MD15G1204900.v1.1

Non-specific lipid-transfer protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004601
Physical Location & Seq
Reverse (-)
16306249 .. 16306593
345 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1204900.v1.1.491

Sequence Viewer

Length: 345 bp
ATGCGTTGGGTGTGGGTTTGTTTAGGAAGCCTTTGTTTGAGGCTATGGCCGAGCAGAACAAAAAGGTGCAATCTGGAGATGAGTCGGATCCGCGAGCTTACAAGAGAGGAAACCCAGGTAGGTGAGGCAGCCGCGACTTGTAACCCAATGGAGCTAGGCCCATGCGTCGCCGCAATTATGTCCTTGAACTCTCCCACCGCCGTGTGTTGCGGAAAACTCAAGGAGCAGAGCCCTTGCCTCTACCAATATGTGAAGAACCCCAACCTCCAAAAGCTTGTCAACTCCTCAAATGCCAAGAAGGTCGCCGACACTTGTGGTTGCCCATTCCCTACATGCAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

115

Amino Acids

12.64

Weight (kDa)

8.95

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 93, 134
AciI CCGC 5 cut(s) 91, 132, 171, 198, 210
AclWI GGATC 2 cut(s) 82, 95
AcoI YGGCCR 1 cut(s) 47
AgsI TTSAA 1 cut(s) 187
AjnI CCWGG 1 cut(s) 114
AleI CACNNNNGTG 1 cut(s) 200
AluBI AGCT 4 cut(s) 97, 154, 274, 341
AluI AGCT 4 cut(s) 97, 154, 274, 341
AlwI GGATC 2 cut(s) 82, 95
AoxI GGCC 2 cut(s) 47, 157
ApeKI GCWGC 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 134
BamHI GGATCC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 233
BbvI GCAGC 1 cut(s) 140
BceAI ACGGC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 116
BfaI CTAG 1 cut(s) 155
BisI GCNGC 3 cut(s) 129, 132, 171
BlsI GCNGC 3 cut(s) 130, 133, 172
Bme1390I CCNGG 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 1 cut(s) 116
BpmI CTGGAG 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 114
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 1 cut(s) 114
BseRI GAGGAG 1 cut(s) 274
BseXI GCAGC 1 cut(s) 140
Bsh1236I CGCG 2 cut(s) 93, 134
BshFI GGCC 2 cut(s) 49, 159
BsnI GGCC 2 cut(s) 49, 159
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 1 cut(s) 87
BspACI CCGC 5 cut(s) 91, 132, 171, 198, 210
BspANI GGCC 2 cut(s) 49, 159
BspFNI CGCG 2 cut(s) 93, 134
BspLI GGNNCC 1 cut(s) 89
BspPI GGATC 2 cut(s) 82, 95
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 116
BstC8I GCNNGC 1 cut(s) 95
BstFNI CGCG 2 cut(s) 93, 134
BstKTI GATC 1 cut(s) 90
BstMBI GATC 1 cut(s) 87
BstNI CCWGG 1 cut(s) 116
BstNSI RCATGY 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 114
BstUI CGCG 2 cut(s) 93, 134
BstV1I GCAGC 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BsuRI GGCC 2 cut(s) 49, 159
Cac8I GCNNGC 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 158
CseI GACGC 1 cut(s) 154
CviAII CATG 2 cut(s) 162, 333
DpnI GATC 1 cut(s) 89
DpnII GATC 1 cut(s) 87
EaeI YGGCCR 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 233
FaeI CATG 2 cut(s) 165, 336
FaiI YATR 5 cut(s) 46, 163, 179, 249, 334
FatI CATG 2 cut(s) 161, 332
Fnu4HI GCNGC 3 cut(s) 129, 132, 171
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 3 cut(s) 129, 132, 171
FspBI CTAG 1 cut(s) 155
GluI GCNGC 3 cut(s) 129, 132, 171
GsuI CTGGAG 1 cut(s) 95
HaeIII GGCC 2 cut(s) 49, 159
HgaI GACGC 1 cut(s) 154
Hin1II CATG 2 cut(s) 165, 336
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 2 cut(s) 272, 339
HinfI GANTC 1 cut(s) 82
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 1 cut(s) 87
Hpy188III TCNNGA 1 cut(s) 74
Hpy8I GTNNAC 1 cut(s) 280
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 1 cut(s) 292
HpyCH4V TGCA 2 cut(s) 69, 336
Hsp92II CATG 2 cut(s) 165, 336
Kzo9I GATC 1 cut(s) 87
LmnI GCTCC 2 cut(s) 151, 223
LpnPI CCDG 3 cut(s) 59, 101, 128
Lsp1109I GCAGC 1 cut(s) 140
MaeI CTAG 1 cut(s) 155
MaeIII GTNAC 1 cut(s) 140
MalI GATC 1 cut(s) 89
MboI GATC 1 cut(s) 87
MboII GAAGA 1 cut(s) 265
MflI RGATCY 1 cut(s) 87
MhlI GDGCHC 1 cut(s) 233
MluCI AATT 1 cut(s) 174
MlyI GAGTC 1 cut(s) 91
MmeI TCCRAC 1 cut(s) 65
MnlI CCTC 6 cut(s) 33, 100, 118, 248, 275, 295
MslI CAYNNNNRTG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 116
MvaI CCWGG 1 cut(s) 116
MvnI CGCG 2 cut(s) 93, 134
NdeII GATC 1 cut(s) 87
NlaIII CATG 2 cut(s) 165, 336
NlaIV GGNNCC 1 cut(s) 89
NmeAIII GCCGAG 1 cut(s) 75
NspI RCATGY 1 cut(s) 336
OliI CACNNNNGTG 1 cut(s) 200
PkrI GCNGC 3 cut(s) 130, 133, 172
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 158
PsuI RGATCY 1 cut(s) 87
RseI CAYNNNNRTG 1 cut(s) 200
SatI GCNGC 3 cut(s) 129, 132, 171
Sau3AI GATC 1 cut(s) 87
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 1 cut(s) 91
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 233
SetI ASST 9 cut(s) 68, 99, 120, 124, 156, 267, 276, 303, 343
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 1 cut(s) 174
SsiI CCGC 5 cut(s) 91, 132, 171, 198, 210
SspMI CTAG 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 114
TasI AATT 1 cut(s) 174
TauI GCSGC 2 cut(s) 134, 173
TseI GCWGC 1 cut(s) 128
XceI RCATGY 1 cut(s) 336
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.