Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0003064

Physical Location & Seq

Genomic Coordinates Reverse (-)

26866775 .. 26869286

Gene Structure 2512 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1126900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1098 bp

ATGGAAGGATGCACACACTACTTGGCAATGGTACTGGTTCAGCTGATTTACGGCGGATCAGACGTCCTCATAAAATTATCCCTTCAGGATGGACTCAATCCGATTGTCTTTGTGGTTTACAGGCATGTTCTTGCCATGGTTTTGATAGGACCATTTGCTTATGTGCTTGAAAGGAAGCAGAGGCCTTCGTTCTCAATTTCAGCAGCTGCAAATATTTTCCTGCTAGCGTTGTTAGGGCCTACCATCTTTGTGAATGTATACTGTGCTGGTTTGACATACACTTCTGCAACAGTTGCAAGTGCCTTAAGTAATGTTATCCCCCCGTTGACGTTTCTGACGGCAGTTCTTCTCGGGATGGAGAAACTAAAGATTAGAAGCGCTCGAGGCCAAGCAAAGGTGGCTGGTACAATTTTCTGCATCGGCGGATCTCTTATTTTCACATTCTGGAAAGGAGGATACCTGTTGAAGGGTGTCGAAAAGACACTGATAAATGTTCACAACGCAGAGGAGTATGGCAAGATCAAGCATGTTCAGGAAAACTGGATCAAGGGTTCTGCTCTTCTTCTGATGAGCTACACTGCGTGGAGTGCGTGGCTAATCCTCCAGGCTGCGGTGTCCAAAGTCTACCCTGCGCCATTGTCACTAACCACCCTAATGTGCTTTTTCGCTTCGCTGCAATCTTCTTTCCTCGCCCTGTTTTTTGCTAGAAATCCAAGCTCATGGAGACTGGAGTGGAACCTGCAGCTTTTAACCATCGTCTACTTCGGAGTTCTGACCACAGCATTAGTCTATTACCTGCAAGCATGGTGCATCAGTCACAAGGGACCAGTTTTTGCAGCTATGTTTAGTCCACTTCAAGCCATTATAGTGGCAGTGTTTTCGGCAATTGCTTTTGCAGAGCGACTTCACTTTGGCAGCTTGATTGGAGCACTTCTCATTATTGTGGGACTTTACTGTGTGCTGTGGGGAAAGAGGAAAGACACTCTTGTTGCTGAACATGGAGAGATTGAAAAAGTGACACTGGACGATATTAAAGTGCTGGAGATTTCCATGAATGATATTTCAGTAGTCAATCCTGTTACTAGAGAAGAAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005938 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009987 GO:0010033 GO:0016020 GO:0016021 GO:0023052 GO:0031224 GO:0031226 GO:0032870 GO:0042221 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

366

Amino Acids

40.18

Weight (kDa)

8.91

Isoelectric Point (pI)

29.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	7 - 145	9.7e-14	EamA-like transporter family
EamA	PF00892	183 - 321	6e-14	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003064)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g10210 FvH4_5g10220
malus_domestica	MD06G1126900.v1.1 MD06G1127200.v1.1 MD14G1142400.v1.1
prunus_persica	Prupe.4G100300_v2.0.a1 Prupe.5G136500_v2.0.a1 Prupe.5G136500_v2.0.a1
pyrus_communis	pycom06g11660 pycom14g11780 pycom14g11810
rosa_chinensis	RchiOBHm_Chr7g0188001 RchiOBHm_Chr7g0188011
rosa_laevigata	RLG00000004733 RLG00000004734 RLG00000004735
rosa_multiflora	Rmu_co8503989.1_g000001 Rmu_sc0003624.1_g000020 Rmu_ssc0000422.1_g000012
rosa_roxburghii	Rroxscaffold_3G00266810 Rroxscaffold_3G00266820
rosa_rugosa	Rorug06G0486700 Rorug06G0486700 Rorug06G0486800 Rorug06G0486900
rosa_samantha	Rh7AG091600 Rh7AG091700 Rh7BG093100 Rh7BG093200 Rh7BG093300 Rh7CG092400 Rh7CG092500 Rh7CG092700 Rh7DG093800 Rh7DG093900
rosa_wichuraiana	Rw7G007900 Rw7G007910 Rw7G007920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	66
Acc36I	ACCTGC	2 cut(s)	747, 804
AccI	GTMKAC	3 cut(s)	258, 624, 759
AciI	CCGC	3 cut(s)	54, 423, 611
AclWI	GGATC	3 cut(s)	64, 433, 551
AcuI	CTGAAG	1 cut(s)	68
AcyI	GRCGYC	1 cut(s)	63
AdeI	CACNNNGTG	1 cut(s)	582
AfaI	GTAC	2 cut(s)	33, 406
AfeI	AGCGCT	1 cut(s)	379
AfiI	CCNNNNNNNGG	3 cut(s)	394, 466, 610
AflII	CTTAAG	1 cut(s)	304
AgsI	TTSAA	4 cut(s)	170, 466, 857, 1010
AjnI	CCWGG	1 cut(s)	603
AloI	GAACNNNNNNTCC	2 cut(s)	535, 567
AluBI	AGCT	7 cut(s)	43, 206, 573, 717, 745, 839, 918
AluI	AGCT	7 cut(s)	43, 206, 573, 717, 745, 839, 918
Alw21I	GWGCWC	1 cut(s)	931
Alw26I	GTCTC	1 cut(s)	718
AlwI	GGATC	3 cut(s)	64, 433, 551
AlwNI	CAGNNNCTG	1 cut(s)	206
Ama87I	CYCGRG	2 cut(s)	350, 381
Aor51HI	AGCGCT	1 cut(s)	379
AoxI	GGCC	3 cut(s)	182, 236, 385
ApeKI	GCWGC	7 cut(s)	203, 206, 608, 673, 742, 836, 915
AspLEI	GCGC	2 cut(s)	380, 634
AspS9I	GGNCC	3 cut(s)	149, 236, 824
AsuNHI	GCTAGC	1 cut(s)	223
AvaI	CYCGRG	2 cut(s)	350, 381
AvaII	GGWCC	2 cut(s)	149, 824
Bbv12I	GWGCWC	1 cut(s)	931
BbvI	GCAGC	7 cut(s)	193, 215, 595, 660, 754, 848, 927
BccI	CCATC	4 cut(s)	83, 251, 349, 761
BceAI	ACGGC	2 cut(s)	67, 354
BcgI	CGANNNNNNTGC	2 cut(s)	861, 895
BciT130I	CCWGG	1 cut(s)	605
BciVI	GTATCC	1 cut(s)	449
BcoDI	GTCTC	1 cut(s)	718
BfaI	CTAG	3 cut(s)	224, 705, 1083
BfmI	CTRYAG	1 cut(s)	740
BfoI	RGCGCY	1 cut(s)	381
BfrI	CTTAAG	1 cut(s)	304
BfuAI	ACCTGC	2 cut(s)	747, 804
BfuI	GTATCC	1 cut(s)	449
BisI	GCNGC	7 cut(s)	204, 207, 609, 674, 743, 837, 916
BlsI	GCNGC	7 cut(s)	205, 208, 610, 675, 744, 838, 917
Bme1390I	CCNGG	1 cut(s)	605
Bme18I	GGWCC	2 cut(s)	149, 824
BmeT110I	CYCGRG	2 cut(s)	350, 381
BmgT120I	GGNCC	3 cut(s)	149, 236, 824
BmiI	GGNNCC	2 cut(s)	737, 825
BmrFI	CCNGG	1 cut(s)	605
BmsI	GCATC	2 cut(s)	426, 819
BmtI	GCTAGC	1 cut(s)	227
BplI	GAGNNNNNCTC	2 cut(s)	918, 950
BpmI	CTGGAG	3 cut(s)	587, 749, 1061
BsaHI	GRCGYC	1 cut(s)	63
BsaJI	CCNNGG	1 cut(s)	135
Bsc4I	CCNNNNNNNGG	3 cut(s)	394, 466, 610
Bse1I	ACTGG	5 cut(s)	39, 545, 732, 827, 1026
Bse3DI	GCAATG	1 cut(s)	33
BseBI	CCWGG	1 cut(s)	605
BseDI	CCNNGG	1 cut(s)	135
BseGI	GGATG	3 cut(s)	14, 94, 360
BseLI	CCNNNNNNNGG	3 cut(s)	394, 466, 610
BseMI	GCAATG	1 cut(s)	33
BseNI	ACTGG	5 cut(s)	39, 545, 732, 827, 1026
BseRI	GAGGAG	1 cut(s)	521
BseXI	GCAGC	7 cut(s)	193, 215, 595, 660, 754, 848, 927
BshFI	GGCC	3 cut(s)	184, 238, 387
BsiHKAI	GWGCWC	1 cut(s)	931
BsiHKCI	CYCGRG	2 cut(s)	350, 381
BslFI	GGGAC	2 cut(s)	837, 960
BslI	CCNNNNNNNGG	3 cut(s)	394, 466, 610
BsmAI	GTCTC	1 cut(s)	718
BsmFI	GGGAC	2 cut(s)	837, 960
BsnI	GGCC	3 cut(s)	184, 238, 387
BsoBI	CYCGRG	2 cut(s)	350, 381
Bsp1286I	GDGCHC	1 cut(s)	931
Bsp143I	GATC	4 cut(s)	56, 425, 519, 543
Bsp19I	CCATGG	1 cut(s)	135
BspACI	CCGC	3 cut(s)	54, 423, 611
BspANI	GGCC	3 cut(s)	184, 238, 387
BspLI	GGNNCC	2 cut(s)	737, 825
BspMAI	CTGCAG	1 cut(s)	744
BspMI	ACCTGC	2 cut(s)	747, 804
BspOI	GCTAGC	1 cut(s)	227
BspPI	GGATC	3 cut(s)	64, 433, 551
BspQI	GCTCTTC	1 cut(s)	564
BspTI	CTTAAG	1 cut(s)	304
BsrDI	GCAATG	1 cut(s)	33
BsrI	ACTGG	5 cut(s)	39, 545, 732, 827, 1026
BssECI	CCNNGG	1 cut(s)	135
BssMI	GATC	4 cut(s)	56, 425, 519, 543
BssNAI	GTATAC	1 cut(s)	259
BssNI	GRCGYC	1 cut(s)	63
BssT1I	CCWWGG	1 cut(s)	135
Bst1107I	GTATAC	1 cut(s)	259
Bst2UI	CCWGG	1 cut(s)	605
Bst4CI	ACNGT	3 cut(s)	263, 292, 956
Bst6I	CTCTTC	1 cut(s)	564
BstACI	GRCGYC	1 cut(s)	63
BstAFI	CTTAAG	1 cut(s)	304
BstAPI	GCANNNNNTGC	1 cut(s)	293
BstC8I	GCNNGC	2 cut(s)	225, 801
BstDSI	CCRYGG	1 cut(s)	135
BstENI	CCTNNNNNAGG	1 cut(s)	464
BstF5I	GGATG	3 cut(s)	14, 94, 360
BstH2I	RGCGCY	1 cut(s)	381
BstHHI	GCGC	2 cut(s)	380, 634
BstKTI	GATC	4 cut(s)	59, 428, 522, 546
BstMAI	GTCTC	1 cut(s)	718
BstMBI	GATC	4 cut(s)	56, 425, 519, 543
BstMWI	GCNNNNNNNGC	4 cut(s)	293, 384, 398, 587
BstNI	CCWGG	1 cut(s)	605
BstNSI	RCATGY	2 cut(s)	128, 530
BstSCI	CCNGG	1 cut(s)	603
BstSFI	CTRYAG	1 cut(s)	740
BstV1I	GCAGC	7 cut(s)	193, 215, 595, 660, 754, 848, 927
BstX2I	RGATCY	1 cut(s)	425
BstXI	CCANNNNNNTGG	2 cut(s)	720, 868
BstYI	RGATCY	1 cut(s)	425
BstZ17I	GTATAC	1 cut(s)	259
BsuI	GTATCC	1 cut(s)	449
BsuRI	GGCC	3 cut(s)	184, 238, 387
BtgI	CCRYGG	1 cut(s)	135
BtsCI	GGATG	3 cut(s)	14, 94, 360
BtsI	GCAGTG	2 cut(s)	576, 879
BtsIMutI	CAGTG	4 cut(s)	482, 576, 879, 1019
BveI	ACCTGC	2 cut(s)	747, 804
Cac8I	GCNNGC	2 cut(s)	225, 801
CaiI	CAGNNNCTG	1 cut(s)	206
CfoI	GCGC	2 cut(s)	380, 634
Cfr13I	GGNCC	3 cut(s)	149, 236, 824
Csp6I	GTAC	2 cut(s)	32, 405
CviAII	CATG	8 cut(s)	125, 136, 527, 720, 804, 998, 1051, 1095
CviQI	GTAC	2 cut(s)	32, 405
DpnI	GATC	4 cut(s)	58, 427, 521, 545
DpnII	GATC	4 cut(s)	56, 425, 519, 543
DraIII	CACNNNGTG	1 cut(s)	582
Eam1104I	CTCTTC	1 cut(s)	564
EarI	CTCTTC	1 cut(s)	564
EciI	GGCGGA	2 cut(s)	69, 438
Eco130I	CCWWGG	1 cut(s)	135
Eco147I	AGGCCT	1 cut(s)	184
Eco47I	GGWCC	2 cut(s)	149, 824
Eco47III	AGCGCT	1 cut(s)	379
Eco57I	CTGAAG	1 cut(s)	68
Eco88I	CYCGRG	2 cut(s)	350, 381
EcoNI	CCTNNNNNAGG	1 cut(s)	464
EcoO109I	RGGNCCY	1 cut(s)	236
EcoRII	CCWGG	1 cut(s)	603
EcoT14I	CCWWGG	1 cut(s)	135
ErhI	CCWWGG	1 cut(s)	135
FaeI	CATG	8 cut(s)	128, 139, 530, 723, 807, 1001, 1054, 1098
FalI	AAGNNNNNCTT	2 cut(s)	969, 1001
FaqI	GGGAC	2 cut(s)	837, 960
FatI	CATG	8 cut(s)	124, 135, 526, 719, 803, 997, 1050, 1094
FblI	GTMKAC	3 cut(s)	258, 624, 759
Fnu4HI	GCNGC	7 cut(s)	204, 207, 609, 674, 743, 837, 916
FokI	GGATG	3 cut(s)	21, 101, 367
Fsp4HI	GCNGC	7 cut(s)	204, 207, 609, 674, 743, 837, 916
FspBI	CTAG	3 cut(s)	224, 705, 1083
GlaI	GCGC	2 cut(s)	379, 633
GluI	GCNGC	7 cut(s)	204, 207, 609, 674, 743, 837, 916
GsuI	CTGGAG	3 cut(s)	587, 749, 1061
HaeII	RGCGCY	1 cut(s)	381
HaeIII	GGCC	3 cut(s)	184, 238, 387
HhaI	GCGC	2 cut(s)	380, 634
Hin1I	GRCGYC	1 cut(s)	63
Hin1II	CATG	8 cut(s)	128, 139, 530, 723, 807, 1001, 1054, 1098
Hin6I	GCGC	2 cut(s)	378, 632
HinP1I	GCGC	2 cut(s)	378, 632
HincII	GTYRAC	1 cut(s)	327
HindII	GTYRAC	1 cut(s)	327
HinfI	GANTC	1 cut(s)	93
Hpy166II	GTNNAC	7 cut(s)	118, 259, 327, 496, 625, 760, 851
Hpy188I	TCNGA	6 cut(s)	61, 102, 336, 567, 767, 774
Hpy188III	TCNNGA	4 cut(s)	86, 352, 445, 533
Hpy8I	GTNNAC	7 cut(s)	118, 259, 327, 496, 625, 760, 851
HpyAV	CCTTC	3 cut(s)	92, 195, 460
HpyCH4III	ACNGT	3 cut(s)	263, 292, 956
HpyCH4IV	ACGT	2 cut(s)	63, 329
HpyF10VI	GCNNNNNNNGC	4 cut(s)	293, 384, 398, 587
HpySE526I	ACGT	2 cut(s)	63, 329
Hsp92I	GRCGYC	1 cut(s)	63
Hsp92II	CATG	8 cut(s)	128, 139, 530, 723, 807, 1001, 1054, 1098
HspAI	GCGC	2 cut(s)	378, 632
Kzo9I	GATC	4 cut(s)	56, 425, 519, 543
LguI	GCTCTTC	1 cut(s)	564
LmnI	GCTCC	1 cut(s)	926
Lsp1109I	GCAGC	7 cut(s)	193, 215, 595, 660, 754, 848, 927
LweI	GCATC	2 cut(s)	426, 819
MaeI	CTAG	3 cut(s)	224, 705, 1083
MaeII	ACGT	2 cut(s)	63, 329
MaeIII	GTNAC	4 cut(s)	639, 815, 1015, 1078
MalI	GATC	4 cut(s)	58, 427, 521, 545
MboI	GATC	4 cut(s)	56, 425, 519, 543
MboII	GAAGA	4 cut(s)	338, 551, 554, 672
MfeI	CAATTG	1 cut(s)	885
MflI	RGATCY	1 cut(s)	425
MhlI	GDGCHC	1 cut(s)	931
MluCI	AATT	4 cut(s)	74, 195, 408, 885
MlyI	GAGTC	1 cut(s)	87
MnlI	CCTC	8 cut(s)	77, 174, 377, 446, 499, 611, 698, 966
MseI	TTAA	3 cut(s)	305, 749, 1032
MslI	CAYNNNNRTG	4 cut(s)	248, 653, 866, 941
MspA1I	CMGCKG	2 cut(s)	43, 206
MspCI	CTTAAG	1 cut(s)	304
MspR9I	CCNGG	1 cut(s)	605
MunI	CAATTG	1 cut(s)	885
MvaI	CCWGG	1 cut(s)	605
MwoI	GCNNNNNNNGC	4 cut(s)	293, 384, 398, 587
NcoI	CCATGG	1 cut(s)	135
NdeII	GATC	4 cut(s)	56, 425, 519, 543
NheI	GCTAGC	1 cut(s)	223
NlaIII	CATG	8 cut(s)	128, 139, 530, 723, 807, 1001, 1054, 1098
NlaIV	GGNNCC	2 cut(s)	737, 825
NmuCI	GTSAC	3 cut(s)	639, 815, 1015
NspI	RCATGY	2 cut(s)	128, 530
PaeR7I	CTCGAG	1 cut(s)	381
PceI	AGGCCT	1 cut(s)	184
PciSI	GCTCTTC	1 cut(s)	564
PkrI	GCNGC	7 cut(s)	205, 208, 610, 675, 744, 838, 917
PleI	GAGTC	1 cut(s)	87
PpsI	GAGTC	1 cut(s)	87
Psp6I	CCWGG	1 cut(s)	603
PspGI	CCWGG	1 cut(s)	603
PspN4I	GGNNCC	2 cut(s)	737, 825
PspPI	GGNCC	3 cut(s)	149, 236, 824
PspXI	VCTCGAGB	1 cut(s)	381
PstI	CTGCAG	1 cut(s)	744
PstNI	CAGNNNCTG	1 cut(s)	206
PsuI	RGATCY	1 cut(s)	425
PvuII	CAGCTG	2 cut(s)	43, 206
RsaI	GTAC	2 cut(s)	33, 406
RsaNI	GTAC	2 cut(s)	32, 405
RseI	CAYNNNNRTG	4 cut(s)	248, 653, 866, 941
SapI	GCTCTTC	1 cut(s)	564
SaqAI	TTAA	3 cut(s)	305, 749, 1032
SatI	GCNGC	7 cut(s)	204, 207, 609, 674, 743, 837, 916
Sau3AI	GATC	4 cut(s)	56, 425, 519, 543
Sau96I	GGNCC	3 cut(s)	149, 236, 824
SchI	GAGTC	1 cut(s)	87
ScrFI	CCNGG	1 cut(s)	605
SduI	GDGCHC	1 cut(s)	931
SfaNI	GCATC	2 cut(s)	426, 819
SfcI	CTRYAG	1 cut(s)	740
Sfr274I	CTCGAG	1 cut(s)	381
SinI	GGWCC	2 cut(s)	149, 824
SlaI	CTCGAG	1 cut(s)	381
SmiMI	CAYNNNNRTG	4 cut(s)	248, 653, 866, 941
SmlI	CTYRAG	2 cut(s)	304, 381
SmoI	CTYRAG	2 cut(s)	304, 381
Sse9I	AATT	4 cut(s)	74, 195, 408, 885
SseBI	AGGCCT	1 cut(s)	184
SsiI	CCGC	3 cut(s)	54, 423, 611
SspI	AATATT	1 cut(s)	214
SspMI	CTAG	3 cut(s)	224, 705, 1083
StuI	AGGCCT	1 cut(s)	184
StyD4I	CCNGG	1 cut(s)	603
StyI	CCWWGG	1 cut(s)	135
TaaI	ACNGT	3 cut(s)	263, 292, 956
TaiI	ACGT	2 cut(s)	66, 332
TaqI	TCGA	2 cut(s)	382, 474
TasI	AATT	4 cut(s)	74, 195, 408, 885
Tru1I	TTAA	3 cut(s)	305, 749, 1032
Tru9I	TTAA	3 cut(s)	305, 749, 1032
TscAI	CASTG	4 cut(s)	489, 583, 879, 1026
TseFI	GTSAC	3 cut(s)	639, 815, 1015
TseI	GCWGC	7 cut(s)	203, 206, 608, 673, 742, 836, 915
Tsp45I	GTSAC	3 cut(s)	639, 815, 1015
TspDTI	ATGAA	1 cut(s)	1067
TspRI	CASTG	4 cut(s)	489, 583, 879, 1026
Vha464I	CTTAAG	1 cut(s)	304
VpaK11BI	GGWCC	2 cut(s)	149, 824
XagI	CCTNNNNNAGG	1 cut(s)	464
XceI	RCATGY	2 cut(s)	128, 530
XhoI	CTCGAG	1 cut(s)	381
XmiI	GTMKAC	3 cut(s)	258, 624, 759
XspI	CTAG	3 cut(s)	224, 705, 1083
ZraI	GACGTC	1 cut(s)	64

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.