG	9 cut(s)	468, 593, 615, 621, 690, 706, 767, 878, 1141
BslFI	GGGAC	2 cut(s)	788, 994
BslI	CCNNNNNNNGG	8 cut(s)	404, 424, 544, 829, 1488, 1621, 1846, 1940
BsmAI	GTCTC	2 cut(s)	728, 980
BsmBI	CGTCTC	1 cut(s)	728
BsmFI	GGGAC	2 cut(s)	788, 994
BsmI	GAATGC	1 cut(s)	669
BsnI	GGCC	7 cut(s)	428, 497, 588, 618, 1361, 1480, 1879
Bso31I	GGTCTC	1 cut(s)	980
BsoBI	CYCGRG	4 cut(s)	431, 910, 977, 988
Bsp1286I	GDGCHC	5 cut(s)	399, 702, 995, 1158, 1732
Bsp13I	TCCGGA	1 cut(s)	766
Bsp143I	GATC	7 cut(s)	486, 711, 948, 1105, 1705, 1767, 1929
Bsp19I	CCATGG	1 cut(s)	1515
BspANI	GGCC	7 cut(s)	428, 497, 588, 618, 1361, 1480, 1879
BspCNI	CTCAG	3 cut(s)	1384, 1756, 1957
BspEI	TCCGGA	1 cut(s)	766
BspFNI	CGCG	7 cut(s)	476, 505, 577, 579, 581, 626, 656
BspLI	GGNNCC	9 cut(s)	125, 409, 619, 765, 776, 950, 1481, 1513, 1809
BspMAI	CTGCAG	2 cut(s)	1020, 1177
BspOI	GCTAGC	1 cut(s)	1140
BspPI	GGATC	5 cut(s)	481, 943, 956, 1713, 1924
BspT107I	GGYRCC	3 cut(s)	123, 407, 1807
BspTI	CTTAAG	1 cut(s)	1277
BspTNI	GGTCTC	1 cut(s)	980
BsrDI	GCAATG	1 cut(s)	603
BsrFI	RCCGGY	1 cut(s)	877
BsrI	ACTGG	7 cut(s)	95, 799, 825, 949, 953, 1120, 1432
BssAI	RCCGGY	1 cut(s)	877
BssECI	CCNNGG	6 cut(s)	439, 466, 564, 619, 1515, 1874
BssHII	GCGCGC	2 cut(s)	575, 577
BssMI	GATC	7 cut(s)	486, 711, 948, 1105, 1705, 1767, 1929
BssNI	GRCGYC	5 cut(s)	124, 408, 444, 552, 737
BssSI	CACGAG	2 cut(s)	419, 539
BssT1I	CCWWGG	1 cut(s)	1515
Bst2BI	CACGAG	2 cut(s)	419, 539
Bst2UI	CCWGG	4 cut(s)	440, 494, 1563, 1875
Bst4CI	ACNGT	3 cut(s)	454, 1042, 1488
Bst6I	CTCTTC	3 cut(s)	82, 175, 198
BstACI	GRCGYC	5 cut(s)	124, 408, 444, 552, 737
BstAFI	CTTAAG	1 cut(s)	1277
BstAPI	GCANNNNNTGC	1 cut(s)	1921
BstC8I	GCNNGC	5 cut(s)	577, 579, 1083, 1138, 1533
BstDEI	CTNAG	5 cut(s)	1146, 1371, 1475, 1764, 1965
BstDSI	CCRYGG	1 cut(s)	1515
BstENI	CCTNNNNNAGG	1 cut(s)	422
BstF5I	GGATG	4 cut(s)	516, 714, 1035, 1362
BstFNI	CGCG	7 cut(s)	476, 505, 577, 579, 581, 626, 656
BstH2I	RGCGCY	3 cut(s)	127, 411, 439
BstKTI	GATC	7 cut(s)	489, 714, 951, 1108, 1708, 1770, 1932
BstMAI	GTCTC	2 cut(s)	728, 980
BstMBI	GATC	7 cut(s)	486, 711, 948, 1105, 1705, 1767, 1929
BstNI	CCWGG	4 cut(s)	440, 494, 1563, 1875
BstNSI	RCATGY	2 cut(s)	805, 1919
BstSCI	CCNGG	8 cut(s)	438, 466, 492, 592, 619, 1140, 1561, 1873
BstSFI	CTRYAG	2 cut(s)	1016, 1173
BstSLI	GKGCMC	2 cut(s)	702, 1158
BstUI	CGCG	7 cut(s)	476, 505, 577, 579, 581, 626, 656
BstV1I	GCAGC	8 cut(s)	94, 334, 572, 1207, 1522, 1534, 1565, 1801
BstV2I	GAAGAC	3 cut(s)	448, 547, 1842
BstX2I	RGATCY	3 cut(s)	486, 948, 1767
BstYI	RGATCY	3 cut(s)	486, 948, 1767
BsuRI	GGCC	7 cut(s)	428, 497, 588, 618, 1361, 1480, 1879
BtgI	CCRYGG	1 cut(s)	1515
BtsCI	GGATG	4 cut(s)	516, 714, 1035, 1362
BtsI	GCAGTG	1 cut(s)	1013
BtsIMutI	CAGTG	7 cut(s)	88, 450, 832, 1013, 1425, 1493, 1703
Cac8I	GCNNGC	5 cut(s)	577, 579, 1083, 1138, 1533
Cfr10I	RCCGGY	1 cut(s)	877
Cfr13I	GGNCC	7 cut(s)	427, 496, 586, 617, 775, 1479, 1877
CseI	GACGC	6 cut(s)	433, 465, 541, 570, 662, 745
Csp6I	GTAC	4 cut(s)	833, 963, 1808, 1901
CspAI	ACCGGT	1 cut(s)	877
CspCI	CAANNNNNGTGG	2 cut(s)	1617, 1652
CviAII	CATG	6 cut(s)	661, 802, 1034, 1202, 1516, 1916
CviQI	GTAC	4 cut(s)	833, 963, 1808, 1901
DdeI	CTNAG	5 cut(s)	1146, 1371, 1475, 1764, 1965
DinI	GGCGCC	2 cut(s)	125, 409
DpnI	GATC	7 cut(s)	488, 713, 950, 1107, 1707, 1769, 1931
DpnII	GATC	7 cut(s)	486, 711, 948, 1105, 1705, 1767, 1929
DraI	TTTAAA	1 cut(s)	12
Eam1104I	CTCTTC	3 cut(s)	82, 175, 198
EarI	CTCTTC	3 cut(s)	82, 175, 198
EciI	GGCGGA	2 cut(s)	260, 1319
Ecl136II	GAGCTC	1 cut(s)	993
Eco130I	CCWWGG	1 cut(s)	1515
Eco24I	GRGCYC	3 cut(s)	399, 995, 1732
Eco31I	GGTCTC	1 cut(s)	980
Eco47I	GGWCC	1 cut(s)	775
Eco53kI	GAGCTC	1 cut(s)	993
Eco57I	CTGAAG	1 cut(s)	1425
Eco88I	CYCGRG	4 cut(s)	431, 910, 977, 988
EcoICRI	GAGCTC	1 cut(s)	993
EcoNI	CCTNNNNNAGG	1 cut(s)	422
EcoO109I	RGGNCCY	3 cut(s)	427, 496, 1479
EcoRII	CCWGG	4 cut(s)	438, 492, 1561, 1873
EcoT14I	CCWWGG	1 cut(s)	1515
EcoT22I	ATGCAT	2 cut(s)	605, 1035
EcoT38I	GRGCYC	3 cut(s)	399, 995, 1732
EgeI	GGCGCC	2 cut(s)	125, 409
EheI	GGCGCC	2 cut(s)	125, 409
ErhI	CCWWGG	1 cut(s)	1515
Esp3I	CGTCTC	1 cut(s)	728
FaeI	CATG	6 cut(s)	664, 805, 1037, 1205, 1519, 1919
FalI	AAGNNNNNCTT	2 cut(s)	1058, 1090
FaqI	GGGAC	2 cut(s)	788, 994
FatI	CATG	6 cut(s)	660, 801, 1033, 1201, 1515, 1915
FauI	CCCGC	2 cut(s)	542, 710
FblI	GTMKAC	1 cut(s)	1578
FokI	GGATG	4 cut(s)	503, 721, 1042, 1349
FriOI	GRGCYC	3 cut(s)	399, 995, 1732
FspBI	CTAG	3 cut(s)	807, 960, 1137
GsaI	CCCAGC	2 cut(s)	365, 1754
GsuI	CTGGAG	4 cut(s)	112, 339, 860, 1145
HaeII	RGCGCY	3 cut(s)	127, 411, 439
HaeIII	GGCC	7 cut(s)	428, 497, 588, 618, 1361, 1480, 1879
HapII	CCGG	9 cut(s)	468, 593, 615, 621, 690, 706, 767, 878, 1141
HgaI	GACGC	6 cut(s)	433, 465, 541, 570, 662, 745
Hin1I	GRCGYC	5 cut(s)	124, 408, 444, 552, 737
Hin1II	CATG	6 cut(s)	664, 805, 1037, 1205, 1519, 1919
HincII	GTYRAC	5 cut(s)	144, 792, 1401, 1494, 1579
HindII	GTYRAC	5 cut(s)	144, 792, 1401, 1494, 1579
HindIII	AAGCTT	3 cut(s)	1274, 1280, 1797
HinfI	GANTC	4 cut(s)	866, 1169, 1642, 1760
HpaI	GTTAAC	2 cut(s)	792, 1401
HpaII	CCGG	9 cut(s)	468, 593, 615, 621, 690, 706, 767, 878, 1141
HphI	GGTGA	9 cut(s)	127, 289, 316, 334, 439, 679, 1229, 1601, 1982
Hpy166II	GTNNAC	8 cut(s)	62, 144, 792, 1063, 1156, 1401, 1494, 1579
Hpy188I	TCNGA	6 cut(s)	675, 716, 865, 997, 1446, 1765
Hpy8I	GTNNAC	8 cut(s)	62, 144, 792, 1063, 1156, 1401, 1494, 1579
Hpy99I	CGWCG	3 cut(s)	481, 586, 656
HpyAV	CCTTC	6 cut(s)	187, 277, 599, 1400, 1405, 1825
HpyCH4III	ACNGT	3 cut(s)	454, 1042, 1488
HpyCH4IV	ACGT	2 cut(s)	525, 1893
HpyCH4V	TGCA	7 cut(s)	111, 603, 1018, 1033, 1156, 1175, 1237
HpyF3I	CTNAG	5 cut(s)	1146, 1371, 1475, 1764, 1965
HpySE526I	ACGT	2 cut(s)	525, 1893
Hsp92I	GRCGYC	5 cut(s)	124, 408, 444, 552, 737
Hsp92II	CATG	6 cut(s)	664, 805, 1037, 1205, 1519, 1919
KasI	GGCGCC	2 cut(s)	123, 407
Kpn2I	TCCGGA	1 cut(s)	766
KpnI	GGTACC	1 cut(s)	1811
KspAI	GTTAAC	2 cut(s)	792, 1401
Kzo9I	GATC	7 cut(s)	486, 711, 948, 1105, 1705, 1767, 1929
LmnI	GCTCC	9 cut(s)	93, 166, 355, 512, 841, 937, 998, 1306, 1511
Lsp1109I	GCAGC	8 cut(s)	94, 334, 572, 1207, 1522, 1534, 1565, 1801
LweI	GCATC	3 cut(s)	712, 1011, 1020
MaeI	CTAG	3 cut(s)	807, 960, 1137
MaeII	ACGT	2 cut(s)	525, 1893
MaeIII	GTNAC	9 cut(s)	322, 445, 797, 955, 1011, 1111, 1340, 1822, 1985
MalI	GATC	7 cut(s)	488, 713, 950, 1107, 1707, 1769, 1931
MauBI	CGCGCGCG	1 cut(s)	577
MboI	GATC	7 cut(s)	486, 711, 948, 1105, 1705, 1767, 1929
MflI	RGATCY	3 cut(s)	486, 948, 1767
MhlI	GDGCHC	5 cut(s)	399, 702, 995, 1158, 1732
MluCI	AATT	6 cut(s)	30, 372, 1265, 1322, 1619, 1656
Mly113I	GGCGCC	2 cut(s)	124, 408
MlyI	GAGTC	2 cut(s)	875, 1769
MmeI	TCCRAC	4 cut(s)	312, 843, 1845, 1869
Mph1103I	ATGCAT	2 cut(s)	605, 1035
MroI	TCCGGA	1 cut(s)	766
MseI	TTAA	5 cut(s)	11, 33, 791, 1278, 1400
MslI	CAYNNNNRTG	1 cut(s)	674
MspA1I	CMGCKG	4 cut(s)	347, 642, 1535, 1814
MspCI	CTTAAG	1 cut(s)	1277
MspI	CCGG	9 cut(s)	468, 593, 615, 621, 690, 706, 767, 878, 1141
MspR9I	CCNGG	8 cut(s)	440, 468, 494, 594, 621, 1142, 1563, 1875
Mva1269I	GAATGC	1 cut(s)	669
MvaI	CCWGG	4 cut(s)	440, 494, 1563, 1875
MvnI	CGCG	7 cut(s)	476, 505, 577, 579, 581, 626, 656
NarI	GGCGCC	2 cut(s)	124, 408
NciI	CCSGG	4 cut(s)	468, 594, 621, 1142
NcoI	CCATGG	1 cut(s)	1515
NdeII	GATC	7 cut(s)	486, 711, 948, 1105, 1705, 1767, 1929
NheI	GCTAGC	1 cut(s)	1136
NlaIII	CATG	6 cut(s)	664, 805, 1037, 1205, 1519, 1919
NlaIV	GGNNCC	9 cut(s)	125, 409, 619, 765, 776, 950, 1481, 1513, 1809
NmeAIII	GCCGAG	1 cut(s)	589
NmuCI	GTSAC	5 cut(s)	322, 445, 1011, 1111, 1822
NsiI	ATGCAT	2 cut(s)	605, 1035
NspI	RCATGY	2 cut(s)	805, 1919
PaeR7I	CTCGAG	2 cut(s)	431, 910
PauI	GCGCGC	2 cut(s)	575, 577
PciI	ACATGT	1 cut(s)	801
PctI	GAATGC	1 cut(s)	669
PfeI	GAWTC	2 cut(s)	1169, 1642
PflMI	CCANNNNNTGG	2 cut(s)	1621, 1940
PinAI	ACCGGT	1 cut(s)	877
PleI	GAGTC	2 cut(s)	874, 1768
PluTI	GGCGCC	2 cut(s)	127, 411
PpsI	GAGTC	2 cut(s)	874, 1768
PscI	ACATGT	1 cut(s)	801
PsiI	TTATAA	1 cut(s)	1421
Psp124BI	GAGCTC	1 cut(s)	995
Psp6I	CCWGG	4 cut(s)	438, 492, 1561, 1873
PspFI	CCCAGC	2 cut(s)	361, 1750
PspGI	CCWGG	4 cut(s)	438, 492, 1561, 1873
PspN4I	GGNNCC	9 cut(s)	125, 409, 619, 765, 776, 950, 1481, 1513, 1809
PspPI	GGNCC	7 cut(s)	427, 496, 586, 617, 775, 1479, 1877
PspXI	VCTCGAGB	2 cut(s)	431, 910
PstI	CTGCAG	2 cut(s)	1020, 1177
PsuI	RGATCY	3 cut(s)	486, 948, 1767
PteI	GCGCGC	2 cut(s)	575, 577
PvuII	CAGCTG	2 cut(s)	1535, 1814
RsaI	GTAC	4 cut(s)	834, 964, 1809, 1902
RsaNI	GTAC	4 cut(s)	833, 963, 1808, 1901
RseI	CAYNNNNRTG	1 cut(s)	674
SacI	GAGCTC	1 cut(s)	995
SalI	GTCGAC	1 cut(s)	1577
SaqAI	TTAA	5 cut(s)	11, 33, 791, 1278, 1400
Sau3AI	GATC	7 cut(s)	486, 711, 948, 1105, 1705, 1767, 1929
Sau96I	GGNCC	7 cut(s)	427, 496, 586, 617, 775, 1479, 1877
ScaI	AGTACT	1 cut(s)	964
SchI	GAGTC	2 cut(s)	875, 1769
ScrFI	CCNGG	8 cut(s)	440, 468, 494, 594, 621, 1142, 1563, 1875
SduI	GDGCHC	5 cut(s)	399, 702, 995, 1158, 1732
SfaNI	GCATC	3 cut(s)	712, 1011, 1020
SfcI	CTRYAG	2 cut(s)	1016, 1173
SfoI	GGCGCC	2 cut(s)	125, 409
Sfr274I	CTCGAG	2 cut(s)	431, 910
SinI	GGWCC	1 cut(s)	775
SlaI	CTCGAG	2 cut(s)	431, 910
SmiMI	CAYNNNNRTG	1 cut(s)	674
SmlI	CTYRAG	4 cut(s)	116, 431, 910, 1277
SmoI	CTYRAG	4 cut(s)	116, 431, 910, 1277
SpeI	ACTAGT	1 cut(s)	959
Sse9I	AATT	6 cut(s)	30, 372, 1265, 1322, 1619, 1656
SspDI	GGCGCC	2 cut(s)	123, 407
SspI	AATATT	1 cut(s)	8
SspMI	CTAG	3 cut(s)	807, 960, 1137
SstI	GAGCTC	1 cut(s)	995
StyD4I	CCNGG	8 cut(s)	438, 466, 492, 592, 619, 1140, 1561, 1873
StyI	CCWWGG	1 cut(s)	1515
TaaI	ACNGT	3 cut(s)	454, 1042, 1488
TaiI	ACGT	2 cut(s)	528, 1896
TaqI	TCGA	5 cut(s)	432, 479, 869, 911, 1578
TasI	AATT	6 cut(s)	30, 372, 1265, 1322, 1619, 1656
TatI	WGTACW	1 cut(s)	962
TauI	GCSGC	5 cut(s)	474, 552, 1457, 1553, 1850
TfiI	GAWTC	2 cut(s)	1169, 1642
Tru1I	TTAA	5 cut(s)	11, 33, 791, 1278, 1400
Tru9I	TTAA	5 cut(s)	11, 33, 791, 1278, 1400
TscAI	CASTG	7 cut(s)	95, 457, 832, 1020, 1432, 1493, 1703
TseFI	GTSAC	5 cut(s)	322, 445, 1011, 1111, 1822
TseI	GCWGC	8 cut(s)	82, 347, 560, 1195, 1535, 1547, 1553, 1814
Tsp45I	GTSAC	5 cut(s)	322, 445, 1011, 1111, 1822
TspDTI	ATGAA	3 cut(s)	17, 1014, 1622
TspRI	CASTG	7 cut(s)	95, 457, 832, 1020, 1432, 1493, 1703
Van91I	CCANNNNNTGG	2 cut(s)	1621, 1940
Vha464I	CTTAAG	1 cut(s)	1277
VneI	GTGCAC	1 cut(s)	1154
VpaK11BI	GGWCC	1 cut(s)	775
XagI	CCTNNNNNAGG	1 cut(s)	422
XapI	RAATTY	1 cut(s)	1656
XceI	RCATGY	2 cut(s)	805, 1919
XhoI	CTCGAG	2 cut(s)	431, 910
XmiI	GTMKAC	1 cut(s)	1578
XspI	CTAG	3 cut(s)	807, 960, 1137
ZrmI	AGTACT	1 cut(s)	964
Zsp2I	ATGCAT	2 cut(s)	605, 1035

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.