Rorug06G0309400

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002741
Physical Location & Seq
Reverse (-)
46071540 .. 46072211
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0309400.1

Sequence Viewer

Length: 531 bp
ATGGTGTCAAACTTCAAATCCCAATTGTTGATCTCAAGAGGCATTGGCAATGATCACGCTCGGGAAGGAATGGTGAAGGCAATTAAAGATGCATGTGAAACATGGAGGTTCTTTCAGATGGTTTACCATGGAGTTCCACTTGCTGTTATGGAGGAGATGCTTGAAGGTATTTGTAGATTTCACGAGCAGCCTGCAGAAGACAAGATGGATTTGTACTCTCGTGACTTCAAGAATTCAGTCAACTTTTATTGCAGTGGAGATCTGAAATTGCGTGCAAAATCAGCAGCAGATTGGAGAGACACCTTATCTTGCAGAGCCGTAGATGATAAATGGGACTTTGAAGCATTGCCTCGAGTGTGCAGACGTGAAATAAGTGAGTATATTAAAAACTTAATTGAACTTCAAAATGTGCTATCCGAATTACCATTGGAAGCATTAGGGCTTAGCAAGGATCACCTAGCACGCATGGGGTGCTTGAAAGCTTTCTCATTGTCATGCCACTATTATCCAATTTCCCCGAGCCTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

176

Amino Acids

20.25

Weight (kDa)

6.21

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 17 - 107 1.4e-14 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 459
AcsI RAATTY 1 cut(s) 232
AfaI GTAC 1 cut(s) 215
AgsI TTSAA 7 cut(s) 16, 164, 229, 341, 398, 404, 478
AjiI CACGTC 1 cut(s) 365
AluBI AGCT 1 cut(s) 482
AluI AGCT 1 cut(s) 482
Alw26I GTCTC 1 cut(s) 291
AlwI GGATC 1 cut(s) 459
Ama87I CYCGRG 3 cut(s) 60, 351, 517
ApeKI GCWGC 2 cut(s) 187, 284
ApoI RAATTY 1 cut(s) 232
Asp700I GAANNNNTTC 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 85, 446
AvaI CYCGRG 3 cut(s) 60, 351, 517
BauI CACGAG 2 cut(s) 182, 219
BbsI GAAGAC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 199, 296
BccI CCATC 2 cut(s) 112, 199
BceAI ACGGC 1 cut(s) 302
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 291
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 192
BglII AGATCT 1 cut(s) 259
BisI GCNGC 2 cut(s) 188, 285
BlpI GCTNAGC 1 cut(s) 443
BlsI GCNGC 2 cut(s) 189, 286
BmeT110I CYCGRG 3 cut(s) 60, 351, 517
BmgBI CACGTC 1 cut(s) 365
BmsI GCATC 2 cut(s) 79, 147
BpiI GAAGAC 1 cut(s) 204
Bpu1102I GCTNAGC 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 19
BsaJI CCNNGG 1 cut(s) 127
Bse3DI GCAATG 2 cut(s) 55, 344
BseDI CCNNGG 1 cut(s) 127
BseMI GCAATG 2 cut(s) 55, 344
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 2 cut(s) 199, 296
BsgI GTGCAG 1 cut(s) 379
BsiHKCI CYCGRG 3 cut(s) 60, 351, 517
BslFI GGGAC 1 cut(s) 347
BsmAI GTCTC 1 cut(s) 291
BsmFI GGGAC 1 cut(s) 347
BsoBI CYCGRG 3 cut(s) 60, 351, 517
Bsp143I GATC 4 cut(s) 30, 52, 259, 451
Bsp1720I GCTNAGC 1 cut(s) 443
Bsp19I CCATGG 1 cut(s) 127
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 1 cut(s) 459
BsrDI GCAATG 2 cut(s) 55, 344
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 4 cut(s) 30, 52, 259, 451
BssSI CACGAG 2 cut(s) 182, 219
BssT1I CCWWGG 1 cut(s) 127
Bst2BI CACGAG 2 cut(s) 182, 219
BstAPI GCANNNNNTGC 1 cut(s) 471
BstC8I GCNNGC 3 cut(s) 192, 273, 463
BstDEI CTNAG 1 cut(s) 443
BstDSI CCRYGG 1 cut(s) 127
BstKTI GATC 4 cut(s) 33, 55, 262, 454
BstMAI GTCTC 1 cut(s) 291
BstMBI GATC 4 cut(s) 30, 52, 259, 451
BstMWI GCNNNNNNNGC 2 cut(s) 281, 471
BstNSI RCATGY 1 cut(s) 96
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 199, 296
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BtgI CCRYGG 1 cut(s) 127
BtrI CACGTC 1 cut(s) 365
BtsI GCAGTG 1 cut(s) 259
BtsIMutI CAGTG 1 cut(s) 259
Cac8I GCNNGC 3 cut(s) 192, 273, 463
Csp6I GTAC 1 cut(s) 214
CviAII CATG 5 cut(s) 93, 102, 128, 466, 495
CviJI RGCY 5 cut(s) 190, 317, 442, 482, 522
CviKI_1 RGCY 5 cut(s) 190, 317, 442, 482, 522
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 1 cut(s) 443
DpnI GATC 4 cut(s) 32, 54, 261, 453
DpnII GATC 4 cut(s) 30, 52, 259, 451
Eco130I CCWWGG 1 cut(s) 127
Eco88I CYCGRG 3 cut(s) 60, 351, 517
EcoRI GAATTC 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 127
EcoT22I ATGCAT 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 127
FaeI CATG 5 cut(s) 96, 105, 131, 469, 498
FaiI YATR 7 cut(s) 94, 103, 129, 149, 381, 467, 496
FaqI GGGAC 1 cut(s) 347
FatI CATG 5 cut(s) 92, 101, 127, 465, 494
FbaI TGATCA 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 188, 285
Fsp4HI GCNGC 2 cut(s) 188, 285
FspBI CTAG 1 cut(s) 458
GluI GCNGC 2 cut(s) 188, 285
Hin1II CATG 5 cut(s) 96, 105, 131, 469, 498
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HindIII AAGCTT 1 cut(s) 480
HphI GGTGA 2 cut(s) 85, 446
Hpy166II GTNNAC 2 cut(s) 124, 241
Hpy188I TCNGA 3 cut(s) 117, 264, 418
Hpy188III TCNNGA 5 cut(s) 36, 62, 182, 221, 229
Hpy8I GTNNAC 2 cut(s) 124, 241
HpyAV CCTTC 3 cut(s) 59, 70, 158
HpyCH4IV ACGT 1 cut(s) 364
HpyCH4V TGCA 6 cut(s) 92, 194, 252, 275, 312, 360
HpyF10VI GCNNNNNNNGC 2 cut(s) 281, 471
HpyF3I CTNAG 1 cut(s) 443
HpySE526I ACGT 1 cut(s) 364
Hsp92II CATG 5 cut(s) 96, 105, 131, 469, 498
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 4 cut(s) 30, 52, 259, 451
LpnPI CCDG 1 cut(s) 204
Lsp1109I GCAGC 2 cut(s) 199, 296
LweI GCATC 2 cut(s) 79, 147
MaeI CTAG 1 cut(s) 458
MaeII ACGT 1 cut(s) 364
MaeIII GTNAC 1 cut(s) 221
MalI GATC 4 cut(s) 32, 54, 261, 453
MboI GATC 4 cut(s) 30, 52, 259, 451
MboII GAAGA 1 cut(s) 209
MfeI CAATTG 1 cut(s) 23
MflI RGATCY 1 cut(s) 259
MluCI AATT 7 cut(s) 23, 81, 232, 266, 393, 419, 510
MnlI CCTC 4 cut(s) 32, 99, 145, 360
Mph1103I ATGCAT 1 cut(s) 94
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 3 cut(s) 84, 384, 392
MslI CAYNNNNRTG 1 cut(s) 493
MunI CAATTG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 281, 471
NcoI CCATGG 1 cut(s) 127
NdeII GATC 4 cut(s) 30, 52, 259, 451
NlaIII CATG 5 cut(s) 96, 105, 131, 469, 498
NmuCI GTSAC 1 cut(s) 221
NsiI ATGCAT 1 cut(s) 94
NspI RCATGY 1 cut(s) 96
PaeR7I CTCGAG 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 482
PkrI GCNGC 2 cut(s) 189, 286
PspXI VCTCGAGB 1 cut(s) 351
PstI CTGCAG 1 cut(s) 196
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
RseI CAYNNNNRTG 1 cut(s) 493
SaqAI TTAA 3 cut(s) 84, 384, 392
SatI GCNGC 2 cut(s) 188, 285
Sau3AI GATC 4 cut(s) 30, 52, 259, 451
SetI ASST 6 cut(s) 110, 169, 305, 367, 459, 484
SfaNI GCATC 2 cut(s) 79, 147
SfcI CTRYAG 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 351
SlaI CTCGAG 1 cut(s) 351
SmiMI CAYNNNNRTG 1 cut(s) 493
SmlI CTYRAG 2 cut(s) 34, 351
SmoI CTYRAG 2 cut(s) 34, 351
Sse9I AATT 7 cut(s) 23, 81, 232, 266, 393, 419, 510
SspMI CTAG 1 cut(s) 458
StyI CCWWGG 1 cut(s) 127
TaiI ACGT 1 cut(s) 367
TaqI TCGA 1 cut(s) 352
TasI AATT 7 cut(s) 23, 81, 232, 266, 393, 419, 510
TatI WGTACW 1 cut(s) 213
Tru1I TTAA 3 cut(s) 84, 384, 392
Tru9I TTAA 3 cut(s) 84, 384, 392
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 2 cut(s) 187, 284
Tsp45I GTSAC 1 cut(s) 221
TspRI CASTG 1 cut(s) 259
XapI RAATTY 1 cut(s) 232
XceI RCATGY 1 cut(s) 96
XhoI CTCGAG 1 cut(s) 351
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 1 cut(s) 458
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.