MD06G1219800.v1.1

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004601
Physical Location & Seq
Reverse (-)
35204313 .. 35204609
297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1219800.v1.1.491

Sequence Viewer

Length: 297 bp
ATGAAGCAGGCATCATATTCTTCCTACATTGCAATTGCACTGTGCACTGTGGTGGTGCTACTGCTGGCCACAGCGCCGGTGTCAACGGCGGTAACATGCAACGCCATTCAACTTAGCCCCTGCGCCGGCGCAATCACCTCTTCAACTCCACCGTCGAAATTATGCTGCAGTAAGATCAAAGAACAGAAGCCCTGCCTCTGCCAGTACCTCAAAAATCCTAACCTGAAGAAGCTTGTCAACACCCCAAATGCCAGGAATGTGGCCAGGACTTGTGGCACTCCCTTCCCCAAGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

99

Amino Acids

10.36

Weight (kDa)

9.42

Isoelectric Point (pI)

28.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 36 - 98 1.2e-09 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 89
AcoI YGGCCR 2 cut(s) 66, 261
AcuI CTGAAG 1 cut(s) 245
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 1 cut(s) 125
AgsI TTSAA 2 cut(s) 110, 144
AjnI CCWGG 2 cut(s) 251, 263
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 1 cut(s) 232
AluI AGCT 1 cut(s) 232
Alw21I GWGCWC 1 cut(s) 47
Alw44I GTGCAC 1 cut(s) 43
AoxI GGCC 2 cut(s) 66, 261
ApaLI GTGCAC 1 cut(s) 43
ApeKI GCWGC 1 cut(s) 165
AspLEI GCGC 3 cut(s) 76, 125, 131
AsuHPI GGTGA 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 47
BalI TGGCCA 2 cut(s) 68, 263
Bbv12I GWGCWC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 152
BceAI ACGGC 1 cut(s) 102
BciT130I CCWGG 2 cut(s) 253, 265
BfaI CTAG 1 cut(s) 295
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 1 cut(s) 77
BisI GCNGC 1 cut(s) 166
BlsI GCNGC 1 cut(s) 167
Bme1390I CCNGG 2 cut(s) 253, 265
BmrFI CCNGG 2 cut(s) 253, 265
BmsI GCATC 1 cut(s) 20
Bsc4I CCNNNNNNNGG 1 cut(s) 125
Bse118I RCCGGY 2 cut(s) 76, 125
Bse1I ACTGG 1 cut(s) 202
Bse3DI GCAATG 1 cut(s) 27
BseBI CCWGG 2 cut(s) 253, 265
BseLI CCNNNNNNNGG 1 cut(s) 125
BseMI GCAATG 1 cut(s) 27
BseNI ACTGG 1 cut(s) 202
BseSI GKGCMC 1 cut(s) 47
BseXI GCAGC 1 cut(s) 152
BshFI GGCC 2 cut(s) 68, 263
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 2 cut(s) 77, 126
BslI CCNNNNNNNGG 1 cut(s) 125
BsnI GGCC 2 cut(s) 68, 263
Bsp1286I GDGCHC 1 cut(s) 47
Bsp143I GATC 1 cut(s) 174
BspACI CCGC 1 cut(s) 89
BspANI GGCC 2 cut(s) 68, 263
BspMAI CTGCAG 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 2 cut(s) 76, 125
BsrI ACTGG 1 cut(s) 202
BssAI RCCGGY 2 cut(s) 76, 125
BssMI GATC 1 cut(s) 174
Bst2UI CCWGG 2 cut(s) 253, 265
Bst4CI ACNGT 3 cut(s) 42, 49, 153
Bst6I CTCTTC 1 cut(s) 145
BstC8I GCNNGC 3 cut(s) 9, 66, 127
BstDEI CTNAG 1 cut(s) 113
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 3 cut(s) 76, 125, 131
BstKTI GATC 1 cut(s) 177
BstMBI GATC 1 cut(s) 174
BstNI CCWGG 2 cut(s) 253, 265
BstNSI RCATGY 1 cut(s) 99
BstSCI CCNGG 2 cut(s) 251, 263
BstSFI CTRYAG 1 cut(s) 166
BstSLI GKGCMC 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 152
BstXI CCANNNNNNTGG 1 cut(s) 259
BsuRI GGCC 2 cut(s) 68, 263
BtsIMutI CAGTG 2 cut(s) 38, 45
Cac8I GCNNGC 3 cut(s) 9, 66, 127
CfoI GCGC 3 cut(s) 76, 125, 131
Cfr10I RCCGGY 2 cut(s) 76, 125
Csp6I GTAC 1 cut(s) 205
CviAII CATG 1 cut(s) 96
CviJI RGCY 5 cut(s) 68, 117, 190, 232, 263
CviKI_1 RGCY 5 cut(s) 68, 117, 190, 232, 263
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 1 cut(s) 113
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
EaeI YGGCCR 2 cut(s) 66, 261
Eam1104I CTCTTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 251, 263
FaeI CATG 1 cut(s) 99
FaiI YATR 3 cut(s) 16, 97, 163
FatI CATG 1 cut(s) 95
Fnu4HI GCNGC 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 166
FspBI CTAG 1 cut(s) 295
GlaI GCGC 3 cut(s) 75, 124, 130
GluI GCNGC 1 cut(s) 166
HaeII RGCGCY 1 cut(s) 77
HaeIII GGCC 2 cut(s) 68, 263
HapII CCGG 2 cut(s) 77, 126
HhaI GCGC 3 cut(s) 76, 125, 131
Hin1II CATG 1 cut(s) 99
Hin6I GCGC 3 cut(s) 74, 123, 129
HinP1I GCGC 3 cut(s) 74, 123, 129
HincII GTYRAC 2 cut(s) 84, 238
HindII GTYRAC 2 cut(s) 84, 238
HindIII AAGCTT 1 cut(s) 230
HpaII CCGG 2 cut(s) 77, 126
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 3 cut(s) 45, 84, 238
Hpy8I GTNNAC 3 cut(s) 45, 84, 238
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 1 cut(s) 292
HpyCH4III ACNGT 3 cut(s) 42, 49, 153
HpyCH4V TGCA 5 cut(s) 32, 38, 45, 99, 168
HpyF3I CTNAG 1 cut(s) 113
Hsp92II CATG 1 cut(s) 99
HspAI GCGC 3 cut(s) 74, 123, 129
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Kzo9I GATC 1 cut(s) 174
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 1 cut(s) 20
MaeI CTAG 1 cut(s) 295
MaeIII GTNAC 1 cut(s) 91
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MboII GAAGA 3 cut(s) 12, 132, 238
MfeI CAATTG 1 cut(s) 33
MhlI GDGCHC 1 cut(s) 47
MlsI TGGCCA 2 cut(s) 68, 263
MluCI AATT 2 cut(s) 33, 158
MluNI TGGCCA 2 cut(s) 68, 263
MnlI CCTC 3 cut(s) 148, 206, 218
Mox20I TGGCCA 2 cut(s) 68, 263
MreI CGCCGGCG 1 cut(s) 125
MroNI GCCGGC 1 cut(s) 125
MscI TGGCCA 2 cut(s) 68, 263
MslI CAYNNNNRTG 1 cut(s) 50
Msp20I TGGCCA 2 cut(s) 68, 263
MspI CCGG 2 cut(s) 77, 126
MspR9I CCNGG 2 cut(s) 253, 265
MunI CAATTG 1 cut(s) 33
MvaI CCWGG 2 cut(s) 253, 265
NaeI GCCGGC 1 cut(s) 127
NdeII GATC 1 cut(s) 174
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 1 cut(s) 99
NspI RCATGY 1 cut(s) 99
OliI CACNNNNGTG 1 cut(s) 50
PdiI GCCGGC 1 cut(s) 127
PkrI GCNGC 1 cut(s) 167
Psp6I CCWGG 2 cut(s) 251, 263
PspGI CCWGG 2 cut(s) 251, 263
PstI CTGCAG 1 cut(s) 170
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 50
SatI GCNGC 1 cut(s) 166
Sau3AI GATC 1 cut(s) 174
ScrFI CCNGG 2 cut(s) 253, 265
SduI GDGCHC 1 cut(s) 47
SetI ASST 4 cut(s) 140, 210, 225, 234
SfaNI GCATC 1 cut(s) 20
SfcI CTRYAG 1 cut(s) 166
SgrAI CRCCGGYG 2 cut(s) 76, 125
SmiMI CAYNNNNRTG 1 cut(s) 50
Sse9I AATT 2 cut(s) 33, 158
SsiI CCGC 1 cut(s) 89
SspMI CTAG 1 cut(s) 295
StyD4I CCNGG 2 cut(s) 251, 263
TaaI ACNGT 3 cut(s) 42, 49, 153
TaqI TCGA 1 cut(s) 155
TasI AATT 2 cut(s) 33, 158
TscAI CASTG 2 cut(s) 45, 52
TseI GCWGC 1 cut(s) 165
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 45, 52
VneI GTGCAC 1 cut(s) 43
XceI RCATGY 1 cut(s) 99
XspI CTAG 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.