RLG00000005353

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004601
Physical Location & Seq
Reverse (-)
67024702 .. 67025453
752 bp
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UTR
Exon/CDS
Intron
RLM00000005353

Sequence Viewer

Length: 414 bp
ATGAAGGCATCATATTTCGCAATTGCATTATGCTCTGTGCTTGTGGTGATGCTGGCCACAGAAGAGGTGTTGGTGGCAGCTGTGACTTGCAACCCGGTAGAGCTGAGCTCCTGTGCAAATGCAATAAACTCTAATACTCCACCGTCAAATCTATGCTGCACCAAACTCAATCAGCAGAAGCCTTGTCTCTGCCAGTACCTCAACAACCCCAACCTCAAGCAATTCGTTAACAGCCCGAACGCTCGGAATGTTGCTAGGACTTGTGGAGTGCCCTTTCCCAGCCCAATATTACTTATTCTAAAGCTAGTCATAACCTGGCCTAAATTGAGATACCCTTTTCTATTTCTTTCTGATCGCCAAGAGTGCCAATATGGTCTCCAACACAAACAAGACTCAAGACCGACCAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

138

Amino Acids

15.29

Weight (kDa)

9.18

Isoelectric Point (pI)

55.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 34 - 94 5.7e-10 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 54
AfaI GTAC 1 cut(s) 197
AjnI CCWGG 1 cut(s) 314
AluBI AGCT 4 cut(s) 80, 103, 108, 304
AluI AGCT 4 cut(s) 80, 103, 108, 304
Alw21I GWGCWC 1 cut(s) 110
Alw26I GTCTC 2 cut(s) 191, 380
AoxI GGCC 2 cut(s) 54, 317
ApeKI GCWGC 2 cut(s) 77, 156
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 273
BalI TGGCCA 1 cut(s) 56
BanII GRGCYC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 2 cut(s) 89, 143
BciT130I CCWGG 1 cut(s) 316
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 2 cut(s) 191, 380
BfaI CTAG 2 cut(s) 255, 305
BisI GCNGC 2 cut(s) 78, 157
BlpI GCTNAGC 1 cut(s) 104
BlsI GCNGC 2 cut(s) 79, 158
Bme1390I CCNGG 2 cut(s) 95, 316
BmrFI CCNGG 2 cut(s) 95, 316
BmsI GCATC 2 cut(s) 17, 39
BplI GAGNNNNNCTC 2 cut(s) 92, 124
Bpu1102I GCTNAGC 1 cut(s) 104
BpuEI CTTGAG 2 cut(s) 200, 379
BpuMI CCSGG 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 380
Bse1I ACTGG 1 cut(s) 193
BseBI CCWGG 1 cut(s) 316
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 1 cut(s) 193
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 2 cut(s) 89, 143
BseYI CCCAGC 1 cut(s) 278
BsgI GTGCAG 1 cut(s) 142
BshFI GGCC 2 cut(s) 56, 319
BsiHKAI GWGCWC 1 cut(s) 110
BsiSI CCGG 1 cut(s) 95
BsmAI GTCTC 2 cut(s) 191, 380
BsnI GGCC 2 cut(s) 56, 319
Bso31I GGTCTC 1 cut(s) 380
Bsp1286I GDGCHC 2 cut(s) 110, 273
Bsp143I GATC 1 cut(s) 352
Bsp1720I GCTNAGC 1 cut(s) 104
BspANI GGCC 2 cut(s) 56, 319
BspCNI CTCAG 1 cut(s) 96
BspTNI GGTCTC 1 cut(s) 380
BsrI ACTGG 1 cut(s) 193
BssMI GATC 1 cut(s) 352
Bst2UI CCWGG 1 cut(s) 316
Bst4CI ACNGT 1 cut(s) 144
Bst6I CTCTTC 1 cut(s) 57
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 1 cut(s) 104
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 2 cut(s) 191, 380
BstMBI GATC 1 cut(s) 352
BstMWI GCNNNNNNNGC 1 cut(s) 363
BstNI CCWGG 1 cut(s) 316
BstSCI CCNGG 2 cut(s) 93, 314
BstSLI GKGCMC 1 cut(s) 273
BstV1I GCAGC 2 cut(s) 89, 143
BsuRI GGCC 2 cut(s) 56, 319
Cac8I GCNNGC 1 cut(s) 54
Csp6I GTAC 1 cut(s) 196
CviJI RGCY 9 cut(s) 56, 80, 103, 108, 181, 234, 282, 304, 319
CviKI_1 RGCY 9 cut(s) 56, 80, 103, 108, 181, 234, 282, 304, 319
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 104
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 110
Eco31I GGTCTC 1 cut(s) 380
Eco53kI GAGCTC 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 314
EcoT38I GRGCYC 1 cut(s) 110
FaiI YATR 5 cut(s) 13, 31, 154, 311, 372
Fnu4HI GCNGC 2 cut(s) 78, 157
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 2 cut(s) 78, 157
FspBI CTAG 2 cut(s) 255, 305
GluI GCNGC 2 cut(s) 78, 157
GsaI CCCAGC 1 cut(s) 282
HaeIII GGCC 2 cut(s) 56, 319
HapII CCGG 1 cut(s) 95
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 1 cut(s) 392
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 1 cut(s) 95
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 246, 352
Hpy188III TCNNGA 1 cut(s) 396
Hpy8I GTNNAC 1 cut(s) 229
HpyCH4III ACNGT 1 cut(s) 144
HpyCH4V TGCA 5 cut(s) 26, 90, 116, 122, 159
HpyF10VI GCNNNNNNNGC 1 cut(s) 363
HpyF3I CTNAG 1 cut(s) 104
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 113
LpnPI CCDG 7 cut(s) 38, 108, 124, 206, 292, 301, 328
Lsp1109I GCAGC 2 cut(s) 89, 143
LweI GCATC 2 cut(s) 17, 39
MaeI CTAG 2 cut(s) 255, 305
MaeIII GTNAC 1 cut(s) 82
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 1 cut(s) 74
MfeI CAATTG 1 cut(s) 21
MhlI GDGCHC 2 cut(s) 110, 273
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 3 cut(s) 21, 221, 323
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 3 cut(s) 58, 209, 224
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MseI TTAA 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 2 cut(s) 95, 316
MunI CAATTG 1 cut(s) 21
MvaI CCWGG 1 cut(s) 316
MwoI GCNNNNNNNGC 1 cut(s) 363
NciI CCSGG 1 cut(s) 95
NdeII GATC 1 cut(s) 352
NmuCI GTSAC 1 cut(s) 82
PkrI GCNGC 2 cut(s) 79, 158
PleI GAGTC 1 cut(s) 386
PpsI GAGTC 1 cut(s) 386
Psp124BI GAGCTC 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 314
PspFI CCCAGC 1 cut(s) 278
PspGI CCWGG 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SacI GAGCTC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 2 cut(s) 78, 157
Sau3AI GATC 1 cut(s) 352
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 2 cut(s) 95, 316
SduI GDGCHC 2 cut(s) 110, 273
SetI ASST 8 cut(s) 69, 82, 105, 110, 201, 216, 306, 317
SfaNI GCATC 2 cut(s) 17, 39
SmlI CTYRAG 2 cut(s) 215, 394
SmoI CTYRAG 2 cut(s) 215, 394
Sse9I AATT 3 cut(s) 21, 221, 323
SspI AATATT 1 cut(s) 288
SspMI CTAG 2 cut(s) 255, 305
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 2 cut(s) 93, 314
TaaI ACNGT 1 cut(s) 144
TasI AATT 3 cut(s) 21, 221, 323
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 2 cut(s) 77, 156
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 1 cut(s) 17
XspI CTAG 2 cut(s) 255, 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.