Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0006113

Physical Location & Seq

Genomic Coordinates Forward (+)

391275 .. 393224

Gene Structure 1950 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG001800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1950 bp

ATGGCGGCCATTGCTCCAGTCTCTTCCTCCTTGTCGAAATCGAAACCCTCGGCTTTCCCTTCTTCTTCTTCTTCTTCAACAAATGCCATTTCCAGATGTACTTTCCGCCCCTTCCCGCCCATTACCCACCGACCTCTGCACATTTCTAGTTCCCTCCCTCCTAAAGCCGCGACTGCCCCTTCGGACTCACAAAGTTTCACATCCCGATTTGCCCCTGACGAGCCCAGAAAAGGCTCCGATGTCCTCGTGGAGGCCCTCGAACGCCAAGGCGTCACCGACGTATTCGCCTACCCCGGAGGCGCATCCTTGGAGATCCATCAGGCCCTCACGCGCTCCTCCACCATCCGCAATGTTCTCCCCCGCCACGAGCAAGGTGGCATCTTCGCTGCTGAGGGCTACGCGCGCGCCTCGGGCCTTCCCGGCGTCTGTATTGCAACCTCGGGCCCTGGCGCTACCAACTTAGTCAGTGGCCTTGCTGACGCACTCCTTGACAGCGTTCCGGTCGTCGCTATCACCGGACAAGTCCCCCGCCGGATGATCGGTACCGACGCTTTTCAAGAGACCCCAATTGTAGAGGTAACCAGGTCCATTACAAAGCATAACTATCTTGTTCTTGACGTTGAAGAAATTCCTAGAATTGTTAGTGAGGCATTTTTCTTGGCCACCTCCGGCCGCCCCGGCCCAGTTTTGATAGATATACCAAAAGACATACAGCAACAGCTTGTCGTTCCGGATTGGAATCAACCCATTAGATTAGCGGGGTACATGTCTAGGCTGCCCAATCCTCCCAATGAGGCCCATTTGGAGCAGATTGTAAGGTTGGTTTCCGAATCCAAGAAGCCTGTGTTGTATGTTGGTGGAGGGTGCTTGAACTCGAGTGAGGAATTGAGGCGATTTGTGGAGTTTACAGGGATCCCTGTCGCAACTACCTTGATGGGTCTCGGGGCATATCCTAGCTCAGATGAGCTGTCACTCCAAATGTTGGGAATGCATGGGACAGTGCATGCTAATTATGCAGTGGATAAGTCTGACTTGTTGCTTGCATTTGGGGTTAGGTTTGATGACCGGGTTACAGGGAAGCTCGAAGCTTTTGCTAGCCGAGCTAAGATTGTTCACATTGATATTGATTCTGCAGAGATTGGAAAGAATAAGCAGCCTCATGTATCTGTTTGTGCAGATGTGAGATTGGCATTGGGAGGGATAAACAGGGTATTGGAGGGAAAAGGATACAAGTTCAAACTTGATTTCTCAGCCTGGAGGGAGGAGCTGAAAGAGCAGAAAATTAGTTATCCACTAAATTATAAGACATTTGGTGACGCCATTCCTCCTCAGTGTGCTATTCAGGTTCTTGATGAATTAACTGATGGGAATGCAATCATAAGCACCGGTGTCGGGCAGCATCAAATGTGGGCAGCTCAATTTTACAAATATAAGAGGCCTAGACAGTGGTTGACATCAGGAGGTTTAGGAGCTATGGGTTTTGGATTGCCTGCTGCTATTGGGGCAGCTGTGGCAAATCCAGATGCCATTGTTGTTGATATTGATGGTGATGGAAGCTTCATGATGAATGTTCAGGAATTAGCAACTATCAGGGTGGAAAATCTTCCCATTAAGATAATGCTGTTGAATAATCAGCATTTGGGTATGGTTGTTCAATGGGAGGATCGCTTCTATAAGGCGAATAGGGCTCACACTTATTTAGGGAACCCATTAAATGAGTCTGAGATATTCCCTAATATGCTCAAGTTCGCAGAAGCTTGTGGGATACCAGCTGCCCGTGTGACAAAGAAGGAACATCTTAAAGAGGCAATTCAGAAAATGCTGGACACTCCTGGTCCATACTTGTTGGATGTAGTAGTACCTCATCAAGAGCATGTCTTGCCCATGATTCCGAGTGGTGGTGCTTTTAAAGATGTGATAACTGAGGGTGATGGAAGATCATCTTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009099 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

649

Amino Acids

70.32

Weight (kDa)

6.37

Isoelectric Point (pI)

42.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	77 - 187	2.9e-36	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	268 - 398	3.1e-45	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	463 - 618	3.4e-52	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48560
fragaria_vesca	FvH4_7g06770
malus_domestica	MD06G1028700.v1.1 MD10G1013700.v1.1
prunus_persica	Prupe.5G043100_v2.0.a1 Prupe.8G001300_v2.0.a1 Prupe.8G001300_v2.0.a1
pyrus_communis	pycom06g02280 pycom10g00840
rosa_chinensis	RchiOBHm_Chr7g0208791
rosa_laevigata	RLG00000003195 RLG00000015583
rosa_multiflora	Rmu_co8493263.1_g000001 Rmu_sc0000278.1_g000002 Rmu_sc0008663.1_g000001 Rmu_sc0018750.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250260 Rroxscaffold_7G00217760
rosa_rugosa	Rorug05G0492000 Rorug07G0105800
rosa_samantha	Rh6AG001800 Rh7AG241000 Rh7BG235900 Rh7CG255600 Rh7DG247000
rosa_wichuraiana	Rw6G000240 Rw7G020440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1302
Acc65I	GGTACC	1 cut(s)	542
AccB1I	GGYRCC	1 cut(s)	542
AccB7I	CCANNNNNTGG	1 cut(s)	982
AccII	CGCG	5 cut(s)	170, 331, 401, 403, 405
AccIII	TCCGGA	1 cut(s)	730
AciI	CCGC	9 cut(s)	5, 106, 116, 168, 346, 361, 529, 673, 758
AclWI	GGATC	4 cut(s)	307, 907, 920, 1671
AcoI	YGGCCR	3 cut(s)	6, 660, 670
AcsI	RAATTY	1 cut(s)	627
AcyI	GRCGYC	3 cut(s)	270, 423, 1317
AfaI	GTAC	4 cut(s)	100, 544, 764, 1860
AfiI	CCNNNNNNNGG	5 cut(s)	230, 250, 982, 1392, 1898
AflIII	ACRYGT	1 cut(s)	765
AgeI	ACCGGT	1 cut(s)	1385
AgsI	TTSAA	7 cut(s)	78, 557, 623, 871, 1237, 1627, 1655
AjnI	CCWGG	4 cut(s)	445, 581, 1253, 1831
Alw26I	GTCTC	3 cut(s)	25, 554, 944
AlwI	GGATC	4 cut(s)	307, 907, 920, 1671
Ama87I	CYCGRG	4 cut(s)	409, 439, 874, 941
Aor13HI	TCCGGA	1 cut(s)	730
ApaI	GGGCCC	1 cut(s)	446
ApeKI	GCWGC	8 cut(s)	386, 775, 1153, 1396, 1412, 1493, 1505, 1772
ApoI	RAATTY	1 cut(s)	627
ArsI	GACNNNNNNTTYG	2 cut(s)	163, 195
AsiGI	ACCGGT	1 cut(s)	1385
Asp700I	GAANNNNTTC	2 cut(s)	627, 1602
Asp718I	GGTACC	1 cut(s)	542
AspLEI	GCGC	6 cut(s)	302, 333, 403, 405, 407, 452
AspS9I	GGNCC	9 cut(s)	253, 322, 412, 442, 443, 585, 680, 796, 1835
AsuC2I	CCSGG	4 cut(s)	294, 420, 678, 1067
AsuHPI	GGTGA	5 cut(s)	265, 505, 1325, 1559, 1940
AsuNHI	GCTAGC	1 cut(s)	1094
AvaI	CYCGRG	4 cut(s)	409, 439, 874, 941
AvaII	GGWCC	2 cut(s)	585, 1835
BaeGI	GKGCMC	1 cut(s)	446
BalI	TGGCCA	1 cut(s)	662
BamHI	GGATCC	1 cut(s)	912
BanI	GGYRCC	1 cut(s)	542
BanII	GRGCYC	3 cut(s)	225, 446, 1690
BauI	CACGAG	2 cut(s)	245, 365
BbvCI	CCTCAGC	1 cut(s)	390
BbvI	GCAGC	8 cut(s)	373, 762, 1165, 1408, 1424, 1480, 1517, 1759
BccI	CCATC	7 cut(s)	324, 350, 928, 1358, 1538, 1544, 1925
BcgI	CGANNNNNNTGC	4 cut(s)	1073, 1107, 1372, 1406
BciT130I	CCWGG	4 cut(s)	447, 583, 1255, 1833
BciVI	GTATCC	2 cut(s)	1220, 1758
BcnI	CCSGG	4 cut(s)	294, 420, 678, 1067
BcoDI	GTCTC	3 cut(s)	25, 554, 944
BfaI	CTAG	6 cut(s)	147, 633, 771, 954, 1095, 1440
BfmI	CTRYAG	1 cut(s)	1131
BfoI	RGCGCY	1 cut(s)	453
BfuI	GTATCC	2 cut(s)	1220, 1758
BglI	GCCNNNNNGGC	2 cut(s)	420, 678
Bme1390I	CCNGG	8 cut(s)	294, 420, 447, 583, 678, 1067, 1255, 1833
Bme18I	GGWCC	2 cut(s)	585, 1835
BmeT110I	CYCGRG	4 cut(s)	409, 439, 874, 941
BmgT120I	GGNCC	9 cut(s)	253, 322, 412, 442, 443, 585, 680, 796, 1835
BmiI	GGNNCC	5 cut(s)	235, 444, 544, 914, 1706
BmrFI	CCNGG	8 cut(s)	294, 420, 447, 583, 678, 1067, 1255, 1833
BmrI	ACTGGG	1 cut(s)	677
BmsI	GCATC	4 cut(s)	311, 387, 1408, 1513
BmtI	GCTAGC	1 cut(s)	1098
BmuI	ACTGGG	1 cut(s)	677
BpmI	CTGGAG	1 cut(s)	1276
Bpu10I	CCTNAGC	1 cut(s)	390
BpuEI	CTTGAG	1 cut(s)	1727
BpuMI	CCSGG	4 cut(s)	294, 420, 678, 1067
BsaBI	GATNNNNATC	1 cut(s)	738
BsaHI	GRCGYC	3 cut(s)	270, 423, 1317
BsaI	GGTCTC	2 cut(s)	554, 944
BsaJI	CCNNGG	8 cut(s)	48, 265, 292, 306, 408, 438, 445, 676
BsaWI	WCCGGW	4 cut(s)	499, 515, 730, 1385
Bsc4I	CCNNNNNNNGG	5 cut(s)	230, 250, 982, 1392, 1898
Bse118I	RCCGGY	1 cut(s)	1385
Bse1I	ACTGG	2 cut(s)	17, 683
Bse3DI	GCAATG	2 cut(s)	9, 355
Bse8I	GATNNNNATC	1 cut(s)	738
BseAI	TCCGGA	1 cut(s)	730
BseBI	CCWGG	4 cut(s)	447, 583, 1255, 1833
BseDI	CCNNGG	8 cut(s)	48, 265, 292, 306, 408, 438, 445, 676
BseGI	GGATG	5 cut(s)	200, 302, 342, 540, 1855
BseJI	GATNNNNATC	1 cut(s)	738
BseLI	CCNNNNNNNGG	5 cut(s)	230, 250, 982, 1392, 1898
BseMI	GCAATG	2 cut(s)	9, 355
BseMII	CTCAG	6 cut(s)	381, 972, 1263, 1343, 1713, 1914
BseNI	ACTGG	2 cut(s)	17, 683
BsePI	GCGCGC	2 cut(s)	401, 403
BseRI	GAGGAG	3 cut(s)	325, 1277, 1317
BseSI	GKGCMC	1 cut(s)	446
BseX3I	CGGCCG	1 cut(s)	670
BseXI	GCAGC	8 cut(s)	373, 762, 1165, 1408, 1424, 1480, 1517, 1759
BsgI	GTGCAG	2 cut(s)	122, 1194
Bsh1236I	CGCG	5 cut(s)	170, 331, 401, 403, 405
Bsh1285I	CGRYCG	2 cut(s)	504, 673
BshNI	GGYRCC	1 cut(s)	542
BshTI	ACCGGT	1 cut(s)	1385
BsiEI	CGRYCG	2 cut(s)	504, 673
BsiHKCI	CYCGRG	4 cut(s)	409, 439, 874, 941
BslFI	GGGAC	2 cut(s)	509, 1009
BslI	CCNNNNNNNGG	5 cut(s)	230, 250, 982, 1392, 1898
BsmAI	GTCTC	3 cut(s)	25, 554, 944
BsmFI	GGGAC	2 cut(s)	509, 1009
BsmI	GAATGC	2 cut(s)	993, 1375
Bso31I	GGTCTC	2 cut(s)	554, 944
BsoBI	CYCGRG	4 cut(s)	409, 439, 874, 941
Bsp120I	GGGCCC	1 cut(s)	442
Bsp1286I	GDGCHC	3 cut(s)	225, 446, 1690
Bsp13I	TCCGGA	1 cut(s)	730
Bsp143I	GATC	5 cut(s)	312, 537, 912, 1663, 1937
BspACI	CCGC	9 cut(s)	5, 106, 116, 168, 346, 361, 529, 673, 758
BspCNI	CTCAG	6 cut(s)	382, 971, 1262, 1342, 1714, 1915
BspEI	TCCGGA	1 cut(s)	730
BspFNI	CGCG	5 cut(s)	170, 331, 401, 403, 405
BspHI	TCATGA	1 cut(s)	1560
BspLI	GGNNCC	5 cut(s)	235, 444, 544, 914, 1706
BspMAI	CTGCAG	1 cut(s)	1135
BspOI	GCTAGC	1 cut(s)	1098
BspPI	GGATC	4 cut(s)	307, 907, 920, 1671
BspT107I	GGYRCC	1 cut(s)	542
BspTNI	GGTCTC	2 cut(s)	554, 944
BsrDI	GCAATG	2 cut(s)	9, 355
BsrFI	RCCGGY	1 cut(s)	1385
BsrI	ACTGG	2 cut(s)	17, 683
BssAI	RCCGGY	1 cut(s)	1385
BssECI	CCNNGG	8 cut(s)	48, 265, 292, 306, 408, 438, 445, 676
BssHII	GCGCGC	2 cut(s)	401, 403
BssMI	GATC	5 cut(s)	312, 537, 912, 1663, 1937
BssNI	GRCGYC	3 cut(s)	270, 423, 1317
BssSI	CACGAG	2 cut(s)	245, 365
BssT1I	CCWWGG	2 cut(s)	265, 306
Bst2BI	CACGAG	2 cut(s)	245, 365
Bst2UI	CCWGG	4 cut(s)	447, 583, 1255, 1833
Bst4CI	ACNGT	2 cut(s)	1000, 1446
Bst6I	CTCTTC	1 cut(s)	28
BstACI	GRCGYC	3 cut(s)	270, 423, 1317
BstAPI	GCANNNNNTGC	1 cut(s)	1879
BstC8I	GCNNGC	6 cut(s)	403, 405, 1005, 1041, 1096, 1491
BstDEI	CTNAG	8 cut(s)	390, 460, 958, 1104, 1249, 1329, 1722, 1923
BstEII	GGTNACC	1 cut(s)	577
BstENI	CCTNNNNNAGG	1 cut(s)	248
BstF5I	GGATG	5 cut(s)	200, 302, 342, 540, 1855
BstFNI	CGCG	5 cut(s)	170, 331, 401, 403, 405
BstH2I	RGCGCY	1 cut(s)	453
BstHHI	GCGC	6 cut(s)	302, 333, 403, 405, 407, 452
BstKTI	GATC	5 cut(s)	315, 540, 915, 1666, 1940
BstMAI	GTCTC	3 cut(s)	25, 554, 944
BstMBI	GATC	5 cut(s)	312, 537, 912, 1663, 1937
BstMCI	CGRYCG	2 cut(s)	504, 673
BstNI	CCWGG	4 cut(s)	447, 583, 1255, 1833
BstNSI	RCATGY	3 cut(s)	769, 1007, 1877
BstPI	GGTNACC	1 cut(s)	577
BstSCI	CCNGG	8 cut(s)	292, 418, 445, 581, 676, 1065, 1253, 1831
BstSFI	CTRYAG	1 cut(s)	1131
BstSLI	GKGCMC	1 cut(s)	446
BstUI	CGCG	5 cut(s)	170, 331, 401, 403, 405
BstV1I	GCAGC	8 cut(s)	373, 762, 1165, 1408, 1424, 1480, 1517, 1759
BstX2I	RGATCY	2 cut(s)	312, 912
BstYI	RGATCY	2 cut(s)	312, 912
BstZI	CGGCCG	1 cut(s)	670
BsuI	GTATCC	2 cut(s)	1220, 1758
BtsCI	GGATG	5 cut(s)	200, 302, 342, 540, 1855
BtsI	GCAGTG	1 cut(s)	1023
BtsIMutI	CAGTG	5 cut(s)	472, 1005, 1023, 1337, 1451
Cac8I	GCNNGC	6 cut(s)	403, 405, 1005, 1041, 1096, 1491
CciI	TCATGA	1 cut(s)	1560
CfoI	GCGC	6 cut(s)	302, 333, 403, 405, 407, 452
Cfr10I	RCCGGY	1 cut(s)	1385
Cfr13I	GGNCC	9 cut(s)	253, 322, 412, 442, 443, 585, 680, 796, 1835
CseI	GACGC	5 cut(s)	259, 412, 488, 557, 1325
CsiI	ACCWGGT	1 cut(s)	581
Csp6I	GTAC	4 cut(s)	99, 543, 763, 1859
CspAI	ACCGGT	1 cut(s)	1385
CviAII	CATG	7 cut(s)	766, 992, 1004, 1160, 1561, 1874, 1885
CviQI	GTAC	4 cut(s)	99, 543, 763, 1859
DdeI	CTNAG	8 cut(s)	390, 460, 958, 1104, 1249, 1329, 1722, 1923
DpnI	GATC	5 cut(s)	314, 539, 914, 1665, 1939
DpnII	GATC	5 cut(s)	312, 537, 912, 1663, 1937
DraI	TTTAAA	1 cut(s)	1909
EaeI	YGGCCR	3 cut(s)	6, 660, 670
EagI	CGGCCG	1 cut(s)	670
Eam1104I	CTCTTC	1 cut(s)	28
EarI	CTCTTC	1 cut(s)	28
EciI	GGCGGA	1 cut(s)	95
EclXI	CGGCCG	1 cut(s)	670
Eco130I	CCWWGG	2 cut(s)	265, 306
Eco147I	AGGCCT	1 cut(s)	1438
Eco24I	GRGCYC	3 cut(s)	225, 446, 1690
Eco31I	GGTCTC	2 cut(s)	554, 944
Eco47I	GGWCC	2 cut(s)	585, 1835
Eco52I	CGGCCG	1 cut(s)	670
Eco88I	CYCGRG	4 cut(s)	409, 439, 874, 941
Eco91I	GGTNACC	1 cut(s)	577
EcoNI	CCTNNNNNAGG	1 cut(s)	248
EcoO109I	RGGNCCY	3 cut(s)	253, 322, 443
EcoO65I	GGTNACC	1 cut(s)	577
EcoRII	CCWGG	4 cut(s)	445, 581, 1253, 1831
EcoT14I	CCWWGG	2 cut(s)	265, 306
EcoT22I	ATGCAT	1 cut(s)	993
EcoT38I	GRGCYC	3 cut(s)	225, 446, 1690
ErhI	CCWWGG	2 cut(s)	265, 306
FaeI	CATG	7 cut(s)	769, 995, 1007, 1163, 1564, 1877, 1888
FalI	AAGNNNNNCTT	3 cut(s)	1016, 1048, 1927
FaqI	GGGAC	2 cut(s)	509, 1009
FatI	CATG	7 cut(s)	765, 991, 1003, 1159, 1560, 1873, 1884
FauI	CCCGC	4 cut(s)	123, 368, 536, 751
FokI	GGATG	5 cut(s)	187, 289, 329, 547, 1862
FriOI	GRGCYC	3 cut(s)	225, 446, 1690
FspBI	CTAG	6 cut(s)	147, 633, 771, 954, 1095, 1440
GlaI	GCGC	6 cut(s)	301, 332, 402, 404, 406, 451
GsuI	CTGGAG	1 cut(s)	1276
HaeII	RGCGCY	1 cut(s)	453
HgaI	GACGC	5 cut(s)	259, 412, 488, 557, 1325
HhaI	GCGC	6 cut(s)	302, 333, 403, 405, 407, 452
Hin1I	GRCGYC	3 cut(s)	270, 423, 1317
Hin1II	CATG	7 cut(s)	769, 995, 1007, 1163, 1564, 1877, 1888
Hin6I	GCGC	6 cut(s)	300, 331, 401, 403, 405, 450
HinP1I	GCGC	6 cut(s)	300, 331, 401, 403, 405, 450
HincII	GTYRAC	1 cut(s)	1452
HindII	GTYRAC	1 cut(s)	1452
HindIII	AAGCTT	3 cut(s)	1086, 1555, 1755
HinfI	GANTC	6 cut(s)	185, 739, 830, 1127, 1718, 1888
HphI	GGTGA	5 cut(s)	265, 505, 1325, 1559, 1940
Hpy166II	GTNNAC	3 cut(s)	906, 1114, 1452
Hpy188I	TCNGA	8 cut(s)	184, 238, 829, 961, 1030, 1723, 1815, 1893
Hpy8I	GTNNAC	3 cut(s)	906, 1114, 1452
Hpy99I	CGWCG	3 cut(s)	281, 509, 551
HpyAV	CCTTC	5 cut(s)	69, 121, 189, 425, 1783
HpyCH4III	ACNGT	2 cut(s)	1000, 1446
HpyCH4IV	ACGT	2 cut(s)	279, 618
HpyCH4V	TGCA	9 cut(s)	139, 434, 991, 1003, 1016, 1043, 1133, 1175, 1373
HpyF3I	CTNAG	8 cut(s)	390, 460, 958, 1104, 1249, 1329, 1722, 1923
HpySE526I	ACGT	2 cut(s)	279, 618
Hsp92I	GRCGYC	3 cut(s)	270, 423, 1317
Hsp92II	CATG	7 cut(s)	769, 995, 1007, 1163, 1564, 1877, 1888
HspAI	GCGC	6 cut(s)	300, 331, 401, 403, 405, 450
Kpn2I	TCCGGA	1 cut(s)	730
KpnI	GGTACC	1 cut(s)	546
Kzo9I	GATC	5 cut(s)	312, 537, 912, 1663, 1937
LmnI	GCTCC	6 cut(s)	19, 239, 338, 805, 1264, 1469
Lsp1109I	GCAGC	8 cut(s)	373, 762, 1165, 1408, 1424, 1480, 1517, 1759
LweI	GCATC	4 cut(s)	311, 387, 1408, 1513
MabI	ACCWGGT	1 cut(s)	581
MaeI	CTAG	6 cut(s)	147, 633, 771, 954, 1095, 1440
MaeII	ACGT	2 cut(s)	279, 618
MaeIII	GTNAC	6 cut(s)	271, 577, 969, 1069, 1313, 1780
MalI	GATC	5 cut(s)	314, 539, 914, 1665, 1939
MauBI	CGCGCGCG	1 cut(s)	401
MboI	GATC	5 cut(s)	312, 537, 912, 1663, 1937
MfeI	CAATTG	1 cut(s)	567
MflI	RGATCY	2 cut(s)	312, 912
MhlI	GDGCHC	3 cut(s)	225, 446, 1690
MlsI	TGGCCA	1 cut(s)	662
MluNI	TGGCCA	1 cut(s)	662
MlyI	GAGTC	2 cut(s)	179, 1727
MmeI	TCCRAC	1 cut(s)	1827
Mox20I	TGGCCA	1 cut(s)	662
Mph1103I	ATGCAT	1 cut(s)	993
MroI	TCCGGA	1 cut(s)	730
MroXI	GAANNNNTTC	2 cut(s)	627, 1602
MscI	TGGCCA	1 cut(s)	662
MseI	TTAA	5 cut(s)	1358, 1611, 1712, 1800, 1908
Msp20I	TGGCCA	1 cut(s)	662
MspA1I	CMGCKG	2 cut(s)	1508, 1772
MspR9I	CCNGG	8 cut(s)	294, 420, 447, 583, 678, 1067, 1255, 1833
MunI	CAATTG	1 cut(s)	567
Mva1269I	GAATGC	2 cut(s)	993, 1375
MvaI	CCWGG	4 cut(s)	447, 583, 1255, 1833
MvnI	CGCG	5 cut(s)	170, 331, 401, 403, 405
NciI	CCSGG	4 cut(s)	294, 420, 678, 1067
NdeII	GATC	5 cut(s)	312, 537, 912, 1663, 1937
NheI	GCTAGC	1 cut(s)	1094
NlaIII	CATG	7 cut(s)	769, 995, 1007, 1163, 1564, 1877, 1888
NlaIV	GGNNCC	5 cut(s)	235, 444, 544, 914, 1706
NmeAIII	GCCGAG	2 cut(s)	29, 1124
NmuCI	GTSAC	4 cut(s)	271, 969, 1313, 1780
NsiI	ATGCAT	1 cut(s)	993
NspI	RCATGY	3 cut(s)	769, 1007, 1877
PaeI	GCATGC	1 cut(s)	1007
PaeR7I	CTCGAG	1 cut(s)	874
PagI	TCATGA	1 cut(s)	1560
PauI	GCGCGC	2 cut(s)	401, 403
PceI	AGGCCT	1 cut(s)	1438
PciI	ACATGT	1 cut(s)	765
PctI	GAATGC	2 cut(s)	993, 1375
PdmI	GAANNNNTTC	2 cut(s)	627, 1602
PfeI	GAWTC	4 cut(s)	739, 830, 1127, 1888
PflMI	CCANNNNNTGG	1 cut(s)	982
PinAI	ACCGGT	1 cut(s)	1385
PleI	GAGTC	2 cut(s)	179, 1726
PpsI	GAGTC	2 cut(s)	179, 1726
PscI	ACATGT	1 cut(s)	765
PsiI	TTATAA	1 cut(s)	1302
Psp6I	CCWGG	4 cut(s)	445, 581, 1253, 1831
PspEI	GGTNACC	1 cut(s)	577
PspGI	CCWGG	4 cut(s)	445, 581, 1253, 1831
PspN4I	GGNNCC	5 cut(s)	235, 444, 544, 914, 1706
PspOMI	GGGCCC	1 cut(s)	442
PspPI	GGNCC	9 cut(s)	253, 322, 412, 442, 443, 585, 680, 796, 1835
PspXI	VCTCGAGB	1 cut(s)	874
PstI	CTGCAG	1 cut(s)	1135
PsuI	RGATCY	2 cut(s)	312, 912
PteI	GCGCGC	2 cut(s)	401, 403
PvuII	CAGCTG	2 cut(s)	1508, 1772
RsaI	GTAC	4 cut(s)	100, 544, 764, 1860
RsaNI	GTAC	4 cut(s)	99, 543, 763, 1859
SaqAI	TTAA	5 cut(s)	1358, 1611, 1712, 1800, 1908
Sau3AI	GATC	5 cut(s)	312, 537, 912, 1663, 1937
Sau96I	GGNCC	9 cut(s)	253, 322, 412, 442, 443, 585, 680, 796, 1835
SchI	GAGTC	2 cut(s)	179, 1727
ScrFI	CCNGG	8 cut(s)	294, 420, 447, 583, 678, 1067, 1255, 1833
SduI	GDGCHC	3 cut(s)	225, 446, 1690
SexAI	ACCWGGT	1 cut(s)	581
SfaNI	GCATC	4 cut(s)	311, 387, 1408, 1513
SfcI	CTRYAG	1 cut(s)	1131
SfiI	GGCCNNNNNGGCC	1 cut(s)	678
Sfr274I	CTCGAG	1 cut(s)	874
SgrAI	CRCCGGYG	1 cut(s)	1385
SinI	GGWCC	2 cut(s)	585, 1835
SlaI	CTCGAG	1 cut(s)	874
SmlI	CTYRAG	2 cut(s)	874, 1742
SmoI	CTYRAG	2 cut(s)	874, 1742
SphI	GCATGC	1 cut(s)	1007
SseBI	AGGCCT	1 cut(s)	1438
SsiI	CCGC	9 cut(s)	5, 106, 116, 168, 346, 361, 529, 673, 758
SspMI	CTAG	6 cut(s)	147, 633, 771, 954, 1095, 1440
StuI	AGGCCT	1 cut(s)	1438
StyD4I	CCNGG	8 cut(s)	292, 418, 445, 581, 676, 1065, 1253, 1831
StyI	CCWWGG	2 cut(s)	265, 306
TaaI	ACNGT	2 cut(s)	1000, 1446
TaiI	ACGT	2 cut(s)	282, 621
TaqI	TCGA	5 cut(s)	35, 41, 258, 875, 1083
TatI	WGTACW	1 cut(s)	98
TauI	GCSGC	3 cut(s)	8, 170, 675
TfiI	GAWTC	4 cut(s)	739, 830, 1127, 1888
Tru1I	TTAA	5 cut(s)	1358, 1611, 1712, 1800, 1908
Tru9I	TTAA	5 cut(s)	1358, 1611, 1712, 1800, 1908
TscAI	CASTG	5 cut(s)	472, 1005, 1023, 1337, 1451
TseFI	GTSAC	4 cut(s)	271, 969, 1313, 1780
TseI	GCWGC	8 cut(s)	386, 775, 1153, 1396, 1412, 1493, 1505, 1772
Tsp45I	GTSAC	4 cut(s)	271, 969, 1313, 1780
TspDTI	ATGAA	3 cut(s)	1368, 1549, 1580
TspRI	CASTG	5 cut(s)	472, 1005, 1023, 1337, 1451
Van91I	CCANNNNNTGG	1 cut(s)	982
VpaK11BI	GGWCC	2 cut(s)	585, 1835
XagI	CCTNNNNNAGG	1 cut(s)	248
XapI	RAATTY	1 cut(s)	627
XceI	RCATGY	3 cut(s)	769, 1007, 1877
XcmI	CCANNNNNNNNNTGG	1 cut(s)	371
XhoI	CTCGAG	1 cut(s)	874
XmnI	GAANNNNTTC	2 cut(s)	627, 1602
XspI	CTAG	6 cut(s)	147, 633, 771, 954, 1095, 1440
Zsp2I	ATGCAT	1 cut(s)	993

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.