MD16G1037400.v1.1

Non-specific lipid-transfer protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004601
Physical Location & Seq
Forward (+)
2696653 .. 2696952
300 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1037400.v1.1.491

Sequence Viewer

Length: 300 bp
ATGATGAGATCAATGTCTTACTACAACGTTGGAATATTGTGTCTTGTAGTTTTTCTTGTTGTGGTCAGGGAAACCCAGGTGGGCGAGGCAGCCGTGACTTGTAACCCAATGGAGCTAGCCCCATGCGCTGCCGCGATCATGTCTTCGAACTCTCCCACCGCCGTGTGTTGCGGAAAACTCAAGGAGCAGAGCCCTTGCCTCTGCCAGTATGTGAAGAACCCCAACCTCCAGAAGCTTGTCAACTCCCCAAACGCCAAGAAGGTCGCCGACACTTGTGGTTCCCCATTCCCCACATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

100

Amino Acids

10.58

Weight (kDa)

8.4

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 134
AciI CCGC 3 cut(s) 132, 159, 171
AclI AACGTT 1 cut(s) 27
AjnI CCWGG 1 cut(s) 75
AleI CACNNNNGTG 1 cut(s) 161
AluBI AGCT 2 cut(s) 115, 235
AluI AGCT 2 cut(s) 115, 235
ApeKI GCWGC 2 cut(s) 89, 128
AspLEI GCGC 1 cut(s) 128
AsuII TTCGAA 1 cut(s) 146
AsuNHI GCTAGC 1 cut(s) 115
BanII GRGCYC 1 cut(s) 194
BbsI GAAGAC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 101, 115
BceAI ACGGC 2 cut(s) 77, 146
BciT130I CCWGG 1 cut(s) 77
BfaI CTAG 1 cut(s) 116
BisI GCNGC 3 cut(s) 90, 129, 132
BlsI GCNGC 3 cut(s) 91, 130, 133
Bme1390I CCNGG 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 77
BmtI GCTAGC 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 135
BpmI CTGGAG 1 cut(s) 212
Bpu14I TTCGAA 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 75
Bse1I ACTGG 1 cut(s) 205
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 205
BseXI GCAGC 2 cut(s) 101, 115
Bsh1236I CGCG 1 cut(s) 134
Bsp119I TTCGAA 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 194
Bsp143I GATC 2 cut(s) 8, 135
BspACI CCGC 3 cut(s) 132, 159, 171
BspFNI CGCG 1 cut(s) 134
BspLI GGNNCC 1 cut(s) 280
BspOI GCTAGC 1 cut(s) 119
BspT104I TTCGAA 1 cut(s) 146
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 1 cut(s) 75
BssMI GATC 2 cut(s) 8, 135
Bst2UI CCWGG 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 117
BstFNI CGCG 1 cut(s) 134
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 2 cut(s) 11, 138
BstMBI GATC 2 cut(s) 8, 135
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNI CCWGG 1 cut(s) 77
BstNSI RCATGY 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 75
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 2 cut(s) 101, 115
BstV2I GAAGAC 1 cut(s) 135
Cac8I GCNNGC 1 cut(s) 117
CfoI GCGC 1 cut(s) 128
CviAII CATG 3 cut(s) 123, 139, 294
CviJI RGCY 5 cut(s) 92, 115, 119, 192, 235
CviKI_1 RGCY 5 cut(s) 92, 115, 119, 192, 235
DpnI GATC 2 cut(s) 10, 137
DpnII GATC 2 cut(s) 8, 135
Eco24I GRGCYC 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 75
EcoT38I GRGCYC 1 cut(s) 194
FaeI CATG 3 cut(s) 126, 142, 297
FaiI YATR 4 cut(s) 124, 140, 210, 295
FatI CATG 3 cut(s) 122, 138, 293
Fnu4HI GCNGC 3 cut(s) 90, 129, 132
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 3 cut(s) 90, 129, 132
FspBI CTAG 1 cut(s) 116
GlaI GCGC 1 cut(s) 127
GluI GCNGC 3 cut(s) 90, 129, 132
GsuI CTGGAG 1 cut(s) 212
HhaI GCGC 1 cut(s) 128
Hin1II CATG 3 cut(s) 126, 142, 297
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HindIII AAGCTT 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 241
Hpy188III TCNNGA 1 cut(s) 229
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 1 cut(s) 253
HpyCH4IV ACGT 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 3 cut(s) 126, 142, 297
HspAI GCGC 1 cut(s) 126
Kzo9I GATC 2 cut(s) 8, 135
LmnI GCTCC 2 cut(s) 112, 184
LpnPI CCDG 5 cut(s) 52, 62, 89, 218, 242
Lsp1109I GCAGC 2 cut(s) 101, 115
MaeI CTAG 1 cut(s) 116
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 94, 101
MalI GATC 2 cut(s) 10, 137
MboI GATC 2 cut(s) 8, 135
MboII GAAGA 2 cut(s) 135, 226
MhlI GDGCHC 1 cut(s) 194
MmeI TCCRAC 1 cut(s) 10
MnlI CCTC 3 cut(s) 79, 209, 236
MslI CAYNNNNRTG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
MvnI CGCG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 125
NdeII GATC 2 cut(s) 8, 135
NheI GCTAGC 1 cut(s) 115
NlaIII CATG 3 cut(s) 126, 142, 297
NlaIV GGNNCC 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 94
NspI RCATGY 1 cut(s) 297
NspV TTCGAA 1 cut(s) 146
OliI CACNNNNGTG 1 cut(s) 161
PkrI GCNGC 3 cut(s) 91, 130, 133
Psp1406I AACGTT 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 280
RseI CAYNNNNRTG 1 cut(s) 161
SatI GCNGC 3 cut(s) 90, 129, 132
Sau3AI GATC 2 cut(s) 8, 135
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 1 cut(s) 194
SetI ASST 6 cut(s) 30, 81, 117, 228, 237, 264
SfuI TTCGAA 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SsiI CCGC 3 cut(s) 132, 159, 171
SspI AATATT 1 cut(s) 36
SspMI CTAG 1 cut(s) 116
StyD4I CCNGG 1 cut(s) 75
TaiI ACGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 146
TauI GCSGC 1 cut(s) 134
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 2 cut(s) 89, 128
Tsp45I GTSAC 1 cut(s) 94
XceI RCATGY 1 cut(s) 297
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.