MD08G1024100.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002741
Physical Location & Seq
Reverse (-)
1733961 .. 1736792
2832 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1024100.v1.1.491

Sequence Viewer

Length: 486 bp
ATGACTAGCTCCTCCGCCACTTCACGCAAGGCTTTAAGCAAGATCGCCTGCAATCGGCTGCAGAAAGAGCTGGTGGAGTGGCAGGTGAATCCTCCGGCAGGTTTTAGTCACAAAGTCACTGATAATCTTCAGAGATGGATAATCGAAGTCACCGGAGCGCCGGGAACTCTGTACGCTAACGAATTGTATCAGCTCCAAGTTGATTTCCCCGAGCATTACCCCATGGAGGCACCACAGGTGATTTTTGTCTCTCCGGCTCCTCTGCATCCCCACATTTATAGCAATGGCCATATTTGTTTAGACATTCTATATGATTCATGGTCCCCAGCCATGACGGTTAGTTCAATCTGTATCAGCATTCTGTCCATGTTATCGAGTTCCACGGAGAAGCAACGCCCCGCGGATAACGATCGATACGTGAAGAAATGCAGGAACGGCAGATCTCCCAAGGAGACGAGATGGTGGTTCCATGATGACAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

162

Amino Acids

18.35

Weight (kDa)

7.72

Isoelectric Point (pI)

68.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 19 - 154 1.8e-39 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 73
Acc36I ACCTGC 2 cut(s) 73, 89
AccB1I GGYRCC 1 cut(s) 229
AccII CGCG 1 cut(s) 401
AciI CCGC 3 cut(s) 15, 399, 401
AcoI YGGCCR 1 cut(s) 286
AcuI CTGAAG 1 cut(s) 113
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 3 cut(s) 54, 98, 226
AgsI TTSAA 1 cut(s) 345
AluBI AGCT 3 cut(s) 9, 70, 193
AluI AGCT 3 cut(s) 9, 70, 193
Alw26I GTCTC 2 cut(s) 253, 446
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 1 cut(s) 286
ApeKI GCWGC 1 cut(s) 58
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 321
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 3 cut(s) 97, 142, 250
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 321
BalI TGGCCA 1 cut(s) 288
BanI GGYRCC 1 cut(s) 229
BbvI GCAGC 1 cut(s) 45
BccI CCATC 2 cut(s) 129, 453
BceAI ACGGC 1 cut(s) 451
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 2 cut(s) 253, 446
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 59
BfoI RGCGCY 1 cut(s) 161
BfuAI ACCTGC 2 cut(s) 73, 89
BglII AGATCT 1 cut(s) 440
BisI GCNGC 1 cut(s) 59
BlsI GCNGC 1 cut(s) 60
Bme1390I CCNGG 1 cut(s) 162
Bme18I GGWCC 1 cut(s) 321
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 321
BmiI GGNNCC 4 cut(s) 231, 258, 323, 467
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 1 cut(s) 274
BpuMI CCSGG 1 cut(s) 162
Bsa29I ATCGAT 1 cut(s) 412
BsaAI YACGTR 1 cut(s) 418
BsaBI GATNNNNATC 1 cut(s) 408
BsaJI CCNNGG 4 cut(s) 222, 381, 399, 447
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 98, 226
Bse3DI GCAATG 1 cut(s) 289
Bse8I GATNNNNATC 1 cut(s) 408
BseCI ATCGAT 1 cut(s) 412
BseDI CCNNGG 4 cut(s) 222, 381, 399, 447
BseGI GGATG 1 cut(s) 265
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 3 cut(s) 54, 98, 226
BseMI GCAATG 1 cut(s) 289
BseRI GAGGAG 1 cut(s) 249
BseXI GCAGC 1 cut(s) 45
BseYI CCCAGC 1 cut(s) 325
Bsh1236I CGCG 1 cut(s) 401
Bsh1285I CGRYCG 1 cut(s) 412
BshFI GGCC 1 cut(s) 288
BshNI GGYRCC 1 cut(s) 229
BshVI ATCGAT 1 cut(s) 412
BsiEI CGRYCG 1 cut(s) 412
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 4 cut(s) 95, 153, 161, 254
BslFI GGGAC 1 cut(s) 307
BslI CCNNNNNNNGG 3 cut(s) 54, 98, 226
BsmAI GTCTC 2 cut(s) 253, 446
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 307
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 209
Bsp143I GATC 3 cut(s) 42, 409, 440
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 3 cut(s) 15, 399, 401
BspANI GGCC 1 cut(s) 288
BspDI ATCGAT 1 cut(s) 412
BspFNI CGCG 1 cut(s) 401
BspLI GGNNCC 4 cut(s) 231, 258, 323, 467
BspMAI CTGCAG 1 cut(s) 63
BspMI ACCTGC 2 cut(s) 73, 89
BspT107I GGYRCC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 289
BssECI CCNNGG 4 cut(s) 222, 381, 399, 447
BssMI GATC 3 cut(s) 42, 409, 440
BssT1I CCWWGG 2 cut(s) 222, 447
Bst4CI ACNGT 1 cut(s) 337
BstBAI YACGTR 1 cut(s) 418
BstC8I GCNNGC 1 cut(s) 49
BstDSI CCRYGG 3 cut(s) 222, 381, 399
BstENI CCTNNNNNAGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 265
BstFNI CGCG 1 cut(s) 401
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 3 cut(s) 45, 412, 443
BstMAI GTCTC 2 cut(s) 253, 446
BstMBI GATC 3 cut(s) 42, 409, 440
BstMCI CGRYCG 1 cut(s) 412
BstMWI GCNNNNNNNGC 2 cut(s) 67, 435
BstSCI CCNGG 1 cut(s) 160
BstSFI CTRYAG 1 cut(s) 59
BstUI CGCG 1 cut(s) 401
BstV1I GCAGC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 440
BstYI RGATCY 1 cut(s) 440
Bsu15I ATCGAT 1 cut(s) 412
BsuRI GGCC 1 cut(s) 288
BsuTUI ATCGAT 1 cut(s) 412
BtgI CCRYGG 3 cut(s) 222, 381, 399
BtsCI GGATG 1 cut(s) 265
BtsIMutI CAGTG 1 cut(s) 117
BveI ACCTGC 2 cut(s) 73, 89
Cac8I GCNNGC 1 cut(s) 49
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 321
Cfr42I CCGCGG 1 cut(s) 402
ClaI ATCGAT 1 cut(s) 412
Csp6I GTAC 1 cut(s) 172
CviAII CATG 5 cut(s) 223, 318, 331, 367, 470
CviJI RGCY 8 cut(s) 9, 32, 58, 70, 193, 257, 288, 329
CviKI_1 RGCY 8 cut(s) 9, 32, 58, 70, 193, 257, 288, 329
CviQI GTAC 1 cut(s) 172
DpnI GATC 3 cut(s) 44, 411, 442
DpnII GATC 3 cut(s) 42, 409, 440
EaeI YGGCCR 1 cut(s) 286
EciI GGCGGA 1 cut(s) 4
Eco130I CCWWGG 2 cut(s) 222, 447
Eco47I GGWCC 1 cut(s) 321
Eco57I CTGAAG 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 209
EcoNI CCTNNNNNAGG 1 cut(s) 96
EcoT14I CCWWGG 2 cut(s) 222, 447
ErhI CCWWGG 2 cut(s) 222, 447
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 5 cut(s) 226, 321, 334, 370, 473
FaiI YATR 9 cut(s) 224, 279, 291, 310, 312, 319, 332, 368, 471
FaqI GGGAC 1 cut(s) 307
FatI CATG 5 cut(s) 222, 317, 330, 366, 469
FauI CCCGC 1 cut(s) 406
Fnu4HI GCNGC 1 cut(s) 59
FokI GGATG 1 cut(s) 252
Fsp4HI GCNGC 1 cut(s) 59
FspBI CTAG 1 cut(s) 6
GlaI GCGC 1 cut(s) 159
GluI GCNGC 1 cut(s) 59
GsaI CCCAGC 1 cut(s) 329
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 1 cut(s) 288
HapII CCGG 4 cut(s) 95, 153, 161, 254
HhaI GCGC 1 cut(s) 160
Hin1II CATG 5 cut(s) 226, 321, 334, 370, 473
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 2 cut(s) 88, 314
HpaII CCGG 4 cut(s) 95, 153, 161, 254
HphI GGTGA 3 cut(s) 97, 142, 250
Hpy188I TCNGA 1 cut(s) 132
HpyCH4III ACNGT 1 cut(s) 337
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 4 cut(s) 51, 61, 265, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 435
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 5 cut(s) 226, 321, 334, 370, 473
HspAI GCGC 1 cut(s) 158
KspI CCGCGG 1 cut(s) 402
Kzo9I GATC 3 cut(s) 42, 409, 440
LmnI GCTCC 4 cut(s) 14, 155, 198, 262
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 1 cut(s) 274
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 3 cut(s) 107, 115, 148
MalI GATC 3 cut(s) 44, 411, 442
MboI GATC 3 cut(s) 42, 409, 440
MboII GAAGA 2 cut(s) 119, 433
MflI RGATCY 1 cut(s) 440
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 1 cut(s) 182
MluNI TGGCCA 1 cut(s) 288
MnlI CCTC 4 cut(s) 22, 102, 220, 270
Mox20I TGGCCA 1 cut(s) 288
MscI TGGCCA 1 cut(s) 288
MseI TTAA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 288
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 4 cut(s) 95, 153, 161, 254
MspR9I CCNGG 1 cut(s) 162
Mva1269I GAATGC 1 cut(s) 357
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 2 cut(s) 67, 435
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 1 cut(s) 222
NdeII GATC 3 cut(s) 42, 409, 440
NlaIII CATG 5 cut(s) 226, 321, 334, 370, 473
NlaIV GGNNCC 4 cut(s) 231, 258, 323, 467
NmuCI GTSAC 3 cut(s) 107, 115, 148
PaqCI CACCTGC 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PctI GAATGC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 88, 314
PkrI GCNGC 1 cut(s) 60
Ple19I CGATCG 1 cut(s) 412
Ppu21I YACGTR 1 cut(s) 418
PspFI CCCAGC 1 cut(s) 325
PspN4I GGNNCC 4 cut(s) 231, 258, 323, 467
PspPI GGNCC 1 cut(s) 321
PstI CTGCAG 1 cut(s) 63
PsuI RGATCY 1 cut(s) 440
PvuI CGATCG 1 cut(s) 412
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
SacII CCGCGG 1 cut(s) 402
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 59
Sau3AI GATC 3 cut(s) 42, 409, 440
Sau96I GGNCC 1 cut(s) 321
ScrFI CCNGG 1 cut(s) 162
SetI ASST 7 cut(s) 11, 72, 87, 103, 195, 240, 420
SfaNI GCATC 1 cut(s) 274
SfcI CTRYAG 1 cut(s) 59
Sfr303I CCGCGG 1 cut(s) 402
SgrBI CCGCGG 1 cut(s) 402
SinI GGWCC 1 cut(s) 321
Sse9I AATT 1 cut(s) 182
SsiI CCGC 3 cut(s) 15, 399, 401
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 160
StyI CCWWGG 2 cut(s) 222, 447
TaaI ACNGT 1 cut(s) 337
TaiI ACGT 1 cut(s) 420
TaqI TCGA 3 cut(s) 144, 374, 412
TasI AATT 1 cut(s) 182
TfiI GAWTC 2 cut(s) 88, 314
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 3 cut(s) 107, 115, 148
TseI GCWGC 1 cut(s) 58
Tsp45I GTSAC 3 cut(s) 107, 115, 148
TspDTI ATGAA 1 cut(s) 306
TspGWI ACGGA 1 cut(s) 398
TspRI CASTG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 321
XagI CCTNNNNNAGG 1 cut(s) 96
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.