MD14G1229500.v1.1

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004601
Physical Location & Seq
Reverse (-)
31061243 .. 31061536
294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1229500.v1.1.491

Sequence Viewer

Length: 294 bp
ATGAAGGCATCATATTCTTCCTACATTGCAATTGCATTGTGCACTGTGTTGGTGCTGCTGCTGGCCGCAGCGCCGGTGTCAACGGCGGTAACATGCAACGCCATTCAACTTAGCCCCTGCGCCGGAGCAATCACCTCTTCAACTCCACCGTCGACATTATGCTGCAGCAAGATCAAGGAACAGAAGCCCTGCCTCTGCCAGTACCTCAAGAACCCTAACCTCAAGAAGCTTGTCAACACCCCAAATGCGAGGAAAGTTGCCAGCACCTGTGGCACTCCCTTCCCTAAGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

98

Amino Acids

10.14

Weight (kDa)

9.3

Isoelectric Point (pI)

24.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 35 - 97 8.4e-10 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 152
AciI CCGC 2 cut(s) 66, 86
AcoI YGGCCR 1 cut(s) 63
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 122
AgsI TTSAA 2 cut(s) 107, 141
AluBI AGCT 1 cut(s) 229
AluI AGCT 1 cut(s) 229
Alw21I GWGCWC 1 cut(s) 44
Alw44I GTGCAC 1 cut(s) 40
AlwNI CAGNNNCTG 1 cut(s) 267
AoxI GGCC 1 cut(s) 63
ApaLI GTGCAC 1 cut(s) 40
ApeKI GCWGC 5 cut(s) 55, 58, 68, 162, 165
AspLEI GCGC 2 cut(s) 73, 122
AsuHPI GGTGA 1 cut(s) 124
BaeGI GKGCMC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 5 cut(s) 42, 45, 80, 149, 177
BceAI ACGGC 1 cut(s) 99
BfaI CTAG 1 cut(s) 292
BfmI CTRYAG 1 cut(s) 163
BfoI RGCGCY 1 cut(s) 74
BisI GCNGC 6 cut(s) 56, 59, 66, 69, 163, 166
BlsI GCNGC 6 cut(s) 57, 60, 67, 70, 164, 167
BmsI GCATC 1 cut(s) 17
BpuEI CTTGAG 2 cut(s) 191, 206
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse118I RCCGGY 1 cut(s) 73
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMI GCAATG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 199
BseSI GKGCMC 1 cut(s) 44
BseXI GCAGC 5 cut(s) 42, 45, 80, 149, 177
BshFI GGCC 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 44
BsiSI CCGG 2 cut(s) 74, 123
BslI CCNNNNNNNGG 1 cut(s) 122
BsnI GGCC 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 1 cut(s) 171
BspACI CCGC 2 cut(s) 66, 86
BspANI GGCC 1 cut(s) 65
BspMAI CTGCAG 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 24
BsrFI RCCGGY 1 cut(s) 73
BsrI ACTGG 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 73
BssMI GATC 1 cut(s) 171
Bst4CI ACNGT 2 cut(s) 46, 150
Bst6I CTCTTC 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 63, 262
BstDEI CTNAG 2 cut(s) 110, 285
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 2 cut(s) 73, 122
BstKTI GATC 1 cut(s) 174
BstMBI GATC 1 cut(s) 171
BstMWI GCNNNNNNNGC 1 cut(s) 270
BstNSI RCATGY 1 cut(s) 96
BstSFI CTRYAG 1 cut(s) 163
BstSLI GKGCMC 1 cut(s) 44
BstV1I GCAGC 5 cut(s) 42, 45, 80, 149, 177
BsuRI GGCC 1 cut(s) 65
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 2 cut(s) 63, 262
CaiI CAGNNNCTG 1 cut(s) 267
CfoI GCGC 2 cut(s) 73, 122
Cfr10I RCCGGY 1 cut(s) 73
Csp6I GTAC 1 cut(s) 202
CviAII CATG 1 cut(s) 93
CviJI RGCY 4 cut(s) 65, 114, 187, 229
CviKI_1 RGCY 4 cut(s) 65, 114, 187, 229
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 2 cut(s) 110, 285
DpnI GATC 1 cut(s) 173
DpnII GATC 1 cut(s) 171
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
FaeI CATG 1 cut(s) 96
FaiI YATR 3 cut(s) 13, 94, 160
FatI CATG 1 cut(s) 92
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 6 cut(s) 56, 59, 66, 69, 163, 166
Fsp4HI GCNGC 6 cut(s) 56, 59, 66, 69, 163, 166
FspBI CTAG 1 cut(s) 292
GlaI GCGC 2 cut(s) 72, 121
GluI GCNGC 6 cut(s) 56, 59, 66, 69, 163, 166
HaeII RGCGCY 1 cut(s) 74
HaeIII GGCC 1 cut(s) 65
HapII CCGG 2 cut(s) 74, 123
HhaI GCGC 2 cut(s) 73, 122
Hin1II CATG 1 cut(s) 96
Hin6I GCGC 2 cut(s) 71, 120
HinP1I GCGC 2 cut(s) 71, 120
HincII GTYRAC 3 cut(s) 81, 153, 235
HindII GTYRAC 3 cut(s) 81, 153, 235
HindIII AAGCTT 1 cut(s) 227
HpaII CCGG 2 cut(s) 74, 123
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 4 cut(s) 42, 81, 153, 235
Hpy188III TCNNGA 2 cut(s) 208, 223
Hpy8I GTNNAC 4 cut(s) 42, 81, 153, 235
Hpy99I CGWCG 1 cut(s) 154
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 2 cut(s) 46, 150
HpyCH4V TGCA 5 cut(s) 29, 35, 42, 96, 165
HpyF10VI GCNNNNNNNGC 1 cut(s) 270
HpyF3I CTNAG 2 cut(s) 110, 285
Hsp92II CATG 1 cut(s) 96
HspAI GCGC 2 cut(s) 71, 120
Kzo9I GATC 1 cut(s) 171
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 8 cut(s) 47, 87, 130, 136, 202, 212, 274, 280
Lsp1109I GCAGC 5 cut(s) 42, 45, 80, 149, 177
LweI GCATC 1 cut(s) 17
MaeI CTAG 1 cut(s) 292
MaeIII GTNAC 1 cut(s) 88
MalI GATC 1 cut(s) 173
MboI GATC 1 cut(s) 171
MboII GAAGA 2 cut(s) 9, 129
MfeI CAATTG 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 44
MluCI AATT 1 cut(s) 30
MnlI CCTC 5 cut(s) 145, 203, 215, 230, 243
MspI CCGG 2 cut(s) 74, 123
MunI CAATTG 1 cut(s) 30
MwoI GCNNNNNNNGC 1 cut(s) 270
NdeII GATC 1 cut(s) 171
NlaIII CATG 1 cut(s) 96
NspI RCATGY 1 cut(s) 96
PkrI GCNGC 6 cut(s) 57, 60, 67, 70, 164, 167
PstI CTGCAG 1 cut(s) 167
PstNI CAGNNNCTG 1 cut(s) 267
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SalI GTCGAC 1 cut(s) 151
SatI GCNGC 6 cut(s) 56, 59, 66, 69, 163, 166
Sau3AI GATC 1 cut(s) 171
SduI GDGCHC 1 cut(s) 44
SetI ASST 5 cut(s) 137, 207, 222, 231, 269
SfaNI GCATC 1 cut(s) 17
SfcI CTRYAG 1 cut(s) 163
SgrAI CRCCGGYG 1 cut(s) 73
SmlI CTYRAG 2 cut(s) 206, 221
SmoI CTYRAG 2 cut(s) 206, 221
Sse9I AATT 1 cut(s) 30
SsiI CCGC 2 cut(s) 66, 86
SspMI CTAG 1 cut(s) 292
TaaI ACNGT 2 cut(s) 46, 150
TaqI TCGA 1 cut(s) 152
TasI AATT 1 cut(s) 30
TauI GCSGC 1 cut(s) 68
TscAI CASTG 1 cut(s) 49
TseI GCWGC 5 cut(s) 55, 58, 68, 162, 165
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 49
VneI GTGCAC 1 cut(s) 40
XceI RCATGY 1 cut(s) 96
XmiI GTMKAC 1 cut(s) 152
XspI CTAG 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.