Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0006113

Physical Location & Seq

Genomic Coordinates Reverse (-)

45491798 .. 45493783

Gene Structure 1986 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003195

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1986 bp

ATGGCGACCATAGCTCCACCCTCTTCCTCCTTCTCCAAACCCTCTCTTCCCACCAAATCCCCAAACCCACTTTCCCGATTTGCCCTCCCCTACTTCCCTTACCCCAATAAGCACACACCCTTCCGCCCTCTCCACATCTCCAACTCTCTCACCCCCAAAGCCGCCGCCACACCCGTCACCGCCACCACCACAACAACCACCGCTGCTCCTCCAGACCCAGCACCTCTAATTCCCCGATTTGCCCCCGACGAGCCCAGAAAAGGCGCCGATGTCCTCGTGGAGGCGCTCGAGCGCCAGGGCGTCACCACGGTCTTCGCCTACCCCGGCGGCGCGTCGATGGAGATCCACCAGGCCCTCACGCGCTCCACCACCATCCGCAACGTCCTCCCCCGCCACGAGCAGGGCGGCGTCTTCGCTGCCGAGGGCTACGCGCGCGCGTCGGGCCTTCCGGGCGTATGCATTGCCACCTCCGGCCCCGGTGCCACCAATCTGGTCAGCGGTCTCGCCGACGCGCTCATGGACAGCATTCCGATGGTGGCAATCACCGGACAAGTGCCCCGCCGGATGATCGGAACTGATGCTTTTCAGGAGACGCCGATTGTTGAGGTAACCAGATCCATCACCAAGCACAATTACCTTGTTCTTGATGTACAGGACATTCCTATAATTGTTAAAGAAGCGTTTTTCCTAGCTACATCGGGTAGACCCGGCCCTGTTTTGATTGATATACCCAAAGATGTGCAGCAACAGCTTGTGGTTCCGGATTGGGACCAACCCATTAGGTTAACTGGTTACATGTCTAGGTTGCCCAAAAGCCCCAGTGAGGTACATCTGGAGCAGATTGTGAGGTTGGTGTCGGAGTCGAAGAAACCGGTGTTGTATGTTGGTGGAGGGTGTTTGAACTCGAGTGAGGAGTTGAGGCGGTTTGTGGAGCTTACTGGGATCCCGGTTACTAGTACTTTGATGGGTCTCGGGACTTACCCGAGCTCCGATGAAGATGACTTGTCGCTGCAGATGCTTGGGATGCATGGAACTGTTTATGCTAACTATGCTGTGGACAAGGCCGACTTGTTGCTGGCTTTTGGTGTGAGGTTTGATGATCGTGTGACTGGGAAGCTGGAGGCATTTGCTAGCCGGGCTAAGATTGTGCACATTGATATTGATTCTGCAGAGATTGGGAAGAACAAGCAGCCTCATGTGTCTATTTGTGGTGATGTGAAACCAGCGTTGCAAGGGTTGAACAGGATATTGGAGGGGAAATTGGAGAAGCTTAAGCTTGATTTTTCAGCTTGGAGGGCGGAGCTGAAAGAGCAGAAATTGAAGTTTCCATTGGGTTACAAGACTTTTGGGGAGGCCATCCCTCCTCAGTATGCCATTCAGGTTCTTGATGAGTTAACTGAAGGGAAGGCTATTATAAGCACTGGTGTTGGGCAACATCAGATGTGGGCGGCTCAACATTACAAGTATCTTAGGCCCCGACAGTGGTTGACTTCGGGGGGTTTAGGAGCCATGGGTTTTGGATTGCCAGCTGCTATGGGGGCTGCGGCAGCAAATCCAGGTGCTATTGTTGTCGACATTGATGGTGATGGAAGTTTTATGATGAATGTCCAAGAATTGGCAACAATCAAGGTGGAGAATCTTCCTGTCAAAATTCTGCTGTTGAACAATCAACATTTGGGTATGGTTGTTCAGTGGGAGGATCGCTTCTATAAGGCAAACAGGGCTCACACATACTTGGGCAACCCAGCAAATGAGTCTGAGATCTTCCCAAATATGCTAAAGTTCGCAGAAGCTTGTGGGGTACCAGCTGCCCGTGTGACGAAGAAGGAAGACGTCAAAGCTGCAATCCAGAAAATGTTGGACACCCCTGGTCCATACTTGTTGGACGTTATTGTACCACATCAAGAGCATGTGTTGCCTATGATCCCAAGCGGTGGTGCTTTCAAAGATGTAATAACTGAGGGTGATGGAAGAAGAAGCTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009099 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

662

Amino Acids

71.64

Weight (kDa)

6.75

Isoelectric Point (pI)

41.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	87 - 197	5.6e-37	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	278 - 413	6.1e-45	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	475 - 630	8.1e-51	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48560
fragaria_vesca	FvH4_7g06770
malus_domestica	MD06G1028700.v1.1 MD10G1013700.v1.1
prunus_persica	Prupe.5G043100_v2.0.a1 Prupe.8G001300_v2.0.a1 Prupe.8G001300_v2.0.a1
pyrus_communis	pycom06g02280 pycom10g00840
rosa_chinensis	RchiOBHm_Chr7g0208791
rosa_laevigata	RLG00000003195 RLG00000015583
rosa_multiflora	Rmu_co8493263.1_g000001 Rmu_sc0000278.1_g000002 Rmu_sc0008663.1_g000001 Rmu_sc0018750.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250260 Rroxscaffold_7G00217760
rosa_rugosa	Rorug05G0492000 Rorug07G0105800
rosa_samantha	Rh6AG001800 Rh7AG241000 Rh7BG235900 Rh7CG255600 Rh7DG247000
rosa_wichuraiana	Rw6G000240 Rw7G020440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1415
AatII	GACGTC	1 cut(s)	1836
Acc65I	GGTACC	1 cut(s)	1801
AccB1I	GGYRCC	3 cut(s)	263, 479, 1801
AccB7I	CCANNNNNTGG	2 cut(s)	1615, 1934
AccI	GTMKAC	2 cut(s)	703, 1572
AccII	CGCG	7 cut(s)	332, 361, 431, 433, 435, 437, 512
AccIII	TCCGGA	1 cut(s)	760
AclWI	GGATC	6 cut(s)	337, 609, 937, 950, 1707, 1918
AcsI	RAATTY	1 cut(s)	1650
AcuI	CTGAAG	1 cut(s)	1419
AcyI	GRCGYC	5 cut(s)	264, 300, 408, 593, 1833
AfaI	GTAC	5 cut(s)	651, 828, 958, 1803, 1896
AfiI	CCNNNNNNNGG	7 cut(s)	260, 280, 400, 823, 1482, 1615, 1934
AflII	CTTAAG	1 cut(s)	1271
AflIII	ACRYGT	1 cut(s)	795
AgeI	ACCGGT	1 cut(s)	871
AgsI	TTSAA	5 cut(s)	901, 1240, 1321, 1663, 1945
AhlI	ACTAGT	1 cut(s)	953
AjnI	CCWGG	4 cut(s)	294, 348, 1555, 1867
AjuI	GAANNNNNNNTTGG	6 cut(s)	55, 87, 1232, 1264, 1313, 1345
Alw21I	GWGCWC	2 cut(s)	989, 1152
Alw26I	GTCTC	3 cut(s)	506, 584, 974
Alw44I	GTGCAC	1 cut(s)	1148
AlwI	GGATC	6 cut(s)	337, 609, 937, 950, 1707, 1918
Ama87I	CYCGRG	4 cut(s)	287, 904, 971, 982
Aor13HI	TCCGGA	1 cut(s)	760
AoxI	GGCC	7 cut(s)	351, 442, 472, 709, 1062, 1353, 1472
ApaLI	GTGCAC	1 cut(s)	1148
ApoI	RAATTY	1 cut(s)	1650
AsiGI	ACCGGT	1 cut(s)	871
Asp718I	GGTACC	1 cut(s)	1801
AspLEI	GCGC	9 cut(s)	266, 286, 294, 332, 363, 433, 435, 437, 514
AspS9I	GGNCC	7 cut(s)	352, 442, 473, 710, 769, 1473, 1871
AsuC2I	CCSGG	6 cut(s)	324, 450, 477, 708, 947, 1136
AsuHPI	GGTGA	8 cut(s)	142, 169, 295, 535, 613, 1223, 1595, 1976
AsuNHI	GCTAGC	1 cut(s)	1130
AvaI	CYCGRG	4 cut(s)	287, 904, 971, 982
AvaII	GGWCC	2 cut(s)	769, 1871
BaeGI	GKGCMC	2 cut(s)	558, 1152
BamHI	GGATCC	1 cut(s)	942
BanI	GGYRCC	3 cut(s)	263, 479, 1801
BanII	GRGCYC	3 cut(s)	255, 989, 1726
BauI	CACGAG	2 cut(s)	275, 395
BbsI	GAAGAC	3 cut(s)	304, 403, 1836
Bbv12I	GWGCWC	2 cut(s)	989, 1152
BccI	CCATC	9 cut(s)	331, 380, 526, 626, 958, 1364, 1574, 1580, 1961
BciT130I	CCWGG	4 cut(s)	296, 350, 1557, 1869
BcnI	CCSGG	6 cut(s)	324, 450, 477, 708, 947, 1136
BcoDI	GTCTC	3 cut(s)	506, 584, 974
BcuI	ACTAGT	1 cut(s)	953
BfaI	CTAG	4 cut(s)	689, 801, 954, 1131
BfmI	CTRYAG	2 cut(s)	1010, 1167
BfoI	RGCGCY	3 cut(s)	267, 287, 295
BfrI	CTTAAG	1 cut(s)	1271
BglI	GCCNNNNNGGC	1 cut(s)	450
BglII	AGATCT	1 cut(s)	1761
BmcAI	AGTACT	1 cut(s)	958
Bme18I	GGWCC	2 cut(s)	769, 1871
BmeT110I	CYCGRG	4 cut(s)	287, 904, 971, 982
BmgT120I	GGNCC	7 cut(s)	352, 442, 473, 710, 769, 1473, 1871
BmiI	GGNNCC	9 cut(s)	265, 475, 481, 759, 770, 944, 1475, 1507, 1803
BmrI	ACTGGG	3 cut(s)	813, 948, 1119
BmsI	GCATC	3 cut(s)	568, 1005, 1014
BmtI	GCTAGC	1 cut(s)	1134
BmuI	ACTGGG	3 cut(s)	813, 948, 1119
BpiI	GAAGAC	3 cut(s)	304, 403, 1836
BpmI	CTGGAG	3 cut(s)	195, 854, 1139
BpuMI	CCSGG	6 cut(s)	324, 450, 477, 708, 947, 1136
BsaBI	GATNNNNATC	1 cut(s)	341
BsaHI	GRCGYC	5 cut(s)	264, 300, 408, 593, 1833
BsaI	GGTCTC	2 cut(s)	506, 974
BsaJI	CCNNGG	7 cut(s)	295, 306, 322, 420, 475, 1509, 1867
BsaWI	WCCGGW	3 cut(s)	545, 760, 871
BsaXI	ACNNNNNCTCC	5 cut(s)	28, 190, 220, 971, 1001
Bsc4I	CCNNNNNNNGG	7 cut(s)	260, 280, 400, 823, 1482, 1615, 1934
Bse118I	RCCGGY	1 cut(s)	871
Bse1I	ACTGG	5 cut(s)	793, 819, 943, 1114, 1426
Bse3DI	GCAATG	1 cut(s)	459
Bse8I	GATNNNNATC	1 cut(s)	341
BseAI	TCCGGA	1 cut(s)	760
BseBI	CCWGG	4 cut(s)	296, 350, 1557, 1869
BseDI	CCNNGG	7 cut(s)	295, 306, 322, 420, 475, 1509, 1867
BseGI	GGATG	4 cut(s)	372, 570, 1029, 1356
BseJI	GATNNNNATC	1 cut(s)	341
BseLI	CCNNNNNNNGG	7 cut(s)	260, 280, 400, 823, 1482, 1615, 1934
BseMI	GCAATG	1 cut(s)	459
BseMII	CTCAG	3 cut(s)	1379, 1749, 1950
BseNI	ACTGG	5 cut(s)	793, 819, 943, 1114, 1426
BsePI	GCGCGC	2 cut(s)	431, 433
BseRI	GAGGAG	3 cut(s)	198, 926, 1353
BseSI	GKGCMC	2 cut(s)	558, 1152
BseYI	CCCAGC	2 cut(s)	217, 1744
BsgI	GTGCAG	1 cut(s)	761
Bsh1236I	CGCG	7 cut(s)	332, 361, 431, 433, 435, 437, 512
BshFI	GGCC	7 cut(s)	353, 444, 474, 711, 1064, 1355, 1474
BshNI	GGYRCC	3 cut(s)	263, 479, 1801
BshTI	ACCGGT	1 cut(s)	871
BsiHKAI	GWGCWC	2 cut(s)	989, 1152
BsiHKCI	CYCGRG	4 cut(s)	287, 904, 971, 982
BslFI	GGGAC	2 cut(s)	782, 988
BslI	CCNNNNNNNGG	7 cut(s)	260, 280, 400, 823, 1482, 1615, 1934
BsmAI	GTCTC	3 cut(s)	506, 584, 974
BsmBI	CGTCTC	1 cut(s)	584
BsmFI	GGGAC	2 cut(s)	782, 988
BsmI	GAATGC	1 cut(s)	525
BsnI	GGCC	7 cut(s)	353, 444, 474, 711, 1064, 1355, 1474
Bso31I	GGTCTC	2 cut(s)	506, 974
BsoBI	CYCGRG	4 cut(s)	287, 904, 971, 982
Bsp1286I	GDGCHC	5 cut(s)	255, 558, 989, 1152, 1726
Bsp13I	TCCGGA	1 cut(s)	760
Bsp1407I	TGTACA	1 cut(s)	649
Bsp143I	GATC	8 cut(s)	342, 567, 614, 942, 1099, 1699, 1761, 1923
Bsp19I	CCATGG	1 cut(s)	1509
BspANI	GGCC	7 cut(s)	353, 444, 474, 711, 1064, 1355, 1474
BspCNI	CTCAG	3 cut(s)	1378, 1750, 1951
BspEI	TCCGGA	1 cut(s)	760
BspFNI	CGCG	7 cut(s)	332, 361, 431, 433, 435, 437, 512
BspLI	GGNNCC	9 cut(s)	265, 475, 481, 759, 770, 944, 1475, 1507, 1803
BspMAI	CTGCAG	2 cut(s)	1014, 1171
BspOI	GCTAGC	1 cut(s)	1134
BspPI	GGATC	6 cut(s)	337, 609, 937, 950, 1707, 1918
BspT107I	GGYRCC	3 cut(s)	263, 479, 1801
BspTI	CTTAAG	1 cut(s)	1271
BspTNI	GGTCTC	2 cut(s)	506, 974
BsrDI	GCAATG	1 cut(s)	459
BsrFI	RCCGGY	1 cut(s)	871
BsrGI	TGTACA	1 cut(s)	649
BsrI	ACTGG	5 cut(s)	793, 819, 943, 1114, 1426
BssAI	RCCGGY	1 cut(s)	871
BssECI	CCNNGG	7 cut(s)	295, 306, 322, 420, 475, 1509, 1867
BssHII	GCGCGC	2 cut(s)	431, 433
BssMI	GATC	8 cut(s)	342, 567, 614, 942, 1099, 1699, 1761, 1923
BssNI	GRCGYC	5 cut(s)	264, 300, 408, 593, 1833
BssSI	CACGAG	2 cut(s)	275, 395
BssT1I	CCWWGG	1 cut(s)	1509
Bst2BI	CACGAG	2 cut(s)	275, 395
Bst2UI	CCWGG	4 cut(s)	296, 350, 1557, 1869
Bst4CI	ACNGT	3 cut(s)	310, 1036, 1482
Bst6I	CTCTTC	2 cut(s)	28, 51
BstACI	GRCGYC	5 cut(s)	264, 300, 408, 593, 1833
BstAFI	CTTAAG	1 cut(s)	1271
BstAPI	GCANNNNNTGC	1 cut(s)	1915
BstAUI	TGTACA	1 cut(s)	649
BstC8I	GCNNGC	5 cut(s)	433, 435, 1077, 1132, 1527
BstDEI	CTNAG	5 cut(s)	1140, 1365, 1469, 1758, 1959
BstDSI	CCRYGG	2 cut(s)	306, 1509
BstEII	GGTNACC	1 cut(s)	607
BstENI	CCTNNNNNAGG	1 cut(s)	278
BstF5I	GGATG	4 cut(s)	372, 570, 1029, 1356
BstFNI	CGCG	7 cut(s)	332, 361, 431, 433, 435, 437, 512
BstH2I	RGCGCY	3 cut(s)	267, 287, 295
BstHHI	GCGC	9 cut(s)	266, 286, 294, 332, 363, 433, 435, 437, 514
BstKTI	GATC	8 cut(s)	345, 570, 617, 945, 1102, 1702, 1764, 1926
BstMAI	GTCTC	3 cut(s)	506, 584, 974
BstMBI	GATC	8 cut(s)	342, 567, 614, 942, 1099, 1699, 1761, 1923
BstNI	CCWGG	4 cut(s)	296, 350, 1557, 1869
BstNSI	RCATGY	2 cut(s)	799, 1913
BstPI	GGTNACC	1 cut(s)	607
BstSFI	CTRYAG	2 cut(s)	1010, 1167
BstSLI	GKGCMC	2 cut(s)	558, 1152
BstUI	CGCG	7 cut(s)	332, 361, 431, 433, 435, 437, 512
BstV2I	GAAGAC	3 cut(s)	304, 403, 1836
BstX2I	RGATCY	4 cut(s)	342, 614, 942, 1761
BstXI	CCANNNNNNTGG	1 cut(s)	490
BstYI	RGATCY	4 cut(s)	342, 614, 942, 1761
BsuRI	GGCC	7 cut(s)	353, 444, 474, 711, 1064, 1355, 1474
BtgI	CCRYGG	2 cut(s)	306, 1509
BtsCI	GGATG	4 cut(s)	372, 570, 1029, 1356
BtsIMutI	CAGTG	4 cut(s)	826, 1419, 1487, 1697
Cac8I	GCNNGC	5 cut(s)	433, 435, 1077, 1132, 1527
CfoI	GCGC	9 cut(s)	266, 286, 294, 332, 363, 433, 435, 437, 514
Cfr10I	RCCGGY	1 cut(s)	871
Cfr13I	GGNCC	7 cut(s)	352, 442, 473, 710, 769, 1473, 1871
CseI	GACGC	6 cut(s)	289, 321, 397, 426, 518, 601
Csp6I	GTAC	5 cut(s)	650, 827, 957, 1802, 1895
CspAI	ACCGGT	1 cut(s)	871
CspCI	CAANNNNNGTGG	2 cut(s)	1611, 1646
CviAII	CATG	6 cut(s)	517, 796, 1028, 1196, 1510, 1910
CviQI	GTAC	5 cut(s)	650, 827, 957, 1802, 1895
DdeI	CTNAG	5 cut(s)	1140, 1365, 1469, 1758, 1959
DinI	GGCGCC	1 cut(s)	265
DpnI	GATC	8 cut(s)	344, 569, 616, 944, 1101, 1701, 1763, 1925
DpnII	GATC	8 cut(s)	342, 567, 614, 942, 1099, 1699, 1761, 1923
Eam1104I	CTCTTC	2 cut(s)	28, 51
EarI	CTCTTC	2 cut(s)	28, 51
EciI	GGCGGA	2 cut(s)	113, 1313
Ecl136II	GAGCTC	1 cut(s)	987
Eco130I	CCWWGG	1 cut(s)	1509
Eco24I	GRGCYC	3 cut(s)	255, 989, 1726
Eco31I	GGTCTC	2 cut(s)	506, 974
Eco47I	GGWCC	2 cut(s)	769, 1871
Eco53kI	GAGCTC	1 cut(s)	987
Eco57I	CTGAAG	1 cut(s)	1419
Eco88I	CYCGRG	4 cut(s)	287, 904, 971, 982
Eco91I	GGTNACC	1 cut(s)	607
EcoICRI	GAGCTC	1 cut(s)	987
EcoNI	CCTNNNNNAGG	1 cut(s)	278
EcoO109I	RGGNCCY	2 cut(s)	352, 1473
EcoO65I	GGTNACC	1 cut(s)	607
EcoRII	CCWGG	4 cut(s)	294, 348, 1555, 1867
EcoT14I	CCWWGG	1 cut(s)	1509
EcoT22I	ATGCAT	2 cut(s)	461, 1029
EcoT38I	GRGCYC	3 cut(s)	255, 989, 1726
EgeI	GGCGCC	1 cut(s)	265
EheI	GGCGCC	1 cut(s)	265
ErhI	CCWWGG	1 cut(s)	1509
Esp3I	CGTCTC	1 cut(s)	584
FaeI	CATG	6 cut(s)	520, 799, 1031, 1199, 1513, 1913
FalI	AAGNNNNNCTT	2 cut(s)	1052, 1084
FaqI	GGGAC	2 cut(s)	782, 988
FatI	CATG	6 cut(s)	516, 795, 1027, 1195, 1509, 1909
FauI	CCCGC	2 cut(s)	398, 566
FblI	GTMKAC	2 cut(s)	703, 1572
FokI	GGATG	4 cut(s)	359, 577, 1036, 1343
FriOI	GRGCYC	3 cut(s)	255, 989, 1726
FspBI	CTAG	4 cut(s)	689, 801, 954, 1131
GlaI	GCGC	9 cut(s)	265, 285, 293, 331, 362, 432, 434, 436, 513
GsaI	CCCAGC	2 cut(s)	221, 1748
GsuI	CTGGAG	3 cut(s)	195, 854, 1139
HaeII	RGCGCY	3 cut(s)	267, 287, 295
HaeIII	GGCC	7 cut(s)	353, 444, 474, 711, 1064, 1355, 1474
HgaI	GACGC	6 cut(s)	289, 321, 397, 426, 518, 601
HhaI	GCGC	9 cut(s)	266, 286, 294, 332, 363, 433, 435, 437, 514
Hin1I	GRCGYC	5 cut(s)	264, 300, 408, 593, 1833
Hin1II	CATG	6 cut(s)	520, 799, 1031, 1199, 1513, 1913
Hin6I	GCGC	9 cut(s)	264, 284, 292, 330, 361, 431, 433, 435, 512
HinP1I	GCGC	9 cut(s)	264, 284, 292, 330, 361, 431, 433, 435, 512
HincII	GTYRAC	4 cut(s)	786, 1395, 1488, 1573
HindII	GTYRAC	4 cut(s)	786, 1395, 1488, 1573
HindIII	AAGCTT	3 cut(s)	1268, 1274, 1791
HinfI	GANTC	4 cut(s)	860, 1163, 1636, 1754
HpaI	GTTAAC	2 cut(s)	786, 1395
HphI	GGTGA	8 cut(s)	142, 169, 295, 535, 613, 1223, 1595, 1976
Hpy166II	GTNNAC	7 cut(s)	704, 786, 1057, 1150, 1395, 1488, 1573
Hpy188I	TCNGA	6 cut(s)	531, 572, 859, 991, 1440, 1759
Hpy8I	GTNNAC	7 cut(s)	704, 786, 1057, 1150, 1395, 1488, 1573
Hpy99I	CGWCG	4 cut(s)	251, 337, 442, 512
HpyAV	CCTTC	6 cut(s)	40, 130, 455, 1394, 1399, 1819
HpyCH4III	ACNGT	3 cut(s)	310, 1036, 1482
HpyCH4IV	ACGT	3 cut(s)	381, 1833, 1887
HpyCH4V	TGCA	8 cut(s)	459, 742, 1012, 1027, 1150, 1169, 1231, 1844
HpyF3I	CTNAG	5 cut(s)	1140, 1365, 1469, 1758, 1959
HpySE526I	ACGT	3 cut(s)	381, 1833, 1887
Hsp92I	GRCGYC	5 cut(s)	264, 300, 408, 593, 1833
Hsp92II	CATG	6 cut(s)	520, 799, 1031, 1199, 1513, 1913
HspAI	GCGC	9 cut(s)	264, 284, 292, 330, 361, 431, 433, 435, 512
KasI	GGCGCC	1 cut(s)	263
Kpn2I	TCCGGA	1 cut(s)	760
KpnI	GGTACC	1 cut(s)	1805
KspAI	GTTAAC	2 cut(s)	786, 1395
Kzo9I	GATC	8 cut(s)	342, 567, 614, 942, 1099, 1699, 1761, 1923
LmnI	GCTCC	8 cut(s)	19, 211, 368, 835, 931, 992, 1300, 1505
LweI	GCATC	3 cut(s)	568, 1005, 1014
MaeI	CTAG	4 cut(s)	689, 801, 954, 1131
MaeII	ACGT	3 cut(s)	381, 1833, 1887
MaeIII	GTNAC	8 cut(s)	175, 301, 607, 791, 949, 1105, 1334, 1816
MalI	GATC	8 cut(s)	344, 569, 616, 944, 1101, 1701, 1763, 1925
MauBI	CGCGCGCG	2 cut(s)	431, 433
MboI	GATC	8 cut(s)	342, 567, 614, 942, 1099, 1699, 1761, 1923
MflI	RGATCY	4 cut(s)	342, 614, 942, 1761
MhlI	GDGCHC	5 cut(s)	255, 558, 989, 1152, 1726
MluCI	AATT	7 cut(s)	228, 631, 666, 1259, 1316, 1613, 1650
Mly113I	GGCGCC	1 cut(s)	264
MlyI	GAGTC	2 cut(s)	869, 1763
MmeI	TCCRAC	4 cut(s)	165, 837, 1839, 1863
Mph1103I	ATGCAT	2 cut(s)	461, 1029
MroI	TCCGGA	1 cut(s)	760
MseI	TTAA	4 cut(s)	672, 785, 1272, 1394
MslI	CAYNNNNRTG	1 cut(s)	530
MspA1I	CMGCKG	4 cut(s)	203, 498, 1529, 1808
MspCI	CTTAAG	1 cut(s)	1271
Mva1269I	GAATGC	1 cut(s)	525
MvaI	CCWGG	4 cut(s)	296, 350, 1557, 1869
MvnI	CGCG	7 cut(s)	332, 361, 431, 433, 435, 437, 512
NarI	GGCGCC	1 cut(s)	264
NciI	CCSGG	6 cut(s)	324, 450, 477, 708, 947, 1136
NcoI	CCATGG	1 cut(s)	1509
NdeII	GATC	8 cut(s)	342, 567, 614, 942, 1099, 1699, 1761, 1923
NheI	GCTAGC	1 cut(s)	1130
NlaIII	CATG	6 cut(s)	520, 799, 1031, 1199, 1513, 1913
NlaIV	GGNNCC	9 cut(s)	265, 475, 481, 759, 770, 944, 1475, 1507, 1803
NmeAIII	GCCGAG	1 cut(s)	445
NmuCI	GTSAC	4 cut(s)	175, 301, 1105, 1816
NsiI	ATGCAT	2 cut(s)	461, 1029
NspI	RCATGY	2 cut(s)	799, 1913
PaeR7I	CTCGAG	2 cut(s)	287, 904
PauI	GCGCGC	2 cut(s)	431, 433
PciI	ACATGT	1 cut(s)	795
PctI	GAATGC	1 cut(s)	525
PfeI	GAWTC	2 cut(s)	1163, 1636
PflMI	CCANNNNNTGG	2 cut(s)	1615, 1934
PinAI	ACCGGT	1 cut(s)	871
PleI	GAGTC	2 cut(s)	868, 1762
PluTI	GGCGCC	1 cut(s)	267
PpsI	GAGTC	2 cut(s)	868, 1762
PscI	ACATGT	1 cut(s)	795
PsiI	TTATAA	1 cut(s)	1415
Psp124BI	GAGCTC	1 cut(s)	989
Psp6I	CCWGG	4 cut(s)	294, 348, 1555, 1867
PspEI	GGTNACC	1 cut(s)	607
PspFI	CCCAGC	2 cut(s)	217, 1744
PspGI	CCWGG	4 cut(s)	294, 348, 1555, 1867
PspN4I	GGNNCC	9 cut(s)	265, 475, 481, 759, 770, 944, 1475, 1507, 1803
PspPI	GGNCC	7 cut(s)	352, 442, 473, 710, 769, 1473, 1871
PspXI	VCTCGAGB	2 cut(s)	287, 904
PstI	CTGCAG	2 cut(s)	1014, 1171
PsuI	RGATCY	4 cut(s)	342, 614, 942, 1761
PteI	GCGCGC	2 cut(s)	431, 433
PvuII	CAGCTG	2 cut(s)	1529, 1808
RsaI	GTAC	5 cut(s)	651, 828, 958, 1803, 1896
RsaNI	GTAC	5 cut(s)	650, 827, 957, 1802, 1895
RseI	CAYNNNNRTG	1 cut(s)	530
SacI	GAGCTC	1 cut(s)	989
SalI	GTCGAC	1 cut(s)	1571
SaqAI	TTAA	4 cut(s)	672, 785, 1272, 1394
Sau3AI	GATC	8 cut(s)	342, 567, 614, 942, 1099, 1699, 1761, 1923
Sau96I	GGNCC	7 cut(s)	352, 442, 473, 710, 769, 1473, 1871
ScaI	AGTACT	1 cut(s)	958
SchI	GAGTC	2 cut(s)	869, 1763
SduI	GDGCHC	5 cut(s)	255, 558, 989, 1152, 1726
SfaNI	GCATC	3 cut(s)	568, 1005, 1014
SfcI	CTRYAG	2 cut(s)	1010, 1167
SfoI	GGCGCC	1 cut(s)	265
Sfr274I	CTCGAG	2 cut(s)	287, 904
SinI	GGWCC	2 cut(s)	769, 1871
SlaI	CTCGAG	2 cut(s)	287, 904
SmiMI	CAYNNNNRTG	1 cut(s)	530
SmlI	CTYRAG	3 cut(s)	287, 904, 1271
SmoI	CTYRAG	3 cut(s)	287, 904, 1271
SpeI	ACTAGT	1 cut(s)	953
Sse9I	AATT	7 cut(s)	228, 631, 666, 1259, 1316, 1613, 1650
SspDI	GGCGCC	1 cut(s)	263
SspMI	CTAG	4 cut(s)	689, 801, 954, 1131
SstI	GAGCTC	1 cut(s)	989
StyI	CCWWGG	1 cut(s)	1509
TaaI	ACNGT	3 cut(s)	310, 1036, 1482
TaiI	ACGT	3 cut(s)	384, 1836, 1890
TaqI	TCGA	5 cut(s)	288, 335, 863, 905, 1572
TasI	AATT	7 cut(s)	228, 631, 666, 1259, 1316, 1613, 1650
TatI	WGTACW	2 cut(s)	649, 956
TauI	GCSGC	6 cut(s)	164, 167, 330, 408, 1451, 1547
TfiI	GAWTC	2 cut(s)	1163, 1636
Tru1I	TTAA	4 cut(s)	672, 785, 1272, 1394
Tru9I	TTAA	4 cut(s)	672, 785, 1272, 1394
TscAI	CASTG	4 cut(s)	826, 1426, 1487, 1697
TseFI	GTSAC	4 cut(s)	175, 301, 1105, 1816
Tsp45I	GTSAC	4 cut(s)	175, 301, 1105, 1816
TspDTI	ATGAA	2 cut(s)	1008, 1616
TspRI	CASTG	4 cut(s)	826, 1426, 1487, 1697
Van91I	CCANNNNNTGG	2 cut(s)	1615, 1934
Vha464I	CTTAAG	1 cut(s)	1271
VneI	GTGCAC	1 cut(s)	1148
VpaK11BI	GGWCC	2 cut(s)	769, 1871
XagI	CCTNNNNNAGG	1 cut(s)	278
XapI	RAATTY	1 cut(s)	1650
XceI	RCATGY	2 cut(s)	799, 1913
XhoI	CTCGAG	2 cut(s)	287, 904
XmiI	GTMKAC	2 cut(s)	703, 1572
XspI	CTAG	4 cut(s)	689, 801, 954, 1131
ZraI	GACGTC	1 cut(s)	1834
ZrmI	AGTACT	1 cut(s)	958
Zsp2I	ATGCAT	2 cut(s)	461, 1029

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.