Rroxscaffold_2G00116550

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005446
Physical Location & Seq
Reverse (-)
43787155 .. 43788564
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00116550.1

Sequence Viewer

Length: 480 bp
ATGGCTGCTCGTCCTTTTTGTTATGTCTGTGTAACAAGTCTTCTTGTGCTTGTTCCTGTGCTGCTGCTTTGTTCATCATTAGGGAAGGCTCAGACGACGGAATACTGCACGAATGTGTTCGAGGAATTTAGCTCTTGCCTGAGTTTTGTCGGGGAGTTATCTCCTGAACCGACGGCGGCTTGCTGTGGTTCCCTGACAAGTTTGAATTCGTTAGCACAACAAGAGGAGAGTGGTCCGTGGATGATATGTGAGTGCATTGAGAGCTCGTCTTATTGGACCAGAATCCCTTTCAGTGCCTCCCGAATTCAGGAGCTCCCTACATCATGCCAACTCCATCTTAGTTTCCCCATTTCTAATAGCATGGATTGCTCCAAAAGAATAGAAGGAATTACTGCAATTGGTTCCTCTGTTCATCACAAGCACATTATGCCACCAGCAATTTGGCTTTGCGCTCTATGGTGTGAAAAGGATGTCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

159

Amino Acids

17.46

Weight (kDa)

5.0

Isoelectric Point (pI)

70.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 36 - 123 3.5e-06 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 176
AcsI RAATTY 3 cut(s) 125, 205, 303
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 1 cut(s) 205
AleI CACNNNNGTG 1 cut(s) 113
AluBI AGCT 3 cut(s) 132, 264, 313
AluI AGCT 3 cut(s) 132, 264, 313
Alw21I GWGCWC 2 cut(s) 266, 315
ApeKI GCWGC 3 cut(s) 5, 61, 64
ApoI RAATTY 3 cut(s) 125, 205, 303
Asp700I GAANNNNTTC 1 cut(s) 116
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 2 cut(s) 233, 276
AvaII GGWCC 2 cut(s) 233, 276
BanII GRGCYC 2 cut(s) 266, 315
BarI GAAGNNNNNNTAC 2 cut(s) 24, 56
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 2 cut(s) 266, 315
BbvI GCAGC 2 cut(s) 48, 51
BccI CCATC 1 cut(s) 342
BceAI ACGGC 1 cut(s) 189
BisI GCNGC 4 cut(s) 6, 62, 65, 177
BlsI GCNGC 4 cut(s) 7, 63, 66, 178
Bme18I GGWCC 2 cut(s) 233, 276
BmgT120I GGNCC 2 cut(s) 233, 276
BmiI GGNNCC 2 cut(s) 190, 403
BpiI GAAGAC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 236
Bsc4I CCNNNNNNNGG 1 cut(s) 307
BseDI CCNNGG 1 cut(s) 236
BseGI GGATG 2 cut(s) 246, 475
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 2 cut(s) 104, 131
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 2 cut(s) 48, 51
BsgI GTGCAG 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 266, 315
BslI CCNNNNNNNGG 1 cut(s) 307
Bsp1286I GDGCHC 2 cut(s) 266, 315
BspACI CCGC 1 cut(s) 176
BspCNI CTCAG 2 cut(s) 103, 132
BspLI GGNNCC 2 cut(s) 190, 403
BssECI CCNNGG 1 cut(s) 236
BstAPI GCANNNNNTGC 2 cut(s) 366, 427
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 3 cut(s) 90, 140, 338
BstDSI CCRYGG 1 cut(s) 236
BstF5I GGATG 2 cut(s) 246, 475
BstHHI GCGC 1 cut(s) 452
BstMWI GCNNNNNNNGC 3 cut(s) 261, 366, 427
BstV1I GCAGC 2 cut(s) 48, 51
BstV2I GAAGAC 1 cut(s) 32
BstXI CCANNNNNNTGG 1 cut(s) 441
BtgI CCRYGG 1 cut(s) 236
BtsCI GGATG 2 cut(s) 246, 475
BtsIMutI CAGTG 1 cut(s) 298
Cac8I GCNNGC 1 cut(s) 181
CfoI GCGC 1 cut(s) 452
Cfr13I GGNCC 2 cut(s) 233, 276
CviAII CATG 2 cut(s) 324, 361
CviJI RGCY 7 cut(s) 5, 89, 132, 179, 264, 313, 445
CviKI_1 RGCY 7 cut(s) 5, 89, 132, 179, 264, 313, 445
DdeI CTNAG 3 cut(s) 90, 140, 338
Ecl136II GAGCTC 2 cut(s) 264, 313
Eco24I GRGCYC 2 cut(s) 266, 315
Eco47I GGWCC 2 cut(s) 233, 276
Eco53kI GAGCTC 2 cut(s) 264, 313
EcoICRI GAGCTC 2 cut(s) 264, 313
EcoRI GAATTC 2 cut(s) 205, 303
EcoT38I GRGCYC 2 cut(s) 266, 315
FaeI CATG 2 cut(s) 327, 364
FaiI YATR 6 cut(s) 24, 247, 325, 362, 428, 457
FatI CATG 2 cut(s) 323, 360
Fnu4HI GCNGC 4 cut(s) 6, 62, 65, 177
FokI GGATG 1 cut(s) 253
FriOI GRGCYC 2 cut(s) 266, 315
Fsp4HI GCNGC 4 cut(s) 6, 62, 65, 177
GlaI GCGC 1 cut(s) 451
GluI GCNGC 4 cut(s) 6, 62, 65, 177
HhaI GCGC 1 cut(s) 452
Hin1II CATG 2 cut(s) 327, 364
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HinfI GANTC 1 cut(s) 282
Hpy188I TCNGA 1 cut(s) 93
Hpy188III TCNNGA 3 cut(s) 164, 300, 308
Hpy99I CGWCG 2 cut(s) 100, 175
HpyAV CCTTC 2 cut(s) 79, 377
HpyCH4V TGCA 3 cut(s) 108, 255, 395
HpyF10VI GCNNNNNNNGC 3 cut(s) 261, 366, 427
HpyF3I CTNAG 3 cut(s) 90, 140, 338
Hsp92II CATG 2 cut(s) 327, 364
HspAI GCGC 1 cut(s) 450
LmnI GCTCC 3 cut(s) 310, 318, 374
LpnPI CCDG 7 cut(s) 69, 152, 177, 206, 292, 293, 447
Lsp1109I GCAGC 2 cut(s) 48, 51
MaeIII GTNAC 1 cut(s) 31
MboII GAAGA 1 cut(s) 32
MfeI CAATTG 1 cut(s) 396
MhlI GDGCHC 2 cut(s) 266, 315
MluCI AATT 6 cut(s) 125, 205, 303, 387, 396, 438
MnlI CCTC 4 cut(s) 115, 217, 307, 415
MroXI GAANNNNTTC 1 cut(s) 116
MslI CAYNNNNRTG 1 cut(s) 113
MunI CAATTG 1 cut(s) 396
MwoI GCNNNNNNNGC 3 cut(s) 261, 366, 427
NlaIII CATG 2 cut(s) 327, 364
NlaIV GGNNCC 2 cut(s) 190, 403
OliI CACNNNNGTG 1 cut(s) 113
PdmI GAANNNNTTC 1 cut(s) 116
PfeI GAWTC 1 cut(s) 282
PkrI GCNGC 4 cut(s) 7, 63, 66, 178
Psp124BI GAGCTC 2 cut(s) 266, 315
PspN4I GGNNCC 2 cut(s) 190, 403
PspPI GGNCC 2 cut(s) 233, 276
RseI CAYNNNNRTG 1 cut(s) 113
SacI GAGCTC 2 cut(s) 266, 315
SatI GCNGC 4 cut(s) 6, 62, 65, 177
Sau96I GGNCC 2 cut(s) 233, 276
SduI GDGCHC 2 cut(s) 266, 315
SetI ASST 3 cut(s) 134, 266, 315
SinI GGWCC 2 cut(s) 233, 276
SmiMI CAYNNNNRTG 1 cut(s) 113
Sse9I AATT 6 cut(s) 125, 205, 303, 387, 396, 438
SsiI CCGC 1 cut(s) 176
SstI GAGCTC 2 cut(s) 266, 315
TaqI TCGA 2 cut(s) 120, 474
TasI AATT 6 cut(s) 125, 205, 303, 387, 396, 438
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 1 cut(s) 282
TscAI CASTG 1 cut(s) 298
TseI GCWGC 3 cut(s) 5, 61, 64
TspDTI ATGAA 2 cut(s) 63, 401
TspGWI ACGGA 2 cut(s) 113, 225
TspRI CASTG 1 cut(s) 298
VpaK11BI GGWCC 2 cut(s) 233, 276
XapI RAATTY 3 cut(s) 125, 205, 303
XcmI CCANNNNNNNNNTGG 1 cut(s) 438
XmnI GAANNNNTTC 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.