Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003064

Physical Location & Seq

Genomic Coordinates Reverse (-)

23507095 .. 23508915

Gene Structure 1821 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1142400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1110 bp

ATGGGAGGAAGCACTAATTATTACTCGGCAATGATACTGGTTCAGCTGATATACGGCGGATCAAACGTACTTATGAAACTATCCCTTCAGGAAGGACTCAATCAGATTGTCTTCGTGGTTTACAGGCATGTTCTTGCCATGGTTTTGGTAGGACCATTTGCTTATGTGCTTGAAAGGAAGCAGAGGCCTTCGCTCTCATTTTCGGTAGCTGCAAAGATTTTTGCTCTTGCATCACTCGGGACTACCATCCATCTTAATCTGTACTATGCTGGTTTAGCATACACTTCTGCAACGGTTGGATGTGCCTTGAGTAATGTTATCCCTGCTTTGACGTTTCTAGTGGCAGTTCTTCTCAGGATGGAGAAATTGAACATTAGAAGCACTCGAGGTCAAGCTAAGGTGGTCGGGACCCTTCTTTGCCTTGGCGGTTCTCTTATTTTCACCTTCTGGAAAGGAGGATACCTATATAAAGGTTTTTTTGAGAAGCCACTGATACATGGTTTCAACGCTGAGTCTGTAGGCAAGATTCGGCATGTCAAAGAAAACTGGATCAAGGGTTCTGTGCTGATTCTGACGAGTTACACTGCATGGAGTGCATGGCTAATCCTCCAGGCTGTGATCTCCAAAGTCTACCCTGCTCCATTGTCACTAACCACCATGGTATGCTTTTCTGCATCGCTGCAATCTTCTTTCCTTGCCCTGTTTTTCGCTAGGAATCCAAGCTCATGGAAACTGGAGTGGAACCTTGAGCTGTTAACCATCGTCTACTTTGGAGTTCTGACCACAGCATTAGTCTATTGCCTGCAAACATGGTGCATCAGTCACAAGGGACCAGTTTTTTCAGCAATGTTTAGTCCACTTCAAGCCGTTATAGTGGCAGTGTTTTCGGCAATTGCTTTCGCGGAGCGACTTCACTTTGGCAGCTTGATTGGAGCACTTCTCATTATTGCCGGCCTTTACAGCGTTCTGTGGGGAAAGAGGAAAGACAGTCTGGTTGCTGAACACGCAGAGATTGGAAAAATGACGATAGACGATAACAAAGTTGTAGAGAGTTCCATGAATGAAATTTCAGTAACCAATCCGGTCAGTGGAGAAAAGACGAGATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005938 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009987 GO:0010033 GO:0016020 GO:0016021 GO:0023052 GO:0031224 GO:0031226 GO:0032870 GO:0042221 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

370

Amino Acids

40.47

Weight (kDa)

9.5

Isoelectric Point (pI)

30.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	8 - 147	2.1e-13	EamA-like transporter family
EamA	PF00892	185 - 323	1.1e-12	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003064)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g10210 FvH4_5g10220
malus_domestica	MD06G1126900.v1.1 MD06G1127200.v1.1 MD14G1142400.v1.1
prunus_persica	Prupe.4G100300_v2.0.a1 Prupe.5G136500_v2.0.a1 Prupe.5G136500_v2.0.a1
pyrus_communis	pycom06g11660 pycom14g11780 pycom14g11810
rosa_chinensis	RchiOBHm_Chr7g0188001 RchiOBHm_Chr7g0188011
rosa_laevigata	RLG00000004733 RLG00000004734 RLG00000004735
rosa_multiflora	Rmu_co8503989.1_g000001 Rmu_sc0003624.1_g000020 Rmu_ssc0000422.1_g000012
rosa_roxburghii	Rroxscaffold_3G00266810 Rroxscaffold_3G00266820
rosa_rugosa	Rorug06G0486700 Rorug06G0486700 Rorug06G0486800 Rorug06G0486900
rosa_samantha	Rh7AG091600 Rh7AG091700 Rh7BG093100 Rh7BG093200 Rh7BG093300 Rh7CG092400 Rh7CG092500 Rh7CG092700 Rh7DG093800 Rh7DG093900
rosa_wichuraiana	Rw7G007900 Rw7G007910 Rw7G007920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	630, 765
AccII	CGCG	1 cut(s)	902
AciI	CCGC	3 cut(s)	57, 426, 902
AclWI	GGATC	2 cut(s)	67, 557
AcsI	RAATTY	1 cut(s)	1065
AcuI	CTGAAG	1 cut(s)	71
AfaI	GTAC	2 cut(s)	69, 263
AfiI	CCNNNNNNNGG	1 cut(s)	1088
AgsI	TTSAA	4 cut(s)	173, 370, 505, 863
AjnI	CCWGG	1 cut(s)	609
AloI	GAACNNNNNNTCC	2 cut(s)	541, 573
AluBI	AGCT	6 cut(s)	46, 209, 395, 723, 751, 924
AluI	AGCT	6 cut(s)	46, 209, 395, 723, 751, 924
Alw21I	GWGCWC	1 cut(s)	937
AlwI	GGATC	2 cut(s)	67, 557
Ama87I	CYCGRG	2 cut(s)	236, 384
AoxI	GGCC	2 cut(s)	185, 952
ApeKI	GCWGC	3 cut(s)	209, 679, 921
ApoI	RAATTY	1 cut(s)	1065
AspS9I	GGNCC	3 cut(s)	152, 408, 830
AsuHPI	GGTGA	1 cut(s)	433
AvaI	CYCGRG	2 cut(s)	236, 384
AvaII	GGWCC	3 cut(s)	152, 408, 830
BbsI	GAAGAC	1 cut(s)	103
Bbv12I	GWGCWC	1 cut(s)	937
BbvI	GCAGC	3 cut(s)	196, 666, 933
BccI	CCATC	4 cut(s)	254, 258, 352, 767
BceAI	ACGGC	2 cut(s)	70, 851
BcgI	CGANNNNNNTGC	2 cut(s)	867, 901
BciT130I	CCWGG	1 cut(s)	611
BciVI	GTATCC	1 cut(s)	452
BfaI	CTAG	2 cut(s)	338, 711
BfmI	CTRYAG	1 cut(s)	516
BfuI	GTATCC	1 cut(s)	452
BisI	GCNGC	3 cut(s)	210, 680, 922
BlsI	GCNGC	3 cut(s)	211, 681, 923
Bme1390I	CCNGG	1 cut(s)	611
Bme18I	GGWCC	3 cut(s)	152, 408, 830
BmeT110I	CYCGRG	2 cut(s)	236, 384
BmgT120I	GGNCC	3 cut(s)	152, 408, 830
BmiI	GGNNCC	4 cut(s)	409, 410, 743, 831
BmrFI	CCNGG	1 cut(s)	611
BmsI	GCATC	3 cut(s)	239, 683, 825
BpiI	GAAGAC	1 cut(s)	103
BplI	GAGNNNNNCTC	2 cut(s)	924, 956
BpmI	CTGGAG	2 cut(s)	593, 755
Bpu10I	CCTNAGC	1 cut(s)	396
BpuEI	CTTGAG	2 cut(s)	328, 767
BsaJI	CCNNGG	3 cut(s)	138, 421, 657
BsaWI	WCCGGW	1 cut(s)	1081
Bsc4I	CCNNNNNNNGG	1 cut(s)	1088
Bse118I	RCCGGY	1 cut(s)	950
Bse1I	ACTGG	4 cut(s)	42, 551, 738, 833
Bse3DI	GCAATG	2 cut(s)	36, 852
BseBI	CCWGG	1 cut(s)	611
BseDI	CCNNGG	3 cut(s)	138, 421, 657
BseGI	GGATG	3 cut(s)	246, 305, 363
BseLI	CCNNNNNNNGG	1 cut(s)	1088
BseMI	GCAATG	2 cut(s)	36, 852
BseMII	CTCAG	2 cut(s)	367, 501
BseNI	ACTGG	4 cut(s)	42, 551, 738, 833
BseXI	GCAGC	3 cut(s)	196, 666, 933
Bsh1236I	CGCG	1 cut(s)	902
BshFI	GGCC	2 cut(s)	187, 954
BsiHKAI	GWGCWC	1 cut(s)	937
BsiHKCI	CYCGRG	2 cut(s)	236, 384
BsiSI	CCGG	2 cut(s)	951, 1082
BslFI	GGGAC	3 cut(s)	253, 421, 843
BslI	CCNNNNNNNGG	1 cut(s)	1088
BsmFI	GGGAC	3 cut(s)	253, 421, 843
BsnI	GGCC	2 cut(s)	187, 954
BsoBI	CYCGRG	2 cut(s)	236, 384
Bsp1286I	GDGCHC	1 cut(s)	937
Bsp143I	GATC	4 cut(s)	59, 549, 618, 1103
Bsp19I	CCATGG	2 cut(s)	138, 657
BspACI	CCGC	3 cut(s)	57, 426, 902
BspANI	GGCC	2 cut(s)	187, 954
BspCNI	CTCAG	2 cut(s)	366, 502
BspFNI	CGCG	1 cut(s)	902
BspHI	TCATGA	1 cut(s)	1106
BspLI	GGNNCC	4 cut(s)	409, 410, 743, 831
BspPI	GGATC	2 cut(s)	67, 557
BsrDI	GCAATG	2 cut(s)	36, 852
BsrFI	RCCGGY	1 cut(s)	950
BsrI	ACTGG	4 cut(s)	42, 551, 738, 833
BssAI	RCCGGY	1 cut(s)	950
BssECI	CCNNGG	3 cut(s)	138, 421, 657
BssMI	GATC	4 cut(s)	59, 549, 618, 1103
BssT1I	CCWWGG	3 cut(s)	138, 421, 657
Bst2UI	CCWGG	1 cut(s)	611
Bst4CI	ACNGT	2 cut(s)	295, 989
BstAPI	GCANNNNNTGC	1 cut(s)	593
BstC8I	GCNNGC	2 cut(s)	803, 952
BstDEI	CTNAG	3 cut(s)	353, 396, 510
BstDSI	CCRYGG	2 cut(s)	138, 657
BstF5I	GGATG	3 cut(s)	246, 305, 363
BstFNI	CGCG	1 cut(s)	902
BstKTI	GATC	4 cut(s)	62, 552, 621, 1106
BstMBI	GATC	4 cut(s)	59, 549, 618, 1103
BstMWI	GCNNNNNNNGC	4 cut(s)	275, 593, 960, 1004
BstNI	CCWGG	1 cut(s)	611
BstNSI	RCATGY	2 cut(s)	131, 536
BstSCI	CCNGG	1 cut(s)	609
BstSFI	CTRYAG	1 cut(s)	516
BstUI	CGCG	1 cut(s)	902
BstV1I	GCAGC	3 cut(s)	196, 666, 933
BstV2I	GAAGAC	1 cut(s)	103
BstXI	CCANNNNNNTGG	2 cut(s)	145, 726
BsuI	GTATCC	1 cut(s)	452
BsuRI	GGCC	2 cut(s)	187, 954
BtgI	CCRYGG	2 cut(s)	138, 657
BtgZI	GCGATG	1 cut(s)	660
BtsCI	GGATG	3 cut(s)	246, 305, 363
BtsI	GCAGTG	2 cut(s)	582, 885
BtsIMutI	CAGTG	4 cut(s)	488, 582, 885, 1093
Cac8I	GCNNGC	2 cut(s)	803, 952
CciI	TCATGA	1 cut(s)	1106
Cfr10I	RCCGGY	1 cut(s)	950
Cfr13I	GGNCC	3 cut(s)	152, 408, 830
Csp6I	GTAC	2 cut(s)	68, 262
CviQI	GTAC	2 cut(s)	68, 262
DdeI	CTNAG	3 cut(s)	353, 396, 510
DpnI	GATC	4 cut(s)	61, 551, 620, 1105
DpnII	GATC	4 cut(s)	59, 549, 618, 1103
EciI	GGCGGA	1 cut(s)	72
Eco130I	CCWWGG	3 cut(s)	138, 421, 657
Eco147I	AGGCCT	1 cut(s)	187
Eco47I	GGWCC	3 cut(s)	152, 408, 830
Eco57I	CTGAAG	1 cut(s)	71
Eco88I	CYCGRG	2 cut(s)	236, 384
EcoO109I	RGGNCCY	1 cut(s)	408
EcoRII	CCWGG	1 cut(s)	609
EcoT14I	CCWWGG	3 cut(s)	138, 421, 657
ErhI	CCWWGG	3 cut(s)	138, 421, 657
FaqI	GGGAC	3 cut(s)	253, 421, 843
FblI	GTMKAC	2 cut(s)	630, 765
Fnu4HI	GCNGC	3 cut(s)	210, 680, 922
FokI	GGATG	3 cut(s)	233, 312, 370
Fsp4HI	GCNGC	3 cut(s)	210, 680, 922
FspBI	CTAG	2 cut(s)	338, 711
GluI	GCNGC	3 cut(s)	210, 680, 922
GsuI	CTGGAG	2 cut(s)	593, 755
HaeIII	GGCC	2 cut(s)	187, 954
HapII	CCGG	2 cut(s)	951, 1082
HincII	GTYRAC	1 cut(s)	756
HindII	GTYRAC	1 cut(s)	756
HinfI	GANTC	5 cut(s)	96, 512, 526, 568, 715
HpaI	GTTAAC	1 cut(s)	756
HpaII	CCGG	2 cut(s)	951, 1082
HphI	GGTGA	1 cut(s)	433
Hpy166II	GTNNAC	5 cut(s)	121, 631, 756, 766, 857
Hpy188I	TCNGA	3 cut(s)	105, 573, 780
Hpy188III	TCNNGA	6 cut(s)	89, 238, 355, 406, 448, 1107
Hpy8I	GTNNAC	5 cut(s)	121, 631, 756, 766, 857
HpyAV	CCTTC	5 cut(s)	86, 95, 198, 422, 454
HpyCH4III	ACNGT	2 cut(s)	295, 989
HpyCH4IV	ACGT	2 cut(s)	66, 332
HpyCH4V	TGCA	9 cut(s)	212, 230, 290, 587, 596, 674, 682, 805, 816
HpyF10VI	GCNNNNNNNGC	4 cut(s)	275, 593, 960, 1004
HpyF3I	CTNAG	3 cut(s)	353, 396, 510
HpySE526I	ACGT	2 cut(s)	66, 332
KflI	GGGWCCC	1 cut(s)	408
KroI	GCCGGC	1 cut(s)	950
KroNI	GCCGGC	1 cut(s)	952
KspAI	GTTAAC	1 cut(s)	756
Kzo9I	GATC	4 cut(s)	59, 549, 618, 1103
LmnI	GCTCC	3 cut(s)	643, 904, 932
Lsp1109I	GCAGC	3 cut(s)	196, 666, 933
LweI	GCATC	3 cut(s)	239, 683, 825
MaeI	CTAG	2 cut(s)	338, 711
MaeII	ACGT	2 cut(s)	66, 332
MaeIII	GTNAC	4 cut(s)	578, 645, 821, 1072
MalI	GATC	4 cut(s)	61, 551, 620, 1105
MboI	GATC	4 cut(s)	59, 549, 618, 1103
MboII	GAAGA	3 cut(s)	103, 341, 678
MfeI	CAATTG	1 cut(s)	891
MhlI	GDGCHC	1 cut(s)	937
MluCI	AATT	4 cut(s)	16, 365, 891, 1065
MlyI	GAGTC	2 cut(s)	90, 521
MmeI	TCCRAC	1 cut(s)	277
MnlI	CCTC	5 cut(s)	177, 380, 449, 617, 972
MroNI	GCCGGC	1 cut(s)	950
MseI	TTAA	2 cut(s)	255, 755
MspA1I	CMGCKG	1 cut(s)	46
MspI	CCGG	2 cut(s)	951, 1082
MspR9I	CCNGG	1 cut(s)	611
MunI	CAATTG	1 cut(s)	891
MvaI	CCWGG	1 cut(s)	611
MvnI	CGCG	1 cut(s)	902
MwoI	GCNNNNNNNGC	4 cut(s)	275, 593, 960, 1004
NaeI	GCCGGC	1 cut(s)	952
NcoI	CCATGG	2 cut(s)	138, 657
NdeII	GATC	4 cut(s)	59, 549, 618, 1103
NgoMIV	GCCGGC	1 cut(s)	950
NlaIV	GGNNCC	4 cut(s)	409, 410, 743, 831
NmeAIII	GCCGAG	1 cut(s)	5
NmuCI	GTSAC	2 cut(s)	645, 821
NspI	RCATGY	2 cut(s)	131, 536
PaeR7I	CTCGAG	1 cut(s)	384
PagI	TCATGA	1 cut(s)	1106
PceI	AGGCCT	1 cut(s)	187
PdiI	GCCGGC	1 cut(s)	952
PfeI	GAWTC	3 cut(s)	526, 568, 715
PkrI	GCNGC	3 cut(s)	211, 681, 923
PleI	GAGTC	2 cut(s)	90, 520
PpsI	GAGTC	2 cut(s)	90, 520
PpuMI	RGGWCCY	1 cut(s)	408
Psp5II	RGGWCCY	1 cut(s)	408
Psp6I	CCWGG	1 cut(s)	609
PspGI	CCWGG	1 cut(s)	609
PspN4I	GGNNCC	4 cut(s)	409, 410, 743, 831
PspPI	GGNCC	3 cut(s)	152, 408, 830
PspPPI	RGGWCCY	1 cut(s)	408
PspXI	VCTCGAGB	1 cut(s)	384
PvuII	CAGCTG	1 cut(s)	46
RsaI	GTAC	2 cut(s)	69, 263
RsaNI	GTAC	2 cut(s)	68, 262
SaqAI	TTAA	2 cut(s)	255, 755
SatI	GCNGC	3 cut(s)	210, 680, 922
Sau3AI	GATC	4 cut(s)	59, 549, 618, 1103
Sau96I	GGNCC	3 cut(s)	152, 408, 830
SchI	GAGTC	2 cut(s)	90, 521
ScrFI	CCNGG	1 cut(s)	611
SduI	GDGCHC	1 cut(s)	937
SfaNI	GCATC	3 cut(s)	239, 683, 825
SfcI	CTRYAG	1 cut(s)	516
Sfr274I	CTCGAG	1 cut(s)	384
SinI	GGWCC	3 cut(s)	152, 408, 830
SlaI	CTCGAG	1 cut(s)	384
SmlI	CTYRAG	3 cut(s)	307, 384, 746
SmoI	CTYRAG	3 cut(s)	307, 384, 746
Sse9I	AATT	4 cut(s)	16, 365, 891, 1065
SseBI	AGGCCT	1 cut(s)	187
SsiI	CCGC	3 cut(s)	57, 426, 902
SspMI	CTAG	2 cut(s)	338, 711
StuI	AGGCCT	1 cut(s)	187
StyD4I	CCNGG	1 cut(s)	609
StyI	CCWWGG	3 cut(s)	138, 421, 657
TaaI	ACNGT	2 cut(s)	295, 989
TaiI	ACGT	2 cut(s)	69, 335
TaqI	TCGA	1 cut(s)	385
TasI	AATT	4 cut(s)	16, 365, 891, 1065
TatI	WGTACW	1 cut(s)	261
TfiI	GAWTC	3 cut(s)	526, 568, 715
Tru1I	TTAA	2 cut(s)	255, 755
Tru9I	TTAA	2 cut(s)	255, 755
TscAI	CASTG	4 cut(s)	495, 589, 885, 1093
TseFI	GTSAC	2 cut(s)	645, 821
TseI	GCWGC	3 cut(s)	209, 679, 921
Tsp45I	GTSAC	2 cut(s)	645, 821
TspDTI	ATGAA	3 cut(s)	89, 1073, 1077
TspRI	CASTG	4 cut(s)	495, 589, 885, 1093
VpaK11BI	GGWCC	3 cut(s)	152, 408, 830
XapI	RAATTY	1 cut(s)	1065
XceI	RCATGY	2 cut(s)	131, 536
XhoI	CTCGAG	1 cut(s)	384
XmiI	GTMKAC	2 cut(s)	630, 765
XspI	CTAG	2 cut(s)	338, 711

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.