MD07G1295200.v1.1

Dof zinc finger protein DOF4.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002079
Physical Location & Seq
Forward (+)
35586892 .. 35589011
2120 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1295200.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGAAGATTTTTCTTCTACAAGGCCAATCACAGAGAGAAGGGCAAGGCCACAAAAGGATCAAGCTTTGAATTGTCCAAGGTGTAATTCCACCACCACAAAATTCTGTTATTACAACAATTACAGCCTCTCTCAGCCAAGATACTTTTGCAAGACTTGTAAAAGGTATTGGACTGAAGGTGGATCTCTGAGAAATGTTCCTGTGGGTGGAGGCTCGCGGAAGAACAACAAGAGATCAAATAATTCCGATTCATCGTCACCGTCCACGTCTTCAGCGAAGAAGGTTGCTTATGATCATGATCACCATCTGACCCCACATAGCAATTTTCCTCCTCAGTCTGCTTCTCAAAACCCTAATAAGATCCACTTCCAAGGCCAAGATCTCAACCTTGCATACCCAACAGCTGATCAGGACTATGACAACAACAATATTACCAAACTATCCTTTAGTATTGACAACAAAACCTACCACCCAAACCCTAGCTCTTCGGGCACTACTACTACATCTCATCATTTCTCATCGGCTATGGAACTGCTCACAAGTACCGGGATTGCATCAAGGAGTCTGACTTCTTTCGTACCGATGGCAGCAACGGCCGTGCCGGACCATTCGAATAATATCACAATGTTTTCTGCTGGGTTTCCTTTGCAAGAGTTCAAGCCAAGTGGGCTTAGTTTTTCTCTAGATGGGTTTGAGAGTGCTGGTTATGGTGGGTGCCTACAAGGTGTGCAAGAGAATACTACTAGTCATGCCAGGCTTTTGTTTCCTACTGATCAGGATTTTAAGCAGCAGATTCCGAGCTCCACTACTACTGCTGAATTTGATCAGCACAATAATAGAGGAGGGGAAGGAGATTCTGGTGGTGGGTATTGGAATGGAATGTTAGGTGGGGGATCATGGTAA

Protein Analysis

301

Amino Acids

32.89

Weight (kDa)

7.68

Isoelectric Point (pI)

55.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 20 - 77 7.5e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 714
AccII CGCG 1 cut(s) 217
AciI CCGC 1 cut(s) 217
AclWI GGATC 4 cut(s) 66, 190, 355, 901
AcoI YGGCCR 1 cut(s) 594
AcsI RAATTY 2 cut(s) 101, 818
AcuI CTGAAG 2 cut(s) 195, 255
AfaI GTAC 2 cut(s) 544, 579
AfiI CCNNNNNNNGG 1 cut(s) 206
AgsI TTSAA 2 cut(s) 70, 658
AhlI ACTAGT 1 cut(s) 743
AjiI CACGTC 1 cut(s) 267
AjnI CCWGG 1 cut(s) 752
AjuI GAANNNNNNNTTGG 2 cut(s) 70, 102
AluBI AGCT 4 cut(s) 65, 404, 483, 801
AluI AGCT 4 cut(s) 65, 404, 483, 801
Alw21I GWGCWC 1 cut(s) 803
AlwI GGATC 4 cut(s) 66, 190, 355, 901
AoxI GGCC 4 cut(s) 23, 47, 373, 594
ApeKI GCWGC 2 cut(s) 587, 787
ApoI RAATTY 2 cut(s) 101, 818
AspS9I GGNCC 1 cut(s) 604
AsuC2I CCSGG 1 cut(s) 547
AsuHPI GGTGA 2 cut(s) 249, 293
AsuII TTCGAA 1 cut(s) 611
AvaII GGWCC 1 cut(s) 604
BaeGI GKGCMC 1 cut(s) 494
BanI GGYRCC 1 cut(s) 714
BanII GRGCYC 1 cut(s) 803
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 803
BbvI GCAGC 2 cut(s) 599, 799
BccI CCATC 3 cut(s) 312, 577, 680
BceAI ACGGC 2 cut(s) 581, 609
BciT130I CCWGG 1 cut(s) 754
BclI TGATCA 5 cut(s) 292, 298, 406, 772, 823
BcnI CCSGG 1 cut(s) 547
BcuI ACTAGT 1 cut(s) 743
BfaI CTAG 3 cut(s) 480, 683, 744
BglI GCCNNNNNGGC 1 cut(s) 667
BglII AGATCT 1 cut(s) 379
BisI GCNGC 2 cut(s) 588, 788
BlsI GCNGC 2 cut(s) 589, 789
Bme1390I CCNGG 2 cut(s) 547, 754
Bme18I GGWCC 1 cut(s) 604
BmgBI CACGTC 1 cut(s) 267
BmgT120I GGNCC 1 cut(s) 604
BmiI GGNNCC 1 cut(s) 716
BmrFI CCNGG 2 cut(s) 547, 754
BmsI GCATC 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 261
Bpu14I TTCGAA 1 cut(s) 611
BpuMI CCSGG 1 cut(s) 547
BsaBI GATNNNNATC 2 cut(s) 297, 303
BsaJI CCNNGG 2 cut(s) 77, 370
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse8I GATNNNNATC 2 cut(s) 297, 303
BseBI CCWGG 1 cut(s) 754
BseDI CCNNGG 2 cut(s) 77, 370
BseJI GATNNNNATC 2 cut(s) 297, 303
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMII CTCAG 3 cut(s) 146, 179, 347
BseRI GAGGAG 2 cut(s) 321, 855
BseSI GKGCMC 1 cut(s) 494
BseX3I CGGCCG 1 cut(s) 594
BseXI GCAGC 2 cut(s) 599, 799
BseYI CCCAGC 1 cut(s) 635
Bsh1236I CGCG 1 cut(s) 217
Bsh1285I CGRYCG 1 cut(s) 597
BshFI GGCC 4 cut(s) 25, 49, 375, 596
BshNI GGYRCC 1 cut(s) 714
BsiEI CGRYCG 1 cut(s) 597
BsiHKAI GWGCWC 1 cut(s) 803
BsiSI CCGG 2 cut(s) 546, 602
BslI CCNNNNNNNGG 1 cut(s) 206
BsnI GGCC 4 cut(s) 25, 49, 375, 596
Bsp119I TTCGAA 1 cut(s) 611
Bsp1286I GDGCHC 2 cut(s) 494, 803
BspACI CCGC 1 cut(s) 217
BspANI GGCC 4 cut(s) 25, 49, 375, 596
BspCNI CTCAG 3 cut(s) 145, 180, 346
BspFNI CGCG 1 cut(s) 217
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 716
BspPI GGATC 4 cut(s) 66, 190, 355, 901
BspQI GCTCTTC 1 cut(s) 490
BspT104I TTCGAA 1 cut(s) 611
BspT107I GGYRCC 1 cut(s) 714
BssECI CCNNGG 2 cut(s) 77, 370
BssT1I CCWWGG 2 cut(s) 77, 370
Bst2UI CCWGG 1 cut(s) 754
Bst4CI ACNGT 1 cut(s) 261
Bst6I CTCTTC 1 cut(s) 490
BstBI TTCGAA 1 cut(s) 611
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 4 cut(s) 132, 188, 333, 671
BstFNI CGCG 1 cut(s) 217
BstMCI CGRYCG 1 cut(s) 597
BstMWI GCNNNNNNNGC 3 cut(s) 489, 593, 667
BstNI CCWGG 1 cut(s) 754
BstSCI CCNGG 2 cut(s) 545, 752
BstSLI GKGCMC 1 cut(s) 494
BstUI CGCG 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 599, 799
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 3 cut(s) 182, 360, 379
BstYI RGATCY 3 cut(s) 182, 360, 379
BstZI CGGCCG 1 cut(s) 594
BsuRI GGCC 4 cut(s) 25, 49, 375, 596
BtrI CACGTC 1 cut(s) 267
Cac8I GCNNGC 1 cut(s) 215
CciI TCATGA 1 cut(s) 295
Cfr13I GGNCC 1 cut(s) 604
Csp6I GTAC 2 cut(s) 543, 578
CspCI CAANNNNNGTGG 2 cut(s) 646, 681
CviAII CATG 3 cut(s) 296, 749, 897
CviQI GTAC 2 cut(s) 543, 578
DdeI CTNAG 4 cut(s) 132, 188, 333, 671
EaeI YGGCCR 1 cut(s) 594
EagI CGGCCG 1 cut(s) 594
Eam1104I CTCTTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 490
Ecl136II GAGCTC 1 cut(s) 801
EclXI CGGCCG 1 cut(s) 594
Eco130I CCWWGG 2 cut(s) 77, 370
Eco24I GRGCYC 1 cut(s) 803
Eco47I GGWCC 1 cut(s) 604
Eco52I CGGCCG 1 cut(s) 594
Eco53kI GAGCTC 1 cut(s) 801
Eco57I CTGAAG 2 cut(s) 195, 255
EcoICRI GAGCTC 1 cut(s) 801
EcoRII CCWGG 1 cut(s) 752
EcoT14I CCWWGG 2 cut(s) 77, 370
EcoT38I GRGCYC 1 cut(s) 803
ErhI CCWWGG 2 cut(s) 77, 370
FaeI CATG 3 cut(s) 299, 752, 900
FaiI YATR 9 cut(s) 291, 297, 318, 394, 417, 527, 708, 750, 898
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FatI CATG 3 cut(s) 295, 748, 896
FbaI TGATCA 5 cut(s) 292, 298, 406, 772, 823
Fnu4HI GCNGC 2 cut(s) 588, 788
FriOI GRGCYC 1 cut(s) 803
Fsp4HI GCNGC 2 cut(s) 588, 788
FspBI CTAG 3 cut(s) 480, 683, 744
GluI GCNGC 2 cut(s) 588, 788
GsaI CCCAGC 1 cut(s) 639
HaeIII GGCC 4 cut(s) 25, 49, 375, 596
HapII CCGG 2 cut(s) 546, 602
Hin1II CATG 3 cut(s) 299, 752, 900
HindIII AAGCTT 1 cut(s) 63
HinfI GANTC 4 cut(s) 248, 562, 793, 854
HpaII CCGG 2 cut(s) 546, 602
HphI GGTGA 2 cut(s) 249, 293
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 5 cut(s) 189, 247, 309, 567, 798
Hpy188III TCNNGA 4 cut(s) 296, 410, 683, 776
Hpy8I GTNNAC 1 cut(s) 264
HpyAV CCTTC 4 cut(s) 33, 170, 274, 842
HpyCH4III ACNGT 1 cut(s) 261
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 5 cut(s) 150, 392, 554, 649, 730
HpyF10VI GCNNNNNNNGC 3 cut(s) 489, 593, 667
HpyF3I CTNAG 4 cut(s) 132, 188, 333, 671
HpySE526I ACGT 1 cut(s) 266
Hsp92II CATG 3 cut(s) 299, 752, 900
Ksp22I TGATCA 5 cut(s) 292, 298, 406, 772, 823
LguI GCTCTTC 1 cut(s) 490
LmnI GCTCC 1 cut(s) 806
Lsp1109I GCAGC 2 cut(s) 599, 799
LweI GCATC 1 cut(s) 563
MaeI CTAG 3 cut(s) 480, 683, 744
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 1 cut(s) 255
MboII GAAGA 6 cut(s) 6, 17, 232, 261, 289, 477
MflI RGATCY 3 cut(s) 182, 360, 379
MhlI GDGCHC 2 cut(s) 494, 803
MluCI AATT 7 cut(s) 70, 85, 101, 118, 241, 322, 818
MlyI GAGTC 1 cut(s) 571
MnlI CCTC 6 cut(s) 137, 203, 339, 342, 833, 836
MseI TTAA 1 cut(s) 783
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 2 cut(s) 546, 602
MspR9I CCNGG 2 cut(s) 547, 754
MvaI CCWGG 1 cut(s) 754
MvnI CGCG 1 cut(s) 217
MwoI GCNNNNNNNGC 3 cut(s) 489, 593, 667
NciI CCSGG 1 cut(s) 547
NlaIII CATG 3 cut(s) 299, 752, 900
NlaIV GGNNCC 1 cut(s) 716
NmuCI GTSAC 1 cut(s) 255
NspV TTCGAA 1 cut(s) 611
PagI TCATGA 1 cut(s) 295
PciSI GCTCTTC 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 272
PfeI GAWTC 3 cut(s) 248, 793, 854
PkrI GCNGC 2 cut(s) 589, 789
PleI GAGTC 1 cut(s) 570
PpsI GAGTC 1 cut(s) 570
Psp124BI GAGCTC 1 cut(s) 803
Psp6I CCWGG 1 cut(s) 752
PspFI CCCAGC 1 cut(s) 635
PspGI CCWGG 1 cut(s) 752
PspN4I GGNNCC 1 cut(s) 716
PspPI GGNCC 1 cut(s) 604
PsuI RGATCY 3 cut(s) 182, 360, 379
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 2 cut(s) 544, 579
RsaNI GTAC 2 cut(s) 543, 578
SacI GAGCTC 1 cut(s) 803
SapI GCTCTTC 1 cut(s) 490
SaqAI TTAA 1 cut(s) 783
SatI GCNGC 2 cut(s) 588, 788
Sau96I GGNCC 1 cut(s) 604
SchI GAGTC 1 cut(s) 571
ScrFI CCNGG 2 cut(s) 547, 754
SduI GDGCHC 2 cut(s) 494, 803
SfaNI GCATC 1 cut(s) 563
SfuI TTCGAA 1 cut(s) 611
SinI GGWCC 1 cut(s) 604
SpeI ACTAGT 1 cut(s) 743
Sse9I AATT 7 cut(s) 70, 85, 101, 118, 241, 322, 818
SsiI CCGC 1 cut(s) 217
SspI AATATT 1 cut(s) 430
SspMI CTAG 3 cut(s) 480, 683, 744
SstI GAGCTC 1 cut(s) 803
StyD4I CCNGG 2 cut(s) 545, 752
StyI CCWWGG 2 cut(s) 77, 370
TaaI ACNGT 1 cut(s) 261
TaiI ACGT 1 cut(s) 269
TaqI TCGA 1 cut(s) 611
TasI AATT 7 cut(s) 70, 85, 101, 118, 241, 322, 818
TfiI GAWTC 3 cut(s) 248, 793, 854
Tru1I TTAA 1 cut(s) 783
Tru9I TTAA 1 cut(s) 783
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 2 cut(s) 587, 787
Tsp45I GTSAC 1 cut(s) 255
TspDTI ATGAA 1 cut(s) 240
VpaK11BI GGWCC 1 cut(s) 604
XapI RAATTY 2 cut(s) 101, 818
XbaI TCTAGA 1 cut(s) 682
XspI CTAG 3 cut(s) 480, 683, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.