pycom06g09890
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0003120
Physical Location & Seq
Reverse (-)
15201774 .. 15203401
1628 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g09890.1

Sequence Viewer

Length: 1056 bp
ATGGATTCTGGTAAGAGCGGGGAGCAGAAGTCACTGGTCAATGAGATCATTGAAGGAATGGAGCTAGCAAAACAGTTGAGGCTATGTCTAAATGCAAAATCGTCAGCAGACACCAAGCAATTTTTAGTGCAGAGGATACTGTCGTCATATGAGAAGGCCCTTCTGATGCTGAAATGTGGTGGTTCGCCACAAAGTCCTATCAGAGCAATTGCTAGCGCACCGGAGTCGCTAATGTCAGCCAATGAAGGACCTTGTTGCGTTGACAATAACAGCAGTCTCCAGGATCATCAGGACCTGACGGCGGTCTCCAAGAAAAGAAAGGAAATGGCCAAATGGACAGAACATGTCACGAGAGTAATCTCTGAGAATGGGATTGAAGGACCCCATGAAGATGGCCACAGCTGGAGAAAATATGGGCAAAAAGACATCCTAGGAGCCAAACATCCAAGAAGCTATTACAGATGCACGTACCGGAACACGCAGAATTGTTGGGCTACAAAGCAAGTGCAAAGATCAGACGAAGACCCCACCGTCTTCGAAATCACATACAAAGGGAAGCATACATGTTCTCATGGCGGCAGTTCAGTTCCACCGCCACCATCACCAGAAAAGCAAGAACGAAAACGACACAATCATAACAATACTTATCAACAACAGCAGTCTCAAGGAAACCAAATGAGCTTCCCAACTAATCTGAGAGTCAATACTGAGAACTTAGACGACAGAGAGAACACAGCGTCTCCATTCTCTTTTACTTCATCTCCATTCGGAAGTATCAGCGATGACGCCTTCCTATCTTCGATGCTTGATGATAAAAGTCTTTTTGACAATTTCAATCAATCATTCCTGTCTCCAGCCGCAGGCGGATCAAACTATTACTTTGCGCTGCCTAGCCAGATGAGAAACATTGCAGGATATGAGCAACGTTCGGAATCTGATATCATCTCAGCCAACAATTCGTCAACCAATTCTCCGATTCCGGACATGGATTTTCCACTGCAGCCAGTCGAACTCGACCCCTATTTCCCATTTGACTCCCCAGGATTTTTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 WRKY

Protein Analysis

352

Amino Acids

39.25

Weight (kDa)

5.88

Isoelectric Point (pI)

64.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 130 - 189 1.7e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1054
AasI GACNNNNNNGTC 1 cut(s) 530
AccBSI CCGCTC 1 cut(s) 18
AccIII TCCGGA 1 cut(s) 979
AciI CCGC 6 cut(s) 18, 302, 576, 593, 858, 864
AclI AACGTT 1 cut(s) 925
AclWI GGATC 2 cut(s) 291, 874
AcoI YGGCCR 2 cut(s) 327, 394
AcyI GRCGYC 1 cut(s) 786
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 2 cut(s) 301, 860
AflIII ACRYGT 2 cut(s) 343, 563
AgsI TTSAA 3 cut(s) 53, 377, 835
AjnI CCWGG 2 cut(s) 279, 1039
AluBI AGCT 4 cut(s) 64, 402, 453, 681
AluI AGCT 4 cut(s) 64, 402, 453, 681
Alw26I GTCTC 5 cut(s) 281, 310, 666, 744, 855
AlwI GGATC 2 cut(s) 291, 874
AlwNI CAGNNNCTG 1 cut(s) 295
Aor13HI TCCGGA 1 cut(s) 979
AoxI GGCC 3 cut(s) 156, 327, 394
ApeKI GCWGC 2 cut(s) 886, 1000
AspA2I CCTAGG 1 cut(s) 430
AspLEI GCGC 2 cut(s) 218, 886
AspS9I GGNCC 4 cut(s) 157, 248, 292, 380
AsuHPI GGTGA 1 cut(s) 594
AsuII TTCGAA 1 cut(s) 537
AsuNHI GCTAGC 2 cut(s) 64, 212
AvaII GGWCC 3 cut(s) 248, 292, 380
AvrII CCTAGG 1 cut(s) 430
BaeI ACNNNNGTAYC 2 cut(s) 128, 161
BalI TGGCCA 2 cut(s) 329, 396
BauI CACGAG 1 cut(s) 349
BbsI GAAGAC 2 cut(s) 526, 528
BbvI GCAGC 2 cut(s) 873, 1012
BccI CCATC 2 cut(s) 386, 607
BceAI ACGGC 1 cut(s) 315
BcgI CGANNNNNNTGC 2 cut(s) 207, 241
BciT130I CCWGG 2 cut(s) 281, 1041
BciVI GTATCC 1 cut(s) 129
BcoDI GTCTC 5 cut(s) 281, 310, 666, 744, 855
BfaI CTAG 4 cut(s) 65, 213, 431, 891
BfmI CTRYAG 1 cut(s) 998
BfuI GTATCC 1 cut(s) 129
BisI GCNGC 4 cut(s) 577, 858, 887, 1001
BlnI CCTAGG 1 cut(s) 430
BlsI GCNGC 4 cut(s) 578, 859, 888, 1002
Bme1390I CCNGG 2 cut(s) 281, 1041
Bme18I GGWCC 3 cut(s) 248, 292, 380
BmgT120I GGNCC 4 cut(s) 157, 248, 292, 380
BmiI GGNNCC 2 cut(s) 382, 436
BmrFI CCNGG 2 cut(s) 281, 1041
BmsI GCATC 3 cut(s) 156, 452, 792
BmtI GCTAGC 2 cut(s) 68, 216
BoxI GACNNNNGTC 1 cut(s) 302
BpiI GAAGAC 2 cut(s) 526, 528
BpmI CTGGAG 3 cut(s) 263, 424, 837
Bpu14I TTCGAA 1 cut(s) 537
BpuEI CTTGAG 1 cut(s) 648
BsaAI YACGTR 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 786
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 430, 1039
BsaWI WCCGGW 3 cut(s) 220, 471, 979
BsaXI ACNNNNNCTCC 8 cut(s) 14, 44, 724, 745, 754, 775, 955, 985
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 860
Bse1I ACTGG 2 cut(s) 39, 1004
Bse3DI GCAATG 1 cut(s) 906
BseAI TCCGGA 1 cut(s) 979
BseBI CCWGG 2 cut(s) 281, 1041
BseDI CCNNGG 2 cut(s) 430, 1039
BseGI GGATG 2 cut(s) 426, 442
BseLI CCNNNNNNNGG 2 cut(s) 301, 860
BseMI GCAATG 1 cut(s) 906
BseMII CTCAG 4 cut(s) 354, 686, 699, 960
BseNI ACTGG 2 cut(s) 39, 1004
BseXI GCAGC 2 cut(s) 873, 1012
BsgI GTGCAG 1 cut(s) 149
BshFI GGCC 3 cut(s) 158, 329, 396
BsiSI CCGG 3 cut(s) 221, 472, 980
BslI CCNNNNNNNGG 2 cut(s) 301, 860
BsmAI GTCTC 5 cut(s) 281, 310, 666, 744, 855
BsmBI CGTCTC 1 cut(s) 744
BsnI GGCC 3 cut(s) 158, 329, 396
Bso31I GGTCTC 1 cut(s) 310
Bsp119I TTCGAA 1 cut(s) 537
Bsp13I TCCGGA 1 cut(s) 979
Bsp143I GATC 4 cut(s) 45, 283, 512, 866
BspACI CCGC 6 cut(s) 18, 302, 576, 593, 858, 864
BspANI GGCC 3 cut(s) 158, 329, 396
BspCNI CTCAG 4 cut(s) 355, 687, 700, 959
BspEI TCCGGA 1 cut(s) 979
BspLI GGNNCC 2 cut(s) 382, 436
BspMAI CTGCAG 1 cut(s) 1002
BspOI GCTAGC 2 cut(s) 68, 216
BspPI GGATC 2 cut(s) 291, 874
BspT104I TTCGAA 1 cut(s) 537
BspTNI GGTCTC 1 cut(s) 310
BsrBI CCGCTC 1 cut(s) 18
BsrDI GCAATG 1 cut(s) 906
BsrI ACTGG 2 cut(s) 39, 1004
BssECI CCNNGG 2 cut(s) 430, 1039
BssMI GATC 4 cut(s) 45, 283, 512, 866
BssNI GRCGYC 1 cut(s) 786
BssSI CACGAG 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 430
Bst2BI CACGAG 1 cut(s) 349
Bst2UI CCWGG 2 cut(s) 281, 1041
Bst4CI ACNGT 3 cut(s) 75, 141, 532
BstACI GRCGYC 1 cut(s) 786
BstBAI YACGTR 1 cut(s) 468
BstBI TTCGAA 1 cut(s) 537
BstC8I GCNNGC 3 cut(s) 66, 214, 862
BstDEI CTNAG 5 cut(s) 363, 695, 708, 715, 946
BstF5I GGATG 2 cut(s) 426, 442
BstHHI GCGC 2 cut(s) 218, 886
BstKTI GATC 4 cut(s) 48, 286, 515, 869
BstMAI GTCTC 5 cut(s) 281, 310, 666, 744, 855
BstMBI GATC 4 cut(s) 45, 283, 512, 866
BstNI CCWGG 2 cut(s) 281, 1041
BstNSI RCATGY 2 cut(s) 347, 567
BstPAI GACNNNNGTC 1 cut(s) 302
BstSCI CCNGG 2 cut(s) 279, 1039
BstSFI CTRYAG 1 cut(s) 998
BstV1I GCAGC 2 cut(s) 873, 1012
BstV2I GAAGAC 2 cut(s) 526, 528
BstXI CCANNNNNNTGG 1 cut(s) 392
BsuI GTATCC 1 cut(s) 129
BsuRI GGCC 3 cut(s) 158, 329, 396
BtgZI GCGATG 1 cut(s) 795
BtsCI GGATG 2 cut(s) 426, 442
BtsI GCAGTG 1 cut(s) 995
BtsIMutI CAGTG 2 cut(s) 32, 995
Cac8I GCNNGC 3 cut(s) 66, 214, 862
CaiI CAGNNNCTG 1 cut(s) 295
CfoI GCGC 2 cut(s) 218, 886
Cfr13I GGNCC 4 cut(s) 157, 248, 292, 380
CseI GACGC 2 cut(s) 726, 794
Csp6I GTAC 1 cut(s) 469
CviAII CATG 5 cut(s) 344, 386, 564, 572, 985
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 5 cut(s) 363, 695, 708, 715, 946
DpnI GATC 4 cut(s) 47, 285, 514, 868
DpnII GATC 4 cut(s) 45, 283, 512, 866
DrdI GACNNNNNNGTC 1 cut(s) 530
DseDI GACNNNNNNGTC 1 cut(s) 530
EaeI YGGCCR 2 cut(s) 327, 394
EciI GGCGGA 1 cut(s) 879
Eco130I CCWWGG 1 cut(s) 430
Eco31I GGTCTC 1 cut(s) 310
Eco32I GATATC 1 cut(s) 940
Eco47I GGWCC 3 cut(s) 248, 292, 380
EcoO109I RGGNCCY 4 cut(s) 157, 248, 292, 380
EcoRII CCWGG 2 cut(s) 279, 1039
EcoRV GATATC 1 cut(s) 940
EcoT14I CCWWGG 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 430
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 5 cut(s) 347, 389, 567, 575, 988
FatI CATG 5 cut(s) 343, 385, 563, 571, 984
FauI CCCGC 1 cut(s) 11
FauNDI CATATG 1 cut(s) 148
Fnu4HI GCNGC 4 cut(s) 577, 858, 887, 1001
FokI GGATG 2 cut(s) 413, 429
Fsp4HI GCNGC 4 cut(s) 577, 858, 887, 1001
FspBI CTAG 4 cut(s) 65, 213, 431, 891
GlaI GCGC 2 cut(s) 217, 885
GluI GCNGC 4 cut(s) 577, 858, 887, 1001
GsuI CTGGAG 3 cut(s) 263, 424, 837
HaeIII GGCC 3 cut(s) 158, 329, 396
HapII CCGG 3 cut(s) 221, 472, 980
HgaI GACGC 2 cut(s) 726, 794
HhaI GCGC 2 cut(s) 218, 886
Hin1I GRCGYC 1 cut(s) 786
Hin1II CATG 5 cut(s) 347, 389, 567, 575, 988
Hin6I GCGC 2 cut(s) 216, 884
HinP1I GCGC 2 cut(s) 216, 884
HincII GTYRAC 2 cut(s) 262, 963
HindII GTYRAC 2 cut(s) 262, 963
HinfI GANTC 6 cut(s) 5, 224, 699, 932, 976, 1034
HpaII CCGG 3 cut(s) 221, 472, 980
HphI GGTGA 1 cut(s) 594
Hpy166II GTNNAC 2 cut(s) 262, 963
Hpy188I TCNGA 9 cut(s) 165, 203, 364, 517, 696, 770, 931, 937, 975
Hpy188III TCNNGA 3 cut(s) 290, 349, 980
Hpy8I GTNNAC 2 cut(s) 262, 963
HpyAV CCTTC 6 cut(s) 47, 148, 170, 239, 371, 799
HpyCH4III ACNGT 3 cut(s) 75, 141, 532
HpyCH4IV ACGT 2 cut(s) 467, 925
HpyCH4V TGCA 6 cut(s) 95, 130, 465, 508, 911, 1000
HpyF3I CTNAG 5 cut(s) 363, 695, 708, 715, 946
HpySE526I ACGT 2 cut(s) 467, 925
Hsp92I GRCGYC 1 cut(s) 786
Hsp92II CATG 5 cut(s) 347, 389, 567, 575, 988
HspAI GCGC 2 cut(s) 216, 884
Kpn2I TCCGGA 1 cut(s) 979
Kzo9I GATC 4 cut(s) 45, 283, 512, 866
LmnI GCTCC 3 cut(s) 22, 61, 434
Lsp1109I GCAGC 2 cut(s) 873, 1012
LweI GCATC 3 cut(s) 156, 452, 792
MaeI CTAG 4 cut(s) 65, 213, 431, 891
MaeII ACGT 2 cut(s) 467, 925
MaeIII GTNAC 2 cut(s) 30, 346
MalI GATC 4 cut(s) 47, 285, 514, 868
MbiI CCGCTC 1 cut(s) 18
MboI GATC 4 cut(s) 45, 283, 512, 866
MboII GAAGA 4 cut(s) 401, 526, 533, 789
MfeI CAATTG 1 cut(s) 207
MlsI TGGCCA 2 cut(s) 329, 396
MluCI AATT 6 cut(s) 119, 207, 484, 829, 955, 967
MluNI TGGCCA 2 cut(s) 329, 396
MlyI GAGTC 3 cut(s) 233, 708, 1028
MnlI CCTC 2 cut(s) 72, 126
Mox20I TGGCCA 2 cut(s) 329, 396
MroI TCCGGA 1 cut(s) 979
MscI TGGCCA 2 cut(s) 329, 396
MslI CAYNNNNRTG 1 cut(s) 390
Msp20I TGGCCA 2 cut(s) 329, 396
MspA1I CMGCKG 1 cut(s) 402
MspI CCGG 3 cut(s) 221, 472, 980
MspR9I CCNGG 2 cut(s) 281, 1041
MunI CAATTG 1 cut(s) 207
MvaI CCWGG 2 cut(s) 281, 1041
NdeI CATATG 1 cut(s) 148
NdeII GATC 4 cut(s) 45, 283, 512, 866
NheI GCTAGC 2 cut(s) 64, 212
NlaIII CATG 5 cut(s) 347, 389, 567, 575, 988
NlaIV GGNNCC 2 cut(s) 382, 436
NmuCI GTSAC 2 cut(s) 30, 346
NspI RCATGY 2 cut(s) 347, 567
NspV TTCGAA 1 cut(s) 537
PciI ACATGT 2 cut(s) 343, 563
PfeI GAWTC 3 cut(s) 5, 932, 976
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 4 cut(s) 578, 859, 888, 1002
PleI GAGTC 3 cut(s) 232, 707, 1028
PpsI GAGTC 3 cut(s) 232, 707, 1028
Ppu21I YACGTR 1 cut(s) 468
PpuMI RGGWCCY 3 cut(s) 248, 292, 380
PscI ACATGT 2 cut(s) 343, 563
PshAI GACNNNNGTC 1 cut(s) 302
PsiI TTATAA 1 cut(s) 1054
Psp1406I AACGTT 1 cut(s) 925
Psp5II RGGWCCY 3 cut(s) 248, 292, 380
Psp6I CCWGG 2 cut(s) 279, 1039
PspGI CCWGG 2 cut(s) 279, 1039
PspN4I GGNNCC 2 cut(s) 382, 436
PspPI GGNCC 4 cut(s) 157, 248, 292, 380
PspPPI RGGWCCY 3 cut(s) 248, 292, 380
PstI CTGCAG 1 cut(s) 1002
PstNI CAGNNNCTG 1 cut(s) 295
PvuII CAGCTG 1 cut(s) 402
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 1 cut(s) 390
SatI GCNGC 4 cut(s) 577, 858, 887, 1001
Sau3AI GATC 4 cut(s) 45, 283, 512, 866
Sau96I GGNCC 4 cut(s) 157, 248, 292, 380
SchI GAGTC 3 cut(s) 233, 708, 1028
ScrFI CCNGG 2 cut(s) 281, 1041
SetI ASST 8 cut(s) 66, 253, 297, 404, 455, 470, 683, 928
SfaNI GCATC 3 cut(s) 156, 452, 792
SfcI CTRYAG 1 cut(s) 998
SfuI TTCGAA 1 cut(s) 537
SinI GGWCC 3 cut(s) 248, 292, 380
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
Sse9I AATT 6 cut(s) 119, 207, 484, 829, 955, 967
SsiI CCGC 6 cut(s) 18, 302, 576, 593, 858, 864
SspMI CTAG 4 cut(s) 65, 213, 431, 891
StyD4I CCNGG 2 cut(s) 279, 1039
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 3 cut(s) 75, 141, 532
TaiI ACGT 2 cut(s) 470, 928
TaqI TCGA 4 cut(s) 537, 800, 1008, 1014
TasI AATT 6 cut(s) 119, 207, 484, 829, 955, 967
TauI GCSGC 2 cut(s) 579, 860
TfiI GAWTC 3 cut(s) 5, 932, 976
TscAI CASTG 2 cut(s) 39, 1002
TseFI GTSAC 2 cut(s) 30, 346
TseI GCWGC 2 cut(s) 886, 1000
Tsp45I GTSAC 2 cut(s) 30, 346
TspDTI ATGAA 3 cut(s) 258, 402, 747
TspRI CASTG 2 cut(s) 39, 1002
VpaK11BI GGWCC 3 cut(s) 248, 292, 380
XceI RCATGY 2 cut(s) 347, 567
XmaJI CCTAGG 1 cut(s) 430
XspI CTAG 4 cut(s) 65, 213, 431, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.