Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0006113

Physical Location & Seq

Genomic Coordinates Reverse (-)

44069193 .. 44071463

Gene Structure 2271 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00250260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1983 bp

ATGGCGACCATAGCTCCACCCTCTTCCTCCTTCTCCAAACCCTCTCTTCCCACCAAATCCCCAAACCCACTTTCCCGATTTGCCCTCCCCTACTTCCCTTACCCCAACAAGCGCACACCCTTCCACCCTCTCCACATCTCCAACTCTCTCACCCCCAAAGCCGCCGCCACACCCGTCACGGCCACCACAACAACCACCGCTGCTCCTCCAGACCCAGCACCTCCAATTCCCCGATTTGCCCCCGACGAGCCCAGAAAAGGCGCCGATGTCCTCGTGGAGGCGCTCGAGCGCCAGGGCGTCACCACTGTGTTCGCCTACCCCGGCGGCGCGTCGCTGGAGATCCACCAGGCCCTCACGCGCTCCACCACCATCCGCAACGTCCTCCCCCGCCACGAGCAGGGCGGCGTCTTCGCCGCCGAGGGCTACGCGCGCGCGTCGGGCCTTCCGGGCGTATGCATTGCCACCTCCGGCCCCGGCGCGACCAATCTGGTGAGCGGTCTTGCCGACGCGCTCATGGACAGCATTCCGATGGTGGCAATCACCGGACAAGTGCCCCGCCGGATGATCGGAACTGATGCTTTTCAGGAGACGCCGATTGTTGAGGTAACCAGATCCATCACCAAGCACAATTACCTTGTTCTTGATGTAGAGGACATTCCTATAATTGTTAAAGAAGCTTTTTTCCTAGCTACATCGGGTAGACCCGGCCCGGTTTTGATTGATATACCCAAGGATGTGCAGCAACAGCTTGTGGTTCCGGATTGGGACCAACCCATTAGGTTAACTGGTTACATGTCTAGGTTGCCCAAAAGCCCCAGTGAGGTACATCTGGAGCAGATTGTGAGGTTGGTGTCGGAGTCGAAGAAACCGGTGTTGTATGTTGGTGGAGGGTGTTTGAACTCGAGTGAGGAGTTGAGGCGGTTCGTGGAGCTTACTGGGATCCCGGTTACTAGTACTTTGATGGGTCTCGGGACTTACCCGAGCTCCGATGAAGATGACTTGTCACTGCAGATGCTTGGGATGCATGGAACTGTTTATGCTAACTATGCTGTGGACAAGGCTGACTTGTTGCTGGCTTTTGGTGTGAGGTTTGATGATCGTGTGACTGGGAAGCTGGAGGCATTTGCTAGCCGGGCTAAGATTGTGCACATTGATATTGATTCTGCAGAGATTGGGAAGAACAAGCAGCCTCATGTGTCTATTTGTGGTGATGTGAAACCAGCGTTGCAAGGTTTGAACAGGATATTGGAGGGGAAATTGGAGAAGCTTAAGCTTGATTTTTCAGCTTGGAGGGCGGAGCTGAAAGAGCAGAAGTTGAAGTTTCCATTGGGTTACAAGACTTTTGCGGAGGCCATCCCTCCCCAGTATGCCATTCAGGTTCTTGATGAGTTAACTGAAGGGAAGGCTATTATAAGCACTGGTGTTGGGCAACATCAGATGTGGGCGGCTCAACATTACAAGTATCTTAGGCCCCGACAGTGGTTGACTTCAGGGGGTTTAGGAGCCATGGGTTTTGGATTGCCAGCTGCTATGGGGGCTGCAGCAGCAAATCCAGATGCTGTTGTTGTTGACATTGATGGTGATGGAAGTTTTATGATGAATGTCCAAGAATTGGCGACAATTAAGGTGGAGAATCTTCCCGTCAAGATTCTGCTGTTGAACAATCAGCATTTGGGTATGGTTGTTCAGTGGGAGGATCGCTTCTATAAGGCAAACAGGGCTCATACATACTTGGGCAACCCAGAAAATGAGACTGAGATCTTCCCAAATATGCTGAAGTTCGCAGAAGCTTGTGGGGTACCAGCTGCCCGTGTGACAAAGAAGGAAGACCTCAAAGCGGCAATCCAGAAAATGTTGGACACTCCTGGTCCATACTTGTTGGATGTTATAGTACCACATCAAGAGCATGTGTTGCCTATGATCCCAAGCGGTGGAGCTTTCAAAGATGTGATAACTGAGGGTGATGGAAGAAGAAGTTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009099 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

660

Amino Acids

71.65

Weight (kDa)

6.34

Isoelectric Point (pI)

41.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	86 - 196	8.3e-37	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	277 - 412	6e-45	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	474 - 629	1.4e-51	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48560
fragaria_vesca	FvH4_7g06770
malus_domestica	MD06G1028700.v1.1 MD10G1013700.v1.1
prunus_persica	Prupe.5G043100_v2.0.a1 Prupe.8G001300_v2.0.a1 Prupe.8G001300_v2.0.a1
pyrus_communis	pycom06g02280 pycom10g00840
rosa_chinensis	RchiOBHm_Chr7g0208791
rosa_laevigata	RLG00000003195 RLG00000015583
rosa_multiflora	Rmu_co8493263.1_g000001 Rmu_sc0000278.1_g000002 Rmu_sc0008663.1_g000001 Rmu_sc0018750.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250260 Rroxscaffold_7G00217760
rosa_rugosa	Rorug05G0492000 Rorug07G0105800
rosa_samantha	Rh6AG001800 Rh7AG241000 Rh7BG235900 Rh7CG255600 Rh7DG247000
rosa_wichuraiana	Rw6G000240 Rw7G020440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1412
Acc65I	GGTACC	1 cut(s)	1798
AccB1I	GGYRCC	2 cut(s)	260, 1798
AccB7I	CCANNNNNTGG	2 cut(s)	1612, 1931
AccBSI	CCGCTC	1 cut(s)	495
AccI	GTMKAC	1 cut(s)	700
AccII	CGCG	8 cut(s)	329, 358, 428, 430, 432, 434, 479, 509
AccIII	TCCGGA	1 cut(s)	757
AclWI	GGATC	6 cut(s)	334, 606, 934, 947, 1704, 1915
AcoI	YGGCCR	1 cut(s)	180
AcuI	CTGAAG	3 cut(s)	1416, 1473, 1796
AcyI	GRCGYC	4 cut(s)	261, 297, 405, 590
AfaI	GTAC	4 cut(s)	825, 955, 1800, 1893
AfiI	CCNNNNNNNGG	8 cut(s)	257, 277, 397, 820, 1479, 1612, 1837, 1931
AflII	CTTAAG	1 cut(s)	1268
AflIII	ACRYGT	1 cut(s)	792
AgeI	ACCGGT	1 cut(s)	868
AgsI	TTSAA	5 cut(s)	898, 1237, 1318, 1660, 1942
AhlI	ACTAGT	1 cut(s)	950
AjnI	CCWGG	3 cut(s)	291, 345, 1864
AjuI	GAANNNNNNNTTGG	6 cut(s)	55, 87, 1229, 1261, 1310, 1342
AleI	CACNNNNGTG	1 cut(s)	305
Alw21I	GWGCWC	2 cut(s)	986, 1149
Alw26I	GTCTC	3 cut(s)	581, 971, 1745
Alw44I	GTGCAC	1 cut(s)	1145
AlwI	GGATC	6 cut(s)	334, 606, 934, 947, 1704, 1915
AlwNI	CAGNNNCTG	1 cut(s)	1559
Ama87I	CYCGRG	4 cut(s)	284, 901, 968, 979
Aor13HI	TCCGGA	1 cut(s)	757
AoxI	GGCC	7 cut(s)	180, 348, 439, 469, 706, 1350, 1469
ApaLI	GTGCAC	1 cut(s)	1145
ApeKI	GCWGC	8 cut(s)	200, 739, 1186, 1526, 1538, 1541, 1544, 1805
AsiGI	ACCGGT	1 cut(s)	868
Asp718I	GGTACC	1 cut(s)	1798
AspS9I	GGNCC	7 cut(s)	349, 439, 470, 707, 766, 1470, 1868
AsuC2I	CCSGG	7 cut(s)	321, 447, 474, 705, 710, 944, 1133
AsuHPI	GGTGA	8 cut(s)	142, 292, 502, 532, 610, 1220, 1592, 1973
AsuNHI	GCTAGC	1 cut(s)	1127
AvaI	CYCGRG	4 cut(s)	284, 901, 968, 979
AvaII	GGWCC	2 cut(s)	766, 1868
BaeGI	GKGCMC	2 cut(s)	555, 1149
BamHI	GGATCC	1 cut(s)	939
BanI	GGYRCC	2 cut(s)	260, 1798
BanII	GRGCYC	3 cut(s)	252, 986, 1723
BauI	CACGAG	2 cut(s)	272, 392
BbsI	GAAGAC	2 cut(s)	400, 1833
Bbv12I	GWGCWC	2 cut(s)	986, 1149
BbvI	GCAGC	8 cut(s)	187, 751, 1198, 1513, 1525, 1553, 1556, 1792
BccI	CCATC	8 cut(s)	377, 523, 623, 955, 1361, 1571, 1577, 1958
BceAI	ACGGC	1 cut(s)	195
BciT130I	CCWGG	3 cut(s)	293, 347, 1866
BcnI	CCSGG	7 cut(s)	321, 447, 474, 705, 710, 944, 1133
BcoDI	GTCTC	3 cut(s)	581, 971, 1745
BcuI	ACTAGT	1 cut(s)	950
BfaI	CTAG	4 cut(s)	686, 798, 951, 1128
BfmI	CTRYAG	3 cut(s)	1007, 1164, 1539
BfoI	RGCGCY	3 cut(s)	264, 284, 292
BfrI	CTTAAG	1 cut(s)	1268
BglI	GCCNNNNNGGC	1 cut(s)	447
BglII	AGATCT	1 cut(s)	1758
BmcAI	AGTACT	1 cut(s)	955
Bme18I	GGWCC	2 cut(s)	766, 1868
BmeT110I	CYCGRG	4 cut(s)	284, 901, 968, 979
BmgT120I	GGNCC	7 cut(s)	349, 439, 470, 707, 766, 1470, 1868
BmiI	GGNNCC	8 cut(s)	262, 472, 756, 767, 941, 1472, 1504, 1800
BmrI	ACTGGG	4 cut(s)	810, 945, 1116, 1357
BmsI	GCATC	4 cut(s)	565, 1002, 1011, 1546
BmtI	GCTAGC	1 cut(s)	1131
BmuI	ACTGGG	4 cut(s)	810, 945, 1116, 1357
BpiI	GAAGAC	2 cut(s)	400, 1833
BpmI	CTGGAG	4 cut(s)	192, 356, 851, 1136
BpuMI	CCSGG	7 cut(s)	321, 447, 474, 705, 710, 944, 1133
BsaHI	GRCGYC	4 cut(s)	261, 297, 405, 590
BsaI	GGTCTC	1 cut(s)	971
BsaJI	CCNNGG	6 cut(s)	292, 319, 417, 472, 729, 1506
BsaWI	WCCGGW	3 cut(s)	542, 757, 868
BsaXI	ACNNNNNCTCC	5 cut(s)	28, 187, 217, 968, 998
Bsc4I	CCNNNNNNNGG	8 cut(s)	257, 277, 397, 820, 1479, 1612, 1837, 1931
Bse118I	RCCGGY	1 cut(s)	868
Bse1I	ACTGG	6 cut(s)	790, 816, 940, 1111, 1363, 1423
Bse3DI	GCAATG	1 cut(s)	456
BseAI	TCCGGA	1 cut(s)	757
BseBI	CCWGG	3 cut(s)	293, 347, 1866
BseDI	CCNNGG	6 cut(s)	292, 319, 417, 472, 729, 1506
BseGI	GGATG	6 cut(s)	369, 567, 739, 1026, 1353, 1888
BseLI	CCNNNNNNNGG	8 cut(s)	257, 277, 397, 820, 1479, 1612, 1837, 1931
BseMI	GCAATG	1 cut(s)	456
BseMII	CTCAG	2 cut(s)	1746, 1947
BseNI	ACTGG	6 cut(s)	790, 816, 940, 1111, 1363, 1423
BsePI	GCGCGC	2 cut(s)	428, 430
BseRI	GAGGAG	2 cut(s)	195, 923
BseSI	GKGCMC	2 cut(s)	555, 1149
BseXI	GCAGC	8 cut(s)	187, 751, 1198, 1513, 1525, 1553, 1556, 1792
BseYI	CCCAGC	1 cut(s)	214
BsgI	GTGCAG	1 cut(s)	758
Bsh1236I	CGCG	8 cut(s)	329, 358, 428, 430, 432, 434, 479, 509
BshFI	GGCC	7 cut(s)	182, 350, 441, 471, 708, 1352, 1471
BshNI	GGYRCC	2 cut(s)	260, 1798
BshTI	ACCGGT	1 cut(s)	868
BsiHKAI	GWGCWC	2 cut(s)	986, 1149
BsiHKCI	CYCGRG	4 cut(s)	284, 901, 968, 979
BslFI	GGGAC	2 cut(s)	779, 985
BslI	CCNNNNNNNGG	8 cut(s)	257, 277, 397, 820, 1479, 1612, 1837, 1931
BsmAI	GTCTC	3 cut(s)	581, 971, 1745
BsmBI	CGTCTC	1 cut(s)	581
BsmFI	GGGAC	2 cut(s)	779, 985
BsmI	GAATGC	1 cut(s)	522
BsnI	GGCC	7 cut(s)	182, 350, 441, 471, 708, 1352, 1471
Bso31I	GGTCTC	1 cut(s)	971
BsoBI	CYCGRG	4 cut(s)	284, 901, 968, 979
Bsp1286I	GDGCHC	5 cut(s)	252, 555, 986, 1149, 1723
Bsp13I	TCCGGA	1 cut(s)	757
Bsp143I	GATC	8 cut(s)	339, 564, 611, 939, 1096, 1696, 1758, 1920
Bsp19I	CCATGG	1 cut(s)	1506
BspANI	GGCC	7 cut(s)	182, 350, 441, 471, 708, 1352, 1471
BspCNI	CTCAG	2 cut(s)	1747, 1948
BspEI	TCCGGA	1 cut(s)	757
BspFNI	CGCG	8 cut(s)	329, 358, 428, 430, 432, 434, 479, 509
BspLI	GGNNCC	8 cut(s)	262, 472, 756, 767, 941, 1472, 1504, 1800
BspMAI	CTGCAG	3 cut(s)	1011, 1168, 1543
BspOI	GCTAGC	1 cut(s)	1131
BspPI	GGATC	6 cut(s)	334, 606, 934, 947, 1704, 1915
BspT107I	GGYRCC	2 cut(s)	260, 1798
BspTI	CTTAAG	1 cut(s)	1268
BspTNI	GGTCTC	1 cut(s)	971
BsrBI	CCGCTC	1 cut(s)	495
BsrDI	GCAATG	1 cut(s)	456
BsrFI	RCCGGY	1 cut(s)	868
BsrI	ACTGG	6 cut(s)	790, 816, 940, 1111, 1363, 1423
BssAI	RCCGGY	1 cut(s)	868
BssECI	CCNNGG	6 cut(s)	292, 319, 417, 472, 729, 1506
BssHII	GCGCGC	2 cut(s)	428, 430
BssMI	GATC	8 cut(s)	339, 564, 611, 939, 1096, 1696, 1758, 1920
BssNI	GRCGYC	4 cut(s)	261, 297, 405, 590
BssSI	CACGAG	2 cut(s)	272, 392
BssT1I	CCWWGG	2 cut(s)	729, 1506
Bst2BI	CACGAG	2 cut(s)	272, 392
Bst2UI	CCWGG	3 cut(s)	293, 347, 1866
Bst4CI	ACNGT	3 cut(s)	307, 1033, 1479
Bst6I	CTCTTC	2 cut(s)	28, 51
BstACI	GRCGYC	4 cut(s)	261, 297, 405, 590
BstAFI	CTTAAG	1 cut(s)	1268
BstAPI	GCANNNNNTGC	1 cut(s)	1912
BstC8I	GCNNGC	5 cut(s)	430, 432, 1074, 1129, 1524
BstDEI	CTNAG	4 cut(s)	1137, 1466, 1755, 1956
BstDSI	CCRYGG	1 cut(s)	1506
BstEII	GGTNACC	1 cut(s)	604
BstENI	CCTNNNNNAGG	1 cut(s)	275
BstF5I	GGATG	6 cut(s)	369, 567, 739, 1026, 1353, 1888
BstFNI	CGCG	8 cut(s)	329, 358, 428, 430, 432, 434, 479, 509
BstH2I	RGCGCY	3 cut(s)	264, 284, 292
BstKTI	GATC	8 cut(s)	342, 567, 614, 942, 1099, 1699, 1761, 1923
BstMAI	GTCTC	3 cut(s)	581, 971, 1745
BstMBI	GATC	8 cut(s)	339, 564, 611, 939, 1096, 1696, 1758, 1920
BstNI	CCWGG	3 cut(s)	293, 347, 1866
BstNSI	RCATGY	2 cut(s)	796, 1910
BstPI	GGTNACC	1 cut(s)	604
BstSFI	CTRYAG	3 cut(s)	1007, 1164, 1539
BstSLI	GKGCMC	2 cut(s)	555, 1149
BstUI	CGCG	8 cut(s)	329, 358, 428, 430, 432, 434, 479, 509
BstV1I	GCAGC	8 cut(s)	187, 751, 1198, 1513, 1525, 1553, 1556, 1792
BstV2I	GAAGAC	2 cut(s)	400, 1833
BstX2I	RGATCY	4 cut(s)	339, 611, 939, 1758
BstYI	RGATCY	4 cut(s)	339, 611, 939, 1758
BsuRI	GGCC	7 cut(s)	182, 350, 441, 471, 708, 1352, 1471
BtgI	CCRYGG	1 cut(s)	1506
BtsCI	GGATG	6 cut(s)	369, 567, 739, 1026, 1353, 1888
BtsI	GCAGTG	1 cut(s)	1004
BtsIMutI	CAGTG	6 cut(s)	303, 823, 1004, 1416, 1484, 1694
Cac8I	GCNNGC	5 cut(s)	430, 432, 1074, 1129, 1524
CaiI	CAGNNNCTG	1 cut(s)	1559
Cfr10I	RCCGGY	1 cut(s)	868
Cfr13I	GGNCC	7 cut(s)	349, 439, 470, 707, 766, 1470, 1868
CseI	GACGC	6 cut(s)	286, 318, 394, 423, 515, 598
Csp6I	GTAC	4 cut(s)	824, 954, 1799, 1892
CspAI	ACCGGT	1 cut(s)	868
CspCI	CAANNNNNGTGG	2 cut(s)	1608, 1643
CviAII	CATG	6 cut(s)	514, 793, 1025, 1193, 1507, 1907
CviQI	GTAC	4 cut(s)	824, 954, 1799, 1892
DdeI	CTNAG	4 cut(s)	1137, 1466, 1755, 1956
DinI	GGCGCC	1 cut(s)	262
DpnI	GATC	8 cut(s)	341, 566, 613, 941, 1098, 1698, 1760, 1922
DpnII	GATC	8 cut(s)	339, 564, 611, 939, 1096, 1696, 1758, 1920
EaeI	YGGCCR	1 cut(s)	180
Eam1104I	CTCTTC	2 cut(s)	28, 51
EarI	CTCTTC	2 cut(s)	28, 51
EciI	GGCGGA	1 cut(s)	1310
Ecl136II	GAGCTC	1 cut(s)	984
Eco130I	CCWWGG	2 cut(s)	729, 1506
Eco24I	GRGCYC	3 cut(s)	252, 986, 1723
Eco31I	GGTCTC	1 cut(s)	971
Eco47I	GGWCC	2 cut(s)	766, 1868
Eco53kI	GAGCTC	1 cut(s)	984
Eco57I	CTGAAG	3 cut(s)	1416, 1473, 1796
Eco88I	CYCGRG	4 cut(s)	284, 901, 968, 979
Eco91I	GGTNACC	1 cut(s)	604
EcoICRI	GAGCTC	1 cut(s)	984
EcoNI	CCTNNNNNAGG	1 cut(s)	275
EcoO109I	RGGNCCY	2 cut(s)	349, 1470
EcoO65I	GGTNACC	1 cut(s)	604
EcoRII	CCWGG	3 cut(s)	291, 345, 1864
EcoT14I	CCWWGG	2 cut(s)	729, 1506
EcoT22I	ATGCAT	2 cut(s)	458, 1026
EcoT38I	GRGCYC	3 cut(s)	252, 986, 1723
EgeI	GGCGCC	1 cut(s)	262
EheI	GGCGCC	1 cut(s)	262
ErhI	CCWWGG	2 cut(s)	729, 1506
Esp3I	CGTCTC	1 cut(s)	581
FaeI	CATG	6 cut(s)	517, 796, 1028, 1196, 1510, 1910
FalI	AAGNNNNNCTT	2 cut(s)	1049, 1081
FaqI	GGGAC	2 cut(s)	779, 985
FatI	CATG	6 cut(s)	513, 792, 1024, 1192, 1506, 1906
FauI	CCCGC	2 cut(s)	395, 563
FblI	GTMKAC	1 cut(s)	700
FokI	GGATG	6 cut(s)	356, 574, 746, 1033, 1340, 1895
FriOI	GRGCYC	3 cut(s)	252, 986, 1723
FspBI	CTAG	4 cut(s)	686, 798, 951, 1128
GsaI	CCCAGC	1 cut(s)	218
GsuI	CTGGAG	4 cut(s)	192, 356, 851, 1136
HaeII	RGCGCY	3 cut(s)	264, 284, 292
HaeIII	GGCC	7 cut(s)	182, 350, 441, 471, 708, 1352, 1471
HgaI	GACGC	6 cut(s)	286, 318, 394, 423, 515, 598
Hin1I	GRCGYC	4 cut(s)	261, 297, 405, 590
Hin1II	CATG	6 cut(s)	517, 796, 1028, 1196, 1510, 1910
HincII	GTYRAC	4 cut(s)	783, 1392, 1485, 1570
HindII	GTYRAC	4 cut(s)	783, 1392, 1485, 1570
HindIII	AAGCTT	4 cut(s)	675, 1265, 1271, 1788
HinfI	GANTC	4 cut(s)	857, 1160, 1633, 1648
HpaI	GTTAAC	2 cut(s)	783, 1392
HphI	GGTGA	8 cut(s)	142, 292, 502, 532, 610, 1220, 1592, 1973
Hpy166II	GTNNAC	7 cut(s)	701, 783, 1054, 1147, 1392, 1485, 1570
Hpy188I	TCNGA	5 cut(s)	528, 569, 856, 988, 1437
Hpy8I	GTNNAC	7 cut(s)	701, 783, 1054, 1147, 1392, 1485, 1570
Hpy99I	CGWCG	4 cut(s)	248, 334, 439, 509
HpyAV	CCTTC	6 cut(s)	40, 130, 452, 1391, 1396, 1816
HpyCH4III	ACNGT	3 cut(s)	307, 1033, 1479
HpyCH4IV	ACGT	1 cut(s)	378
HpyCH4V	TGCA	8 cut(s)	456, 739, 1009, 1024, 1147, 1166, 1228, 1541
HpyF3I	CTNAG	4 cut(s)	1137, 1466, 1755, 1956
HpySE526I	ACGT	1 cut(s)	378
Hsp92I	GRCGYC	4 cut(s)	261, 297, 405, 590
Hsp92II	CATG	6 cut(s)	517, 796, 1028, 1196, 1510, 1910
KasI	GGCGCC	1 cut(s)	260
Kpn2I	TCCGGA	1 cut(s)	757
KpnI	GGTACC	1 cut(s)	1802
KspAI	GTTAAC	2 cut(s)	783, 1392
Kzo9I	GATC	8 cut(s)	339, 564, 611, 939, 1096, 1696, 1758, 1920
LmnI	GCTCC	9 cut(s)	19, 208, 365, 832, 928, 989, 1297, 1502, 1934
Lsp1109I	GCAGC	8 cut(s)	187, 751, 1198, 1513, 1525, 1553, 1556, 1792
LweI	GCATC	4 cut(s)	565, 1002, 1011, 1546
MaeI	CTAG	4 cut(s)	686, 798, 951, 1128
MaeII	ACGT	1 cut(s)	378
MalI	GATC	8 cut(s)	341, 566, 613, 941, 1098, 1698, 1760, 1922
MauBI	CGCGCGCG	2 cut(s)	428, 430
MbiI	CCGCTC	1 cut(s)	495
MboI	GATC	8 cut(s)	339, 564, 611, 939, 1096, 1696, 1758, 1920
MflI	RGATCY	4 cut(s)	339, 611, 939, 1758
MhlI	GDGCHC	5 cut(s)	252, 555, 986, 1149, 1723
MluCI	AATT	6 cut(s)	225, 628, 663, 1256, 1610, 1620
Mly113I	GGCGCC	1 cut(s)	261
MlyI	GAGTC	1 cut(s)	866
MmeI	TCCRAC	4 cut(s)	165, 834, 1836, 1860
Mph1103I	ATGCAT	2 cut(s)	458, 1026
MroI	TCCGGA	1 cut(s)	757
MseI	TTAA	5 cut(s)	669, 782, 1269, 1391, 1623
MslI	CAYNNNNRTG	2 cut(s)	305, 527
MspA1I	CMGCKG	3 cut(s)	200, 1526, 1805
MspCI	CTTAAG	1 cut(s)	1268
Mva1269I	GAATGC	1 cut(s)	522
MvaI	CCWGG	3 cut(s)	293, 347, 1866
MvnI	CGCG	8 cut(s)	329, 358, 428, 430, 432, 434, 479, 509
NarI	GGCGCC	1 cut(s)	261
NciI	CCSGG	7 cut(s)	321, 447, 474, 705, 710, 944, 1133
NcoI	CCATGG	1 cut(s)	1506
NdeII	GATC	8 cut(s)	339, 564, 611, 939, 1096, 1696, 1758, 1920
NheI	GCTAGC	1 cut(s)	1127
NlaIII	CATG	6 cut(s)	517, 796, 1028, 1196, 1510, 1910
NlaIV	GGNNCC	8 cut(s)	262, 472, 756, 767, 941, 1472, 1504, 1800
NmeAIII	GCCGAG	1 cut(s)	442
NmuCI	GTSAC	5 cut(s)	175, 298, 1002, 1102, 1813
NsiI	ATGCAT	2 cut(s)	458, 1026
NspI	RCATGY	2 cut(s)	796, 1910
OliI	CACNNNNGTG	1 cut(s)	305
PaeR7I	CTCGAG	2 cut(s)	284, 901
PauI	GCGCGC	2 cut(s)	428, 430
PciI	ACATGT	1 cut(s)	792
PctI	GAATGC	1 cut(s)	522
PfeI	GAWTC	3 cut(s)	1160, 1633, 1648
PflMI	CCANNNNNTGG	2 cut(s)	1612, 1931
PinAI	ACCGGT	1 cut(s)	868
PleI	GAGTC	1 cut(s)	865
PluTI	GGCGCC	1 cut(s)	264
PpsI	GAGTC	1 cut(s)	865
PscI	ACATGT	1 cut(s)	792
PsiI	TTATAA	1 cut(s)	1412
Psp124BI	GAGCTC	1 cut(s)	986
Psp6I	CCWGG	3 cut(s)	291, 345, 1864
PspEI	GGTNACC	1 cut(s)	604
PspFI	CCCAGC	1 cut(s)	214
PspGI	CCWGG	3 cut(s)	291, 345, 1864
PspN4I	GGNNCC	8 cut(s)	262, 472, 756, 767, 941, 1472, 1504, 1800
PspPI	GGNCC	7 cut(s)	349, 439, 470, 707, 766, 1470, 1868
PspXI	VCTCGAGB	2 cut(s)	284, 901
PstI	CTGCAG	3 cut(s)	1011, 1168, 1543
PstNI	CAGNNNCTG	1 cut(s)	1559
PsuI	RGATCY	4 cut(s)	339, 611, 939, 1758
PteI	GCGCGC	2 cut(s)	428, 430
PvuII	CAGCTG	2 cut(s)	1526, 1805
RsaI	GTAC	4 cut(s)	825, 955, 1800, 1893
RsaNI	GTAC	4 cut(s)	824, 954, 1799, 1892
RseI	CAYNNNNRTG	2 cut(s)	305, 527
SacI	GAGCTC	1 cut(s)	986
SaqAI	TTAA	5 cut(s)	669, 782, 1269, 1391, 1623
Sau3AI	GATC	8 cut(s)	339, 564, 611, 939, 1096, 1696, 1758, 1920
Sau96I	GGNCC	7 cut(s)	349, 439, 470, 707, 766, 1470, 1868
ScaI	AGTACT	1 cut(s)	955
SchI	GAGTC	1 cut(s)	866
SduI	GDGCHC	5 cut(s)	252, 555, 986, 1149, 1723
SfaNI	GCATC	4 cut(s)	565, 1002, 1011, 1546
SfcI	CTRYAG	3 cut(s)	1007, 1164, 1539
SfoI	GGCGCC	1 cut(s)	262
Sfr274I	CTCGAG	2 cut(s)	284, 901
SinI	GGWCC	2 cut(s)	766, 1868
SlaI	CTCGAG	2 cut(s)	284, 901
SmiMI	CAYNNNNRTG	2 cut(s)	305, 527
SmlI	CTYRAG	3 cut(s)	284, 901, 1268
SmoI	CTYRAG	3 cut(s)	284, 901, 1268
SpeI	ACTAGT	1 cut(s)	950
Sse9I	AATT	6 cut(s)	225, 628, 663, 1256, 1610, 1620
SspDI	GGCGCC	1 cut(s)	260
SspMI	CTAG	4 cut(s)	686, 798, 951, 1128
SstI	GAGCTC	1 cut(s)	986
StyI	CCWWGG	2 cut(s)	729, 1506
TaaI	ACNGT	3 cut(s)	307, 1033, 1479
TaiI	ACGT	1 cut(s)	381
TaqI	TCGA	3 cut(s)	285, 860, 902
TasI	AATT	6 cut(s)	225, 628, 663, 1256, 1610, 1620
TatI	WGTACW	1 cut(s)	953
TauI	GCSGC	7 cut(s)	164, 167, 327, 405, 416, 1448, 1841
TfiI	GAWTC	3 cut(s)	1160, 1633, 1648
Tru1I	TTAA	5 cut(s)	669, 782, 1269, 1391, 1623
Tru9I	TTAA	5 cut(s)	669, 782, 1269, 1391, 1623
TscAI	CASTG	6 cut(s)	310, 823, 1011, 1423, 1484, 1694
TseFI	GTSAC	5 cut(s)	175, 298, 1002, 1102, 1813
TseI	GCWGC	8 cut(s)	200, 739, 1186, 1526, 1538, 1541, 1544, 1805
Tsp45I	GTSAC	5 cut(s)	175, 298, 1002, 1102, 1813
TspDTI	ATGAA	2 cut(s)	1005, 1613
TspRI	CASTG	6 cut(s)	310, 823, 1011, 1423, 1484, 1694
Van91I	CCANNNNNTGG	2 cut(s)	1612, 1931
Vha464I	CTTAAG	1 cut(s)	1268
VneI	GTGCAC	1 cut(s)	1145
VpaK11BI	GGWCC	2 cut(s)	766, 1868
XagI	CCTNNNNNAGG	1 cut(s)	275
XceI	RCATGY	2 cut(s)	796, 1910
XhoI	CTCGAG	2 cut(s)	284, 901
XmiI	GTMKAC	1 cut(s)	700
XspI	CTAG	4 cut(s)	686, 798, 951, 1128
ZrmI	AGTACT	1 cut(s)	955
Zsp2I	ATGCAT	2 cut(s)	458, 1026

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.