Rh2CG312400

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0005446
Physical Location & Seq
Forward (+)
39471637 .. 39472113
477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG312400.1

Sequence Viewer

Length: 300 bp
ATGGGTCAAGCGTCCAGTTTCTGTGCTCAAGCCTGGAACGAGTTCAGCAACTGCATGAGGTTCATCACCGGGTTATTTTCGCAACCGTCAACGCAGTGCTGTGATTCGGTGAGGAGATTGAACAAGATGGCCAAAAGTAGTGAGAACGGACCTCGCGATATATGTCAATGCATTGAGGACATGTCTCGTACATATGGTATTCCTTTTGTAGCCTCCATCATCCAAGATCTACCCATCATATGCAATGCGCACCTTAGTTTCCCAATCTCTAATCGCATGAACTGCACCATGCTCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

99

Amino Acids

11.07

Weight (kDa)

7.52

Isoelectric Point (pI)

56.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 8 - 95 1.1e-06 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 249
AccII CGCG 1 cut(s) 156
AcoI YGGCCR 1 cut(s) 129
AfaI GTAC 1 cut(s) 190
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 1 cut(s) 121
AjnI CCWGG 1 cut(s) 32
AluBI AGCT 1 cut(s) 297
AluI AGCT 1 cut(s) 297
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 1 cut(s) 189
AlwNI CAGNNNCTG 2 cut(s) 21, 51
AoxI GGCC 1 cut(s) 129
Asp700I GAANNNNTTC 1 cut(s) 41
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 149
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 58, 121
AvaII GGWCC 1 cut(s) 149
BalI TGGCCA 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 28
BccI CCATC 3 cut(s) 121, 224, 242
BciT130I CCWGG 1 cut(s) 34
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 1 cut(s) 189
BglII AGATCT 1 cut(s) 226
BlpI GCTNAGC 1 cut(s) 293
Bme1390I CCNGG 2 cut(s) 34, 70
Bme18I GGWCC 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 149
BmrFI CCNGG 2 cut(s) 34, 70
Bpu1102I GCTNAGC 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 12
BpuMI CCSGG 1 cut(s) 70
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 250
BseBI CCWGG 1 cut(s) 34
BseGI GGATG 1 cut(s) 219
BseMI GCAATG 1 cut(s) 250
BseNI ACTGG 1 cut(s) 15
BseRI GAGGAG 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 268
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 1 cut(s) 131
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 1 cut(s) 69
BsmAI GTCTC 1 cut(s) 189
BsnI GGCC 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 28
Bsp143I GATC 1 cut(s) 226
Bsp1720I GCTNAGC 1 cut(s) 293
Bsp68I TCGCGA 1 cut(s) 156
BspANI GGCC 1 cut(s) 131
BspFNI CGCG 1 cut(s) 156
BsrDI GCAATG 1 cut(s) 250
BsrI ACTGG 1 cut(s) 15
BssMI GATC 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 34
Bst4CI ACNGT 1 cut(s) 87
BstAPI GCANNNNNTGC 1 cut(s) 282
BstDEI CTNAG 2 cut(s) 254, 293
BstF5I GGATG 1 cut(s) 219
BstFNI CGCG 1 cut(s) 156
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 1 cut(s) 229
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 1 cut(s) 226
BstMWI GCNNNNNNNGC 1 cut(s) 282
BstNI CCWGG 1 cut(s) 34
BstNSI RCATGY 1 cut(s) 184
BstSCI CCNGG 2 cut(s) 32, 68
BstUI CGCG 1 cut(s) 156
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 1 cut(s) 131
BtsCI GGATG 1 cut(s) 219
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 101
BtuMI TCGCGA 1 cut(s) 156
CaiI CAGNNNCTG 2 cut(s) 21, 51
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 149
Csp6I GTAC 1 cut(s) 189
CviAII CATG 4 cut(s) 55, 181, 277, 289
CviJI RGCY 4 cut(s) 32, 131, 212, 297
CviKI_1 RGCY 4 cut(s) 32, 131, 212, 297
CviQI GTAC 1 cut(s) 189
DdeI CTNAG 2 cut(s) 254, 293
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 32
EcoT22I ATGCAT 1 cut(s) 173
FaeI CATG 4 cut(s) 58, 184, 280, 292
FatI CATG 4 cut(s) 54, 180, 276, 288
FauNDI CATATG 2 cut(s) 193, 239
FokI GGATG 1 cut(s) 206
FspAI RTGCGCAY 1 cut(s) 249
FspI TGCGCA 1 cut(s) 249
GlaI GCGC 1 cut(s) 249
HaeIII GGCC 1 cut(s) 131
HapII CCGG 1 cut(s) 69
HhaI GCGC 1 cut(s) 250
Hin1II CATG 4 cut(s) 58, 184, 280, 292
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HinfI GANTC 1 cut(s) 104
HpaII CCGG 1 cut(s) 69
HphI GGTGA 2 cut(s) 58, 121
Hpy166II GTNNAC 1 cut(s) 90
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 1 cut(s) 90
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4V TGCA 4 cut(s) 54, 171, 243, 285
HpyF10VI GCNNNNNNNGC 1 cut(s) 282
HpyF3I CTNAG 2 cut(s) 254, 293
Hsp92II CATG 4 cut(s) 58, 184, 280, 292
HspAI GCGC 1 cut(s) 248
Kzo9I GATC 1 cut(s) 226
LpnPI CCDG 4 cut(s) 19, 28, 46, 82
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 1 cut(s) 28
MlsI TGGCCA 1 cut(s) 131
MluNI TGGCCA 1 cut(s) 131
MnlI CCTC 5 cut(s) 51, 105, 162, 169, 223
Mox20I TGGCCA 1 cut(s) 131
Mph1103I ATGCAT 1 cut(s) 173
MroXI GAANNNNTTC 1 cut(s) 41
MscI TGGCCA 1 cut(s) 131
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 297
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 2 cut(s) 34, 70
MvaI CCWGG 1 cut(s) 34
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 1 cut(s) 282
NciI CCSGG 1 cut(s) 70
NdeI CATATG 2 cut(s) 193, 239
NdeII GATC 1 cut(s) 226
NlaIII CATG 4 cut(s) 58, 184, 280, 292
NruI TCGCGA 1 cut(s) 156
NsbI TGCGCA 1 cut(s) 249
NsiI ATGCAT 1 cut(s) 173
NspI RCATGY 1 cut(s) 184
PciI ACATGT 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 1 cut(s) 104
PscI ACATGT 1 cut(s) 180
Psp6I CCWGG 1 cut(s) 32
PspGI CCWGG 1 cut(s) 32
PspPI GGNCC 1 cut(s) 149
PstNI CAGNNNCTG 2 cut(s) 21, 51
PsuI RGATCY 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 297
RruI TCGCGA 1 cut(s) 156
RsaI GTAC 1 cut(s) 190
RsaNI GTAC 1 cut(s) 189
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 1 cut(s) 149
ScrFI CCNGG 2 cut(s) 34, 70
SduI GDGCHC 1 cut(s) 28
SetI ASST 4 cut(s) 62, 154, 255, 299
SinI GGWCC 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
StyD4I CCNGG 2 cut(s) 32, 68
TaaI ACNGT 1 cut(s) 87
TfiI GAWTC 1 cut(s) 104
TscAI CASTG 1 cut(s) 101
TspDTI ATGAA 2 cut(s) 52, 293
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 1 cut(s) 101
VpaK11BI GGWCC 1 cut(s) 149
XceI RCATGY 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 41
Zsp2I ATGCAT 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.