Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0006113

Physical Location & Seq

Genomic Coordinates Reverse (-)

913474 .. 915468

Gene Structure 1995 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g00840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1995 bp

ATGTCAAAAATGGCGGCCATCTCTCCATCCCCTTCCTCCTCCTTCTCCAGACCCTCTCCTCCTCCTTTGCCTTCTTCCCAAAATACCATTTCCGGATTTACCCTACCCTTTTCCTCCATTCCTCACAGATCCACTCCCTTCCGCCCTCTCCGCATCTCCAATTCCCTCAAACCTAAACCCACACCCAAGCCGACCGCTGCCGCCGCCGCTACCGCCACTACCTCCTCCGACCCCCAAACTTTCACTTCCCGATATGCCCCTGACGAGCCCAGAAAAGGCGCTGATGTCCTCGTGGAGGCCCTCGAACGCCAAGGCGTCACCGATGTATTCGCCTATCCTGGAGGAGCTTCCATGGAGATCCACCAGGCCCTCACCCGCTCCTCCGTCATCCGCAACGTCCTCCCCCGCCACGAGCAAGGCGGCATCTTTGCTGCCGAGGGATATGCGCGCGCCTCCGGCCTCCCCGGCGTCTGCATCGCCACCTCCGGCCCCGGCGCCACCAACTTGGTCAGTGGCCTCGCCGACGCGCTCCTCGACAGCGTGCCGTTGGTGGCAATCACGGGACAGGTCCCCCGCCGCATGATCGGCACCGACGCATTTCAAGAGACCCCGATTGTAGAGGTAACGAGATCCATCACCAAGCACAATTATCTTGTTCTTGATGTAGAAGATATCCCAAGGATTGTCAGCGAGGCATTTTTCTTGGCCAGCTCGGGTCGGCCCGGGCCGGTTTTGATAGACATCCCGAAAGATATACAGCAACAGCTTGTGGTTCCCAATTGGAACCAACCCATGAGTTTGCCTGGCTACATGTCTAGGTTGCCCAAGTCCCCCTCCAACTCCCACCTGGACCAGATTGTTAGGTTGGTCTCTGAGTCCAAGAGGCCCGTTTTGTATGTTGGCGGCGGCTGTTTGAACTCTAGCGAGGAGCTGAGGCGCTTTGTGGAGCTTACCGGGATCCCGGTTGCCAGTACTTTGATGGGTCTCGGTGCCTTTCCGAGCTCCAACGAACTGTCCCTCCAAATGCTTGGAATGCACGGCACTGTGTGCGCTAATTATTCTGTGGATAAGTCTGACTTGTTGCTTGCCTTTGGGGTGAGGTTCGATGATCGTGTCACGGGCAAGCTCGAAGCTTTTGCTAGCCGAGCAAAGATTGTCCACATTGACATTGACTCTGCAGAGATTGGAAAGAACAAGCAGCCTCATGTCTCGGTTTGTGCGGATGTGAAATTGGCACTGGCAGGGATGAACCGGACATTGGAAAACAAAGGAAGCAAGCTTAAACTTGATTTCTCGGCTTGGAGGGAGGAGCTGAATGAGCAGAAAGTGAAGTATCCGTTGAGTTTTAAGACTTTTGGGGAAGCCATTCCTCCACAGTATGCAATTCAGGTTCTCGACGAATTAACTGAGGGGAATGCAATTATAAGCACCGGTGTTGGGCAGCATCAAATGTGGGCGGCTCAGTTTTACAAATACAAGAGGCCTCGCCAGTGGTTGACATCCGCGGGATTAGGTGCCATGGGTTTTGGATTGCCTGCTGCTATTGGGGCTGCGGTTGCAAATCCAGATGAAATTGTTGTTGACATTGATGGTGATGGAAGTTTCATGATGAATGTCCAGGAATTGGCAACCATAAGAGTGGAGAATCTGCCAGTGAAGATAATGCTGTTGAATAATCAGCATTTGGGAATGGTGGTTCAGTGGGAGGATCGGTTCTATAAGGCAAACCGGGCTCACACTTACTTAGGGAACCCGTCAAATGAATCGGAGATATTCCCTAATATGCTGAAGTTTGCAGAGGCTTGCGGGATACCAGCTGCCCGGGTGACGAGGAAGGAACATCTAAAGGAGGCAATTCAGAAAATGCTGGACACTCCTGGTCCGTACTTGTTGGATGTAATAGTACCCCATCAAGAGCATGTGTTGCCTATGATCCCCAGCGGCGGTGCCTTCAAAGATGTGATCACCGAAGGTGATGGAAGATCGTCTTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009099 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

665

Amino Acids

71.93

Weight (kDa)

6.94

Isoelectric Point (pI)

45.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	92 - 202	4e-36	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	283 - 414	3.3e-45	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	478 - 633	1.1e-51	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48560
fragaria_vesca	FvH4_7g06770
malus_domestica	MD06G1028700.v1.1 MD10G1013700.v1.1
prunus_persica	Prupe.5G043100_v2.0.a1 Prupe.8G001300_v2.0.a1 Prupe.8G001300_v2.0.a1
pyrus_communis	pycom06g02280 pycom10g00840
rosa_chinensis	RchiOBHm_Chr7g0208791
rosa_laevigata	RLG00000003195 RLG00000015583
rosa_multiflora	Rmu_co8493263.1_g000001 Rmu_sc0000278.1_g000002 Rmu_sc0008663.1_g000001 Rmu_sc0018750.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250260 Rroxscaffold_7G00217760
rosa_rugosa	Rorug05G0492000 Rorug07G0105800
rosa_samantha	Rh6AG001800 Rh7AG241000 Rh7BG235900 Rh7CG255600 Rh7DG247000
rosa_wichuraiana	Rw6G000240 Rw7G020440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1424
AccB1I	GGYRCC	5 cut(s)	494, 587, 989, 1514, 1946
AccB7I	CCANNNNNTGG	1 cut(s)	1624
AccBSI	CCGCTC	1 cut(s)	378
AccII	CGCG	4 cut(s)	448, 450, 527, 1505
AccIII	TCCGGA	1 cut(s)	92
AclWI	GGATC	7 cut(s)	123, 352, 624, 952, 965, 1716, 1927
AcoI	YGGCCR	2 cut(s)	15, 705
AcuI	CTGAAG	1 cut(s)	1808
AcyI	GRCGYC	3 cut(s)	315, 468, 495
AdeI	CACNNNGTG	1 cut(s)	1047
AfaI	GTAC	3 cut(s)	973, 1886, 1905
AfiI	CCNNNNNNNGG	6 cut(s)	275, 295, 1258, 1437, 1624, 1943
AflIII	ACRYGT	1 cut(s)	810
AgeI	ACCGGT	1 cut(s)	1430
AgsI	TTSAA	4 cut(s)	602, 916, 1672, 1954
AjnI	CCWGG	6 cut(s)	337, 363, 802, 846, 1617, 1876
AjuI	GAANNNNNNNTTGG	2 cut(s)	1241, 1273
AloI	GAACNNNNNNTCC	6 cut(s)	1002, 1034, 1680, 1697, 1712, 1729
Alw21I	GWGCWC	1 cut(s)	1004
Alw26I	GTCTC	4 cut(s)	599, 874, 989, 1213
AlwI	GGATC	7 cut(s)	123, 352, 624, 952, 965, 1716, 1927
Ama87I	CYCGRG	3 cut(s)	712, 722, 1821
Aor13HI	TCCGGA	1 cut(s)	92
ApeKI	GCWGC	7 cut(s)	197, 431, 1198, 1441, 1538, 1550, 1817
AsiGI	ACCGGT	1 cut(s)	1430
Asp700I	GAANNNNTTC	1 cut(s)	1365
AspLEI	GCGC	8 cut(s)	281, 448, 450, 452, 497, 529, 939, 1052
AspS9I	GGNCC	9 cut(s)	298, 367, 488, 568, 720, 725, 850, 885, 1880
AsuC2I	CCSGG	9 cut(s)	465, 492, 723, 724, 955, 962, 1730, 1822, 1823
AsuHPI	GGTGA	8 cut(s)	310, 364, 628, 1108, 1604, 1837, 1957, 1985
AsuNHI	GCTAGC	1 cut(s)	1139
AvaI	CYCGRG	3 cut(s)	712, 722, 1821
AvaII	GGWCC	3 cut(s)	568, 850, 1880
BalI	TGGCCA	1 cut(s)	707
BamHI	GGATCC	1 cut(s)	957
BanI	GGYRCC	5 cut(s)	494, 587, 989, 1514, 1946
BanII	GRGCYC	3 cut(s)	270, 1004, 1735
BauI	CACGAG	2 cut(s)	290, 410
Bbv12I	GWGCWC	1 cut(s)	1004
BbvCI	CCTCAGC	1 cut(s)	932
BbvI	GCAGC	7 cut(s)	184, 418, 1210, 1453, 1525, 1537, 1804
BccI	CCATC	8 cut(s)	26, 34, 641, 973, 1583, 1589, 1917, 1970
BceAI	ACGGC	2 cut(s)	529, 1054
BcgI	CGANNNNNNTGC	4 cut(s)	425, 459, 1118, 1152
BciT130I	CCWGG	6 cut(s)	339, 365, 804, 848, 1619, 1878
BciVI	GTATCC	2 cut(s)	1344, 1803
BclI	TGATCA	1 cut(s)	1962
BcnI	CCSGG	9 cut(s)	465, 492, 723, 724, 955, 962, 1730, 1822, 1823
BcoDI	GTCTC	4 cut(s)	599, 874, 989, 1213
BfaI	CTAG	3 cut(s)	816, 921, 1140
BfmI	CTRYAG	1 cut(s)	1176
BfoI	RGCGCY	3 cut(s)	282, 498, 940
BfuI	GTATCC	2 cut(s)	1344, 1803
BglI	GCCNNNNNGGC	1 cut(s)	465
BmcAI	AGTACT	1 cut(s)	973
Bme18I	GGWCC	3 cut(s)	568, 850, 1880
BmeT110I	CYCGRG	3 cut(s)	712, 722, 1821
BmgT120I	GGNCC	9 cut(s)	298, 367, 488, 568, 720, 725, 850, 885, 1880
BmsI	GCATC	4 cut(s)	162, 432, 483, 1453
BmtI	GCTAGC	1 cut(s)	1143
BpmI	CTGGAG	2 cut(s)	31, 360
Bpu10I	CCTNAGC	1 cut(s)	932
BpuMI	CCSGG	9 cut(s)	465, 492, 723, 724, 955, 962, 1730, 1822, 1823
BsaBI	GATNNNNATC	1 cut(s)	740
BsaHI	GRCGYC	3 cut(s)	315, 468, 495
BsaI	GGTCTC	3 cut(s)	599, 874, 989
BsaWI	WCCGGW	3 cut(s)	92, 1251, 1430
BsaXI	ACNNNNNCTCC	4 cut(s)	1002, 1032, 1697, 1727
Bsc4I	CCNNNNNNNGG	6 cut(s)	275, 295, 1258, 1437, 1624, 1943
Bse118I	RCCGGY	2 cut(s)	727, 1430
Bse1I	ACTGG	4 cut(s)	969, 1242, 1489, 1652
Bse8I	GATNNNNATC	1 cut(s)	740
BseAI	TCCGGA	1 cut(s)	92
BseBI	CCWGG	6 cut(s)	339, 365, 804, 848, 1619, 1878
BseGI	GGATG	7 cut(s)	26, 387, 741, 1228, 1251, 1499, 1900
BseJI	GATNNNNATC	1 cut(s)	740
BseLI	CCNNNNNNNGG	6 cut(s)	275, 295, 1258, 1437, 1624, 1943
BseMII	CTCAG	4 cut(s)	864, 923, 1398, 1475
BseNI	ACTGG	4 cut(s)	969, 1242, 1489, 1652
BsePI	GCGCGC	2 cut(s)	446, 448
BseRI	GAGGAG	9 cut(s)	28, 48, 51, 214, 357, 370, 521, 941, 1322
BseXI	GCAGC	7 cut(s)	184, 418, 1210, 1453, 1525, 1537, 1804
BseYI	CCCAGC	1 cut(s)	1937
Bsh1236I	CGCG	4 cut(s)	448, 450, 527, 1505
Bsh1285I	CGRYCG	1 cut(s)	195
BshNI	GGYRCC	5 cut(s)	494, 587, 989, 1514, 1946
BshTI	ACCGGT	1 cut(s)	1430
BsiEI	CGRYCG	1 cut(s)	195
BsiHKAI	GWGCWC	1 cut(s)	1004
BsiHKCI	CYCGRG	3 cut(s)	712, 722, 1821
BslFI	GGGAC	4 cut(s)	554, 576, 814, 1000
BslI	CCNNNNNNNGG	6 cut(s)	275, 295, 1258, 1437, 1624, 1943
BsmAI	GTCTC	4 cut(s)	599, 874, 989, 1213
BsmFI	GGGAC	4 cut(s)	554, 576, 814, 1000
BsmI	GAATGC	2 cut(s)	1038, 1420
Bso31I	GGTCTC	3 cut(s)	599, 874, 989
BsoBI	CYCGRG	3 cut(s)	712, 722, 1821
Bsp1286I	GDGCHC	3 cut(s)	270, 1004, 1735
Bsp13I	TCCGGA	1 cut(s)	92
Bsp19I	CCATGG	2 cut(s)	351, 1518
BspCNI	CTCAG	4 cut(s)	865, 924, 1399, 1474
BspEI	TCCGGA	1 cut(s)	92
BspFNI	CGCG	4 cut(s)	448, 450, 527, 1505
BspHI	TCATGA	1 cut(s)	1605
BspMAI	CTGCAG	1 cut(s)	1180
BspOI	GCTAGC	1 cut(s)	1143
BspPI	GGATC	7 cut(s)	123, 352, 624, 952, 965, 1716, 1927
BspT107I	GGYRCC	5 cut(s)	494, 587, 989, 1514, 1946
BspTNI	GGTCTC	3 cut(s)	599, 874, 989
BsrBI	CCGCTC	1 cut(s)	378
BsrFI	RCCGGY	2 cut(s)	727, 1430
BsrI	ACTGG	4 cut(s)	969, 1242, 1489, 1652
BssAI	RCCGGY	2 cut(s)	727, 1430
BssHII	GCGCGC	2 cut(s)	446, 448
BssNI	GRCGYC	3 cut(s)	315, 468, 495
BssSI	CACGAG	2 cut(s)	290, 410
BssT1I	CCWWGG	4 cut(s)	310, 351, 677, 1518
Bst2BI	CACGAG	2 cut(s)	290, 410
Bst2UI	CCWGG	6 cut(s)	339, 365, 804, 848, 1619, 1878
Bst4CI	ACNGT	3 cut(s)	1014, 1045, 1377
BstACI	GRCGYC	3 cut(s)	315, 468, 495
BstAPI	GCANNNNNTGC	2 cut(s)	1047, 1924
BstDEI	CTNAG	5 cut(s)	873, 932, 1407, 1461, 1744
BstDSI	CCRYGG	3 cut(s)	351, 1503, 1518
BstENI	CCTNNNNNAGG	1 cut(s)	293
BstF5I	GGATG	7 cut(s)	26, 387, 741, 1228, 1251, 1499, 1900
BstFNI	CGCG	4 cut(s)	448, 450, 527, 1505
BstH2I	RGCGCY	3 cut(s)	282, 498, 940
BstHHI	GCGC	8 cut(s)	281, 448, 450, 452, 497, 529, 939, 1052
BstMAI	GTCTC	4 cut(s)	599, 874, 989, 1213
BstMCI	CGRYCG	1 cut(s)	195
BstNI	CCWGG	6 cut(s)	339, 365, 804, 848, 1619, 1878
BstNSI	RCATGY	2 cut(s)	814, 1922
BstSFI	CTRYAG	1 cut(s)	1176
BstUI	CGCG	4 cut(s)	448, 450, 527, 1505
BstV1I	GCAGC	7 cut(s)	184, 418, 1210, 1453, 1525, 1537, 1804
BstX2I	RGATCY	4 cut(s)	128, 357, 629, 957
BstXI	CCANNNNNNTGG	3 cut(s)	505, 1028, 1639
BstYI	RGATCY	4 cut(s)	128, 357, 629, 957
BsuI	GTATCC	2 cut(s)	1344, 1803
BtgI	CCRYGG	3 cut(s)	351, 1503, 1518
BtgZI	GCGATG	1 cut(s)	460
BtsCI	GGATG	7 cut(s)	26, 387, 741, 1228, 1251, 1499, 1900
BtsIMutI	CAGTG	6 cut(s)	517, 1041, 1235, 1496, 1659, 1706
CciI	TCATGA	1 cut(s)	1605
CfoI	GCGC	8 cut(s)	281, 448, 450, 452, 497, 529, 939, 1052
Cfr10I	RCCGGY	2 cut(s)	727, 1430
Cfr13I	GGNCC	9 cut(s)	298, 367, 488, 568, 720, 725, 850, 885, 1880
Cfr42I	CCGCGG	1 cut(s)	1506
Cfr9I	CCCGGG	2 cut(s)	722, 1821
CseI	GACGC	4 cut(s)	304, 457, 533, 602
Csp6I	GTAC	3 cut(s)	972, 1885, 1904
CspAI	ACCGGT	1 cut(s)	1430
CviAII	CATG	8 cut(s)	352, 580, 793, 811, 1205, 1519, 1606, 1919
CviQI	GTAC	3 cut(s)	972, 1885, 1904
DdeI	CTNAG	5 cut(s)	873, 932, 1407, 1461, 1744
DinI	GGCGCC	1 cut(s)	496
DraIII	CACNNNGTG	1 cut(s)	1047
EaeI	YGGCCR	2 cut(s)	15, 705
EciI	GGCGGA	1 cut(s)	131
Ecl136II	GAGCTC	1 cut(s)	1002
Eco130I	CCWWGG	4 cut(s)	310, 351, 677, 1518
Eco147I	AGGCCT	1 cut(s)	1483
Eco24I	GRGCYC	3 cut(s)	270, 1004, 1735
Eco31I	GGTCTC	3 cut(s)	599, 874, 989
Eco32I	GATATC	1 cut(s)	673
Eco47I	GGWCC	3 cut(s)	568, 850, 1880
Eco53kI	GAGCTC	1 cut(s)	1002
Eco57I	CTGAAG	1 cut(s)	1808
Eco88I	CYCGRG	3 cut(s)	712, 722, 1821
EcoICRI	GAGCTC	1 cut(s)	1002
EcoNI	CCTNNNNNAGG	1 cut(s)	293
EcoO109I	RGGNCCY	3 cut(s)	298, 367, 568
EcoRII	CCWGG	6 cut(s)	337, 363, 802, 846, 1617, 1876
EcoRV	GATATC	1 cut(s)	673
EcoT14I	CCWWGG	4 cut(s)	310, 351, 677, 1518
EcoT38I	GRGCYC	3 cut(s)	270, 1004, 1735
EgeI	GGCGCC	1 cut(s)	496
EheI	GGCGCC	1 cut(s)	496
ErhI	CCWWGG	4 cut(s)	310, 351, 677, 1518
FaeI	CATG	8 cut(s)	355, 583, 796, 814, 1208, 1522, 1609, 1922
FalI	AAGNNNNNCTT	3 cut(s)	1061, 1093, 1972
FaqI	GGGAC	4 cut(s)	554, 576, 814, 1000
FatI	CATG	8 cut(s)	351, 579, 792, 810, 1204, 1518, 1605, 1918
FauI	CCCGC	5 cut(s)	383, 413, 581, 1498, 1799
FbaI	TGATCA	1 cut(s)	1962
FokI	GGATG	7 cut(s)	13, 374, 728, 1235, 1258, 1486, 1907
FriOI	GRGCYC	3 cut(s)	270, 1004, 1735
FspBI	CTAG	3 cut(s)	816, 921, 1140
GlaI	GCGC	8 cut(s)	280, 447, 449, 451, 496, 528, 938, 1051
GsaI	CCCAGC	1 cut(s)	1941
GsuI	CTGGAG	2 cut(s)	31, 360
HaeII	RGCGCY	3 cut(s)	282, 498, 940
HgaI	GACGC	4 cut(s)	304, 457, 533, 602
HhaI	GCGC	8 cut(s)	281, 448, 450, 452, 497, 529, 939, 1052
Hin1I	GRCGYC	3 cut(s)	315, 468, 495
Hin1II	CATG	8 cut(s)	355, 583, 796, 814, 1208, 1522, 1609, 1922
Hin6I	GCGC	8 cut(s)	279, 446, 448, 450, 495, 527, 937, 1050
HinP1I	GCGC	8 cut(s)	279, 446, 448, 450, 495, 527, 937, 1050
HincII	GTYRAC	2 cut(s)	1497, 1582
HindII	GTYRAC	2 cut(s)	1497, 1582
HindIII	AAGCTT	2 cut(s)	1131, 1277
HinfI	GANTC	4 cut(s)	875, 1172, 1645, 1763
HphI	GGTGA	8 cut(s)	310, 364, 628, 1108, 1604, 1837, 1957, 1985
Hpy166II	GTNNAC	3 cut(s)	1159, 1497, 1582
Hpy188I	TCNGA	6 cut(s)	229, 874, 999, 1075, 1768, 1860
Hpy8I	GTNNAC	3 cut(s)	1159, 1497, 1582
Hpy99I	CGWCG	3 cut(s)	527, 596, 1400
HpyAV	CCTTC	7 cut(s)	42, 52, 81, 148, 1828, 1960, 1964
HpyCH4III	ACNGT	3 cut(s)	1014, 1045, 1377
HpyCH4IV	ACGT	1 cut(s)	396
HpyCH4V	TGCA	7 cut(s)	474, 1036, 1178, 1382, 1418, 1559, 1796
HpyF3I	CTNAG	5 cut(s)	873, 932, 1407, 1461, 1744
HpySE526I	ACGT	1 cut(s)	396
Hsp92I	GRCGYC	3 cut(s)	315, 468, 495
Hsp92II	CATG	8 cut(s)	355, 583, 796, 814, 1208, 1522, 1609, 1922
HspAI	GCGC	8 cut(s)	279, 446, 448, 450, 495, 527, 937, 1050
KasI	GGCGCC	1 cut(s)	494
Kpn2I	TCCGGA	1 cut(s)	92
Ksp22I	TGATCA	1 cut(s)	1962
KspI	CCGCGG	1 cut(s)	1506
LmnI	GCTCC	7 cut(s)	344, 383, 534, 928, 946, 1007, 1309
Lsp1109I	GCAGC	7 cut(s)	184, 418, 1210, 1453, 1525, 1537, 1804
LweI	GCATC	4 cut(s)	162, 432, 483, 1453
MaeI	CTAG	3 cut(s)	816, 921, 1140
MaeII	ACGT	1 cut(s)	396
MaeIII	GTNAC	4 cut(s)	316, 622, 1114, 1825
MbiI	CCGCTC	1 cut(s)	378
MboII	GAAGA	4 cut(s)	66, 680, 1669, 1992
MfeI	CAATTG	1 cut(s)	778
MflI	RGATCY	4 cut(s)	128, 357, 629, 957
MhlI	GDGCHC	3 cut(s)	270, 1004, 1735
MlsI	TGGCCA	1 cut(s)	707
MluNI	TGGCCA	1 cut(s)	707
Mly113I	GGCGCC	1 cut(s)	495
MlyI	GAGTC	2 cut(s)	884, 1166
MmeI	TCCRAC	4 cut(s)	252, 861, 1029, 1872
Mox20I	TGGCCA	1 cut(s)	707
MroI	TCCGGA	1 cut(s)	92
MroXI	GAANNNNTTC	1 cut(s)	1365
MscI	TGGCCA	1 cut(s)	707
MseI	TTAA	3 cut(s)	1281, 1347, 1403
MslI	CAYNNNNRTG	1 cut(s)	1637
Msp20I	TGGCCA	1 cut(s)	707
MspA1I	CMGCKG	4 cut(s)	197, 1505, 1817, 1941
MunI	CAATTG	1 cut(s)	778
Mva1269I	GAATGC	2 cut(s)	1038, 1420
MvaI	CCWGG	6 cut(s)	339, 365, 804, 848, 1619, 1878
MvnI	CGCG	4 cut(s)	448, 450, 527, 1505
NarI	GGCGCC	1 cut(s)	495
NciI	CCSGG	9 cut(s)	465, 492, 723, 724, 955, 962, 1730, 1822, 1823
NcoI	CCATGG	2 cut(s)	351, 1518
NheI	GCTAGC	1 cut(s)	1139
NlaIII	CATG	8 cut(s)	355, 583, 796, 814, 1208, 1522, 1609, 1922
NmeAIII	GCCGAG	3 cut(s)	460, 1169, 1274
NmuCI	GTSAC	3 cut(s)	316, 1114, 1825
NspI	RCATGY	2 cut(s)	814, 1922
PagI	TCATGA	1 cut(s)	1605
PauI	GCGCGC	2 cut(s)	446, 448
PceI	AGGCCT	1 cut(s)	1483
PciI	ACATGT	1 cut(s)	810
PcsI	WCGNNNNNNNCGW	1 cut(s)	531
PctI	GAATGC	2 cut(s)	1038, 1420
PdmI	GAANNNNTTC	1 cut(s)	1365
PfeI	GAWTC	2 cut(s)	1645, 1763
PflMI	CCANNNNNTGG	1 cut(s)	1624
PfoI	TCCNGGA	2 cut(s)	337, 1617
PinAI	ACCGGT	1 cut(s)	1430
PleI	GAGTC	2 cut(s)	883, 1166
PluTI	GGCGCC	1 cut(s)	498
PpsI	GAGTC	2 cut(s)	883, 1166
PpuMI	RGGWCCY	1 cut(s)	568
PscI	ACATGT	1 cut(s)	810
PsiI	TTATAA	1 cut(s)	1424
Psp124BI	GAGCTC	1 cut(s)	1004
Psp5II	RGGWCCY	1 cut(s)	568
Psp6I	CCWGG	6 cut(s)	337, 363, 802, 846, 1617, 1876
PspFI	CCCAGC	1 cut(s)	1937
PspGI	CCWGG	6 cut(s)	337, 363, 802, 846, 1617, 1876
PspPI	GGNCC	9 cut(s)	298, 367, 488, 568, 720, 725, 850, 885, 1880
PspPPI	RGGWCCY	1 cut(s)	568
PstI	CTGCAG	1 cut(s)	1180
PsuI	RGATCY	4 cut(s)	128, 357, 629, 957
PteI	GCGCGC	2 cut(s)	446, 448
PvuII	CAGCTG	1 cut(s)	1817
RsaI	GTAC	3 cut(s)	973, 1886, 1905
RsaNI	GTAC	3 cut(s)	972, 1885, 1904
RseI	CAYNNNNRTG	1 cut(s)	1637
SacI	GAGCTC	1 cut(s)	1004
SacII	CCGCGG	1 cut(s)	1506
SaqAI	TTAA	3 cut(s)	1281, 1347, 1403
Sau96I	GGNCC	9 cut(s)	298, 367, 488, 568, 720, 725, 850, 885, 1880
ScaI	AGTACT	1 cut(s)	973
SchI	GAGTC	2 cut(s)	884, 1166
SduI	GDGCHC	3 cut(s)	270, 1004, 1735
SfaNI	GCATC	4 cut(s)	162, 432, 483, 1453
SfcI	CTRYAG	1 cut(s)	1176
SfoI	GGCGCC	1 cut(s)	496
Sfr303I	CCGCGG	1 cut(s)	1506
SgrAI	CRCCGGYG	1 cut(s)	1430
SgrBI	CCGCGG	1 cut(s)	1506
SinI	GGWCC	3 cut(s)	568, 850, 1880
SmaI	CCCGGG	2 cut(s)	724, 1823
SmiMI	CAYNNNNRTG	1 cut(s)	1637
SrfI	GCCCGGGC	1 cut(s)	724
SseBI	AGGCCT	1 cut(s)	1483
SspDI	GGCGCC	1 cut(s)	494
SspMI	CTAG	3 cut(s)	816, 921, 1140
SstI	GAGCTC	1 cut(s)	1004
StuI	AGGCCT	1 cut(s)	1483
StyI	CCWWGG	4 cut(s)	310, 351, 677, 1518
TaaI	ACNGT	3 cut(s)	1014, 1045, 1377
TaiI	ACGT	1 cut(s)	399
TaqI	TCGA	5 cut(s)	303, 534, 1104, 1128, 1395
TatI	WGTACW	1 cut(s)	971
TfiI	GAWTC	2 cut(s)	1645, 1763
Tru1I	TTAA	3 cut(s)	1281, 1347, 1403
Tru9I	TTAA	3 cut(s)	1281, 1347, 1403
TscAI	CASTG	6 cut(s)	517, 1048, 1242, 1496, 1659, 1706
TseFI	GTSAC	3 cut(s)	316, 1114, 1825
TseI	GCWGC	7 cut(s)	197, 431, 1198, 1441, 1538, 1550, 1817
Tsp45I	GTSAC	3 cut(s)	316, 1114, 1825
TspDTI	ATGAA	5 cut(s)	1262, 1584, 1594, 1625, 1776
TspGWI	ACGGA	3 cut(s)	373, 1326, 1872
TspMI	CCCGGG	2 cut(s)	722, 1821
TspRI	CASTG	6 cut(s)	517, 1048, 1242, 1496, 1659, 1706
Van91I	CCANNNNNTGG	1 cut(s)	1624
VpaK11BI	GGWCC	3 cut(s)	568, 850, 1880
XagI	CCTNNNNNAGG	1 cut(s)	293
XceI	RCATGY	2 cut(s)	814, 1922
XcmI	CCANNNNNNNNNTGG	2 cut(s)	844, 976
XmaI	CCCGGG	2 cut(s)	722, 1821
XmnI	GAANNNNTTC	1 cut(s)	1365
XspI	CTAG	3 cut(s)	816, 921, 1140
ZrmI	AGTACT	1 cut(s)	973

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.