MD07G1054500.v1.1

TRAF-type zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004794
Physical Location & Seq
Reverse (-)
5026998 .. 5029863
2866 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1054500.v1.1.491

Sequence Viewer

Length: 1950 bp
ATGATACAGGAATTGTCAACCATTGATGATCTGCCTGCGAGTAATGTGGAACTCAAACCGGAAAAGATTGAAGAAGGGAAGGATGGAGGTCCTTCGTTTCATTGTGATCTTTACGACACAGAATTAGTTTACAAGATAGCTCAAGTGTTCATCCCTGGATTAGCTACAGCTTGTGTTGACAACACATCTGGTGATATCTTCAGGACCGCTGGTTCAGTGGCTGCTGATCTCAGAAAGGAAATGGTGGAGTATATTACTCAGAGAAGCGAAAATTTTGTAGCGGAGTCTGTTATCTTAGAAGGTGGGACGGATGCACAAGTCTCTGATCATCCGTATGACATCATTTCTGATTTTGTTGATGACTTTGCAAGTTCCAAGAGAAATCTGTTTAGTCGGGTTACAGGATGGCTGCTAAGTGAAAAAAGAGAAGATAACATAGATGATTTCGTTCAAGAGATGGAAATAAACGGGTTTTGGTTGATTGATAAAAGAGAAGCAATAGCACAGACTCTGCTTAAGAATGTCGACTTTAAGAATGAACATCACTGCAACATGAAGTTTAACTCTCAAGAAGCGGTTGCCGAGCATGCTTCAAGCTGCAGTTTTAGGTCTATGACTTGCACAAATGAAGGGTGTACTATTGTATTTTGTGCAAGTCACATGGAGAAACACGATTCCGTTTGCCCGTTCAAGATAATTCCATGTGAACAAAACTGCTCCAGTACCATCATGAGACGCGAGATGGACAGACATTGTATTACCGTCTGTCCAATGAAGCTTGTTAGTTGTCCTTTCTATGCAGTGGGTTGTCAATCTCCCGTACCACACTGTAAAGTTGAGCAACATCGTTCGGATGATCGCCATTCTCACTTGCTTTTCATTCTTCAAACTATTCACAAGGAAGCATCTGCAGAGGATCTAAAGAAACGGGTGGAGCAAATAGAAAAGGCATCTTCATCGAGTCAATTAGCTGATGCTCGAGATGTGAGATCGTTAACATCTGCAGTCAAGGGTATTGAAGAGAAACTTGGTCCAATGGAGATGAGCAGCAAACACCCAGAGGATGTTGAAGCAAAGCCAGGACCAGCAGAATTGAACGGCGAACATCCAGAGGATGTTGAAGCAAAGTTAGGGCCAACGGAAGTGACCAGCAAACCCACCGAGGATCTTGAAGCAAGACTTCAGCCGCACGAAGTGAACAAAAGAACCCCAGAGGTTGTTGAAACAAAACTAGGGCCAAAGGAAGTGAGCAGCAAACCCCAAGAGGATCCTCAAACTAAACTAGGGTCAAAAGAAATGAGCAGCAATACCTCCAAGGATCTTGAAGCAAACCAAGGGTCAAAGGAAGTGAGCAGCAAAACCTCTGAGGATCCTGAAGCAAAGCTTGGGACAAAGGAACCGAGCAGAAAAGTTGAAAAAAAACAAGCGCCAAAGGAAGTGGACAGCAAACCCCCAGAGGATCATGAAGCAAAAATTGGGACAAATGAAGTGAGCAGCCAACCCCGAGAGGACCATGAAACAAGCCAAGGGTCAAAGGAAGTGAGCAGTAAACCCCCAGAGGATCCTGAAGTAAAGCTTGGGTCAAAGGAACTGAGCAGAAAATCCCCGGAGGATGTTGAAAAAAAACTTGCGCCAAAGGAATTGGACAGCACACTACCAGAGGATCACGAACCAAAAACCAGGACAAAGGATGTGGACAATGAATCCCCGAAGGATATTGAAGCAAAACGTGGGCCAAAGGAAGTGAACAACGAATCCCCGAAGGATATTGAAGCAAAAGTAGGGCAGGATTTTGAAGCAAAAGTAACTCCTAGTGAAAGCAGCAGCAAACCACCAGAGGATCTTGAAGCAAAACTGGGGCCAAAAGAAGCGAGCGGCAAACACAAGGATAGTGAAGAGAGTAGAGAAACCTCAACTTCGAAAAATGTCTTGGAGGAAGCTCAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 map00130
Pfam Domains
Protein Families
zf-TRAF

Protein Analysis

650

Amino Acids

72.05

Weight (kDa)

5.02

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-TRAF PF02176 224 - 282 8.4e-11 TRAF-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1875
AccI GTMKAC 1 cut(s) 525
AccII CGCG 1 cut(s) 738
AciI CCGC 5 cut(s) 207, 281, 575, 1187, 1875
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 4 cut(s) 184, 1166, 1395, 1587
AdeI CACNNNGTG 1 cut(s) 1195
AfaI GTAC 3 cut(s) 637, 724, 822
AflII CTTAAG 1 cut(s) 515
AjnI CCWGG 3 cut(s) 154, 1078, 1679
AloI GAACNNNNNNTCC 4 cut(s) 196, 228, 1739, 1771
AluBI AGCT 9 cut(s) 140, 164, 170, 597, 778, 971, 1384, 1576, 1940
AluI AGCT 9 cut(s) 140, 164, 170, 597, 778, 971, 1384, 1576, 1940
Alw26I GTCTC 2 cut(s) 325, 727
AlwNI CAGNNNCTG 2 cut(s) 221, 511
Ama87I CYCGRG 2 cut(s) 978, 1503
AoxI GGCC 4 cut(s) 1133, 1235, 1733, 1859
ApoI RAATTY 1 cut(s) 271
ArsI GACNNNNNNTTYG 2 cut(s) 1322, 1354
AspLEI GCGC 2 cut(s) 1429, 1633
AspS9I GGNCC 9 cut(s) 89, 204, 1031, 1082, 1133, 1235, 1510, 1733, 1859
AsuC2I CCSGG 1 cut(s) 1607
AsuHPI GGTGA 1 cut(s) 203
AsuII TTCGAA 1 cut(s) 1919
AvaI CYCGRG 2 cut(s) 978, 1503
AvaII GGWCC 5 cut(s) 89, 204, 1031, 1082, 1510
BamHI GGATCC 3 cut(s) 1267, 1369, 1561
BccI CCATC 5 cut(s) 77, 399, 451, 734, 736
BceAI ACGGC 1 cut(s) 1114
BcgI CGANNNNNNTGC 2 cut(s) 939, 973
BciT130I CCWGG 3 cut(s) 156, 1080, 1681
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 1 cut(s) 1607
BcoDI GTCTC 2 cut(s) 325, 727
BfaI CTAG 3 cut(s) 1232, 1283, 1812
BfmI CTRYAG 4 cut(s) 165, 598, 909, 1002
BfoI RGCGCY 1 cut(s) 1430
BfrI CTTAAG 1 cut(s) 515
Bme1390I CCNGG 4 cut(s) 156, 1080, 1607, 1681
Bme18I GGWCC 5 cut(s) 89, 204, 1031, 1082, 1510
BmeT110I CYCGRG 2 cut(s) 978, 1503
BmgT120I GGNCC 9 cut(s) 89, 204, 1031, 1082, 1133, 1235, 1510, 1733, 1859
BmiI GGNNCC 5 cut(s) 1269, 1371, 1398, 1563, 1860
BmrFI CCNGG 4 cut(s) 156, 1080, 1607, 1681
BmrI ACTGGG 1 cut(s) 1865
BmsI GCATC 4 cut(s) 301, 914, 959, 964
BmuI ACTGGG 1 cut(s) 1865
BpmI CTGGAG 1 cut(s) 703
Bpu14I TTCGAA 1 cut(s) 1919
BpuEI CTTGAG 2 cut(s) 126, 552
BpuMI CCSGG 1 cut(s) 1607
BsaJI CCNNGG 6 cut(s) 154, 1161, 1314, 1333, 1525, 1605
BsaWI WCCGGW 1 cut(s) 58
Bse1I ACTGG 2 cut(s) 720, 1860
BseBI CCWGG 3 cut(s) 156, 1080, 1681
BseDI CCNNGG 6 cut(s) 154, 1161, 1314, 1333, 1525, 1605
BseMII CTCAG 4 cut(s) 244, 272, 1356, 1583
BseNI ACTGG 2 cut(s) 720, 1860
Bsh1236I CGCG 1 cut(s) 738
BshFI GGCC 4 cut(s) 1135, 1237, 1735, 1861
BsiHKCI CYCGRG 2 cut(s) 978, 1503
BsiSI CCGG 2 cut(s) 59, 1607
BslFI GGGAC 3 cut(s) 319, 1402, 1492
BsmAI GTCTC 2 cut(s) 325, 727
BsmBI CGTCTC 1 cut(s) 727
BsmFI GGGAC 3 cut(s) 319, 1402, 1492
BsnI GGCC 4 cut(s) 1135, 1237, 1735, 1861
BsoBI CYCGRG 2 cut(s) 978, 1503
Bsp119I TTCGAA 1 cut(s) 1919
BspACI CCGC 5 cut(s) 207, 281, 575, 1187, 1875
BspANI GGCC 4 cut(s) 1135, 1237, 1735, 1861
BspCNI CTCAG 4 cut(s) 243, 271, 1357, 1584
BspFNI CGCG 1 cut(s) 738
BspHI TCATGA 2 cut(s) 729, 1462
BspLI GGNNCC 5 cut(s) 1269, 1371, 1398, 1563, 1860
BspMAI CTGCAG 3 cut(s) 602, 913, 1006
BspT104I TTCGAA 1 cut(s) 1919
BspTI CTTAAG 1 cut(s) 515
BsrBI CCGCTC 1 cut(s) 1875
BsrI ACTGG 2 cut(s) 720, 1860
BssECI CCNNGG 6 cut(s) 154, 1161, 1314, 1333, 1525, 1605
BssT1I CCWWGG 3 cut(s) 1314, 1333, 1525
Bst2UI CCWGG 3 cut(s) 156, 1080, 1681
Bst4CI ACNGT 2 cut(s) 763, 830
Bst6I CTCTTC 2 cut(s) 1014, 1890
BstAFI CTTAAG 1 cut(s) 515
BstBI TTCGAA 1 cut(s) 1919
BstC8I GCNNGC 3 cut(s) 36, 588, 1873
BstDEI CTNAG 6 cut(s) 230, 258, 295, 413, 1365, 1592
BstFNI CGCG 1 cut(s) 738
BstH2I RGCGCY 1 cut(s) 1430
BstHHI GCGC 2 cut(s) 1429, 1633
BstMAI GTCTC 2 cut(s) 325, 727
BstMWI GCNNNNNNNGC 1 cut(s) 587
BstNI CCWGG 3 cut(s) 156, 1080, 1681
BstNSI RCATGY 1 cut(s) 590
BstSCI CCNGG 4 cut(s) 154, 1078, 1605, 1679
BstSFI CTRYAG 4 cut(s) 165, 598, 909, 1002
BstUI CGCG 1 cut(s) 738
BstX2I RGATCY 7 cut(s) 916, 1165, 1267, 1318, 1369, 1561, 1840
BstYI RGATCY 7 cut(s) 916, 1165, 1267, 1318, 1369, 1561, 1840
BsuRI GGCC 4 cut(s) 1135, 1237, 1735, 1861
BtsI GCAGTG 2 cut(s) 544, 807
BtsIMutI CAGTG 4 cut(s) 222, 544, 807, 826
Cac8I GCNNGC 3 cut(s) 36, 588, 1873
CaiI CAGNNNCTG 2 cut(s) 221, 511
CciI TCATGA 2 cut(s) 729, 1462
CfoI GCGC 2 cut(s) 1429, 1633
Cfr13I GGNCC 9 cut(s) 89, 204, 1031, 1082, 1133, 1235, 1510, 1733, 1859
CseI GACGC 1 cut(s) 744
Csp6I GTAC 3 cut(s) 636, 723, 821
CspCI CAANNNNNGTGG 4 cut(s) 1419, 1454, 1674, 1709
CviAII CATG 8 cut(s) 553, 587, 661, 702, 730, 1463, 1514, 1947
CviQI GTAC 3 cut(s) 636, 723, 821
DdeI CTNAG 6 cut(s) 230, 258, 295, 413, 1365, 1592
DraIII CACNNNGTG 1 cut(s) 1195
Eam1104I CTCTTC 2 cut(s) 1014, 1890
EarI CTCTTC 2 cut(s) 1014, 1890
Eco130I CCWWGG 3 cut(s) 1314, 1333, 1525
Eco32I GATATC 1 cut(s) 196
Eco47I GGWCC 5 cut(s) 89, 204, 1031, 1082, 1510
Eco57I CTGAAG 4 cut(s) 184, 1166, 1395, 1587
Eco88I CYCGRG 2 cut(s) 978, 1503
EcoO109I RGGNCCY 1 cut(s) 89
EcoRII CCWGG 3 cut(s) 154, 1078, 1679
EcoRV GATATC 1 cut(s) 196
EcoT14I CCWWGG 3 cut(s) 1314, 1333, 1525
ErhI CCWWGG 3 cut(s) 1314, 1333, 1525
Esp3I CGTCTC 1 cut(s) 727
FaeI CATG 8 cut(s) 556, 590, 664, 705, 733, 1466, 1517, 1950
FalI AAGNNNNNCTT 8 cut(s) 1011, 1043, 1164, 1196, 1368, 1400, 1560, 1592
FaqI GGGAC 3 cut(s) 319, 1402, 1492
FatI CATG 8 cut(s) 552, 586, 660, 701, 729, 1462, 1513, 1946
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 1 cut(s) 525
FspBI CTAG 3 cut(s) 1232, 1283, 1812
GlaI GCGC 2 cut(s) 1428, 1632
GsuI CTGGAG 1 cut(s) 703
HaeII RGCGCY 1 cut(s) 1430
HaeIII GGCC 4 cut(s) 1135, 1237, 1735, 1861
HapII CCGG 2 cut(s) 59, 1607
HgaI GACGC 1 cut(s) 744
HhaI GCGC 2 cut(s) 1429, 1633
Hin1II CATG 8 cut(s) 556, 590, 664, 705, 733, 1466, 1517, 1950
Hin6I GCGC 2 cut(s) 1427, 1631
HinP1I GCGC 2 cut(s) 1427, 1631
HincII GTYRAC 4 cut(s) 18, 178, 526, 996
HindII GTYRAC 4 cut(s) 18, 178, 526, 996
HindIII AAGCTT 3 cut(s) 776, 1382, 1574
HinfI GANTC 6 cut(s) 284, 508, 674, 961, 1703, 1754
HpaI GTTAAC 1 cut(s) 996
HpaII CCGG 2 cut(s) 59, 1607
HphI GGTGA 1 cut(s) 203
Hpy188I TCNGA 6 cut(s) 233, 261, 325, 349, 853, 1366
HpyAV CCTTC 7 cut(s) 68, 73, 102, 293, 623, 1705, 1756
HpyCH4III ACNGT 2 cut(s) 763, 830
HpyCH4IV ACGT 1 cut(s) 1729
HpyCH4V TGCA 9 cut(s) 314, 368, 549, 600, 621, 653, 800, 911, 1004
HpyF10VI GCNNNNNNNGC 1 cut(s) 587
HpyF3I CTNAG 6 cut(s) 230, 258, 295, 413, 1365, 1592
HpySE526I ACGT 1 cut(s) 1729
Hsp92II CATG 8 cut(s) 556, 590, 664, 705, 733, 1466, 1517, 1950
HspAI GCGC 2 cut(s) 1427, 1631
Ksp22I TGATCA 1 cut(s) 325
KspAI GTTAAC 1 cut(s) 996
LmnI GCTCC 2 cut(s) 722, 934
LweI GCATC 4 cut(s) 301, 914, 959, 964
MaeI CTAG 3 cut(s) 1232, 1283, 1812
MaeII ACGT 1 cut(s) 1729
MaeIII GTNAC 4 cut(s) 397, 656, 1144, 1804
MbiI CCGCTC 1 cut(s) 1875
MboII GAAGA 7 cut(s) 83, 190, 440, 875, 945, 1031, 1907
MflI RGATCY 7 cut(s) 916, 1165, 1267, 1318, 1369, 1561, 1840
MluCI AATT 8 cut(s) 11, 122, 271, 696, 965, 1091, 1473, 1640
MlyI GAGTC 3 cut(s) 293, 502, 970
MseI TTAA 4 cut(s) 516, 531, 561, 995
MslI CAYNNNNRTG 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 209
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 2 cut(s) 59, 1607
MspR9I CCNGG 4 cut(s) 156, 1080, 1607, 1681
MvaI CCWGG 3 cut(s) 156, 1080, 1681
MvnI CGCG 1 cut(s) 738
MwoI GCNNNNNNNGC 1 cut(s) 587
NciI CCSGG 1 cut(s) 1607
NlaIII CATG 8 cut(s) 556, 590, 664, 705, 733, 1466, 1517, 1950
NlaIV GGNNCC 5 cut(s) 1269, 1371, 1398, 1563, 1860
NmeAIII GCCGAG 1 cut(s) 607
NmuCI GTSAC 2 cut(s) 656, 1144
NspI RCATGY 1 cut(s) 590
NspV TTCGAA 1 cut(s) 1919
PaeI GCATGC 1 cut(s) 590
PaeR7I CTCGAG 1 cut(s) 978
PagI TCATGA 2 cut(s) 729, 1462
PfeI GAWTC 3 cut(s) 674, 1703, 1754
PleI GAGTC 3 cut(s) 292, 502, 969
PpsI GAGTC 3 cut(s) 292, 502, 969
PpuMI RGGWCCY 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 89
Psp6I CCWGG 3 cut(s) 154, 1078, 1679
PspGI CCWGG 3 cut(s) 154, 1078, 1679
PspN4I GGNNCC 5 cut(s) 1269, 1371, 1398, 1563, 1860
PspPI GGNCC 9 cut(s) 89, 204, 1031, 1082, 1133, 1235, 1510, 1733, 1859
PspPPI RGGWCCY 1 cut(s) 89
PstI CTGCAG 3 cut(s) 602, 913, 1006
PstNI CAGNNNCTG 2 cut(s) 221, 511
PsuI RGATCY 7 cut(s) 916, 1165, 1267, 1318, 1369, 1561, 1840
RsaI GTAC 3 cut(s) 637, 724, 822
RsaNI GTAC 3 cut(s) 636, 723, 821
RseI CAYNNNNRTG 1 cut(s) 333
SalI GTCGAC 1 cut(s) 524
SaqAI TTAA 4 cut(s) 516, 531, 561, 995
Sau96I GGNCC 9 cut(s) 89, 204, 1031, 1082, 1133, 1235, 1510, 1733, 1859
SchI GAGTC 3 cut(s) 293, 502, 970
ScrFI CCNGG 4 cut(s) 156, 1080, 1607, 1681
SfaNI GCATC 4 cut(s) 301, 914, 959, 964
SfcI CTRYAG 4 cut(s) 165, 598, 909, 1002
Sfr274I CTCGAG 1 cut(s) 978
SfuI TTCGAA 1 cut(s) 1919
SinI GGWCC 5 cut(s) 89, 204, 1031, 1082, 1510
SlaI CTCGAG 1 cut(s) 978
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 4 cut(s) 141, 515, 567, 978
SmoI CTYRAG 4 cut(s) 141, 515, 567, 978
SphI GCATGC 1 cut(s) 590
Sse9I AATT 8 cut(s) 11, 122, 271, 696, 965, 1091, 1473, 1640
SsiI CCGC 5 cut(s) 207, 281, 575, 1187, 1875
SspMI CTAG 3 cut(s) 1232, 1283, 1812
StyD4I CCNGG 4 cut(s) 154, 1078, 1605, 1679
StyI CCWWGG 3 cut(s) 1314, 1333, 1525
TaaI ACNGT 2 cut(s) 763, 830
TaiI ACGT 1 cut(s) 1732
TaqI TCGA 4 cut(s) 525, 959, 979, 1919
TasI AATT 8 cut(s) 11, 122, 271, 696, 965, 1091, 1473, 1640
TatI WGTACW 1 cut(s) 635
TauI GCSGC 2 cut(s) 1189, 1878
TfiI GAWTC 3 cut(s) 674, 1703, 1754
Tru1I TTAA 4 cut(s) 516, 531, 561, 995
Tru9I TTAA 4 cut(s) 516, 531, 561, 995
TscAI CASTG 4 cut(s) 222, 551, 807, 833
TseFI GTSAC 2 cut(s) 656, 1144
Tsp45I GTSAC 2 cut(s) 656, 1144
TspGWI ACGGA 4 cut(s) 321, 323, 667, 1154
TspRI CASTG 4 cut(s) 222, 551, 807, 833
Vha464I CTTAAG 1 cut(s) 515
VpaK11BI GGWCC 5 cut(s) 89, 204, 1031, 1082, 1510
XapI RAATTY 1 cut(s) 271
XceI RCATGY 1 cut(s) 590
XhoI CTCGAG 1 cut(s) 978
XmiI GTMKAC 1 cut(s) 525
XspI CTAG 3 cut(s) 1232, 1283, 1812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.