RchiOBHm_Chr7g0179461

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004601
Physical Location & Seq
Forward (+)
1711529 .. 1712240
712 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15997

Sequence Viewer

Length: 429 bp
ATGTTATTCTACTATATTTCCTCTTCTATAAATTGGGAGGGCACCCAAGGCAAACGCATCACTCAGCGCACACAACACTACCCACTTAACACTATCTCATTCTCTCTTTCTTTGAGGTTTGACATTGTAACACTGAAAATGAAGGCATCATATGTCGCAATTGCATTGTGCACTGTGGTTGTGGTGATGCTGGCCACAGCAGAGGTGTCCATGGCAGCTGTGACTTGCAGCCCAGTAGAGCTGAGCTCCTGTGCAAGTGCAATAAGCTCTAATACTCCACCATCAAATCTATGCTGCACCAAAATCAAGGAGCAGAAGCCTTGTCTCTGCCAGTACCTCAACAACCCCAACCTCAAGAAATTCGTTAACACCCCGAATGCTCGGAGAGTTGCTAGGACTTGTGGCACTCCCTTCCCCAGGTGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

142

Amino Acids

15.65

Weight (kDa)

9.39

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 80 - 141 1.6e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 1 cut(s) 359
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 1 cut(s) 417
AjnI CCWGG 1 cut(s) 416
AluBI AGCT 5 cut(s) 218, 241, 246, 267, 426
AluI AGCT 5 cut(s) 218, 241, 246, 267, 426
Alw21I GWGCWC 2 cut(s) 173, 248
Alw26I GTCTC 1 cut(s) 329
Alw44I GTGCAC 1 cut(s) 169
AoxI GGCC 1 cut(s) 192
ApaLI GTGCAC 1 cut(s) 169
ApeKI GCWGC 4 cut(s) 215, 228, 294, 423
ApoI RAATTY 1 cut(s) 359
AspLEI GCGC 1 cut(s) 69
AsuHPI GGTGA 1 cut(s) 196
BaeGI GKGCMC 2 cut(s) 44, 173
BalI TGGCCA 1 cut(s) 194
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 248
Bbv12I GWGCWC 2 cut(s) 173, 248
BbvI GCAGC 3 cut(s) 227, 240, 281
BccI CCATC 1 cut(s) 289
BciT130I CCWGG 1 cut(s) 418
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 2 cut(s) 393, 427
BisI GCNGC 4 cut(s) 216, 229, 295, 424
BlpI GCTNAGC 1 cut(s) 242
BlsI GCNGC 4 cut(s) 217, 230, 296, 425
Bme1390I CCNGG 1 cut(s) 418
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 1 cut(s) 418
BmrI ACTGGG 1 cut(s) 227
BmsI GCATC 3 cut(s) 66, 155, 177
BmuI ACTGGG 1 cut(s) 227
BplI GAGNNNNNCTC 2 cut(s) 230, 262
Bpu1102I GCTNAGC 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 338
BsaJI CCNNGG 3 cut(s) 46, 210, 416
Bsc4I CCNNNNNNNGG 1 cut(s) 417
Bse1I ACTGG 2 cut(s) 233, 331
BseBI CCWGG 1 cut(s) 418
BseDI CCNNGG 3 cut(s) 46, 210, 416
BseLI CCNNNNNNNGG 1 cut(s) 417
BseMII CTCAG 2 cut(s) 77, 233
BseNI ACTGG 2 cut(s) 233, 331
BseSI GKGCMC 2 cut(s) 44, 173
BseXI GCAGC 3 cut(s) 227, 240, 281
BsgI GTGCAG 1 cut(s) 280
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 41
BsiHKAI GWGCWC 2 cut(s) 173, 248
BslI CCNNNNNNNGG 1 cut(s) 417
BsmAI GTCTC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 382
BsnI GGCC 1 cut(s) 194
Bsp1286I GDGCHC 3 cut(s) 44, 173, 248
Bsp1720I GCTNAGC 1 cut(s) 242
Bsp19I CCATGG 1 cut(s) 210
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 2 cut(s) 76, 234
BspLI GGNNCC 1 cut(s) 43
BspT107I GGYRCC 1 cut(s) 41
BsrI ACTGG 2 cut(s) 233, 331
BssECI CCNNGG 3 cut(s) 46, 210, 416
BssT1I CCWWGG 2 cut(s) 46, 210
Bst2UI CCWGG 1 cut(s) 418
Bst4CI ACNGT 1 cut(s) 175
Bst6I CTCTTC 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 2 cut(s) 63, 242
BstDSI CCRYGG 1 cut(s) 210
BstENI CCTNNNNNAGG 1 cut(s) 415
BstHHI GCGC 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 329
BstMWI GCNNNNNNNGC 1 cut(s) 48
BstNI CCWGG 1 cut(s) 418
BstSCI CCNGG 1 cut(s) 416
BstSLI GKGCMC 2 cut(s) 44, 173
BstV1I GCAGC 3 cut(s) 227, 240, 281
BsuRI GGCC 1 cut(s) 194
BtgI CCRYGG 1 cut(s) 210
BtsIMutI CAGTG 2 cut(s) 131, 171
Cac8I GCNNGC 1 cut(s) 192
CfoI GCGC 1 cut(s) 69
Csp6I GTAC 1 cut(s) 334
CviAII CATG 1 cut(s) 211
CviJI RGCY 8 cut(s) 194, 218, 231, 241, 246, 267, 319, 426
CviKI_1 RGCY 8 cut(s) 194, 218, 231, 241, 246, 267, 319, 426
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 2 cut(s) 63, 242
EaeI YGGCCR 1 cut(s) 192
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 246
Eco130I CCWWGG 2 cut(s) 46, 210
Eco24I GRGCYC 1 cut(s) 248
Eco53kI GAGCTC 1 cut(s) 246
EcoICRI GAGCTC 1 cut(s) 246
EcoNI CCTNNNNNAGG 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 416
EcoT14I CCWWGG 2 cut(s) 46, 210
EcoT38I GRGCYC 1 cut(s) 248
ErhI CCWWGG 2 cut(s) 46, 210
FaeI CATG 1 cut(s) 214
FaiI YATR 6 cut(s) 15, 29, 151, 153, 212, 292
FatI CATG 1 cut(s) 210
FauNDI CATATG 1 cut(s) 151
Fnu4HI GCNGC 4 cut(s) 216, 229, 295, 424
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 4 cut(s) 216, 229, 295, 424
FspBI CTAG 2 cut(s) 393, 427
GlaI GCGC 1 cut(s) 68
GluI GCNGC 4 cut(s) 216, 229, 295, 424
HaeIII GGCC 1 cut(s) 194
HhaI GCGC 1 cut(s) 69
Hin1II CATG 1 cut(s) 214
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HpaI GTTAAC 1 cut(s) 367
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 2 cut(s) 171, 367
Hpy188I TCNGA 1 cut(s) 384
Hpy188III TCNNGA 1 cut(s) 355
Hpy8I GTNNAC 2 cut(s) 171, 367
HpyAV CCTTC 2 cut(s) 136, 421
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4V TGCA 7 cut(s) 164, 171, 228, 254, 260, 297, 423
HpyF10VI GCNNNNNNNGC 1 cut(s) 48
HpyF3I CTNAG 2 cut(s) 63, 242
Hsp92II CATG 1 cut(s) 214
HspAI GCGC 1 cut(s) 67
KspAI GTTAAC 1 cut(s) 367
LmnI GCTCC 2 cut(s) 251, 310
LpnPI CCDG 5 cut(s) 176, 246, 262, 344, 403
Lsp1109I GCAGC 3 cut(s) 227, 240, 281
LweI GCATC 3 cut(s) 66, 155, 177
MaeI CTAG 2 cut(s) 393, 427
MaeIII GTNAC 2 cut(s) 127, 220
MboII GAAGA 1 cut(s) 15
MfeI CAATTG 1 cut(s) 159
MhlI GDGCHC 3 cut(s) 44, 173, 248
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 3 cut(s) 31, 159, 359
MluNI TGGCCA 1 cut(s) 194
MnlI CCTC 6 cut(s) 31, 31, 108, 196, 347, 362
Mox20I TGGCCA 1 cut(s) 194
MscI TGGCCA 1 cut(s) 194
MseI TTAA 2 cut(s) 87, 366
Msp20I TGGCCA 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 218
MspR9I CCNGG 1 cut(s) 418
MunI CAATTG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 382
MvaI CCWGG 1 cut(s) 418
MwoI GCNNNNNNNGC 1 cut(s) 48
NcoI CCATGG 1 cut(s) 210
NdeI CATATG 1 cut(s) 151
NlaIII CATG 1 cut(s) 214
NlaIV GGNNCC 1 cut(s) 43
NmuCI GTSAC 1 cut(s) 220
PctI GAATGC 1 cut(s) 382
PkrI GCNGC 4 cut(s) 217, 230, 296, 425
Psp124BI GAGCTC 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 416
PspGI CCWGG 1 cut(s) 416
PspN4I GGNNCC 1 cut(s) 43
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SacI GAGCTC 1 cut(s) 248
SaqAI TTAA 2 cut(s) 87, 366
SatI GCNGC 4 cut(s) 216, 229, 295, 424
ScrFI CCNGG 1 cut(s) 418
SduI GDGCHC 3 cut(s) 44, 173, 248
SfaNI GCATC 3 cut(s) 66, 155, 177
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 3 cut(s) 31, 159, 359
SspMI CTAG 2 cut(s) 393, 427
SstI GAGCTC 1 cut(s) 248
StyD4I CCNGG 1 cut(s) 416
StyI CCWWGG 2 cut(s) 46, 210
TaaI ACNGT 1 cut(s) 175
TasI AATT 3 cut(s) 31, 159, 359
Tru1I TTAA 2 cut(s) 87, 366
Tru9I TTAA 2 cut(s) 87, 366
TscAI CASTG 2 cut(s) 138, 178
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 4 cut(s) 215, 228, 294, 423
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 155
TspRI CASTG 2 cut(s) 138, 178
VneI GTGCAC 1 cut(s) 169
XagI CCTNNNNNAGG 1 cut(s) 415
XapI RAATTY 1 cut(s) 359
XspI CTAG 2 cut(s) 393, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.