RLG00000005354

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004601
Physical Location & Seq
Reverse (-)
67039591 .. 67040115
525 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005354

Sequence Viewer

Length: 342 bp
ATGAAGGCATCATATGTCGCAATTGCATTATGCACTGTGGTTGTGGTGATGCTGGCCACAGCAGAGGTGTCCATGGCAGCTGTGACTTGCAGCCCAGTAGAGCTGAGCTCCTGTGCAAGTGCAATAAGCTCTAATACTCCACCATCAAATCTATGCTGCACCAAAATCAAGGAGCAGAAGCCTTGTCTCTGCCAGTACCTCAACAACCCCAACCTCAAGAAATTCGTTAACACCCCGAATGCTCGGAGAGTTGCTAGGACTTGTGGCACTCCCTTCCCCAGCATTTTCAGTCTCCTGGTGTTGTGGTCTATCATGAGTAGTATGTATGGTTGTCATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

114

Amino Acids

12.14

Weight (kDa)

8.95

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 34 - 94 1.2e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 221
AfaI GTAC 1 cut(s) 197
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 4 cut(s) 80, 103, 108, 129
AluI AGCT 4 cut(s) 80, 103, 108, 129
Alw21I GWGCWC 1 cut(s) 110
Alw26I GTCTC 2 cut(s) 191, 296
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 3 cut(s) 77, 90, 156
ApoI RAATTY 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 58
BalI TGGCCA 1 cut(s) 56
BanII GRGCYC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 3 cut(s) 89, 102, 143
BccI CCATC 1 cut(s) 151
BciT130I CCWGG 1 cut(s) 296
BcoDI GTCTC 2 cut(s) 191, 296
BfaI CTAG 1 cut(s) 255
BisI GCNGC 3 cut(s) 78, 91, 157
BlpI GCTNAGC 1 cut(s) 104
BlsI GCNGC 3 cut(s) 79, 92, 158
Bme1390I CCNGG 1 cut(s) 296
BmrFI CCNGG 1 cut(s) 296
BmrI ACTGGG 1 cut(s) 89
BmsI GCATC 2 cut(s) 17, 39
BmuI ACTGGG 1 cut(s) 89
BplI GAGNNNNNCTC 2 cut(s) 92, 124
Bpu1102I GCTNAGC 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 72
Bse1I ACTGG 2 cut(s) 95, 193
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 1 cut(s) 72
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 2 cut(s) 95, 193
BseXI GCAGC 3 cut(s) 89, 102, 143
BseYI CCCAGC 1 cut(s) 278
BsgI GTGCAG 1 cut(s) 142
BshFI GGCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 110
BsmAI GTCTC 2 cut(s) 191, 296
BsmI GAATGC 1 cut(s) 244
BsnI GGCC 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 110
Bsp1720I GCTNAGC 1 cut(s) 104
Bsp19I CCATGG 1 cut(s) 72
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 1 cut(s) 96
BspHI TCATGA 1 cut(s) 312
BsrI ACTGG 2 cut(s) 95, 193
BssECI CCNNGG 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 1 cut(s) 104
BstDSI CCRYGG 1 cut(s) 72
BstMAI GTCTC 2 cut(s) 191, 296
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 294
BstV1I GCAGC 3 cut(s) 89, 102, 143
BsuRI GGCC 1 cut(s) 56
BtgI CCRYGG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 33
Cac8I GCNNGC 1 cut(s) 54
CciI TCATGA 1 cut(s) 312
Csp6I GTAC 1 cut(s) 196
CviAII CATG 2 cut(s) 73, 313
CviJI RGCY 7 cut(s) 56, 80, 93, 103, 108, 129, 181
CviKI_1 RGCY 7 cut(s) 56, 80, 93, 103, 108, 129, 181
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 104
EaeI YGGCCR 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 110
Eco53kI GAGCTC 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 110
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 2 cut(s) 76, 316
FaiI YATR 8 cut(s) 13, 15, 31, 74, 154, 314, 323, 327
FatI CATG 2 cut(s) 72, 312
FauNDI CATATG 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 78, 91, 157
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 3 cut(s) 78, 91, 157
FspBI CTAG 1 cut(s) 255
GluI GCNGC 3 cut(s) 78, 91, 157
GsaI CCCAGC 1 cut(s) 282
HaeIII GGCC 1 cut(s) 56
Hin1II CATG 2 cut(s) 76, 316
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HpaI GTTAAC 1 cut(s) 229
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 1 cut(s) 246
Hpy188III TCNNGA 2 cut(s) 217, 313
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 1 cut(s) 283
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4V TGCA 6 cut(s) 26, 33, 90, 116, 122, 159
HpyF3I CTNAG 1 cut(s) 104
Hsp92II CATG 2 cut(s) 76, 316
KspAI GTTAAC 1 cut(s) 229
LmnI GCTCC 2 cut(s) 113, 172
LpnPI CCDG 7 cut(s) 38, 108, 124, 206, 281, 292, 308
Lsp1109I GCAGC 3 cut(s) 89, 102, 143
LweI GCATC 2 cut(s) 17, 39
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 82
MfeI CAATTG 1 cut(s) 21
MhlI GDGCHC 1 cut(s) 110
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 2 cut(s) 21, 221
MluNI TGGCCA 1 cut(s) 56
MnlI CCTC 3 cut(s) 58, 209, 224
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MseI TTAA 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 296
MunI CAATTG 1 cut(s) 21
Mva1269I GAATGC 1 cut(s) 244
MvaI CCWGG 1 cut(s) 296
NcoI CCATGG 1 cut(s) 72
NdeI CATATG 1 cut(s) 13
NlaIII CATG 2 cut(s) 76, 316
NmuCI GTSAC 1 cut(s) 82
PagI TCATGA 1 cut(s) 312
PctI GAATGC 1 cut(s) 244
PkrI GCNGC 3 cut(s) 79, 92, 158
Psp124BI GAGCTC 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 294
PspFI CCCAGC 1 cut(s) 278
PspGI CCWGG 1 cut(s) 294
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SacI GAGCTC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 3 cut(s) 78, 91, 157
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 1 cut(s) 110
SetI ASST 7 cut(s) 69, 82, 105, 110, 131, 201, 216
SfaNI GCATC 2 cut(s) 17, 39
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 2 cut(s) 21, 221
SspMI CTAG 1 cut(s) 255
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 37
TasI AATT 2 cut(s) 21, 221
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 3 cut(s) 77, 90, 156
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 40
XapI RAATTY 1 cut(s) 221
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.