MD14G1123000.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003120
Physical Location & Seq
Reverse (-)
19736610 .. 19738943
2334 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1123000.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGATTCTAGTAAGAGCTGGGACCAGAAGTCACTGGTCAATGAGATCATTGAAGGACTGGAACTAGCAAAACAGTTGAGGCTAAGTCTGAACGCAAAATCGTCATCAGACAAGAAGCAATTTTTAGTGCAGAGGATACTATCGTCATACGAGAAGGCCCTTCTGATGCTGAAATGTAGCGGTTCGCCACAAAGTCCTATCGGGGCAATAACAAGCGTGCCGGAGTCGCTGATGTCAGCCAGCGCAGGTCCTTCTTGCGATGACAACAACAGAAGTCTCCAGGATCGTCATGACCTCACAGAGGTCTCCAAGAAAAGAAAGGAAATGGCCAAATGGACAGAACATGTCACGAGAGCTAGCTCTGAGAATGGGATGGAAGGAGCCCATGAAGATGGCCACTGCTGGAGAAAATATGGGCAGAAAGACATCCTAGGAACCAAACATCCGAGAAGCTATTACAGATGCACGTACCGGAACTCGGAAAGTTGTTGGGCTACAAAGCAGGTGCAAAGGTCGGATGAAGACCCCACTGTCTTCGAAATCACGTACAAAGGGAAGCATACATGTTCTCATGGCCGCAGTTCAGTTCCACCGCCACCATCACCAGAAAAGCAAGAGCGAAAACGACACAATCATAACGATACTTATCAGCAACAGCAGTCTCAAGGAAACCAAATCAGCTTCCCAACTAATCTGAGAGTCAATACTGAGAACTTGGACGACAGAGAGAACACGGCTTCTCCATTCTCTTTTACTTCAACTTCATTCGGATGTATGAACAACGATGACGCCTTTCTTTCATTAATGCTTGATGATAACAGTCTTTTGGACAATTTCAATCAATCATTGCTATCTCCGGCCGCGGGCGGATCAAACTATTACTTTGAGCCGGCAAGCCAGATGAGAAACATTGCAGGAAAAGAGCAACGTTCGGAATCTGATCTTGCAGAGATCGTCTCAGCCAAAAATTCGTCAACCAATTCTTCGATTCCGGACATTGATTTTTCACTGGAGCCAGTGGAACTCGACTCCTATTTCCCGTTTGACTGCCCAGGATTTTTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 WRKY

Protein Analysis

356

Amino Acids

39.87

Weight (kDa)

5.66

Isoelectric Point (pI)

65.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 130 - 189 2.3e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1066
AarI CACCTGC 1 cut(s) 493
AasI GACNNNNNNGTC 1 cut(s) 530
Acc36I ACCTGC 2 cut(s) 236, 493
AccII CGCG 1 cut(s) 863
AccIII TCCGGA 1 cut(s) 991
AciI CCGC 6 cut(s) 180, 577, 593, 861, 863, 867
AclI AACGTT 1 cut(s) 928
AclWI GGATC 2 cut(s) 291, 877
AcoI YGGCCR 4 cut(s) 327, 394, 574, 858
AcsI RAATTY 1 cut(s) 967
AcyI GRCGYC 1 cut(s) 789
AfaI GTAC 2 cut(s) 470, 548
AfiI CCNNNNNNNGG 3 cut(s) 301, 478, 863
AflIII ACRYGT 2 cut(s) 343, 563
AgsI TTSAA 3 cut(s) 53, 759, 838
AhdI GACNNNNNGTC 1 cut(s) 28
AjnI CCWGG 2 cut(s) 279, 1051
AloI GAACNNNNNNTCC 2 cut(s) 427, 459
AluBI AGCT 5 cut(s) 18, 356, 360, 453, 681
AluI AGCT 5 cut(s) 18, 356, 360, 453, 681
Alw26I GTCTC 4 cut(s) 281, 310, 666, 961
AlwI GGATC 2 cut(s) 291, 877
Aor13HI TCCGGA 1 cut(s) 991
AoxI GGCC 5 cut(s) 156, 327, 394, 574, 858
ApoI RAATTY 1 cut(s) 967
AseI ATTAAT 1 cut(s) 803
AspA2I CCTAGG 1 cut(s) 430
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 3 cut(s) 22, 157, 248
AsuHPI GGTGA 1 cut(s) 594
AsuII TTCGAA 1 cut(s) 537
AsuNHI GCTAGC 1 cut(s) 356
AvaII GGWCC 2 cut(s) 22, 248
AvrII CCTAGG 1 cut(s) 430
BaeI ACNNNNGTAYC 2 cut(s) 128, 161
BalI TGGCCA 2 cut(s) 329, 396
BanII GRGCYC 1 cut(s) 385
BauI CACGAG 1 cut(s) 349
BbsI GAAGAC 2 cut(s) 526, 528
BccI CCATC 3 cut(s) 367, 386, 607
BceAI ACGGC 1 cut(s) 750
BciT130I CCWGG 2 cut(s) 281, 1053
BciVI GTATCC 1 cut(s) 129
BcoDI GTCTC 4 cut(s) 281, 310, 666, 961
BfaI CTAG 4 cut(s) 9, 65, 357, 431
BfuAI ACCTGC 2 cut(s) 236, 493
BfuI GTATCC 1 cut(s) 129
BisI GCNGC 2 cut(s) 577, 861
BlnI CCTAGG 1 cut(s) 430
BlsI GCNGC 2 cut(s) 578, 862
Bme1390I CCNGG 2 cut(s) 281, 1053
Bme18I GGWCC 2 cut(s) 22, 248
BmeRI GACNNNNNGTC 1 cut(s) 28
BmgT120I GGNCC 3 cut(s) 22, 157, 248
BmiI GGNNCC 4 cut(s) 23, 382, 436, 1014
BmrFI CCNGG 2 cut(s) 281, 1053
BmsI GCATC 2 cut(s) 156, 452
BmtI GCTAGC 1 cut(s) 360
BpiI GAAGAC 2 cut(s) 526, 528
BplI GAGNNNNNCTC 2 cut(s) 941, 973
BpmI CTGGAG 3 cut(s) 263, 424, 1031
Bpu14I TTCGAA 1 cut(s) 537
BpuEI CTTGAG 1 cut(s) 648
BsaAI YACGTR 2 cut(s) 468, 546
BsaBI GATNNNNATC 1 cut(s) 645
BsaHI GRCGYC 1 cut(s) 789
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 3 cut(s) 430, 861, 1051
BsaWI WCCGGW 2 cut(s) 471, 991
BsaXI ACNNNNNCTCC 2 cut(s) 724, 754
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 478, 863
Bse118I RCCGGY 1 cut(s) 889
Bse1I ACTGG 4 cut(s) 39, 63, 1014, 1016
Bse3DI GCAATG 2 cut(s) 845, 909
Bse8I GATNNNNATC 1 cut(s) 645
BseAI TCCGGA 1 cut(s) 991
BseBI CCWGG 2 cut(s) 281, 1053
BseDI CCNNGG 3 cut(s) 430, 861, 1051
BseGI GGATG 5 cut(s) 378, 426, 442, 523, 776
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 3 cut(s) 301, 478, 863
BseMI GCAATG 2 cut(s) 845, 909
BseMII CTCAG 4 cut(s) 354, 686, 699, 972
BseNI ACTGG 4 cut(s) 39, 63, 1014, 1016
BseX3I CGGCCG 1 cut(s) 858
BseYI CCCAGC 1 cut(s) 18
BsgI GTGCAG 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 863
Bsh1285I CGRYCG 1 cut(s) 861
BshFI GGCC 5 cut(s) 158, 329, 396, 576, 860
BsiEI CGRYCG 1 cut(s) 861
BsiSI CCGG 5 cut(s) 221, 472, 857, 890, 992
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 3 cut(s) 301, 478, 863
BsmAI GTCTC 4 cut(s) 281, 310, 666, 961
BsmBI CGTCTC 1 cut(s) 961
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 5 cut(s) 158, 329, 396, 576, 860
Bso31I GGTCTC 1 cut(s) 310
Bsp119I TTCGAA 1 cut(s) 537
Bsp1286I GDGCHC 1 cut(s) 385
Bsp13I TCCGGA 1 cut(s) 991
Bsp143I GATC 5 cut(s) 45, 283, 869, 940, 951
BspACI CCGC 6 cut(s) 180, 577, 593, 861, 863, 867
BspANI GGCC 5 cut(s) 158, 329, 396, 576, 860
BspCNI CTCAG 4 cut(s) 355, 687, 700, 971
BspEI TCCGGA 1 cut(s) 991
BspFNI CGCG 1 cut(s) 863
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 4 cut(s) 23, 382, 436, 1014
BspMI ACCTGC 2 cut(s) 236, 493
BspOI GCTAGC 1 cut(s) 360
BspPI GGATC 2 cut(s) 291, 877
BspT104I TTCGAA 1 cut(s) 537
BspTNI GGTCTC 1 cut(s) 310
BsrDI GCAATG 2 cut(s) 845, 909
BsrFI RCCGGY 1 cut(s) 889
BsrI ACTGG 4 cut(s) 39, 63, 1014, 1016
BssAI RCCGGY 1 cut(s) 889
BssECI CCNNGG 3 cut(s) 430, 861, 1051
BssMI GATC 5 cut(s) 45, 283, 869, 940, 951
BssNI GRCGYC 1 cut(s) 789
BssSI CACGAG 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 430
Bst2BI CACGAG 1 cut(s) 349
Bst2UI CCWGG 2 cut(s) 281, 1053
Bst4CI ACNGT 3 cut(s) 75, 532, 821
BstACI GRCGYC 1 cut(s) 789
BstBAI YACGTR 2 cut(s) 468, 546
BstBI TTCGAA 1 cut(s) 537
BstC8I GCNNGC 6 cut(s) 218, 241, 358, 865, 891, 895
BstDEI CTNAG 5 cut(s) 83, 363, 695, 708, 958
BstDSI CCRYGG 1 cut(s) 861
BstENI CCTNNNNNAGG 1 cut(s) 299
BstF5I GGATG 5 cut(s) 378, 426, 442, 523, 776
BstFNI CGCG 1 cut(s) 863
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 5 cut(s) 48, 286, 872, 943, 954
BstMAI GTCTC 4 cut(s) 281, 310, 666, 961
BstMBI GATC 5 cut(s) 45, 283, 869, 940, 951
BstMCI CGRYCG 1 cut(s) 861
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 2 cut(s) 281, 1053
BstNSI RCATGY 2 cut(s) 347, 567
BstSCI CCNGG 2 cut(s) 279, 1051
BstUI CGCG 1 cut(s) 863
BstV2I GAAGAC 2 cut(s) 526, 528
BstXI CCANNNNNNTGG 1 cut(s) 392
BstZI CGGCCG 1 cut(s) 858
BsuI GTATCC 1 cut(s) 129
BsuRI GGCC 5 cut(s) 158, 329, 396, 576, 860
BtgI CCRYGG 1 cut(s) 861
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 5 cut(s) 378, 426, 442, 523, 776
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 5 cut(s) 32, 397, 528, 1007, 1023
BveI ACCTGC 2 cut(s) 236, 493
Cac8I GCNNGC 6 cut(s) 218, 241, 358, 865, 891, 895
CciI TCATGA 1 cut(s) 289
CfoI GCGC 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 889
Cfr13I GGNCC 3 cut(s) 22, 157, 248
Cfr42I CCGCGG 1 cut(s) 864
CseI GACGC 1 cut(s) 797
Csp6I GTAC 2 cut(s) 469, 547
CviAII CATG 5 cut(s) 290, 344, 386, 564, 572
CviQI GTAC 2 cut(s) 469, 547
DdeI CTNAG 5 cut(s) 83, 363, 695, 708, 958
DpnI GATC 5 cut(s) 47, 285, 871, 942, 953
DpnII GATC 5 cut(s) 45, 283, 869, 940, 951
DrdI GACNNNNNNGTC 1 cut(s) 530
DriI GACNNNNNGTC 1 cut(s) 28
DseDI GACNNNNNNGTC 1 cut(s) 530
EaeI YGGCCR 4 cut(s) 327, 394, 574, 858
EagI CGGCCG 1 cut(s) 858
Eam1105I GACNNNNNGTC 1 cut(s) 28
EciI GGCGGA 1 cut(s) 882
EclXI CGGCCG 1 cut(s) 858
Eco130I CCWWGG 1 cut(s) 430
Eco24I GRGCYC 1 cut(s) 385
Eco31I GGTCTC 1 cut(s) 310
Eco47I GGWCC 2 cut(s) 22, 248
Eco52I CGGCCG 1 cut(s) 858
EcoNI CCTNNNNNAGG 1 cut(s) 299
EcoO109I RGGNCCY 2 cut(s) 157, 248
EcoRII CCWGG 2 cut(s) 279, 1051
EcoT14I CCWWGG 1 cut(s) 430
EcoT38I GRGCYC 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 430
Esp3I CGTCTC 1 cut(s) 961
FaeI CATG 5 cut(s) 293, 347, 389, 567, 575
FaqI GGGAC 1 cut(s) 35
FatI CATG 5 cut(s) 289, 343, 385, 563, 571
FauI CCCGC 1 cut(s) 856
Fnu4HI GCNGC 2 cut(s) 577, 861
FokI GGATG 5 cut(s) 385, 413, 429, 530, 783
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 2 cut(s) 577, 861
FspBI CTAG 4 cut(s) 9, 65, 357, 431
GlaI GCGC 1 cut(s) 244
GluI GCNGC 2 cut(s) 577, 861
GsaI CCCAGC 1 cut(s) 22
GsuI CTGGAG 3 cut(s) 263, 424, 1031
HaeIII GGCC 5 cut(s) 158, 329, 396, 576, 860
HapII CCGG 5 cut(s) 221, 472, 857, 890, 992
HgaI GACGC 1 cut(s) 797
HhaI GCGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 789
Hin1II CATG 5 cut(s) 293, 347, 389, 567, 575
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HincII GTYRAC 1 cut(s) 975
HindII GTYRAC 1 cut(s) 975
HinfI GANTC 6 cut(s) 5, 224, 699, 935, 988, 1028
HpaII CCGG 5 cut(s) 221, 472, 857, 890, 992
HphI GGTGA 1 cut(s) 594
Hpy166II GTNNAC 1 cut(s) 975
Hpy188III TCNNGA 3 cut(s) 290, 349, 992
Hpy8I GTNNAC 1 cut(s) 975
HpyAV CCTTC 5 cut(s) 47, 148, 170, 261, 371
HpyCH4III ACNGT 3 cut(s) 75, 532, 821
HpyCH4IV ACGT 3 cut(s) 467, 545, 928
HpyCH4V TGCA 5 cut(s) 130, 465, 508, 914, 947
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 5 cut(s) 83, 363, 695, 708, 958
HpySE526I ACGT 3 cut(s) 467, 545, 928
Hsp92I GRCGYC 1 cut(s) 789
Hsp92II CATG 5 cut(s) 293, 347, 389, 567, 575
HspAI GCGC 1 cut(s) 243
Kpn2I TCCGGA 1 cut(s) 991
KroI GCCGGC 1 cut(s) 889
KroNI GCCGGC 1 cut(s) 891
KspI CCGCGG 1 cut(s) 864
Kzo9I GATC 5 cut(s) 45, 283, 869, 940, 951
LmnI GCTCC 2 cut(s) 380, 1012
LweI GCATC 2 cut(s) 156, 452
MaeI CTAG 4 cut(s) 9, 65, 357, 431
MaeII ACGT 3 cut(s) 467, 545, 928
MaeIII GTNAC 2 cut(s) 30, 346
MalI GATC 5 cut(s) 47, 285, 871, 942, 953
MboI GATC 5 cut(s) 45, 283, 869, 940, 951
MboII GAAGA 4 cut(s) 401, 526, 533, 975
MhlI GDGCHC 1 cut(s) 385
MlsI TGGCCA 2 cut(s) 329, 396
MluCI AATT 4 cut(s) 119, 832, 967, 979
MluNI TGGCCA 2 cut(s) 329, 396
MlyI GAGTC 3 cut(s) 233, 708, 1022
MmeI TCCRAC 1 cut(s) 495
MnlI CCTC 4 cut(s) 72, 126, 295, 305
Mox20I TGGCCA 2 cut(s) 329, 396
MroI TCCGGA 1 cut(s) 991
MroNI GCCGGC 1 cut(s) 889
MscI TGGCCA 2 cut(s) 329, 396
MseI TTAA 1 cut(s) 803
MslI CAYNNNNRTG 2 cut(s) 390, 769
Msp20I TGGCCA 2 cut(s) 329, 396
MspA1I CMGCKG 1 cut(s) 863
MspI CCGG 5 cut(s) 221, 472, 857, 890, 992
MspR9I CCNGG 2 cut(s) 281, 1053
MvaI CCWGG 2 cut(s) 281, 1053
MvnI CGCG 1 cut(s) 863
MwoI GCNNNNNNNGC 1 cut(s) 226
NaeI GCCGGC 1 cut(s) 891
NdeII GATC 5 cut(s) 45, 283, 869, 940, 951
NgoMIV GCCGGC 1 cut(s) 889
NheI GCTAGC 1 cut(s) 356
NlaIII CATG 5 cut(s) 293, 347, 389, 567, 575
NlaIV GGNNCC 4 cut(s) 23, 382, 436, 1014
NmuCI GTSAC 2 cut(s) 30, 346
NspI RCATGY 2 cut(s) 347, 567
NspV TTCGAA 1 cut(s) 537
PagI TCATGA 1 cut(s) 289
PaqCI CACCTGC 1 cut(s) 493
PciI ACATGT 2 cut(s) 343, 563
PdiI GCCGGC 1 cut(s) 891
PfeI GAWTC 3 cut(s) 5, 935, 988
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 2 cut(s) 578, 862
PleI GAGTC 3 cut(s) 232, 707, 1022
PpsI GAGTC 3 cut(s) 232, 707, 1022
Ppu21I YACGTR 2 cut(s) 468, 546
PpuMI RGGWCCY 1 cut(s) 248
PscI ACATGT 2 cut(s) 343, 563
PshBI ATTAAT 1 cut(s) 803
PsiI TTATAA 1 cut(s) 1066
Psp1406I AACGTT 1 cut(s) 928
Psp5II RGGWCCY 1 cut(s) 248
Psp6I CCWGG 2 cut(s) 279, 1051
PspFI CCCAGC 1 cut(s) 18
PspGI CCWGG 2 cut(s) 279, 1051
PspN4I GGNNCC 4 cut(s) 23, 382, 436, 1014
PspPI GGNCC 3 cut(s) 22, 157, 248
PspPPI RGGWCCY 1 cut(s) 248
RsaI GTAC 2 cut(s) 470, 548
RsaNI GTAC 2 cut(s) 469, 547
RseI CAYNNNNRTG 2 cut(s) 390, 769
SacII CCGCGG 1 cut(s) 864
SaqAI TTAA 1 cut(s) 803
SatI GCNGC 2 cut(s) 577, 861
Sau3AI GATC 5 cut(s) 45, 283, 869, 940, 951
Sau96I GGNCC 3 cut(s) 22, 157, 248
SchI GAGTC 3 cut(s) 233, 708, 1022
ScrFI CCNGG 2 cut(s) 281, 1053
SduI GDGCHC 1 cut(s) 385
SfaNI GCATC 2 cut(s) 156, 452
Sfr303I CCGCGG 1 cut(s) 864
SfuI TTCGAA 1 cut(s) 537
SgrBI CCGCGG 1 cut(s) 864
SinI GGWCC 2 cut(s) 22, 248
SmiMI CAYNNNNRTG 2 cut(s) 390, 769
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
Sse9I AATT 4 cut(s) 119, 832, 967, 979
SsiI CCGC 6 cut(s) 180, 577, 593, 861, 863, 867
SspMI CTAG 4 cut(s) 9, 65, 357, 431
StyD4I CCNGG 2 cut(s) 279, 1051
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 3 cut(s) 75, 532, 821
TaiI ACGT 3 cut(s) 470, 548, 931
TaqI TCGA 3 cut(s) 537, 986, 1026
TasI AATT 4 cut(s) 119, 832, 967, 979
TauI GCSGC 2 cut(s) 579, 863
TfiI GAWTC 3 cut(s) 5, 935, 988
Tru1I TTAA 1 cut(s) 803
Tru9I TTAA 1 cut(s) 803
TscAI CASTG 5 cut(s) 39, 404, 535, 1014, 1023
TseFI GTSAC 2 cut(s) 30, 346
Tsp45I GTSAC 2 cut(s) 30, 346
TspDTI ATGAA 5 cut(s) 402, 534, 753, 789, 791
TspRI CASTG 5 cut(s) 39, 404, 535, 1014, 1023
VpaK11BI GGWCC 2 cut(s) 22, 248
VspI ATTAAT 1 cut(s) 803
XagI CCTNNNNNAGG 1 cut(s) 299
XapI RAATTY 1 cut(s) 967
XceI RCATGY 2 cut(s) 347, 567
XmaJI CCTAGG 1 cut(s) 430
XspI CTAG 4 cut(s) 9, 65, 357, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.