Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006113

Physical Location & Seq

Genomic Coordinates Reverse (-)

1850632 .. 1852629

Gene Structure 1998 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1013700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1998 bp

ATGTCAAAAATGGCGGCCATCTCTCCATCCCCTTCCTCCTCCTTCTCCAAGCCCTCTCCTCCTCCTTTGCCTTCTTCCCAAAATACCATTTCCGGATTTACTTTACCCTTTTCCCCCATTCCCCACAGATCCACTCCCTTCCGCCCTCTCCGCATCTCCAATTCCCTCAAACCCAAACCCACACCCAAGCCGACCGCCGCCGCCGCCGCCACTACCGCCACTACCCCCTCCGATCCCCAAACTTTCACTTCCCGATATGCCCCTGACGAGCCCAGAAAAGGCGCTGATGTCCTCGTGGAGGCCCTCGAACGCCAAGGCGTCACCGATGTATTCGCCTATCCTGGAGGAGCTTCCATGGAGATCCACCAGGCCCTCACCCGCTCCTCCGTCATCCGCAACGTCCTCCCCCGCCACGAGCAAGGCGGCATCTTCGCTGCCGAGGGATATGCGCGCGCCTCCGGCCTCCCCGGCGTCTGCATCGCCACCTCCGGCCCCGGCGCCACCAACTTGGTCAGTGGCCTCGCCGACGCGCTCCTCGACAGCGTGCCGTTGGTGGCAATTACGGGACAGGTCCCCCGCCGCATGATCGGCACCGACGCATTTCAAGAGACTCCGATTGTAGAGGTAACGAGATCCATCACCAAGCACAATTATCTTGTTCTTGATGTAGAAGAAATCCCTAGGATTGTCAGCGAGGCATTTTTCTTGGCCAGCTCGGGTCGGCCCGGGCCGGTTTTGATAGACATCCCGAAAGATATACAGCAACAGCTTGTGGTTCCCAATTGGAACCAACCCATGAGTTTGCCTGGCTACATGTCTAGGTTGCCCAAGTCCCCCTCCAACTCCCACCTGGACCAGATTGTTAGGTTGGTCTCTGAGTCCAAGAGGCCCGTTTTGTATGTTGGCGGCGGCTGTTTGAACTCTAGCGAGGAGTTGAGGCGCTTCGTGGAGCTTACCGGGATCCCCGTTGCCAGTACTTTGATGGGTCTCGGTGCCTTTCCGAGCTCCAACGAATTGTCCCTCCAAATGCTTGGAATGCACGGCACTGTGTGCGCTAATTATTCTGTGGATAAGTCTGACTTGTTGCTTGCCTTTGGGGTGAGGTTCGATGATCGTGTCACGGGCAAGCTCGAAGCTTTTGCTAGCCGAGCAAAGATTGTCCACATTGACATTGACTCTGCAGAGATTGGAAAGAACAAGCAGCCTCATGTCTCGGTTTGTGCGGATGTGAAATTGGCACTGGCAGGGATGAACCGGACATTGGAAAACAAAGGAAGCAAGCTTAAACTTGATTTCTCGGCTTGGAGGGAGGAGTTGAATGAGCAGAAAGTGAAGTATCCGTTGAGTTTTAAGACTTTTGGGGAAGCCATTCCTCCACAGTATGCGATTCAGGTTCTCGATGAATTAACTGATGGGAGTGCAATTATAAGCACCGGTGTTGGGCAGCATCAAATGTGGGCGGCTCAGTTTTATAAATACAAGAGGCCTCGCCAGTGGTTGACATCCGCGGGATTAGGAGCCATGGGTTTTGGATTGCCTGCTGCTATTGGGGCCGCGGTTGCAAATCCAGATGCGATTGTTGTTGACATTGATGGCGATGGAAGTTTCATGATGAATGTCCAGGAATTGGCAACCATAAGAGTGGAGAATCTGCCAGTGAAGATAATGCTGTTGAATAATCAGCATTTGGGAATGGTGGTTCAGTGGGAGGATCGGTTCTATAAGGCAAACCGGGCTCACACATACTTAGGGAACCCGTCAAATGAATCGGAGATATTCCCTAATATGCTGAAGTTTGCAGAGGCTTGCGGGATACCAGCTGCCCGGGTGACGAAGAAGGAAAATCTGAAGGAGGCAATTCAGAAAATGCTGGACACTCCTGGTCCGTACTTGTTGGATGTAATAGTACCCCATCAAGAGCATGTGTTGCCTATGATCCCCAGCGGCGGTGCCTTCAAAGATGTGATCACCGAAGGTGATGGAAGATCGTCTTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009099 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

666

Amino Acids

71.89

Weight (kDa)

7.22

Isoelectric Point (pI)

44.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	93 - 203	4e-36	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	284 - 415	3.3e-45	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	479 - 634	2.2e-51	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48560
fragaria_vesca	FvH4_7g06770
malus_domestica	MD06G1028700.v1.1 MD10G1013700.v1.1
prunus_persica	Prupe.5G043100_v2.0.a1 Prupe.8G001300_v2.0.a1 Prupe.8G001300_v2.0.a1
pyrus_communis	pycom06g02280 pycom10g00840
rosa_chinensis	RchiOBHm_Chr7g0208791
rosa_laevigata	RLG00000003195 RLG00000015583
rosa_multiflora	Rmu_co8493263.1_g000001 Rmu_sc0000278.1_g000002 Rmu_sc0008663.1_g000001 Rmu_sc0018750.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250260 Rroxscaffold_7G00217760
rosa_rugosa	Rorug05G0492000 Rorug07G0105800
rosa_samantha	Rh6AG001800 Rh7AG241000 Rh7BG235900 Rh7CG255600 Rh7DG247000
rosa_wichuraiana	Rw6G000240 Rw7G020440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1427, 1473
AccB1I	GGYRCC	4 cut(s)	497, 590, 992, 1949
AccB7I	CCANNNNNTGG	1 cut(s)	1627
AccBSI	CCGCTC	1 cut(s)	381
AccII	CGCG	5 cut(s)	451, 453, 530, 1508, 1556
AccIII	TCCGGA	1 cut(s)	92
AclWI	GGATC	8 cut(s)	123, 227, 355, 627, 955, 968, 1719, 1930
AcoI	YGGCCR	2 cut(s)	15, 708
AcuI	CTGAAG	2 cut(s)	1811, 1868
AcyI	GRCGYC	3 cut(s)	318, 471, 498
AdeI	CACNNNGTG	1 cut(s)	1050
AfaI	GTAC	3 cut(s)	976, 1889, 1908
AfiI	CCNNNNNNNGG	6 cut(s)	278, 298, 1261, 1440, 1627, 1946
AflIII	ACRYGT	1 cut(s)	813
AgeI	ACCGGT	1 cut(s)	1433
AgsI	TTSAA	5 cut(s)	605, 919, 1318, 1675, 1957
AjnI	CCWGG	6 cut(s)	340, 366, 805, 849, 1620, 1879
AjuI	GAANNNNNNNTTGG	2 cut(s)	1244, 1276
AloI	GAACNNNNNNTCC	4 cut(s)	1683, 1700, 1715, 1732
AluBI	AGCT	9 cut(s)	350, 714, 769, 952, 1005, 1129, 1136, 1282, 1820
AluI	AGCT	9 cut(s)	350, 714, 769, 952, 1005, 1129, 1136, 1282, 1820
Alw21I	GWGCWC	1 cut(s)	1007
Alw26I	GTCTC	4 cut(s)	602, 877, 992, 1216
AlwI	GGATC	8 cut(s)	123, 227, 355, 627, 955, 968, 1719, 1930
Ama87I	CYCGRG	3 cut(s)	715, 725, 1824
Aor13HI	TCCGGA	1 cut(s)	92
ApeKI	GCWGC	5 cut(s)	434, 1201, 1444, 1541, 1820
AsiGI	ACCGGT	1 cut(s)	1433
Asp700I	GAANNNNTTC	1 cut(s)	1368
AspA2I	CCTAGG	1 cut(s)	680
AspLEI	GCGC	8 cut(s)	284, 451, 453, 455, 500, 532, 942, 1055
AsuC2I	CCSGG	8 cut(s)	468, 495, 726, 727, 958, 1733, 1825, 1826
AsuHPI	GGTGA	7 cut(s)	313, 367, 631, 1111, 1840, 1960, 1988
AsuNHI	GCTAGC	1 cut(s)	1142
AvaI	CYCGRG	3 cut(s)	715, 725, 1824
AvaII	GGWCC	3 cut(s)	571, 853, 1883
AvrII	CCTAGG	1 cut(s)	680
BalI	TGGCCA	1 cut(s)	710
BamHI	GGATCC	1 cut(s)	960
BanI	GGYRCC	4 cut(s)	497, 590, 992, 1949
BanII	GRGCYC	3 cut(s)	273, 1007, 1738
BauI	CACGAG	2 cut(s)	293, 413
Bbv12I	GWGCWC	1 cut(s)	1007
BbvI	GCAGC	5 cut(s)	421, 1213, 1456, 1528, 1807
BccI	CCATC	9 cut(s)	26, 34, 644, 976, 1406, 1586, 1592, 1920, 1973
BceAI	ACGGC	2 cut(s)	532, 1057
BcgI	CGANNNNNNTGC	4 cut(s)	428, 462, 1121, 1155
BciT130I	CCWGG	6 cut(s)	342, 368, 807, 851, 1622, 1881
BciVI	GTATCC	2 cut(s)	1347, 1806
BclI	TGATCA	1 cut(s)	1965
BcnI	CCSGG	8 cut(s)	468, 495, 726, 727, 958, 1733, 1825, 1826
BcoDI	GTCTC	4 cut(s)	602, 877, 992, 1216
BfaI	CTAG	4 cut(s)	681, 819, 924, 1143
BfmI	CTRYAG	1 cut(s)	1179
BfoI	RGCGCY	3 cut(s)	285, 501, 943
BfuI	GTATCC	2 cut(s)	1347, 1806
BglI	GCCNNNNNGGC	1 cut(s)	468
BlnI	CCTAGG	1 cut(s)	680
BmcAI	AGTACT	1 cut(s)	976
Bme18I	GGWCC	3 cut(s)	571, 853, 1883
BmeT110I	CYCGRG	3 cut(s)	715, 725, 1824
BmsI	GCATC	5 cut(s)	162, 435, 486, 1456, 1561
BmtI	GCTAGC	1 cut(s)	1146
BpmI	CTGGAG	1 cut(s)	363
BpuMI	CCSGG	8 cut(s)	468, 495, 726, 727, 958, 1733, 1825, 1826
BsaBI	GATNNNNATC	1 cut(s)	743
BsaHI	GRCGYC	3 cut(s)	318, 471, 498
BsaI	GGTCTC	2 cut(s)	877, 992
BsaWI	WCCGGW	3 cut(s)	92, 1254, 1433
BsaXI	ACNNNNNCTCC	2 cut(s)	1700, 1730
Bsc4I	CCNNNNNNNGG	6 cut(s)	278, 298, 1261, 1440, 1627, 1946
Bse118I	RCCGGY	2 cut(s)	730, 1433
Bse1I	ACTGG	4 cut(s)	972, 1245, 1492, 1655
Bse8I	GATNNNNATC	1 cut(s)	743
BseAI	TCCGGA	1 cut(s)	92
BseBI	CCWGG	6 cut(s)	342, 368, 807, 851, 1622, 1881
BseGI	GGATG	7 cut(s)	26, 390, 744, 1231, 1254, 1502, 1903
BseJI	GATNNNNATC	1 cut(s)	743
BseLI	CCNNNNNNNGG	6 cut(s)	278, 298, 1261, 1440, 1627, 1946
BseMII	CTCAG	2 cut(s)	867, 1478
BseNI	ACTGG	4 cut(s)	972, 1245, 1492, 1655
BsePI	GCGCGC	2 cut(s)	449, 451
BseRI	GAGGAG	8 cut(s)	28, 48, 51, 360, 373, 524, 944, 1325
BseXI	GCAGC	5 cut(s)	421, 1213, 1456, 1528, 1807
BseYI	CCCAGC	1 cut(s)	1940
Bsh1236I	CGCG	5 cut(s)	451, 453, 530, 1508, 1556
Bsh1285I	CGRYCG	1 cut(s)	195
BshNI	GGYRCC	4 cut(s)	497, 590, 992, 1949
BshTI	ACCGGT	1 cut(s)	1433
BsiEI	CGRYCG	1 cut(s)	195
BsiHKAI	GWGCWC	1 cut(s)	1007
BsiHKCI	CYCGRG	3 cut(s)	715, 725, 1824
BslFI	GGGAC	4 cut(s)	557, 579, 817, 1003
BslI	CCNNNNNNNGG	6 cut(s)	278, 298, 1261, 1440, 1627, 1946
BsmAI	GTCTC	4 cut(s)	602, 877, 992, 1216
BsmFI	GGGAC	4 cut(s)	557, 579, 817, 1003
BsmI	GAATGC	1 cut(s)	1041
Bso31I	GGTCTC	2 cut(s)	877, 992
BsoBI	CYCGRG	3 cut(s)	715, 725, 1824
Bsp1286I	GDGCHC	3 cut(s)	273, 1007, 1738
Bsp13I	TCCGGA	1 cut(s)	92
Bsp19I	CCATGG	2 cut(s)	354, 1521
BspCNI	CTCAG	2 cut(s)	868, 1477
BspEI	TCCGGA	1 cut(s)	92
BspFNI	CGCG	5 cut(s)	451, 453, 530, 1508, 1556
BspHI	TCATGA	1 cut(s)	1608
BspMAI	CTGCAG	1 cut(s)	1183
BspOI	GCTAGC	1 cut(s)	1146
BspPI	GGATC	8 cut(s)	123, 227, 355, 627, 955, 968, 1719, 1930
BspT107I	GGYRCC	4 cut(s)	497, 590, 992, 1949
BspTNI	GGTCTC	2 cut(s)	877, 992
BsrBI	CCGCTC	1 cut(s)	381
BsrFI	RCCGGY	2 cut(s)	730, 1433
BsrI	ACTGG	4 cut(s)	972, 1245, 1492, 1655
BssAI	RCCGGY	2 cut(s)	730, 1433
BssHII	GCGCGC	2 cut(s)	449, 451
BssNI	GRCGYC	3 cut(s)	318, 471, 498
BssSI	CACGAG	2 cut(s)	293, 413
BssT1I	CCWWGG	4 cut(s)	313, 354, 680, 1521
Bst2BI	CACGAG	2 cut(s)	293, 413
Bst2UI	CCWGG	6 cut(s)	342, 368, 807, 851, 1622, 1881
Bst4CI	ACNGT	2 cut(s)	1048, 1380
BstACI	GRCGYC	3 cut(s)	318, 471, 498
BstAPI	GCANNNNNTGC	2 cut(s)	1050, 1927
BstDEI	CTNAG	3 cut(s)	876, 1464, 1747
BstDSI	CCRYGG	4 cut(s)	354, 1506, 1521, 1554
BstENI	CCTNNNNNAGG	1 cut(s)	296
BstF5I	GGATG	7 cut(s)	26, 390, 744, 1231, 1254, 1502, 1903
BstFNI	CGCG	5 cut(s)	451, 453, 530, 1508, 1556
BstH2I	RGCGCY	3 cut(s)	285, 501, 943
BstHHI	GCGC	8 cut(s)	284, 451, 453, 455, 500, 532, 942, 1055
BstMAI	GTCTC	4 cut(s)	602, 877, 992, 1216
BstMCI	CGRYCG	1 cut(s)	195
BstNI	CCWGG	6 cut(s)	342, 368, 807, 851, 1622, 1881
BstNSI	RCATGY	2 cut(s)	817, 1925
BstSFI	CTRYAG	1 cut(s)	1179
BstUI	CGCG	5 cut(s)	451, 453, 530, 1508, 1556
BstV1I	GCAGC	5 cut(s)	421, 1213, 1456, 1528, 1807
BstX2I	RGATCY	4 cut(s)	128, 360, 632, 960
BstXI	CCANNNNNNTGG	3 cut(s)	508, 1031, 1642
BstYI	RGATCY	4 cut(s)	128, 360, 632, 960
BsuI	GTATCC	2 cut(s)	1347, 1806
BtgI	CCRYGG	4 cut(s)	354, 1506, 1521, 1554
BtgZI	GCGATG	2 cut(s)	463, 1611
BtsCI	GGATG	7 cut(s)	26, 390, 744, 1231, 1254, 1502, 1903
BtsIMutI	CAGTG	6 cut(s)	520, 1044, 1238, 1499, 1662, 1709
CciI	TCATGA	1 cut(s)	1608
CfoI	GCGC	8 cut(s)	284, 451, 453, 455, 500, 532, 942, 1055
Cfr10I	RCCGGY	2 cut(s)	730, 1433
Cfr42I	CCGCGG	2 cut(s)	1509, 1557
Cfr9I	CCCGGG	2 cut(s)	725, 1824
CseI	GACGC	4 cut(s)	307, 460, 536, 605
Csp6I	GTAC	3 cut(s)	975, 1888, 1907
CspAI	ACCGGT	1 cut(s)	1433
CviAII	CATG	8 cut(s)	355, 583, 796, 814, 1208, 1522, 1609, 1922
CviQI	GTAC	3 cut(s)	975, 1888, 1907
DdeI	CTNAG	3 cut(s)	876, 1464, 1747
DinI	GGCGCC	1 cut(s)	499
DraIII	CACNNNGTG	1 cut(s)	1050
EaeI	YGGCCR	2 cut(s)	15, 708
EciI	GGCGGA	1 cut(s)	131
Ecl136II	GAGCTC	1 cut(s)	1005
Eco130I	CCWWGG	4 cut(s)	313, 354, 680, 1521
Eco147I	AGGCCT	1 cut(s)	1486
Eco24I	GRGCYC	3 cut(s)	273, 1007, 1738
Eco31I	GGTCTC	2 cut(s)	877, 992
Eco47I	GGWCC	3 cut(s)	571, 853, 1883
Eco53kI	GAGCTC	1 cut(s)	1005
Eco57I	CTGAAG	2 cut(s)	1811, 1868
Eco88I	CYCGRG	3 cut(s)	715, 725, 1824
EcoICRI	GAGCTC	1 cut(s)	1005
EcoNI	CCTNNNNNAGG	1 cut(s)	296
EcoO109I	RGGNCCY	3 cut(s)	301, 370, 571
EcoRII	CCWGG	6 cut(s)	340, 366, 805, 849, 1620, 1879
EcoT14I	CCWWGG	4 cut(s)	313, 354, 680, 1521
EcoT38I	GRGCYC	3 cut(s)	273, 1007, 1738
EgeI	GGCGCC	1 cut(s)	499
EheI	GGCGCC	1 cut(s)	499
ErhI	CCWWGG	4 cut(s)	313, 354, 680, 1521
FaeI	CATG	8 cut(s)	358, 586, 799, 817, 1211, 1525, 1612, 1925
FalI	AAGNNNNNCTT	3 cut(s)	1064, 1096, 1975
FaqI	GGGAC	4 cut(s)	557, 579, 817, 1003
FatI	CATG	8 cut(s)	354, 582, 795, 813, 1207, 1521, 1608, 1921
FauI	CCCGC	5 cut(s)	386, 416, 584, 1501, 1802
FbaI	TGATCA	1 cut(s)	1965
FokI	GGATG	7 cut(s)	13, 377, 731, 1238, 1261, 1489, 1910
FriOI	GRGCYC	3 cut(s)	273, 1007, 1738
FspBI	CTAG	4 cut(s)	681, 819, 924, 1143
GlaI	GCGC	8 cut(s)	283, 450, 452, 454, 499, 531, 941, 1054
GsaI	CCCAGC	1 cut(s)	1944
GsuI	CTGGAG	1 cut(s)	363
HaeII	RGCGCY	3 cut(s)	285, 501, 943
HgaI	GACGC	4 cut(s)	307, 460, 536, 605
HhaI	GCGC	8 cut(s)	284, 451, 453, 455, 500, 532, 942, 1055
Hin1I	GRCGYC	3 cut(s)	318, 471, 498
Hin1II	CATG	8 cut(s)	358, 586, 799, 817, 1211, 1525, 1612, 1925
Hin6I	GCGC	8 cut(s)	282, 449, 451, 453, 498, 530, 940, 1053
HinP1I	GCGC	8 cut(s)	282, 449, 451, 453, 498, 530, 940, 1053
HincII	GTYRAC	2 cut(s)	1500, 1585
HindII	GTYRAC	2 cut(s)	1500, 1585
HindIII	AAGCTT	2 cut(s)	1134, 1280
HinfI	GANTC	6 cut(s)	610, 878, 1175, 1387, 1648, 1766
HphI	GGTGA	7 cut(s)	313, 367, 631, 1111, 1840, 1960, 1988
Hpy166II	GTNNAC	3 cut(s)	1162, 1500, 1585
Hpy188I	TCNGA	8 cut(s)	232, 615, 877, 1002, 1078, 1771, 1848, 1863
Hpy188III	TCNNGA	9 cut(s)	93, 252, 605, 662, 748, 1397, 1568, 1609, 1916
Hpy8I	GTNNAC	3 cut(s)	1162, 1500, 1585
Hpy99I	CGWCG	2 cut(s)	530, 599
HpyAV	CCTTC	8 cut(s)	42, 52, 81, 148, 1831, 1843, 1963, 1967
HpyCH4III	ACNGT	2 cut(s)	1048, 1380
HpyCH4IV	ACGT	1 cut(s)	399
HpyCH4V	TGCA	6 cut(s)	477, 1039, 1181, 1421, 1562, 1799
HpyF3I	CTNAG	3 cut(s)	876, 1464, 1747
HpySE526I	ACGT	1 cut(s)	399
Hsp92I	GRCGYC	3 cut(s)	318, 471, 498
Hsp92II	CATG	8 cut(s)	358, 586, 799, 817, 1211, 1525, 1612, 1925
HspAI	GCGC	8 cut(s)	282, 449, 451, 453, 498, 530, 940, 1053
KasI	GGCGCC	1 cut(s)	497
Kpn2I	TCCGGA	1 cut(s)	92
Ksp22I	TGATCA	1 cut(s)	1965
KspI	CCGCGG	2 cut(s)	1509, 1557
LmnI	GCTCC	6 cut(s)	347, 386, 537, 949, 1010, 1517
Lsp1109I	GCAGC	5 cut(s)	421, 1213, 1456, 1528, 1807
LweI	GCATC	5 cut(s)	162, 435, 486, 1456, 1561
MaeI	CTAG	4 cut(s)	681, 819, 924, 1143
MaeII	ACGT	1 cut(s)	399
MaeIII	GTNAC	4 cut(s)	319, 625, 1117, 1828
MbiI	CCGCTC	1 cut(s)	381
MboII	GAAGA	6 cut(s)	66, 421, 683, 1672, 1846, 1995
MfeI	CAATTG	1 cut(s)	781
MflI	RGATCY	4 cut(s)	128, 360, 632, 960
MhlI	GDGCHC	3 cut(s)	273, 1007, 1738
MlsI	TGGCCA	1 cut(s)	710
MluNI	TGGCCA	1 cut(s)	710
Mly113I	GGCGCC	1 cut(s)	498
MlyI	GAGTC	3 cut(s)	604, 887, 1169
MmeI	TCCRAC	3 cut(s)	864, 1032, 1875
Mox20I	TGGCCA	1 cut(s)	710
MroI	TCCGGA	1 cut(s)	92
MroXI	GAANNNNTTC	1 cut(s)	1368
MscI	TGGCCA	1 cut(s)	710
MseI	TTAA	3 cut(s)	1284, 1350, 1406
MslI	CAYNNNNRTG	1 cut(s)	1640
Msp20I	TGGCCA	1 cut(s)	710
MspA1I	CMGCKG	4 cut(s)	1508, 1556, 1820, 1944
MunI	CAATTG	1 cut(s)	781
Mva1269I	GAATGC	1 cut(s)	1041
MvaI	CCWGG	6 cut(s)	342, 368, 807, 851, 1622, 1881
MvnI	CGCG	5 cut(s)	451, 453, 530, 1508, 1556
NarI	GGCGCC	1 cut(s)	498
NciI	CCSGG	8 cut(s)	468, 495, 726, 727, 958, 1733, 1825, 1826
NcoI	CCATGG	2 cut(s)	354, 1521
NheI	GCTAGC	1 cut(s)	1142
NlaIII	CATG	8 cut(s)	358, 586, 799, 817, 1211, 1525, 1612, 1925
NmeAIII	GCCGAG	3 cut(s)	463, 1172, 1277
NmuCI	GTSAC	3 cut(s)	319, 1117, 1828
NspI	RCATGY	2 cut(s)	817, 1925
PagI	TCATGA	1 cut(s)	1608
PauI	GCGCGC	2 cut(s)	449, 451
PceI	AGGCCT	1 cut(s)	1486
PciI	ACATGT	1 cut(s)	813
PcsI	WCGNNNNNNNCGW	1 cut(s)	534
PctI	GAATGC	1 cut(s)	1041
PdmI	GAANNNNTTC	1 cut(s)	1368
PfeI	GAWTC	3 cut(s)	1387, 1648, 1766
PflMI	CCANNNNNTGG	1 cut(s)	1627
PfoI	TCCNGGA	2 cut(s)	340, 1620
PinAI	ACCGGT	1 cut(s)	1433
PleI	GAGTC	3 cut(s)	604, 886, 1169
PluTI	GGCGCC	1 cut(s)	501
PpsI	GAGTC	3 cut(s)	604, 886, 1169
PpuMI	RGGWCCY	1 cut(s)	571
PscI	ACATGT	1 cut(s)	813
PsiI	TTATAA	2 cut(s)	1427, 1473
Psp124BI	GAGCTC	1 cut(s)	1007
Psp5II	RGGWCCY	1 cut(s)	571
Psp6I	CCWGG	6 cut(s)	340, 366, 805, 849, 1620, 1879
PspFI	CCCAGC	1 cut(s)	1940
PspGI	CCWGG	6 cut(s)	340, 366, 805, 849, 1620, 1879
PspPPI	RGGWCCY	1 cut(s)	571
PstI	CTGCAG	1 cut(s)	1183
PsuI	RGATCY	4 cut(s)	128, 360, 632, 960
PteI	GCGCGC	2 cut(s)	449, 451
PvuII	CAGCTG	1 cut(s)	1820
RsaI	GTAC	3 cut(s)	976, 1889, 1908
RsaNI	GTAC	3 cut(s)	975, 1888, 1907
RseI	CAYNNNNRTG	1 cut(s)	1640
SacI	GAGCTC	1 cut(s)	1007
SacII	CCGCGG	2 cut(s)	1509, 1557
SaqAI	TTAA	3 cut(s)	1284, 1350, 1406
ScaI	AGTACT	1 cut(s)	976
SchI	GAGTC	3 cut(s)	604, 887, 1169
SduI	GDGCHC	3 cut(s)	273, 1007, 1738
SfaNI	GCATC	5 cut(s)	162, 435, 486, 1456, 1561
SfcI	CTRYAG	1 cut(s)	1179
SfoI	GGCGCC	1 cut(s)	499
Sfr303I	CCGCGG	2 cut(s)	1509, 1557
SgrAI	CRCCGGYG	1 cut(s)	1433
SgrBI	CCGCGG	2 cut(s)	1509, 1557
SinI	GGWCC	3 cut(s)	571, 853, 1883
SmaI	CCCGGG	2 cut(s)	727, 1826
SmiMI	CAYNNNNRTG	1 cut(s)	1640
SrfI	GCCCGGGC	1 cut(s)	727
SseBI	AGGCCT	1 cut(s)	1486
SspDI	GGCGCC	1 cut(s)	497
SspMI	CTAG	4 cut(s)	681, 819, 924, 1143
SstI	GAGCTC	1 cut(s)	1007
StuI	AGGCCT	1 cut(s)	1486
StyI	CCWWGG	4 cut(s)	313, 354, 680, 1521
TaaI	ACNGT	2 cut(s)	1048, 1380
TaiI	ACGT	1 cut(s)	402
TaqI	TCGA	5 cut(s)	306, 537, 1107, 1131, 1398
TatI	WGTACW	1 cut(s)	974
TfiI	GAWTC	3 cut(s)	1387, 1648, 1766
Tru1I	TTAA	3 cut(s)	1284, 1350, 1406
Tru9I	TTAA	3 cut(s)	1284, 1350, 1406
TscAI	CASTG	6 cut(s)	520, 1051, 1245, 1499, 1662, 1709
TseFI	GTSAC	3 cut(s)	319, 1117, 1828
TseI	GCWGC	5 cut(s)	434, 1201, 1444, 1541, 1820
Tsp45I	GTSAC	3 cut(s)	319, 1117, 1828
TspDTI	ATGAA	5 cut(s)	1265, 1416, 1597, 1628, 1779
TspGWI	ACGGA	3 cut(s)	376, 1329, 1875
TspMI	CCCGGG	2 cut(s)	725, 1824
TspRI	CASTG	6 cut(s)	520, 1051, 1245, 1499, 1662, 1709
Van91I	CCANNNNNTGG	1 cut(s)	1627
VpaK11BI	GGWCC	3 cut(s)	571, 853, 1883
XagI	CCTNNNNNAGG	1 cut(s)	296
XceI	RCATGY	2 cut(s)	817, 1925
XcmI	CCANNNNNNNNNTGG	2 cut(s)	847, 979
XmaI	CCCGGG	2 cut(s)	725, 1824
XmaJI	CCTAGG	1 cut(s)	680
XmnI	GAANNNNTTC	1 cut(s)	1368
XspI	CTAG	4 cut(s)	681, 819, 924, 1143
ZrmI	AGTACT	1 cut(s)	976

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.