RLG00000004139
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003120
Physical Location & Seq
Reverse (-)
56525363 .. 56526884
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004139

Sequence Viewer

Length: 1092 bp
ATGGATACTGCTAGTAAGAGCTGGGAGCAGAAGTCATTAGTTGGGGAGATCATTCAAGGGATGGAGCTAGCACAACAGTTGAGGCAAAGTCTGAGTGCAACATCAACTTCATCAGAAACTCGGGAGTTTTTAGTGCAAAGGATATTGACCTCGTACGAGAAGGCCCTTTTGCTACTGCAATTGAGTGGCACGCCTCAAACATCTCATGTGGATGCAAAATCCATTGTGCCTCAGGAGTCTCGAATGTCTGCCAACACAAGTCCTTGTGGTGATGACTATAACCACAGGAGTTTCAAGGATCATCAGGACCTGCACGAAATCTCCAAAAAGAGAAAGATCATGGCCAAATGGACTGACCATGTCATAAGGGTTAGCTCTGAGAATGGGATTGAAGGACCTCATGAAGATGGCCATAGCTGGAGAAAATATGGGCAGAAAGATATTCTAGGAGCCAAATATCCTAGAAGCTACTATAGATGCACCTTCAGGAACACACAAAACTGCTGGGCTACAAAGCAAGTGCAGAGATCAGATGAAGACCCTACCATATTTGAAATCACATACAAAGGAAAGCATACCTGCTCTCATGGCAGCACATCAGTTCCACCACCACCATCACCAGAACAGCAAGAACAAAGAAGACATAAGCGCGAGAACATTTCTGAACCACAGCAGTCTCAACAGCAGTCTCAAGTGAACCAAATGAGCTTTCCAACTAATCTGAGGGTTGATACTGAGAATTTGGAAGACAGAGAGATGATAGCATCTCCGTTCGCTTTTACTTCAACTTCATTTGGATGCATGATGAGTGGAGATGACTTCCTATCTCCAATGTTTGATAATGAGAATTTCTTTAGCGATTTCTCTCAACATTTGCTATCTCCAGCCGCTGGCGAATCAAATTACCACTTGGCACCACCAAGCCAAATGAGAAACATTGCAGTGAACGGGCAACTTACAGAATCTGATCACACTGAGATAATCTCAGCCACCAATTCCTCAACCAATTCTCCAATTCCAGACATGGATTTCCCACTGGATCTAGTGGAATTCGACCCCAATTTCCAATTTGATGCTCCAGAACTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 WRKY

Protein Analysis

364

Amino Acids

41.21

Weight (kDa)

5.31

Isoelectric Point (pI)

62.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 135 - 194 1.1e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 318, 587
AccB1I GGYRCC 1 cut(s) 913
AccB7I CCANNNNNTGG 1 cut(s) 890
AccII CGCG 1 cut(s) 651
AciI CCGC 1 cut(s) 888
AclWI GGATC 2 cut(s) 306, 1047
AcoI YGGCCR 2 cut(s) 342, 409
AcsI RAATTY 3 cut(s) 739, 847, 1049
AcuI CTGAAG 1 cut(s) 469
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 890
AgsI TTSAA 5 cut(s) 56, 295, 392, 554, 786
AjuI GAANNNNNNNTTGG 2 cut(s) 693, 725
AluBI AGCT 6 cut(s) 21, 67, 375, 417, 468, 708
AluI AGCT 6 cut(s) 21, 67, 375, 417, 468, 708
Alw26I GTCTC 3 cut(s) 243, 681, 693
AlwI GGATC 2 cut(s) 306, 1047
AlwNI CAGNNNCTG 3 cut(s) 310, 890, 965
Ama87I CYCGRG 1 cut(s) 120
AoxI GGCC 3 cut(s) 162, 342, 409
ApeKI GCWGC 1 cut(s) 591
ApoI RAATTY 3 cut(s) 739, 847, 1049
AspLEI GCGC 1 cut(s) 651
AspS9I GGNCC 3 cut(s) 163, 307, 395
AsuHPI GGTGA 2 cut(s) 281, 609
AsuNHI GCTAGC 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 2 cut(s) 307, 395
AxyI CCTNAGG 1 cut(s) 231
BalI TGGCCA 2 cut(s) 344, 411
BanI GGYRCC 1 cut(s) 913
BbsI GAAGAC 3 cut(s) 543, 646, 753
BbvI GCAGC 1 cut(s) 603
BccI CCATC 3 cut(s) 55, 401, 622
BclI TGATCA 1 cut(s) 967
BcoDI GTCTC 3 cut(s) 243, 681, 693
BfaI CTAG 5 cut(s) 12, 68, 446, 462, 1043
BfmI CTRYAG 1 cut(s) 472
BfuAI ACCTGC 2 cut(s) 318, 587
BisI GCNGC 2 cut(s) 592, 888
BlsI GCNGC 2 cut(s) 593, 889
Bme18I GGWCC 2 cut(s) 307, 395
BmeT110I CYCGRG 1 cut(s) 120
BmgT120I GGNCC 3 cut(s) 163, 307, 395
BmiI GGNNCC 2 cut(s) 451, 915
BmsI GCATC 5 cut(s) 202, 467, 773, 788, 1063
BmtI GCTAGC 1 cut(s) 71
BpiI GAAGAC 3 cut(s) 543, 646, 753
BplI GAGNNNNNCTC 2 cut(s) 968, 1000
BpmI CTGGAG 3 cut(s) 439, 867, 1062
BpuEI CTTGAG 1 cut(s) 675
BsaXI ACNNNNNCTCC 6 cut(s) 17, 47, 305, 335, 994, 1024
Bsc4I CCNNNNNNNGG 1 cut(s) 890
Bse1I ACTGG 1 cut(s) 1041
Bse21I CCTNAGG 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 936
BseGI GGATG 3 cut(s) 66, 217, 803
BseLI CCNNNNNNNGG 1 cut(s) 890
BseMI GCAATG 1 cut(s) 936
BseMII CTCAG 7 cut(s) 83, 245, 369, 713, 726, 966, 999
BseNI ACTGG 1 cut(s) 1041
BseXI GCAGC 1 cut(s) 603
BseYI CCCAGC 2 cut(s) 21, 504
BsgI GTGCAG 2 cut(s) 296, 542
Bsh1236I CGCG 1 cut(s) 651
BshFI GGCC 3 cut(s) 164, 344, 411
BshNI GGYRCC 1 cut(s) 913
BsiHKCI CYCGRG 1 cut(s) 120
BsiWI CGTACG 1 cut(s) 153
BslI CCNNNNNNNGG 1 cut(s) 890
BsmAI GTCTC 3 cut(s) 243, 681, 693
BsnI GGCC 3 cut(s) 164, 344, 411
BsoBI CYCGRG 1 cut(s) 120
Bsp143I GATC 6 cut(s) 48, 298, 336, 527, 967, 1039
BspACI CCGC 1 cut(s) 888
BspANI GGCC 3 cut(s) 164, 344, 411
BspCNI CTCAG 7 cut(s) 84, 244, 370, 714, 727, 967, 998
BspFNI CGCG 1 cut(s) 651
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 451, 915
BspMI ACCTGC 2 cut(s) 318, 587
BspOI GCTAGC 1 cut(s) 71
BspPI GGATC 2 cut(s) 306, 1047
BspT107I GGYRCC 1 cut(s) 913
BsrDI GCAATG 1 cut(s) 936
BsrI ACTGG 1 cut(s) 1041
BssMI GATC 6 cut(s) 48, 298, 336, 527, 967, 1039
Bst4CI ACNGT 1 cut(s) 78
BstC8I GCNNGC 3 cut(s) 69, 191, 892
BstDEI CTNAG 7 cut(s) 92, 231, 378, 722, 735, 975, 985
BstF5I GGATG 3 cut(s) 66, 217, 803
BstFNI CGCG 1 cut(s) 651
BstHHI GCGC 1 cut(s) 651
BstKTI GATC 6 cut(s) 51, 301, 339, 530, 970, 1042
BstMAI GTCTC 3 cut(s) 243, 681, 693
BstMBI GATC 6 cut(s) 48, 298, 336, 527, 967, 1039
BstMWI GCNNNNNNNGC 1 cut(s) 588
BstSFI CTRYAG 1 cut(s) 472
BstUI CGCG 1 cut(s) 651
BstV1I GCAGC 1 cut(s) 603
BstV2I GAAGAC 3 cut(s) 543, 646, 753
BstX2I RGATCY 1 cut(s) 1039
BstYI RGATCY 1 cut(s) 1039
Bsu36I CCTNAGG 1 cut(s) 231
BsuRI GGCC 3 cut(s) 164, 344, 411
BtsCI GGATG 3 cut(s) 66, 217, 803
BtsI GCAGTG 1 cut(s) 948
BtsIMutI CAGTG 3 cut(s) 948, 972, 1034
BveI ACCTGC 2 cut(s) 318, 587
Cac8I GCNNGC 3 cut(s) 69, 191, 892
CaiI CAGNNNCTG 3 cut(s) 310, 890, 965
CciI TCATGA 1 cut(s) 400
CfoI GCGC 1 cut(s) 651
Cfr13I GGNCC 3 cut(s) 163, 307, 395
Csp6I GTAC 1 cut(s) 154
CviAII CATG 7 cut(s) 206, 340, 359, 401, 587, 802, 1024
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 7 cut(s) 92, 231, 378, 722, 735, 975, 985
DpnI GATC 6 cut(s) 50, 300, 338, 529, 969, 1041
DpnII GATC 6 cut(s) 48, 298, 336, 527, 967, 1039
EaeI YGGCCR 2 cut(s) 342, 409
Eco47I GGWCC 2 cut(s) 307, 395
Eco57I CTGAAG 1 cut(s) 469
Eco81I CCTNAGG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 120
EcoO109I RGGNCCY 3 cut(s) 163, 307, 395
EcoRI GAATTC 1 cut(s) 1049
EcoT22I ATGCAT 1 cut(s) 803
FaeI CATG 7 cut(s) 209, 343, 362, 404, 590, 805, 1027
FatI CATG 7 cut(s) 205, 339, 358, 400, 586, 801, 1023
FbaI TGATCA 1 cut(s) 967
Fnu4HI GCNGC 2 cut(s) 592, 888
FokI GGATG 3 cut(s) 73, 224, 810
Fsp4HI GCNGC 2 cut(s) 592, 888
FspBI CTAG 5 cut(s) 12, 68, 446, 462, 1043
GlaI GCGC 1 cut(s) 650
GluI GCNGC 2 cut(s) 592, 888
GsaI CCCAGC 2 cut(s) 25, 508
GsuI CTGGAG 3 cut(s) 439, 867, 1062
HaeIII GGCC 3 cut(s) 164, 344, 411
HhaI GCGC 1 cut(s) 651
Hin1II CATG 7 cut(s) 209, 343, 362, 404, 590, 805, 1027
Hin6I GCGC 1 cut(s) 649
HinP1I GCGC 1 cut(s) 649
HinfI GANTC 3 cut(s) 236, 896, 962
HphI GGTGA 2 cut(s) 281, 609
Hpy166II GTNNAC 2 cut(s) 697, 946
Hpy188I TCNGA 8 cut(s) 93, 115, 379, 532, 664, 723, 967, 1091
Hpy188III TCNNGA 8 cut(s) 122, 233, 240, 305, 401, 487, 1019, 1079
Hpy8I GTNNAC 2 cut(s) 697, 946
HpyAV CCTTC 3 cut(s) 154, 386, 493
HpyCH4III ACNGT 1 cut(s) 78
HpyCH4V TGCA 9 cut(s) 98, 136, 178, 215, 313, 480, 523, 801, 941
HpyF10VI GCNNNNNNNGC 1 cut(s) 588
HpyF3I CTNAG 7 cut(s) 92, 231, 378, 722, 735, 975, 985
Hsp92II CATG 7 cut(s) 209, 343, 362, 404, 590, 805, 1027
HspAI GCGC 1 cut(s) 649
Ksp22I TGATCA 1 cut(s) 967
Kzo9I GATC 6 cut(s) 48, 298, 336, 527, 967, 1039
LmnI GCTCC 4 cut(s) 25, 64, 449, 1081
Lsp1109I GCAGC 1 cut(s) 603
LweI GCATC 5 cut(s) 202, 467, 773, 788, 1063
MaeI CTAG 5 cut(s) 12, 68, 446, 462, 1043
MalI GATC 6 cut(s) 50, 300, 338, 529, 969, 1041
MboI GATC 6 cut(s) 48, 298, 336, 527, 967, 1039
MboII GAAGA 4 cut(s) 416, 548, 651, 758
MfeI CAATTG 1 cut(s) 179
MflI RGATCY 1 cut(s) 1039
MlsI TGGCCA 2 cut(s) 344, 411
MluNI TGGCCA 2 cut(s) 344, 411
MlyI GAGTC 1 cut(s) 245
MmeI TCCRAC 1 cut(s) 737
MnlI CCTC 7 cut(s) 75, 160, 204, 240, 408, 717, 1009
Mox20I TGGCCA 2 cut(s) 344, 411
Mph1103I ATGCAT 1 cut(s) 803
MscI TGGCCA 2 cut(s) 344, 411
MslI CAYNNNNRTG 4 cut(s) 210, 405, 796, 941
Msp20I TGGCCA 2 cut(s) 344, 411
MspA1I CMGCKG 1 cut(s) 890
MunI CAATTG 1 cut(s) 179
MvnI CGCG 1 cut(s) 651
MwoI GCNNNNNNNGC 1 cut(s) 588
NdeII GATC 6 cut(s) 48, 298, 336, 527, 967, 1039
NheI GCTAGC 1 cut(s) 67
NlaIII CATG 7 cut(s) 209, 343, 362, 404, 590, 805, 1027
NlaIV GGNNCC 2 cut(s) 451, 915
NsiI ATGCAT 1 cut(s) 803
PagI TCATGA 1 cut(s) 400
PfeI GAWTC 2 cut(s) 896, 962
Pfl23II CGTACG 1 cut(s) 153
PflFI GACNNNGTC 1 cut(s) 359
PflMI CCANNNNNTGG 1 cut(s) 890
PkrI GCNGC 2 cut(s) 593, 889
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
PpuMI RGGWCCY 2 cut(s) 307, 395
Psp5II RGGWCCY 2 cut(s) 307, 395
PspFI CCCAGC 2 cut(s) 21, 504
PspLI CGTACG 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 451, 915
PspPI GGNCC 3 cut(s) 163, 307, 395
PspPPI RGGWCCY 2 cut(s) 307, 395
PstNI CAGNNNCTG 3 cut(s) 310, 890, 965
PsuI RGATCY 1 cut(s) 1039
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 4 cut(s) 210, 405, 796, 941
SatI GCNGC 2 cut(s) 592, 888
Sau3AI GATC 6 cut(s) 48, 298, 336, 527, 967, 1039
Sau96I GGNCC 3 cut(s) 163, 307, 395
SchI GAGTC 1 cut(s) 245
SfaNI GCATC 5 cut(s) 202, 467, 773, 788, 1063
SfcI CTRYAG 1 cut(s) 472
SinI GGWCC 2 cut(s) 307, 395
SmiMI CAYNNNNRTG 4 cut(s) 210, 405, 796, 941
SmlI CTYRAG 1 cut(s) 690
SmoI CTYRAG 1 cut(s) 690
SsiI CCGC 1 cut(s) 888
SspMI CTAG 5 cut(s) 12, 68, 446, 462, 1043
TaaI ACNGT 1 cut(s) 78
TaqI TCGA 2 cut(s) 241, 1053
TauI GCSGC 1 cut(s) 890
TfiI GAWTC 2 cut(s) 896, 962
TscAI CASTG 3 cut(s) 948, 979, 1041
TseI GCWGC 1 cut(s) 591
TspDTI ATGAA 4 cut(s) 99, 417, 549, 780
TspGWI ACGGA 1 cut(s) 759
TspRI CASTG 3 cut(s) 948, 979, 1041
Tth111I GACNNNGTC 1 cut(s) 359
Van91I CCANNNNNTGG 1 cut(s) 890
VpaK11BI GGWCC 2 cut(s) 307, 395
XapI RAATTY 3 cut(s) 739, 847, 1049
XspI CTAG 5 cut(s) 12, 68, 446, 462, 1043
Zsp2I ATGCAT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.