MD06G1104100.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003120
Physical Location & Seq
Reverse (-)
24218568 .. 24220572
2005 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1104100.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGATTCTGGTAAGAGCTGGGAGCAGAAGTCACTGGTCAATGAGATCATTGAAGGAATGGAGCTAGCAAAACAGTTGAGGCTATGTCTAAATGCAAAATCGTCAGCAGACACCAAGCAATTTTTAGTGCAGAGGATACTGTCGTCATATGAGAAGGCCCTTCTGATGCTGAAATGTGGCGGTTCGCCACAAAGTCCTATCAGAGCAATTGCTAGCGCGCCGGAGTCGCTAATGTCAGCCAATGAAGGTCCTTGTTGCGTTGACAATAACAGAAGTCTCCAGGATCATCAGGACCTGACGGCGGTCTCCAAGAAAAGAAAGGAAATGGCCAAATGGACAGAACACGTCACGAGAGTAATCTCTGAGAATGGGATTGAAGGACCCCATGAAGATGGCCACAGCTGGAGAAAATATGGGCAGAAAGACATCCTAGGAGCCAAACATCCAAGAAGCTATTACAGATGCACGTACCGGAACACGCAAAGTTGTTGGGCTACAAAGCAAGTGCAAAGATCAGATGAAGACCCCACCGTCTTCGAAATCACATACAAAGGGAAGCATACATGTTCTCATGGCGGCAGTTCAGTTCCACCGCCACCATCACCAGAAAAGCAAGAACGAAAACGACACAATCATAACAATACTTATCAACAACAGCAGTCTCAAGGAAACCAAATGAGCTTCCCAACTAATCTGAGAGTCAATACTGAGAACTTAGACGACAGAGAGAACACAGCATCTCCATTCTCTTTTACTTCATCTCCATTCGGAAGTATCAGCGATGACGCCTTCCTATCTTCGATGCTTGATGATCAAAGTCTTTTTGACCATTTCAATCAATCATTGCTGTCTCCAGCCGCAGGCGGATCAAACTATTACTTTGAGCTGCCCAGCCAGATGAGAAACATTGCAGGAAATGAGCAACGTTCGGAATCTGATATCATCTCAGCCAACAATTCGTCAACCAATTCTCCGATCCCGGACATGGATTTTCCACTGGAGCCAGTGGAACTCGACCCCTATTTCCCATTTGACTCCCCAGGATTTTTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 WRKY

Protein Analysis

352

Amino Acids

39.42

Weight (kDa)

5.72

Isoelectric Point (pI)

68.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 130 - 189 2.9e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1054
AasI GACNNNNNNGTC 1 cut(s) 530
AccII CGCG 1 cut(s) 218
AciI CCGC 6 cut(s) 180, 302, 576, 593, 858, 864
AclI AACGTT 1 cut(s) 925
AclWI GGATC 3 cut(s) 291, 874, 970
AcoI YGGCCR 2 cut(s) 327, 394
AcyI GRCGYC 1 cut(s) 786
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 3 cut(s) 301, 860, 985
AflIII ACRYGT 2 cut(s) 343, 563
AgsI TTSAA 3 cut(s) 53, 377, 835
AjiI CACGTC 1 cut(s) 346
AjnI CCWGG 2 cut(s) 279, 1039
AluBI AGCT 6 cut(s) 18, 64, 402, 453, 681, 886
AluI AGCT 6 cut(s) 18, 64, 402, 453, 681, 886
Alw26I GTCTC 4 cut(s) 281, 310, 666, 855
AlwI GGATC 3 cut(s) 291, 874, 970
AlwNI CAGNNNCTG 1 cut(s) 295
AoxI GGCC 3 cut(s) 156, 327, 394
ApeKI GCWGC 1 cut(s) 886
AspA2I CCTAGG 1 cut(s) 430
AspLEI GCGC 2 cut(s) 218, 220
AspS9I GGNCC 4 cut(s) 157, 248, 292, 380
AsuC2I CCSGG 1 cut(s) 980
AsuHPI GGTGA 1 cut(s) 594
AsuII TTCGAA 1 cut(s) 537
AsuNHI GCTAGC 2 cut(s) 64, 212
AvaII GGWCC 3 cut(s) 248, 292, 380
AvrII CCTAGG 1 cut(s) 430
BaeI ACNNNNGTAYC 2 cut(s) 128, 161
BalI TGGCCA 2 cut(s) 329, 396
BauI CACGAG 1 cut(s) 349
BbsI GAAGAC 2 cut(s) 526, 528
BbvI GCAGC 1 cut(s) 873
BccI CCATC 2 cut(s) 386, 607
BceAI ACGGC 1 cut(s) 315
BciT130I CCWGG 2 cut(s) 281, 1041
BciVI GTATCC 1 cut(s) 129
BclI TGATCA 1 cut(s) 811
BcnI CCSGG 1 cut(s) 980
BcoDI GTCTC 4 cut(s) 281, 310, 666, 855
BfaI CTAG 3 cut(s) 65, 213, 431
BfuI GTATCC 1 cut(s) 129
BisI GCNGC 3 cut(s) 577, 858, 887
BlnI CCTAGG 1 cut(s) 430
BlsI GCNGC 3 cut(s) 578, 859, 888
Bme1390I CCNGG 3 cut(s) 281, 980, 1041
Bme18I GGWCC 3 cut(s) 248, 292, 380
BmgBI CACGTC 1 cut(s) 346
BmgT120I GGNCC 4 cut(s) 157, 248, 292, 380
BmiI GGNNCC 3 cut(s) 382, 436, 1002
BmrFI CCNGG 3 cut(s) 281, 980, 1041
BmsI GCATC 4 cut(s) 156, 452, 746, 792
BmtI GCTAGC 2 cut(s) 68, 216
BoxI GACNNNNGTC 1 cut(s) 302
BpiI GAAGAC 2 cut(s) 526, 528
BpmI CTGGAG 4 cut(s) 263, 424, 837, 1019
Bpu14I TTCGAA 1 cut(s) 537
BpuEI CTTGAG 1 cut(s) 648
BpuMI CCSGG 1 cut(s) 980
BsaAI YACGTR 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 786
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 430, 1039
BsaWI WCCGGW 1 cut(s) 471
BsaXI ACNNNNNCTCC 8 cut(s) 14, 44, 724, 745, 754, 775, 955, 985
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 860, 985
Bse1I ACTGG 3 cut(s) 39, 1002, 1004
Bse3DI GCAATG 2 cut(s) 842, 906
BseBI CCWGG 2 cut(s) 281, 1041
BseDI CCNNGG 2 cut(s) 430, 1039
BseGI GGATG 2 cut(s) 426, 442
BseLI CCNNNNNNNGG 3 cut(s) 301, 860, 985
BseMI GCAATG 2 cut(s) 842, 906
BseMII CTCAG 4 cut(s) 354, 686, 699, 960
BseNI ACTGG 3 cut(s) 39, 1002, 1004
BsePI GCGCGC 1 cut(s) 216
BseXI GCAGC 1 cut(s) 873
BseYI CCCAGC 2 cut(s) 18, 890
BsgI GTGCAG 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 3 cut(s) 158, 329, 396
BsiSI CCGG 3 cut(s) 221, 472, 980
BslI CCNNNNNNNGG 3 cut(s) 301, 860, 985
BsmAI GTCTC 4 cut(s) 281, 310, 666, 855
BsnI GGCC 3 cut(s) 158, 329, 396
Bso31I GGTCTC 1 cut(s) 310
Bsp119I TTCGAA 1 cut(s) 537
Bsp143I GATC 6 cut(s) 45, 283, 512, 811, 866, 975
BspACI CCGC 6 cut(s) 180, 302, 576, 593, 858, 864
BspANI GGCC 3 cut(s) 158, 329, 396
BspCNI CTCAG 4 cut(s) 355, 687, 700, 959
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 3 cut(s) 382, 436, 1002
BspOI GCTAGC 2 cut(s) 68, 216
BspPI GGATC 3 cut(s) 291, 874, 970
BspT104I TTCGAA 1 cut(s) 537
BspTNI GGTCTC 1 cut(s) 310
BsrDI GCAATG 2 cut(s) 842, 906
BsrI ACTGG 3 cut(s) 39, 1002, 1004
BssECI CCNNGG 2 cut(s) 430, 1039
BssHII GCGCGC 1 cut(s) 216
BssMI GATC 6 cut(s) 45, 283, 512, 811, 866, 975
BssNI GRCGYC 1 cut(s) 786
BssSI CACGAG 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 430
Bst2BI CACGAG 1 cut(s) 349
Bst2UI CCWGG 2 cut(s) 281, 1041
Bst4CI ACNGT 3 cut(s) 75, 141, 532
BstACI GRCGYC 1 cut(s) 786
BstBAI YACGTR 1 cut(s) 468
BstBI TTCGAA 1 cut(s) 537
BstC8I GCNNGC 4 cut(s) 66, 214, 218, 862
BstDEI CTNAG 5 cut(s) 363, 695, 708, 715, 946
BstF5I GGATG 2 cut(s) 426, 442
BstFNI CGCG 1 cut(s) 218
BstHHI GCGC 2 cut(s) 218, 220
BstKTI GATC 6 cut(s) 48, 286, 515, 814, 869, 978
BstMAI GTCTC 4 cut(s) 281, 310, 666, 855
BstMBI GATC 6 cut(s) 45, 283, 512, 811, 866, 975
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 2 cut(s) 281, 1041
BstNSI RCATGY 1 cut(s) 567
BstPAI GACNNNNGTC 1 cut(s) 302
BstSCI CCNGG 3 cut(s) 279, 978, 1039
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 873
BstV2I GAAGAC 2 cut(s) 526, 528
BstXI CCANNNNNNTGG 1 cut(s) 392
BsuI GTATCC 1 cut(s) 129
BsuRI GGCC 3 cut(s) 158, 329, 396
BtgZI GCGATG 1 cut(s) 795
BtrI CACGTC 1 cut(s) 346
BtsCI GGATG 2 cut(s) 426, 442
BtsIMutI CAGTG 3 cut(s) 32, 995, 1011
Cac8I GCNNGC 4 cut(s) 66, 214, 218, 862
CaiI CAGNNNCTG 1 cut(s) 295
CfoI GCGC 2 cut(s) 218, 220
Cfr13I GGNCC 4 cut(s) 157, 248, 292, 380
CseI GACGC 1 cut(s) 794
Csp6I GTAC 1 cut(s) 469
CviAII CATG 4 cut(s) 386, 564, 572, 985
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 5 cut(s) 363, 695, 708, 715, 946
DpnI GATC 6 cut(s) 47, 285, 514, 813, 868, 977
DpnII GATC 6 cut(s) 45, 283, 512, 811, 866, 975
DrdI GACNNNNNNGTC 1 cut(s) 530
DseDI GACNNNNNNGTC 1 cut(s) 530
EaeI YGGCCR 2 cut(s) 327, 394
EciI GGCGGA 1 cut(s) 879
Eco130I CCWWGG 1 cut(s) 430
Eco31I GGTCTC 1 cut(s) 310
Eco32I GATATC 1 cut(s) 940
Eco47I GGWCC 3 cut(s) 248, 292, 380
EcoO109I RGGNCCY 4 cut(s) 157, 248, 292, 380
EcoRII CCWGG 2 cut(s) 279, 1039
EcoRV GATATC 1 cut(s) 940
EcoT14I CCWWGG 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 430
FaeI CATG 4 cut(s) 389, 567, 575, 988
FatI CATG 4 cut(s) 385, 563, 571, 984
FauNDI CATATG 1 cut(s) 148
FbaI TGATCA 1 cut(s) 811
Fnu4HI GCNGC 3 cut(s) 577, 858, 887
FokI GGATG 2 cut(s) 413, 429
Fsp4HI GCNGC 3 cut(s) 577, 858, 887
FspBI CTAG 3 cut(s) 65, 213, 431
GlaI GCGC 2 cut(s) 217, 219
GluI GCNGC 3 cut(s) 577, 858, 887
GsaI CCCAGC 2 cut(s) 22, 894
GsuI CTGGAG 4 cut(s) 263, 424, 837, 1019
HaeIII GGCC 3 cut(s) 158, 329, 396
HapII CCGG 3 cut(s) 221, 472, 980
HgaI GACGC 1 cut(s) 794
HhaI GCGC 2 cut(s) 218, 220
Hin1I GRCGYC 1 cut(s) 786
Hin1II CATG 4 cut(s) 389, 567, 575, 988
Hin6I GCGC 2 cut(s) 216, 218
HinP1I GCGC 2 cut(s) 216, 218
HincII GTYRAC 2 cut(s) 262, 963
HindII GTYRAC 2 cut(s) 262, 963
HinfI GANTC 5 cut(s) 5, 224, 699, 932, 1034
HpaII CCGG 3 cut(s) 221, 472, 980
HphI GGTGA 1 cut(s) 594
Hpy166II GTNNAC 2 cut(s) 262, 963
Hpy188I TCNGA 9 cut(s) 165, 203, 364, 517, 696, 770, 931, 937, 975
Hpy188III TCNNGA 2 cut(s) 290, 349
Hpy8I GTNNAC 2 cut(s) 262, 963
HpyAV CCTTC 6 cut(s) 47, 148, 170, 239, 371, 799
HpyCH4III ACNGT 3 cut(s) 75, 141, 532
HpyCH4IV ACGT 3 cut(s) 345, 467, 925
HpyCH4V TGCA 5 cut(s) 95, 130, 465, 508, 911
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 5 cut(s) 363, 695, 708, 715, 946
HpySE526I ACGT 3 cut(s) 345, 467, 925
Hsp92I GRCGYC 1 cut(s) 786
Hsp92II CATG 4 cut(s) 389, 567, 575, 988
HspAI GCGC 2 cut(s) 216, 218
Ksp22I TGATCA 1 cut(s) 811
Kzo9I GATC 6 cut(s) 45, 283, 512, 811, 866, 975
LmnI GCTCC 4 cut(s) 22, 61, 434, 1000
Lsp1109I GCAGC 1 cut(s) 873
LweI GCATC 4 cut(s) 156, 452, 746, 792
MaeI CTAG 3 cut(s) 65, 213, 431
MaeII ACGT 3 cut(s) 345, 467, 925
MaeIII GTNAC 2 cut(s) 30, 346
MalI GATC 6 cut(s) 47, 285, 514, 813, 868, 977
MboI GATC 6 cut(s) 45, 283, 512, 811, 866, 975
MboII GAAGA 4 cut(s) 401, 526, 533, 789
MfeI CAATTG 1 cut(s) 207
MlsI TGGCCA 2 cut(s) 329, 396
MluCI AATT 4 cut(s) 119, 207, 955, 967
MluNI TGGCCA 2 cut(s) 329, 396
MlyI GAGTC 3 cut(s) 233, 708, 1028
MnlI CCTC 2 cut(s) 72, 126
Mox20I TGGCCA 2 cut(s) 329, 396
MscI TGGCCA 2 cut(s) 329, 396
MslI CAYNNNNRTG 1 cut(s) 390
Msp20I TGGCCA 2 cut(s) 329, 396
MspA1I CMGCKG 1 cut(s) 402
MspI CCGG 3 cut(s) 221, 472, 980
MspR9I CCNGG 3 cut(s) 281, 980, 1041
MunI CAATTG 1 cut(s) 207
MvaI CCWGG 2 cut(s) 281, 1041
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 226
NciI CCSGG 1 cut(s) 980
NdeI CATATG 1 cut(s) 148
NdeII GATC 6 cut(s) 45, 283, 512, 811, 866, 975
NheI GCTAGC 2 cut(s) 64, 212
NlaIII CATG 4 cut(s) 389, 567, 575, 988
NlaIV GGNNCC 3 cut(s) 382, 436, 1002
NmuCI GTSAC 2 cut(s) 30, 346
NspI RCATGY 1 cut(s) 567
NspV TTCGAA 1 cut(s) 537
PauI GCGCGC 1 cut(s) 216
PciI ACATGT 1 cut(s) 563
PfeI GAWTC 2 cut(s) 5, 932
PfoI TCCNGGA 2 cut(s) 279, 978
PkrI GCNGC 3 cut(s) 578, 859, 888
PleI GAGTC 3 cut(s) 232, 707, 1028
PpsI GAGTC 3 cut(s) 232, 707, 1028
Ppu21I YACGTR 1 cut(s) 468
PpuMI RGGWCCY 3 cut(s) 248, 292, 380
PscI ACATGT 1 cut(s) 563
PshAI GACNNNNGTC 1 cut(s) 302
PsiI TTATAA 1 cut(s) 1054
Psp1406I AACGTT 1 cut(s) 925
Psp5II RGGWCCY 3 cut(s) 248, 292, 380
Psp6I CCWGG 2 cut(s) 279, 1039
PspFI CCCAGC 2 cut(s) 18, 890
PspGI CCWGG 2 cut(s) 279, 1039
PspN4I GGNNCC 3 cut(s) 382, 436, 1002
PspPI GGNCC 4 cut(s) 157, 248, 292, 380
PspPPI RGGWCCY 3 cut(s) 248, 292, 380
PstNI CAGNNNCTG 1 cut(s) 295
PteI GCGCGC 1 cut(s) 216
PvuII CAGCTG 1 cut(s) 402
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 1 cut(s) 390
SatI GCNGC 3 cut(s) 577, 858, 887
Sau3AI GATC 6 cut(s) 45, 283, 512, 811, 866, 975
Sau96I GGNCC 4 cut(s) 157, 248, 292, 380
SchI GAGTC 3 cut(s) 233, 708, 1028
ScrFI CCNGG 3 cut(s) 281, 980, 1041
SfaNI GCATC 4 cut(s) 156, 452, 746, 792
SfuI TTCGAA 1 cut(s) 537
SinI GGWCC 3 cut(s) 248, 292, 380
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
Sse9I AATT 4 cut(s) 119, 207, 955, 967
SsiI CCGC 6 cut(s) 180, 302, 576, 593, 858, 864
SspMI CTAG 3 cut(s) 65, 213, 431
StyD4I CCNGG 3 cut(s) 279, 978, 1039
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 3 cut(s) 75, 141, 532
TaiI ACGT 3 cut(s) 348, 470, 928
TaqI TCGA 3 cut(s) 537, 800, 1014
TasI AATT 4 cut(s) 119, 207, 955, 967
TauI GCSGC 2 cut(s) 579, 860
TfiI GAWTC 2 cut(s) 5, 932
TscAI CASTG 3 cut(s) 39, 1002, 1011
TseFI GTSAC 2 cut(s) 30, 346
TseI GCWGC 1 cut(s) 886
Tsp45I GTSAC 2 cut(s) 30, 346
TspDTI ATGAA 4 cut(s) 258, 402, 534, 747
TspRI CASTG 3 cut(s) 39, 1002, 1011
VpaK11BI GGWCC 3 cut(s) 248, 292, 380
XceI RCATGY 1 cut(s) 567
XmaJI CCTAGG 1 cut(s) 430
XspI CTAG 3 cut(s) 65, 213, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.