Rh2BG334400

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005446
Physical Location & Seq
Forward (+)
44157050 .. 44158248
1199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG334400.1

Sequence Viewer

Length: 354 bp
ATGGGTCTCCTTTTTGCTTATGTGATGATTTTTGCACTAATTTGTGGATCGGCCATGGGTCAAGCGTCCAGTTTCTGTGCTCAAGCCTGGAACGAGTTCAGCAACTGCATGAGGTTCATCACCGGGTTATTTTCGCAACCGTCAACGCAGTGCTGTGATTCGGTGAGGAGATTGAACAAGATGGCCAAAAGTAGTGAGAACGGACCTCGCGATATATGTCAATGCATTGAGGACATGTCTCGTACATATGGTATTCCTTTTGTAGCCTCCATCATCCAAGATCTACCCATCATATGCAATGCGCACCTTAGTTTCCCAATCTCTAATCGCATGAACTGCACCATGCTCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

117

Amino Acids

12.97

Weight (kDa)

7.49

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 26 - 113 4.3e-06 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 303
AccII CGCG 1 cut(s) 210
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 2 cut(s) 51, 183
AfaI GTAC 1 cut(s) 244
AflIII ACRYGT 1 cut(s) 234
AgsI TTSAA 1 cut(s) 175
AjnI CCWGG 1 cut(s) 86
AluBI AGCT 1 cut(s) 351
AluI AGCT 1 cut(s) 351
Alw21I GWGCWC 1 cut(s) 82
Alw26I GTCTC 2 cut(s) 11, 243
AlwI GGATC 1 cut(s) 55
AlwNI CAGNNNCTG 2 cut(s) 75, 105
AoxI GGCC 2 cut(s) 51, 183
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 304
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 112, 175
AvaII GGWCC 1 cut(s) 203
BalI TGGCCA 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 82
BccI CCATC 3 cut(s) 175, 278, 296
BciT130I CCWGG 1 cut(s) 88
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 2 cut(s) 11, 243
BglII AGATCT 1 cut(s) 280
BlpI GCTNAGC 1 cut(s) 347
Bme1390I CCNGG 2 cut(s) 88, 124
Bme18I GGWCC 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 203
BmrFI CCNGG 2 cut(s) 88, 124
Bpu1102I GCTNAGC 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 66
BpuMI CCSGG 1 cut(s) 124
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 54
Bse1I ACTGG 1 cut(s) 69
Bse3DI GCAATG 1 cut(s) 304
BseBI CCWGG 1 cut(s) 88
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 1 cut(s) 273
BseMI GCAATG 1 cut(s) 304
BseNI ACTGG 1 cut(s) 69
BseRI GAGGAG 1 cut(s) 181
BsgI GTGCAG 1 cut(s) 322
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 53, 185
BsiHKAI GWGCWC 1 cut(s) 82
BsiSI CCGG 1 cut(s) 123
BsmAI GTCTC 2 cut(s) 11, 243
BsnI GGCC 2 cut(s) 53, 185
Bso31I GGTCTC 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 2 cut(s) 47, 280
Bsp1720I GCTNAGC 1 cut(s) 347
Bsp19I CCATGG 1 cut(s) 54
Bsp68I TCGCGA 1 cut(s) 210
BspANI GGCC 2 cut(s) 53, 185
BspFNI CGCG 1 cut(s) 210
BspPI GGATC 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 304
BsrI ACTGG 1 cut(s) 69
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 2 cut(s) 47, 280
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 88
Bst4CI ACNGT 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 336
BstDEI CTNAG 2 cut(s) 308, 347
BstDSI CCRYGG 1 cut(s) 54
BstF5I GGATG 1 cut(s) 273
BstFNI CGCG 1 cut(s) 210
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 2 cut(s) 50, 283
BstMAI GTCTC 2 cut(s) 11, 243
BstMBI GATC 2 cut(s) 47, 280
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstNI CCWGG 1 cut(s) 88
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 2 cut(s) 86, 122
BstUI CGCG 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BsuRI GGCC 2 cut(s) 53, 185
BtgI CCRYGG 1 cut(s) 54
BtsCI GGATG 1 cut(s) 273
BtsI GCAGTG 1 cut(s) 155
BtsIMutI CAGTG 1 cut(s) 155
BtuMI TCGCGA 1 cut(s) 210
CaiI CAGNNNCTG 2 cut(s) 75, 105
CfoI GCGC 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 203
CseI GACGC 1 cut(s) 54
Csp6I GTAC 1 cut(s) 243
CviAII CATG 5 cut(s) 55, 109, 235, 331, 343
CviJI RGCY 5 cut(s) 53, 86, 185, 266, 351
CviKI_1 RGCY 5 cut(s) 53, 86, 185, 266, 351
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 2 cut(s) 308, 347
DpnI GATC 2 cut(s) 49, 282
DpnII GATC 2 cut(s) 47, 280
EaeI YGGCCR 2 cut(s) 51, 183
Eco130I CCWWGG 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 227
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 5 cut(s) 58, 112, 238, 334, 346
FatI CATG 5 cut(s) 54, 108, 234, 330, 342
FauNDI CATATG 2 cut(s) 247, 293
FokI GGATG 1 cut(s) 260
FspAI RTGCGCAY 1 cut(s) 303
FspI TGCGCA 1 cut(s) 303
GlaI GCGC 1 cut(s) 303
HaeIII GGCC 2 cut(s) 53, 185
HapII CCGG 1 cut(s) 123
HgaI GACGC 1 cut(s) 54
HhaI GCGC 1 cut(s) 304
Hin1II CATG 5 cut(s) 58, 112, 238, 334, 346
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HincII GTYRAC 1 cut(s) 144
HindII GTYRAC 1 cut(s) 144
HinfI GANTC 1 cut(s) 158
HpaII CCGG 1 cut(s) 123
HphI GGTGA 2 cut(s) 112, 175
Hpy166II GTNNAC 1 cut(s) 144
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 1 cut(s) 144
HpyCH4III ACNGT 1 cut(s) 141
HpyCH4V TGCA 5 cut(s) 35, 108, 225, 297, 339
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 2 cut(s) 308, 347
Hsp92II CATG 5 cut(s) 58, 112, 238, 334, 346
HspAI GCGC 1 cut(s) 302
Kzo9I GATC 2 cut(s) 47, 280
LpnPI CCDG 4 cut(s) 73, 82, 100, 136
MalI GATC 2 cut(s) 49, 282
MboI GATC 2 cut(s) 47, 280
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 82
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 1 cut(s) 39
MluNI TGGCCA 1 cut(s) 185
MnlI CCTC 5 cut(s) 105, 159, 216, 223, 277
Mox20I TGGCCA 1 cut(s) 185
Mph1103I ATGCAT 1 cut(s) 227
MroXI GAANNNNTTC 1 cut(s) 95
MscI TGGCCA 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 351
MspI CCGG 1 cut(s) 123
MspR9I CCNGG 2 cut(s) 88, 124
MvaI CCWGG 1 cut(s) 88
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 336
NciI CCSGG 1 cut(s) 124
NcoI CCATGG 1 cut(s) 54
NdeI CATATG 2 cut(s) 247, 293
NdeII GATC 2 cut(s) 47, 280
NlaIII CATG 5 cut(s) 58, 112, 238, 334, 346
NruI TCGCGA 1 cut(s) 210
NsbI TGCGCA 1 cut(s) 303
NsiI ATGCAT 1 cut(s) 227
NspI RCATGY 1 cut(s) 238
PciI ACATGT 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 1 cut(s) 158
PscI ACATGT 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 86
PspGI CCWGG 1 cut(s) 86
PspPI GGNCC 1 cut(s) 203
PstNI CAGNNNCTG 2 cut(s) 75, 105
PsuI RGATCY 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 351
RruI TCGCGA 1 cut(s) 210
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
Sau3AI GATC 2 cut(s) 47, 280
Sau96I GGNCC 1 cut(s) 203
ScrFI CCNGG 2 cut(s) 88, 124
SduI GDGCHC 1 cut(s) 82
SetI ASST 4 cut(s) 116, 208, 309, 353
SinI GGWCC 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
Sse9I AATT 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 86, 122
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 1 cut(s) 141
TasI AATT 1 cut(s) 39
TfiI GAWTC 1 cut(s) 158
TscAI CASTG 1 cut(s) 155
TspDTI ATGAA 2 cut(s) 106, 347
TspGWI ACGGA 1 cut(s) 216
TspRI CASTG 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 203
XceI RCATGY 1 cut(s) 238
XmnI GAANNNNTTC 1 cut(s) 95
Zsp2I ATGCAT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.