MD06G1108000.v1.1

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005446
Physical Location & Seq
Forward (+)
24762729 .. 24763094
366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1108000.v1.1.491

Sequence Viewer

Length: 366 bp
ATGGCTCATCCACCTCGCTATATTGGGTTTGCCAGTGTAATTATCTTGCTTTGTGCAATATTATTAGCAGCAAAGGCTCAGACGGCCTTGAACTGTGCGACTGTGTTTCACGAGTTCAGCTTTTGCAAGTCGTTTGCCCCGGGATTGTCTTCTGAACCGTTGAAGCAGTGCTGTGACTCCGTCAAGACTTTGAACACGATTGCCAGACATGATGAAGGTGGTCCACAAATGATCTGCGAATGCATCGAGAACTTGTCTTATTGGAGCAACGTCTCTTTCGACACCTCCCGCATCCAAGATCTTCCAACCAAATGCCAACTCCACTTGAGTTTTCTCATTTCTAATAGCATGGATTGCTTCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

122

Amino Acids

13.42

Weight (kDa)

6.38

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 32 - 119 1e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 343
AgsI TTSAA 3 cut(s) 91, 163, 193
AluBI AGCT 1 cut(s) 120
AluI AGCT 1 cut(s) 120
Alw26I GTCTC 1 cut(s) 277
Ama87I CYCGRG 1 cut(s) 139
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 1 cut(s) 68
AspS9I GGNCC 1 cut(s) 221
AsuC2I CCSGG 2 cut(s) 140, 141
AvaI CYCGRG 1 cut(s) 139
AvaII GGWCC 1 cut(s) 221
BauI CACGAG 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 80
BceAI ACGGC 1 cut(s) 99
BcnI CCSGG 2 cut(s) 140, 141
BcoDI GTCTC 1 cut(s) 277
BglII AGATCT 1 cut(s) 298
BisI GCNGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 2 cut(s) 140, 141
Bme18I GGWCC 1 cut(s) 221
BmeT110I CYCGRG 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 221
BmrFI CCNGG 2 cut(s) 140, 141
BmsI GCATC 2 cut(s) 252, 300
BpiI GAAGAC 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 346
BpuMI CCSGG 2 cut(s) 140, 141
BsaJI CCNNGG 2 cut(s) 138, 139
Bse1I ACTGG 1 cut(s) 33
BseDI CCNNGG 2 cut(s) 138, 139
BseGI GGATG 2 cut(s) 7, 291
BseMII CTCAG 1 cut(s) 92
BseNI ACTGG 1 cut(s) 33
BseXI GCAGC 1 cut(s) 80
BshFI GGCC 1 cut(s) 86
BsiHKCI CYCGRG 1 cut(s) 139
BsiSI CCGG 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 277
BsmBI CGTCTC 1 cut(s) 277
BsmI GAATGC 1 cut(s) 245
BsnI GGCC 1 cut(s) 86
BsoBI CYCGRG 1 cut(s) 139
Bsp143I GATC 2 cut(s) 231, 298
BspACI CCGC 1 cut(s) 289
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 1 cut(s) 91
BsrI ACTGG 1 cut(s) 33
BssECI CCNNGG 2 cut(s) 138, 139
BssMI GATC 2 cut(s) 231, 298
BssSI CACGAG 1 cut(s) 110
Bst2BI CACGAG 1 cut(s) 110
Bst4CI ACNGT 3 cut(s) 95, 103, 159
BstAPI GCANNNNNTGC 1 cut(s) 354
BstDEI CTNAG 1 cut(s) 78
BstF5I GGATG 2 cut(s) 7, 291
BstKTI GATC 2 cut(s) 234, 301
BstMAI GTCTC 1 cut(s) 277
BstMBI GATC 2 cut(s) 231, 298
BstMWI GCNNNNNNNGC 3 cut(s) 74, 83, 354
BstSCI CCNGG 2 cut(s) 138, 139
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 1 cut(s) 86
BtsCI GGATG 2 cut(s) 7, 291
BtsI GCAGTG 1 cut(s) 173
BtsIMutI CAGTG 2 cut(s) 40, 173
Cfr13I GGNCC 1 cut(s) 221
Cfr9I CCCGGG 1 cut(s) 139
CviAII CATG 2 cut(s) 209, 349
CviJI RGCY 4 cut(s) 5, 77, 86, 120
CviKI_1 RGCY 4 cut(s) 5, 77, 86, 120
DdeI CTNAG 1 cut(s) 78
DpnI GATC 2 cut(s) 233, 300
DpnII GATC 2 cut(s) 231, 298
Eco47I GGWCC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 343
Eco88I CYCGRG 1 cut(s) 139
EcoT22I ATGCAT 1 cut(s) 245
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 2 cut(s) 212, 352
FaiI YATR 3 cut(s) 21, 210, 350
FatI CATG 2 cut(s) 208, 348
FauI CCCGC 1 cut(s) 296
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 1 cut(s) 278
Fsp4HI GCNGC 1 cut(s) 69
GluI GCNGC 1 cut(s) 69
HaeIII GGCC 1 cut(s) 86
HapII CCGG 1 cut(s) 140
Hin1II CATG 2 cut(s) 212, 352
HinfI GANTC 1 cut(s) 176
HpaII CCGG 1 cut(s) 140
Hpy166II GTNNAC 1 cut(s) 224
Hpy188I TCNGA 2 cut(s) 81, 154
Hpy188III TCNNGA 3 cut(s) 110, 184, 247
Hpy8I GTNNAC 1 cut(s) 224
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 3 cut(s) 95, 103, 159
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 3 cut(s) 56, 126, 243
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 83, 354
HpyF3I CTNAG 1 cut(s) 78
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 2 cut(s) 212, 352
Kzo9I GATC 2 cut(s) 231, 298
LmnI GCTCC 1 cut(s) 264
LpnPI CCDG 4 cut(s) 46, 153, 217, 346
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 2 cut(s) 252, 300
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 173
MalI GATC 2 cut(s) 233, 300
MboI GATC 2 cut(s) 231, 298
MboII GAAGA 2 cut(s) 141, 293
MflI RGATCY 1 cut(s) 298
MluCI AATT 1 cut(s) 39
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 2 cut(s) 24, 295
Mph1103I ATGCAT 1 cut(s) 245
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 2 cut(s) 140, 141
Mva1269I GAATGC 1 cut(s) 245
MwoI GCNNNNNNNGC 3 cut(s) 74, 83, 354
NciI CCSGG 2 cut(s) 140, 141
NdeII GATC 2 cut(s) 231, 298
NlaIII CATG 2 cut(s) 212, 352
NmuCI GTSAC 1 cut(s) 173
NsiI ATGCAT 1 cut(s) 245
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 245
PflFI GACNNNGTC 1 cut(s) 179
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PspPI GGNCC 1 cut(s) 221
PsuI RGATCY 1 cut(s) 298
PsyI GACNNNGTC 1 cut(s) 179
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 2 cut(s) 231, 298
Sau96I GGNCC 1 cut(s) 221
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 2 cut(s) 140, 141
SetI ASST 6 cut(s) 16, 122, 220, 273, 287, 365
SfaNI GCATC 2 cut(s) 252, 300
SinI GGWCC 1 cut(s) 221
SmaI CCCGGG 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 325
SmoI CTYRAG 1 cut(s) 325
Sse9I AATT 1 cut(s) 39
SsiI CCGC 1 cut(s) 289
SspI AATATT 1 cut(s) 60
StyD4I CCNGG 2 cut(s) 138, 139
TaaI ACNGT 3 cut(s) 95, 103, 159
TaiI ACGT 1 cut(s) 273
TaqI TCGA 2 cut(s) 246, 279
TasI AATT 1 cut(s) 39
TscAI CASTG 2 cut(s) 40, 173
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 1 cut(s) 228
TspGWI ACGGA 1 cut(s) 169
TspMI CCCGGG 1 cut(s) 139
TspRI CASTG 2 cut(s) 40, 173
Tth111I GACNNNGTC 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 221
XmaI CCCGGG 1 cut(s) 139
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.