RLG00000018952

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005446
Physical Location & Seq
Forward (+)
43379412 .. 43381933
2522 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018952

Sequence Viewer

Length: 495 bp
ATGGCTGCTCGTCCTTTTTGTTATGTCTGTGTAACAAGTCTTCTTGTGCTTGTTCCTGTGCTGCTGCTTTGTTCATCATTAGGGAAGGCTCAGACGACGGATTACTGCACGAATGTGTTCGAGGAATTCAGCTCTTGCCTGAGTTTTGTCGGGGAGTTATCTCCTGAACCGACGGCGGCTTGCTGTGATTCCCTGACAAGTTTGAATTCGTTAGCACAACAAGAGGAGAGTGGTCCGAGGATGATATGTGAATGCATTGAGAGCTCGTCTTATTGGACCAGAATCCCTTTCAGTGCCTCCCGAATTCAGGAGCTCCCTACATCATGCCAACTCCATCTTAGTTTCCCCATTTCTAATAGCATGGATTGCTCCAAGTTCTATACTCCATCCCCCAAGTTCCCTGATGCCATGTCTGTAGAGGATTGTGTTCATCCCATGTTTCTCAATTATGGCTTCTCTGCCATCAGTGGTTGTCAGTGGCAATATCAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

165

Amino Acids

18.1

Weight (kDa)

4.51

Isoelectric Point (pI)

58.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 36 - 123 2e-06 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 176
AcsI RAATTY 3 cut(s) 125, 205, 303
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 1 cut(s) 205
AleI CACNNNNGTG 1 cut(s) 113
AluBI AGCT 4 cut(s) 132, 264, 313, 491
AluI AGCT 4 cut(s) 132, 264, 313, 491
Alw21I GWGCWC 2 cut(s) 266, 315
ApeKI GCWGC 3 cut(s) 5, 61, 64
ApoI RAATTY 3 cut(s) 125, 205, 303
Asp700I GAANNNNTTC 1 cut(s) 116
AspS9I GGNCC 2 cut(s) 233, 276
AvaII GGWCC 2 cut(s) 233, 276
BanII GRGCYC 2 cut(s) 266, 315
BarI GAAGNNNNNNTAC 2 cut(s) 24, 56
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 2 cut(s) 266, 315
BbvI GCAGC 2 cut(s) 48, 51
BccI CCATC 3 cut(s) 342, 394, 470
BceAI ACGGC 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 414
BisI GCNGC 4 cut(s) 6, 62, 65, 177
BlsI GCNGC 4 cut(s) 7, 63, 66, 178
Bme18I GGWCC 2 cut(s) 233, 276
BmgT120I GGNCC 2 cut(s) 233, 276
BmsI GCATC 1 cut(s) 394
BpiI GAAGAC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 236
Bsc4I CCNNNNNNNGG 1 cut(s) 307
BseDI CCNNGG 1 cut(s) 236
BseGI GGATG 3 cut(s) 246, 386, 430
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 2 cut(s) 104, 131
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 2 cut(s) 48, 51
BsgI GTGCAG 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 266, 315
BslI CCNNNNNNNGG 1 cut(s) 307
BsmI GAATGC 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 266, 315
BspACI CCGC 1 cut(s) 176
BspCNI CTCAG 2 cut(s) 103, 132
BssECI CCNNGG 1 cut(s) 236
BstAPI GCANNNNNTGC 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 3 cut(s) 90, 140, 338
BstF5I GGATG 3 cut(s) 246, 386, 430
BstMWI GCNNNNNNNGC 2 cut(s) 261, 366
BstSFI CTRYAG 1 cut(s) 414
BstV1I GCAGC 2 cut(s) 48, 51
BstV2I GAAGAC 1 cut(s) 32
BtsCI GGATG 3 cut(s) 246, 386, 430
BtsIMutI CAGTG 3 cut(s) 298, 472, 482
Cac8I GCNNGC 1 cut(s) 181
Cfr13I GGNCC 2 cut(s) 233, 276
CviAII CATG 4 cut(s) 324, 361, 409, 436
CviJI RGCY 8 cut(s) 5, 89, 132, 179, 264, 313, 453, 491
CviKI_1 RGCY 8 cut(s) 5, 89, 132, 179, 264, 313, 453, 491
DdeI CTNAG 3 cut(s) 90, 140, 338
Ecl136II GAGCTC 2 cut(s) 264, 313
Eco24I GRGCYC 2 cut(s) 266, 315
Eco47I GGWCC 2 cut(s) 233, 276
Eco53kI GAGCTC 2 cut(s) 264, 313
EcoICRI GAGCTC 2 cut(s) 264, 313
EcoRI GAATTC 3 cut(s) 125, 205, 303
EcoT22I ATGCAT 1 cut(s) 257
EcoT38I GRGCYC 2 cut(s) 266, 315
FaeI CATG 4 cut(s) 327, 364, 412, 439
FaiI YATR 8 cut(s) 24, 247, 325, 362, 381, 410, 437, 450
FatI CATG 4 cut(s) 323, 360, 408, 435
Fnu4HI GCNGC 4 cut(s) 6, 62, 65, 177
FokI GGATG 3 cut(s) 253, 373, 417
FriOI GRGCYC 2 cut(s) 266, 315
Fsp4HI GCNGC 4 cut(s) 6, 62, 65, 177
GluI GCNGC 4 cut(s) 6, 62, 65, 177
Hin1II CATG 4 cut(s) 327, 364, 412, 439
HindIII AAGCTT 1 cut(s) 489
HinfI GANTC 2 cut(s) 188, 282
Hpy188I TCNGA 2 cut(s) 93, 237
Hpy188III TCNNGA 3 cut(s) 164, 300, 308
Hpy99I CGWCG 2 cut(s) 100, 175
HpyAV CCTTC 1 cut(s) 79
HpyCH4V TGCA 2 cut(s) 108, 255
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 366
HpyF3I CTNAG 3 cut(s) 90, 140, 338
Hsp92II CATG 4 cut(s) 327, 364, 412, 439
LmnI GCTCC 3 cut(s) 310, 318, 374
LpnPI CCDG 7 cut(s) 69, 152, 177, 206, 292, 293, 414
Lsp1109I GCAGC 2 cut(s) 48, 51
LweI GCATC 1 cut(s) 394
MaeIII GTNAC 1 cut(s) 31
MboII GAAGA 1 cut(s) 32
MhlI GDGCHC 2 cut(s) 266, 315
MluCI AATT 4 cut(s) 125, 205, 303, 445
MnlI CCTC 5 cut(s) 115, 217, 231, 307, 412
Mph1103I ATGCAT 1 cut(s) 257
MroXI GAANNNNTTC 1 cut(s) 116
MslI CAYNNNNRTG 1 cut(s) 113
Mva1269I GAATGC 1 cut(s) 257
MwoI GCNNNNNNNGC 2 cut(s) 261, 366
NlaIII CATG 4 cut(s) 327, 364, 412, 439
NsiI ATGCAT 1 cut(s) 257
OliI CACNNNNGTG 1 cut(s) 113
PctI GAATGC 1 cut(s) 257
PdmI GAANNNNTTC 1 cut(s) 116
PfeI GAWTC 2 cut(s) 188, 282
PkrI GCNGC 4 cut(s) 7, 63, 66, 178
Psp124BI GAGCTC 2 cut(s) 266, 315
PspPI GGNCC 2 cut(s) 233, 276
RseI CAYNNNNRTG 1 cut(s) 113
SacI GAGCTC 2 cut(s) 266, 315
SatI GCNGC 4 cut(s) 6, 62, 65, 177
Sau96I GGNCC 2 cut(s) 233, 276
SduI GDGCHC 2 cut(s) 266, 315
SetI ASST 4 cut(s) 134, 266, 315, 493
SfaNI GCATC 1 cut(s) 394
SfcI CTRYAG 1 cut(s) 414
SinI GGWCC 2 cut(s) 233, 276
SmiMI CAYNNNNRTG 1 cut(s) 113
Sse9I AATT 4 cut(s) 125, 205, 303, 445
SsiI CCGC 1 cut(s) 176
SstI GAGCTC 2 cut(s) 266, 315
TaqI TCGA 1 cut(s) 120
TasI AATT 4 cut(s) 125, 205, 303, 445
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 2 cut(s) 188, 282
TscAI CASTG 3 cut(s) 298, 472, 482
TseI GCWGC 3 cut(s) 5, 61, 64
TspDTI ATGAA 2 cut(s) 63, 419
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 3 cut(s) 298, 472, 482
VpaK11BI GGWCC 2 cut(s) 233, 276
XapI RAATTY 3 cut(s) 125, 205, 303
XmnI GAANNNNTTC 1 cut(s) 116
Zsp2I ATGCAT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.