Rh7BG150100
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0003120
Physical Location & Seq
Forward (+)
11133004 .. 11135035
2032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG150100.1

Sequence Viewer

Length: 759 bp
ATGTTTTGCAGAAAGATCATGGCCAAATGGACTGACCATGTCATAAGGGTTAGCTCTGAGAATGGGATTGAAGGACCTCATGAAGATGGCCATAGCTGGAGAAAATATGGGCAGAAAGATATTCTAGGAGCCAAATATCCTAGAAGCTATTATAGATGCACCTTCAGGAACACCCAAAACTGCTGGGCCACAAAGCAAGTGCAGAGATCAGATGAAGACCCTACCATATTTGAAATCACATACAAAGGAAAGCATACCTGCTCTCATGGCAGCACATCAGTTCCACCACCACCATCACCAGAACAGAAAGAACAAAAAAGACATAAGCGCGAGAACATTTCTGAACCACAGCAGTCTCAAGTGAACCAAATGAGCTTTCCAACTAATCTGAGGGTTGATACTGAGAATTTGGAAGACAGAGAGATGATGGCATCTCCATTCGCTTTTACTTCAACTTCATTTGGATGCATGATGAGTGGAGATGACTTCCTATCTCCGATGTTTGATAATGAGAATTTCTTTAGCGATTTCTCTCAACATTTGCTATCTCCAGCCGCAGGTGAATCAAATTACCACTTGGCACCACCAAGCCAAATGAGAAACATTGCAGTGAATGGGCAACTTTCAGAATCTGATCACACTGAGATAATCTCAGCCACCAATTCCTCAACCAATTCTCCAATTCCAGACATGGATTTCCCACTGGATCTAGTGGAATTCGACCCCAATTTCCAATTTGATGCTCCAGAATTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 WRKY

Protein Analysis

252

Amino Acids

28.81

Weight (kDa)

5.16

Isoelectric Point (pI)

61.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 28 - 87 6.1e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 548
Acc36I ACCTGC 2 cut(s) 266, 548
AccB1I GGYRCC 1 cut(s) 580
AccII CGCG 1 cut(s) 330
AciI CCGC 1 cut(s) 555
AclWI GGATC 1 cut(s) 714
AcoI YGGCCR 2 cut(s) 21, 88
AcsI RAATTY 4 cut(s) 406, 514, 716, 749
AcuI CTGAAG 1 cut(s) 148
AfiI CCNNNNNNNGG 1 cut(s) 557
AgsI TTSAA 3 cut(s) 71, 233, 453
AjuI GAANNNNNNNTTGG 2 cut(s) 360, 392
AluBI AGCT 4 cut(s) 54, 96, 147, 375
AluI AGCT 4 cut(s) 54, 96, 147, 375
Alw26I GTCTC 1 cut(s) 360
AlwI GGATC 1 cut(s) 714
AlwNI CAGNNNCTG 1 cut(s) 632
AoxI GGCC 3 cut(s) 21, 88, 186
ApeKI GCWGC 1 cut(s) 270
ApoI RAATTY 4 cut(s) 406, 514, 716, 749
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 2 cut(s) 74, 186
AsuHPI GGTGA 2 cut(s) 288, 572
AvaII GGWCC 1 cut(s) 74
BalI TGGCCA 2 cut(s) 23, 90
BanI GGYRCC 1 cut(s) 580
BbsI GAAGAC 2 cut(s) 222, 420
BbvI GCAGC 1 cut(s) 282
BccI CCATC 3 cut(s) 80, 301, 421
BclI TGATCA 1 cut(s) 634
BcoDI GTCTC 1 cut(s) 360
BfaI CTAG 3 cut(s) 125, 141, 710
BfuAI ACCTGC 2 cut(s) 266, 548
BisI GCNGC 2 cut(s) 271, 555
BlsI GCNGC 2 cut(s) 272, 556
Bme18I GGWCC 1 cut(s) 74
BmgT120I GGNCC 2 cut(s) 74, 186
BmiI GGNNCC 2 cut(s) 130, 582
BmsI GCATC 4 cut(s) 146, 440, 455, 730
BpiI GAAGAC 2 cut(s) 222, 420
BplI GAGNNNNNCTC 2 cut(s) 635, 667
BpmI CTGGAG 3 cut(s) 118, 534, 729
BpuEI CTTGAG 1 cut(s) 342
BsaXI ACNNNNNCTCC 2 cut(s) 661, 691
Bsc4I CCNNNNNNNGG 1 cut(s) 557
Bse1I ACTGG 1 cut(s) 708
Bse3DI GCAATG 1 cut(s) 603
BseGI GGATG 1 cut(s) 470
BseLI CCNNNNNNNGG 1 cut(s) 557
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 5 cut(s) 48, 380, 393, 633, 666
BseNI ACTGG 1 cut(s) 708
BseXI GCAGC 1 cut(s) 282
BseYI CCCAGC 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 221
Bsh1236I CGCG 1 cut(s) 330
BshFI GGCC 3 cut(s) 23, 90, 188
BshNI GGYRCC 1 cut(s) 580
BslI CCNNNNNNNGG 1 cut(s) 557
BsmAI GTCTC 1 cut(s) 360
BsnI GGCC 3 cut(s) 23, 90, 188
Bsp143I GATC 4 cut(s) 15, 206, 634, 706
BspACI CCGC 1 cut(s) 555
BspANI GGCC 3 cut(s) 23, 90, 188
BspCNI CTCAG 5 cut(s) 49, 381, 394, 634, 665
BspFNI CGCG 1 cut(s) 330
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 2 cut(s) 130, 582
BspMI ACCTGC 2 cut(s) 266, 548
BspPI GGATC 1 cut(s) 714
BspT107I GGYRCC 1 cut(s) 580
BsrDI GCAATG 1 cut(s) 603
BsrI ACTGG 1 cut(s) 708
BssMI GATC 4 cut(s) 15, 206, 634, 706
BstDEI CTNAG 5 cut(s) 57, 389, 402, 642, 652
BstF5I GGATG 1 cut(s) 470
BstFNI CGCG 1 cut(s) 330
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 4 cut(s) 18, 209, 637, 709
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 4 cut(s) 15, 206, 634, 706
BstMWI GCNNNNNNNGC 1 cut(s) 267
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 1 cut(s) 282
BstV2I GAAGAC 2 cut(s) 222, 420
BstX2I RGATCY 1 cut(s) 706
BstYI RGATCY 1 cut(s) 706
BsuRI GGCC 3 cut(s) 23, 90, 188
BtsCI GGATG 1 cut(s) 470
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 3 cut(s) 615, 639, 701
BveI ACCTGC 2 cut(s) 266, 548
CaiI CAGNNNCTG 1 cut(s) 632
CciI TCATGA 1 cut(s) 79
CfoI GCGC 1 cut(s) 330
Cfr13I GGNCC 2 cut(s) 74, 186
CviAII CATG 6 cut(s) 19, 38, 80, 266, 469, 691
DdeI CTNAG 5 cut(s) 57, 389, 402, 642, 652
DpnI GATC 4 cut(s) 17, 208, 636, 708
DpnII GATC 4 cut(s) 15, 206, 634, 706
EaeI YGGCCR 2 cut(s) 21, 88
Eco47I GGWCC 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 74
EcoRI GAATTC 1 cut(s) 716
EcoT22I ATGCAT 1 cut(s) 470
FaeI CATG 6 cut(s) 22, 41, 83, 269, 472, 694
FatI CATG 6 cut(s) 18, 37, 79, 265, 468, 690
FbaI TGATCA 1 cut(s) 634
Fnu4HI GCNGC 2 cut(s) 271, 555
FokI GGATG 1 cut(s) 477
Fsp4HI GCNGC 2 cut(s) 271, 555
FspBI CTAG 3 cut(s) 125, 141, 710
GlaI GCGC 1 cut(s) 329
GluI GCNGC 2 cut(s) 271, 555
GsaI CCCAGC 1 cut(s) 187
GsuI CTGGAG 3 cut(s) 118, 534, 729
HaeIII GGCC 3 cut(s) 23, 90, 188
HhaI GCGC 1 cut(s) 330
Hin1II CATG 6 cut(s) 22, 41, 83, 269, 472, 694
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HinfI GANTC 2 cut(s) 563, 629
HphI GGTGA 2 cut(s) 288, 572
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 8 cut(s) 58, 211, 343, 390, 498, 628, 634, 758
Hpy188III TCNNGA 4 cut(s) 80, 166, 686, 746
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 2 cut(s) 65, 172
HpyCH4V TGCA 5 cut(s) 9, 159, 202, 468, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 267
HpyF3I CTNAG 5 cut(s) 57, 389, 402, 642, 652
Hsp92II CATG 6 cut(s) 22, 41, 83, 269, 472, 694
HspAI GCGC 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 634
Kzo9I GATC 4 cut(s) 15, 206, 634, 706
LmnI GCTCC 2 cut(s) 128, 748
LpnPI CCDG 9 cut(s) 82, 151, 169, 271, 312, 543, 564, 689, 699
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 4 cut(s) 146, 440, 455, 730
MaeI CTAG 3 cut(s) 125, 141, 710
MalI GATC 4 cut(s) 17, 208, 636, 708
MboI GATC 4 cut(s) 15, 206, 634, 706
MboII GAAGA 3 cut(s) 95, 227, 425
MflI RGATCY 1 cut(s) 706
MlsI TGGCCA 2 cut(s) 23, 90
MluNI TGGCCA 2 cut(s) 23, 90
MmeI TCCRAC 1 cut(s) 404
MnlI CCTC 3 cut(s) 87, 384, 676
Mox20I TGGCCA 2 cut(s) 23, 90
Mph1103I ATGCAT 1 cut(s) 470
MscI TGGCCA 2 cut(s) 23, 90
MslI CAYNNNNRTG 3 cut(s) 84, 463, 608
Msp20I TGGCCA 2 cut(s) 23, 90
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 1 cut(s) 267
NdeII GATC 4 cut(s) 15, 206, 634, 706
NlaIII CATG 6 cut(s) 22, 41, 83, 269, 472, 694
NlaIV GGNNCC 2 cut(s) 130, 582
NsiI ATGCAT 1 cut(s) 470
PagI TCATGA 1 cut(s) 79
PaqCI CACCTGC 1 cut(s) 548
PfeI GAWTC 2 cut(s) 563, 629
PflFI GACNNNGTC 1 cut(s) 38
PkrI GCNGC 2 cut(s) 272, 556
PpuMI RGGWCCY 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 74
PspFI CCCAGC 1 cut(s) 183
PspN4I GGNNCC 2 cut(s) 130, 582
PspPI GGNCC 2 cut(s) 74, 186
PspPPI RGGWCCY 1 cut(s) 74
PstNI CAGNNNCTG 1 cut(s) 632
PsuI RGATCY 1 cut(s) 706
PsyI GACNNNGTC 1 cut(s) 38
RseI CAYNNNNRTG 3 cut(s) 84, 463, 608
SatI GCNGC 2 cut(s) 271, 555
Sau3AI GATC 4 cut(s) 15, 206, 634, 706
Sau96I GGNCC 2 cut(s) 74, 186
SetI ASST 8 cut(s) 56, 79, 98, 149, 164, 260, 377, 562
SfaNI GCATC 4 cut(s) 146, 440, 455, 730
SinI GGWCC 1 cut(s) 74
SmiMI CAYNNNNRTG 3 cut(s) 84, 463, 608
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
SsiI CCGC 1 cut(s) 555
SspMI CTAG 3 cut(s) 125, 141, 710
TaqI TCGA 1 cut(s) 720
TauI GCSGC 1 cut(s) 557
TfiI GAWTC 2 cut(s) 563, 629
TscAI CASTG 3 cut(s) 615, 646, 708
TseI GCWGC 1 cut(s) 270
TspDTI ATGAA 3 cut(s) 96, 228, 447
TspRI CASTG 3 cut(s) 615, 646, 708
Tth111I GACNNNGTC 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 74
XapI RAATTY 4 cut(s) 406, 514, 716, 749
XspI CTAG 3 cut(s) 125, 141, 710
Zsp2I ATGCAT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.