MD17G1235700.v1.1

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005446
Physical Location & Seq
Reverse (-)
28468141 .. 28468494
354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1235700.v1.1.491

Sequence Viewer

Length: 354 bp
ATGGCGCCTCGCCTTTTGGCTTATGTAGCCATGTTTCTGCTAGTTTGTGGATCTGCCAAGGCATCGCACGCGTCCAGTTTCTGTGTTAGTGTGTGGTATGAGTTGGGCAATTGCCTGAATTTTCTTACTGGGTTTTATGCTGACCCTACGCTCGAATGCTGTAACTCCGTCCGGACGTTGAACACGATGGCCAAAAGTGATGAGGCTGAACCTCAAAGTATTTGTGAATGCATTGAGGGCGTGGCTGATGCGTATCGAATTCGTTTCGTAGCGTCCCTCATCCAAGATCTTCCCATCAAATGCAATGCTCATCTTAGTTTCCCTATTTCTAATAGCATGGATTGCACCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

118

Amino Acids

12.81

Weight (kDa)

5.19

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 28 - 115 1e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 71
AccIII TCCGGA 1 cut(s) 171
AclWI GGATC 1 cut(s) 58
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 2 cut(s) 118, 258
AcyI GRCGYC 1 cut(s) 5
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 1 cut(s) 181
AlwI GGATC 1 cut(s) 58
AlwNI CAGNNNCTG 1 cut(s) 81
Aor13HI TCCGGA 1 cut(s) 171
AoxI GGCC 1 cut(s) 189
ApoI RAATTY 2 cut(s) 118, 258
AspLEI GCGC 1 cut(s) 7
BalI TGGCCA 1 cut(s) 191
BanI GGYRCC 1 cut(s) 4
BccI CCATC 2 cut(s) 181, 302
BfaI CTAG 1 cut(s) 41
BfoI RGCGCY 1 cut(s) 8
BglII AGATCT 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 6
BmrI ACTGGG 1 cut(s) 138
BmsI GCATC 2 cut(s) 71, 238
BmuI ACTGGG 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 57
BsaWI WCCGGW 1 cut(s) 171
Bse1I ACTGG 2 cut(s) 75, 133
Bse3DI GCAATG 1 cut(s) 310
Bse8I GATNNNNATC 1 cut(s) 252
BseAI TCCGGA 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 1 cut(s) 279
BseJI GATNNNNATC 1 cut(s) 252
BseMI GCAATG 1 cut(s) 310
BseNI ACTGG 2 cut(s) 75, 133
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 1 cut(s) 191
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 1 cut(s) 172
BslFI GGGAC 1 cut(s) 259
BsmFI GGGAC 1 cut(s) 259
BsmI GAATGC 2 cut(s) 161, 233
BsnI GGCC 1 cut(s) 191
Bsp13I TCCGGA 1 cut(s) 171
Bsp143I GATC 2 cut(s) 50, 286
BspANI GGCC 1 cut(s) 191
BspEI TCCGGA 1 cut(s) 171
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 58
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 310
BsrI ACTGG 2 cut(s) 75, 133
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 2 cut(s) 50, 286
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 57
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 342
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 279
BstFNI CGCG 1 cut(s) 71
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 53, 289
BstMBI GATC 2 cut(s) 50, 286
BstMWI GCNNNNNNNGC 4 cut(s) 26, 68, 237, 342
BstUI CGCG 1 cut(s) 71
BstX2I RGATCY 2 cut(s) 50, 286
BstYI RGATCY 2 cut(s) 50, 286
BsuRI GGCC 1 cut(s) 191
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 69
CaiI CAGNNNCTG 1 cut(s) 81
CfoI GCGC 1 cut(s) 7
CseI GACGC 2 cut(s) 60, 261
CviAII CATG 2 cut(s) 31, 337
CviJI RGCY 5 cut(s) 20, 29, 191, 206, 245
CviKI_1 RGCY 5 cut(s) 20, 29, 191, 206, 245
DdeI CTNAG 1 cut(s) 314
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 52, 288
DpnII GATC 2 cut(s) 50, 286
EaeI YGGCCR 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 57
EcoRI GAATTC 1 cut(s) 258
EcoT14I CCWWGG 1 cut(s) 57
EcoT22I ATGCAT 1 cut(s) 233
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 2 cut(s) 34, 340
FaiI YATR 5 cut(s) 24, 32, 99, 138, 338
FaqI GGGAC 1 cut(s) 259
FatI CATG 2 cut(s) 30, 336
FokI GGATG 1 cut(s) 266
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 6
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 191
HapII CCGG 1 cut(s) 172
HgaI GACGC 2 cut(s) 60, 261
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 34, 340
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HpaII CCGG 1 cut(s) 172
Hpy188III TCNNGA 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 3 cut(s) 231, 303, 345
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 68, 237, 342
HpyF3I CTNAG 1 cut(s) 314
HpySE526I ACGT 1 cut(s) 176
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 34, 340
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 171
Kzo9I GATC 2 cut(s) 50, 286
LpnPI CCDG 4 cut(s) 88, 114, 128, 185
LweI GCATC 2 cut(s) 71, 238
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 161
MalI GATC 2 cut(s) 52, 288
MboI GATC 2 cut(s) 50, 286
MboII GAAGA 1 cut(s) 281
MfeI CAATTG 1 cut(s) 109
MflI RGATCY 2 cut(s) 50, 286
MlsI TGGCCA 1 cut(s) 191
MluCI AATT 3 cut(s) 109, 118, 258
MluI ACGCGT 1 cut(s) 69
MluNI TGGCCA 1 cut(s) 191
Mly113I GGCGCC 1 cut(s) 5
MnlI CCTC 5 cut(s) 18, 196, 222, 229, 287
Mox20I TGGCCA 1 cut(s) 191
Mph1103I ATGCAT 1 cut(s) 233
MroI TCCGGA 1 cut(s) 171
MscI TGGCCA 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 191
MspI CCGG 1 cut(s) 172
MunI CAATTG 1 cut(s) 109
Mva1269I GAATGC 2 cut(s) 161, 233
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 4 cut(s) 26, 68, 237, 342
NarI GGCGCC 1 cut(s) 5
NdeII GATC 2 cut(s) 50, 286
NlaIII CATG 2 cut(s) 34, 340
NlaIV GGNNCC 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 233
PcsI WCGNNNNNNNCGW 1 cut(s) 182
PctI GAATGC 2 cut(s) 161, 233
PluTI GGCGCC 1 cut(s) 8
PspN4I GGNNCC 1 cut(s) 6
PstNI CAGNNNCTG 1 cut(s) 81
PsuI RGATCY 2 cut(s) 50, 286
Sau3AI GATC 2 cut(s) 50, 286
SetI ASST 2 cut(s) 179, 214
SfaNI GCATC 2 cut(s) 71, 238
SfoI GGCGCC 1 cut(s) 6
Sse9I AATT 3 cut(s) 109, 118, 258
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 41
StyI CCWWGG 1 cut(s) 57
TaiI ACGT 1 cut(s) 179
TaqI TCGA 2 cut(s) 153, 256
TasI AATT 3 cut(s) 109, 118, 258
TspGWI ACGGA 1 cut(s) 157
XapI RAATTY 2 cut(s) 118, 258
XspI CTAG 1 cut(s) 41
Zsp2I ATGCAT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.