Rh7DG150000
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0003120
Physical Location & Seq
Forward (+)
12923647 .. 12925159
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG150000.1

Sequence Viewer

Length: 1080 bp
ATGGATACTGCTAGTAAGAGCTGGGAGCAGAAGTCATTAGTTGGGGAGATCATTCAAGGGATGGAGCTAGCACAACAGTTGAGGCTAAGTCTGAGTGCAGCATCAACTTCATCAGAAACTCGGGAGTTTTTAGTGCAAAGGATATTGACCTCGTACGAGAAGGCCCTTTTGCTACTGCAATTGAGTGGCACGCCTCAAACATCTCATGTGGATGCAAAATCCATTGTGCTTCAGGAATCTCGAATGTCTGCCAACACAAGTCCTTGTGGTGATGACTATAACCACAGGAGTTTCAAGGATCATCAGGACCTGCACGAAATCTCCAAAAAGAGAAAGATCATGGCCAAATGGACTGACCATGTCATAAGGGTTAGCTCTGAGAATGGGATTGAAGGACCTCATGAAGATGGCCATAGCTGGAGAAAATATGGGCAGAAAGATATTCTAGGAGCCAAATATCCCAGAAGCTACTATAGATGCACCTTCAGGAACACACAAAACTGCTGGGCTACAAAGCAAGTGCAGAGATCAGATGAAGACCCTACCATATTTGAAATCACATATAAAGGAAAGCATACCTGCTCTCATGGCAGCACATCAGTTCCACCACCACCATCACCAGAACAGCAAGAACAAAAAAGACATAAGCGCGAGAACATTTCTGAACCACAGCAGTCTCAAGTGAACCAAATGAGCTTTCCAACTAATCTGAGGGTTGATACTGAGAATTTGGAAGACAGAGACATGATGGCATCTCCGTTTGCTTTTACTTCAACTTCATTTGGATGCATGATGAATGGAGATGACTTCCTATCTCCGATGTTTGATAATGAGAATTTCTTTAGCAATTTCTCTCAACATTTGCTATCTCCAGCCACTGGCGAATCAAATTACCACTTGGCACCACCAAGCCAAATGAGAAACATTGCAGCGAACGGGCAACTTTCAGAATCTGATCACACTGAGATAATCTCAGCCACCAATTCCTCAACCAATTCTCCAATTCCAGACATGGATTTCCCACTGGATCTAGTGGAATTCGACCCCAATTTCCAATTTGACACTCCAGAACTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 WRKY

Protein Analysis

359

Amino Acids

40.73

Weight (kDa)

5.38

Isoelectric Point (pI)

54.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 135 - 194 1.1e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 318, 587
AccB1I GGYRCC 1 cut(s) 901
AccB7I CCANNNNNTGG 1 cut(s) 878
AccII CGCG 1 cut(s) 651
AclWI GGATC 2 cut(s) 306, 1035
AcoI YGGCCR 2 cut(s) 342, 409
AcsI RAATTY 3 cut(s) 727, 835, 1037
AcuI CTGAAG 2 cut(s) 215, 469
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 878
AgsI TTSAA 5 cut(s) 56, 295, 392, 554, 774
AjuI GAANNNNNNNTTGG 2 cut(s) 681, 713
AluBI AGCT 6 cut(s) 21, 67, 375, 417, 468, 696
AluI AGCT 6 cut(s) 21, 67, 375, 417, 468, 696
Alw26I GTCTC 2 cut(s) 681, 735
AlwI GGATC 2 cut(s) 306, 1035
AlwNI CAGNNNCTG 3 cut(s) 310, 878, 953
Ama87I CYCGRG 1 cut(s) 120
AoxI GGCC 3 cut(s) 162, 342, 409
ApeKI GCWGC 3 cut(s) 98, 591, 929
ApoI RAATTY 3 cut(s) 727, 835, 1037
AspLEI GCGC 1 cut(s) 651
AspS9I GGNCC 3 cut(s) 163, 307, 395
AsuHPI GGTGA 2 cut(s) 281, 609
AsuNHI GCTAGC 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 2 cut(s) 307, 395
BalI TGGCCA 2 cut(s) 344, 411
BanI GGYRCC 1 cut(s) 901
BbsI GAAGAC 2 cut(s) 543, 741
BbvI GCAGC 3 cut(s) 110, 603, 941
BccI CCATC 4 cut(s) 55, 401, 622, 742
BclI TGATCA 1 cut(s) 955
BcoDI GTCTC 2 cut(s) 681, 735
BfaI CTAG 4 cut(s) 12, 68, 446, 1031
BfmI CTRYAG 1 cut(s) 472
BfuAI ACCTGC 2 cut(s) 318, 587
BisI GCNGC 3 cut(s) 99, 592, 930
BlsI GCNGC 3 cut(s) 100, 593, 931
Bme18I GGWCC 2 cut(s) 307, 395
BmeT110I CYCGRG 1 cut(s) 120
BmgT120I GGNCC 3 cut(s) 163, 307, 395
BmiI GGNNCC 2 cut(s) 451, 903
BmsI GCATC 5 cut(s) 110, 202, 467, 761, 776
BmtI GCTAGC 1 cut(s) 71
BpiI GAAGAC 2 cut(s) 543, 741
BplI GAGNNNNNCTC 2 cut(s) 956, 988
BpmI CTGGAG 3 cut(s) 439, 855, 1050
BpuEI CTTGAG 1 cut(s) 663
BsaXI ACNNNNNCTCC 6 cut(s) 17, 47, 305, 335, 982, 1012
Bsc4I CCNNNNNNNGG 1 cut(s) 878
Bse1I ACTGG 2 cut(s) 883, 1029
Bse3DI GCAATG 1 cut(s) 924
BseGI GGATG 3 cut(s) 66, 217, 791
BseLI CCNNNNNNNGG 1 cut(s) 878
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 6 cut(s) 83, 369, 701, 714, 954, 987
BseNI ACTGG 2 cut(s) 883, 1029
BseXI GCAGC 3 cut(s) 110, 603, 941
BseYI CCCAGC 2 cut(s) 21, 504
BsgI GTGCAG 3 cut(s) 117, 296, 542
Bsh1236I CGCG 1 cut(s) 651
BshFI GGCC 3 cut(s) 164, 344, 411
BshNI GGYRCC 1 cut(s) 901
BsiHKCI CYCGRG 1 cut(s) 120
BsiWI CGTACG 1 cut(s) 153
BslI CCNNNNNNNGG 1 cut(s) 878
BsmAI GTCTC 2 cut(s) 681, 735
BsnI GGCC 3 cut(s) 164, 344, 411
BsoBI CYCGRG 1 cut(s) 120
Bsp143I GATC 6 cut(s) 48, 298, 336, 527, 955, 1027
BspANI GGCC 3 cut(s) 164, 344, 411
BspCNI CTCAG 6 cut(s) 84, 370, 702, 715, 955, 986
BspFNI CGCG 1 cut(s) 651
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 451, 903
BspMI ACCTGC 2 cut(s) 318, 587
BspOI GCTAGC 1 cut(s) 71
BspPI GGATC 2 cut(s) 306, 1035
BspT107I GGYRCC 1 cut(s) 901
BsrDI GCAATG 1 cut(s) 924
BsrI ACTGG 2 cut(s) 883, 1029
BssMI GATC 6 cut(s) 48, 298, 336, 527, 955, 1027
Bst4CI ACNGT 1 cut(s) 78
BstC8I GCNNGC 2 cut(s) 69, 191
BstDEI CTNAG 7 cut(s) 86, 92, 378, 710, 723, 963, 973
BstF5I GGATG 3 cut(s) 66, 217, 791
BstFNI CGCG 1 cut(s) 651
BstHHI GCGC 1 cut(s) 651
BstKTI GATC 6 cut(s) 51, 301, 339, 530, 958, 1030
BstMAI GTCTC 2 cut(s) 681, 735
BstMBI GATC 6 cut(s) 48, 298, 336, 527, 955, 1027
BstMWI GCNNNNNNNGC 1 cut(s) 588
BstSFI CTRYAG 1 cut(s) 472
BstUI CGCG 1 cut(s) 651
BstV1I GCAGC 3 cut(s) 110, 603, 941
BstV2I GAAGAC 2 cut(s) 543, 741
BstX2I RGATCY 1 cut(s) 1027
BstYI RGATCY 1 cut(s) 1027
BsuRI GGCC 3 cut(s) 164, 344, 411
BtsCI GGATG 3 cut(s) 66, 217, 791
BtsIMutI CAGTG 3 cut(s) 876, 960, 1022
BveI ACCTGC 2 cut(s) 318, 587
Cac8I GCNNGC 2 cut(s) 69, 191
CaiI CAGNNNCTG 3 cut(s) 310, 878, 953
CciI TCATGA 1 cut(s) 400
CfoI GCGC 1 cut(s) 651
Cfr13I GGNCC 3 cut(s) 163, 307, 395
Csp6I GTAC 1 cut(s) 154
CviAII CATG 8 cut(s) 206, 340, 359, 401, 587, 745, 790, 1012
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 7 cut(s) 86, 92, 378, 710, 723, 963, 973
DpnI GATC 6 cut(s) 50, 300, 338, 529, 957, 1029
DpnII GATC 6 cut(s) 48, 298, 336, 527, 955, 1027
EaeI YGGCCR 2 cut(s) 342, 409
Eco47I GGWCC 2 cut(s) 307, 395
Eco57I CTGAAG 2 cut(s) 215, 469
Eco88I CYCGRG 1 cut(s) 120
EcoO109I RGGNCCY 3 cut(s) 163, 307, 395
EcoRI GAATTC 1 cut(s) 1037
EcoT22I ATGCAT 1 cut(s) 791
FaeI CATG 8 cut(s) 209, 343, 362, 404, 590, 748, 793, 1015
FatI CATG 8 cut(s) 205, 339, 358, 400, 586, 744, 789, 1011
FbaI TGATCA 1 cut(s) 955
Fnu4HI GCNGC 3 cut(s) 99, 592, 930
FokI GGATG 3 cut(s) 73, 224, 798
Fsp4HI GCNGC 3 cut(s) 99, 592, 930
FspBI CTAG 4 cut(s) 12, 68, 446, 1031
GlaI GCGC 1 cut(s) 650
GluI GCNGC 3 cut(s) 99, 592, 930
GsaI CCCAGC 2 cut(s) 25, 508
GsuI CTGGAG 3 cut(s) 439, 855, 1050
HaeIII GGCC 3 cut(s) 164, 344, 411
HhaI GCGC 1 cut(s) 651
Hin1II CATG 8 cut(s) 209, 343, 362, 404, 590, 748, 793, 1015
Hin6I GCGC 1 cut(s) 649
HinP1I GCGC 1 cut(s) 649
HinfI GANTC 3 cut(s) 236, 884, 950
HphI GGTGA 2 cut(s) 281, 609
Hpy166II GTNNAC 1 cut(s) 685
Hpy188III TCNNGA 8 cut(s) 122, 233, 240, 305, 401, 487, 1007, 1067
Hpy8I GTNNAC 1 cut(s) 685
HpyAV CCTTC 3 cut(s) 154, 386, 493
HpyCH4III ACNGT 1 cut(s) 78
HpyCH4V TGCA 9 cut(s) 98, 136, 178, 215, 313, 480, 523, 789, 929
HpyF10VI GCNNNNNNNGC 1 cut(s) 588
HpyF3I CTNAG 7 cut(s) 86, 92, 378, 710, 723, 963, 973
Hsp92II CATG 8 cut(s) 209, 343, 362, 404, 590, 748, 793, 1015
HspAI GCGC 1 cut(s) 649
Ksp22I TGATCA 1 cut(s) 955
Kzo9I GATC 6 cut(s) 48, 298, 336, 527, 955, 1027
LmnI GCTCC 3 cut(s) 25, 64, 449
Lsp1109I GCAGC 3 cut(s) 110, 603, 941
LweI GCATC 5 cut(s) 110, 202, 467, 761, 776
MaeI CTAG 4 cut(s) 12, 68, 446, 1031
MalI GATC 6 cut(s) 50, 300, 338, 529, 957, 1029
MboI GATC 6 cut(s) 48, 298, 336, 527, 955, 1027
MboII GAAGA 3 cut(s) 416, 548, 746
MfeI CAATTG 1 cut(s) 179
MflI RGATCY 1 cut(s) 1027
MlsI TGGCCA 2 cut(s) 344, 411
MluNI TGGCCA 2 cut(s) 344, 411
MmeI TCCRAC 1 cut(s) 725
MnlI CCTC 6 cut(s) 75, 160, 204, 408, 705, 997
Mox20I TGGCCA 2 cut(s) 344, 411
Mph1103I ATGCAT 1 cut(s) 791
MscI TGGCCA 2 cut(s) 344, 411
MslI CAYNNNNRTG 3 cut(s) 210, 405, 784
Msp20I TGGCCA 2 cut(s) 344, 411
MunI CAATTG 1 cut(s) 179
MvnI CGCG 1 cut(s) 651
MwoI GCNNNNNNNGC 1 cut(s) 588
NdeII GATC 6 cut(s) 48, 298, 336, 527, 955, 1027
NheI GCTAGC 1 cut(s) 67
NlaIII CATG 8 cut(s) 209, 343, 362, 404, 590, 748, 793, 1015
NlaIV GGNNCC 2 cut(s) 451, 903
NsiI ATGCAT 1 cut(s) 791
PagI TCATGA 1 cut(s) 400
PfeI GAWTC 3 cut(s) 236, 884, 950
Pfl23II CGTACG 1 cut(s) 153
PflFI GACNNNGTC 1 cut(s) 359
PflMI CCANNNNNTGG 1 cut(s) 878
PkrI GCNGC 3 cut(s) 100, 593, 931
PpuMI RGGWCCY 2 cut(s) 307, 395
Psp5II RGGWCCY 2 cut(s) 307, 395
PspFI CCCAGC 2 cut(s) 21, 504
PspLI CGTACG 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 451, 903
PspPI GGNCC 3 cut(s) 163, 307, 395
PspPPI RGGWCCY 2 cut(s) 307, 395
PstNI CAGNNNCTG 3 cut(s) 310, 878, 953
PsuI RGATCY 1 cut(s) 1027
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 3 cut(s) 210, 405, 784
SatI GCNGC 3 cut(s) 99, 592, 930
Sau3AI GATC 6 cut(s) 48, 298, 336, 527, 955, 1027
Sau96I GGNCC 3 cut(s) 163, 307, 395
SfaNI GCATC 5 cut(s) 110, 202, 467, 761, 776
SfcI CTRYAG 1 cut(s) 472
SinI GGWCC 2 cut(s) 307, 395
SmiMI CAYNNNNRTG 3 cut(s) 210, 405, 784
SmlI CTYRAG 1 cut(s) 678
SmoI CTYRAG 1 cut(s) 678
SspMI CTAG 4 cut(s) 12, 68, 446, 1031
TaaI ACNGT 1 cut(s) 78
TaqI TCGA 2 cut(s) 241, 1041
TfiI GAWTC 3 cut(s) 236, 884, 950
TscAI CASTG 3 cut(s) 883, 967, 1029
TseI GCWGC 3 cut(s) 98, 591, 929
TspDTI ATGAA 5 cut(s) 99, 417, 549, 768, 809
TspGWI ACGGA 1 cut(s) 747
TspRI CASTG 3 cut(s) 883, 967, 1029
Tth111I GACNNNGTC 1 cut(s) 359
Van91I CCANNNNNTGG 1 cut(s) 878
VpaK11BI GGWCC 2 cut(s) 307, 395
XapI RAATTY 3 cut(s) 727, 835, 1037
XspI CTAG 4 cut(s) 12, 68, 446, 1031
Zsp2I ATGCAT 1 cut(s) 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.