Rh6DG421500

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002741
Physical Location & Seq
Forward (+)
60311746 .. 60314096
2351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG421500.1

Sequence Viewer

Length: 486 bp
ATGACCAGCTCCTCTGCCGCTTCACGCAAGGCTTTAAGCAAGATCGCCTGCAATCGGCTCCAGAAGGAGCTCGTGGAGTGGCAGTTGAATCCTCCGGCCGGATTCAAACACAAAGTCACTGATAATCTTCAAAGATGGGTGATTGAAGTGACCGGAGCTCCGGGGACTCTGTACGCCAATGAAATGTATCAGCTCCAAGTTGATTTTCCGGAGCATTATCCCATGGAAGCTCCACAGGTGATTTTTCTCCCTCCAGCTCCTCAGCATCCCCACATTTATAGCAATGGCCATATCTGTTTAGATATATTATATGACTCATGGTCTCCAGCCATGACTGTCAGTTCTATCTGTATCAGCATTCTCTCCATGTTGTCAAGTTCCCCTGCAAAGCAACGCCCAGCGGATAATGATCGATATGTGAAGAACTGTAGGAATGGCAGATCTCCCAAGGAGACGAGGTGGTGGTTCCATGATGACAAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

161

Amino Acids

18.34

Weight (kDa)

8.35

Isoelectric Point (pI)

72.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 19 - 154 2.3e-39 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 208
AciI CCGC 2 cut(s) 18, 401
AcoI YGGCCR 2 cut(s) 96, 286
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 2 cut(s) 54, 98
AgsI TTSAA 4 cut(s) 88, 106, 131, 146
AhdI GACNNNNNGTC 1 cut(s) 319
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AluBI AGCT 6 cut(s) 9, 70, 158, 193, 230, 257
AluI AGCT 6 cut(s) 9, 70, 158, 193, 230, 257
Alw21I GWGCWC 2 cut(s) 72, 160
Alw26I GTCTC 2 cut(s) 327, 446
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 2 cut(s) 96, 286
ArsI GACNNNNNNTTYG 2 cut(s) 99, 131
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 2 cut(s) 151, 250
BalI TGGCCA 1 cut(s) 288
BanII GRGCYC 2 cut(s) 72, 160
BauI CACGAG 1 cut(s) 71
Bbv12I GWGCWC 2 cut(s) 72, 160
BbvCI CCTCAGC 1 cut(s) 261
BccI CCATC 1 cut(s) 129
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 2 cut(s) 327, 446
BfmI CTRYAG 1 cut(s) 427
BglII AGATCT 1 cut(s) 440
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 1 cut(s) 162
BmeRI GACNNNNNGTC 1 cut(s) 319
BmiI GGNNCC 2 cut(s) 59, 467
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 1 cut(s) 274
BpmI CTGGAG 3 cut(s) 44, 237, 309
Bpu10I CCTNAGC 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 162
Bsa29I ATCGAT 1 cut(s) 412
BsaBI GATNNNNATC 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 327
BsaJI CCNNGG 3 cut(s) 161, 222, 447
BsaWI WCCGGW 2 cut(s) 152, 208
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 142, 172, 443, 473
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 98
Bse3DI GCAATG 1 cut(s) 289
Bse8I GATNNNNATC 1 cut(s) 408
BseAI TCCGGA 1 cut(s) 208
BseCI ATCGAT 1 cut(s) 412
BseDI CCNNGG 3 cut(s) 161, 222, 447
BseGI GGATG 1 cut(s) 265
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 54, 98
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 275
BseRI GAGGAG 1 cut(s) 249
BseX3I CGGCCG 1 cut(s) 96
BseYI CCCAGC 1 cut(s) 397
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 2 cut(s) 98, 288
BshVI ATCGAT 1 cut(s) 412
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 2 cut(s) 72, 160
BsiSI CCGG 5 cut(s) 95, 99, 153, 161, 209
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 2 cut(s) 54, 98
BsmAI GTCTC 2 cut(s) 327, 446
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 178
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 2 cut(s) 98, 288
Bso31I GGTCTC 1 cut(s) 327
Bsp1286I GDGCHC 2 cut(s) 72, 160
Bsp13I TCCGGA 1 cut(s) 208
Bsp143I GATC 3 cut(s) 42, 409, 440
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 2 cut(s) 18, 401
BspANI GGCC 2 cut(s) 98, 288
BspCNI CTCAG 1 cut(s) 274
BspDI ATCGAT 1 cut(s) 412
BspEI TCCGGA 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 59, 467
BspTNI GGTCTC 1 cut(s) 327
BsrDI GCAATG 1 cut(s) 289
BssECI CCNNGG 3 cut(s) 161, 222, 447
BssMI GATC 3 cut(s) 42, 409, 440
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 2 cut(s) 222, 447
Bst2BI CACGAG 1 cut(s) 71
Bst4CI ACNGT 2 cut(s) 337, 428
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 1 cut(s) 261
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 265
BstKTI GATC 3 cut(s) 45, 412, 443
BstMAI GTCTC 2 cut(s) 327, 446
BstMBI GATC 3 cut(s) 42, 409, 440
BstMCI CGRYCG 1 cut(s) 99
BstSCI CCNGG 1 cut(s) 160
BstSFI CTRYAG 1 cut(s) 427
BstX2I RGATCY 1 cut(s) 440
BstYI RGATCY 1 cut(s) 440
BstZI CGGCCG 1 cut(s) 96
Bsu15I ATCGAT 1 cut(s) 412
BsuRI GGCC 2 cut(s) 98, 288
BsuTUI ATCGAT 1 cut(s) 412
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 265
BtsIMutI CAGTG 1 cut(s) 117
Cac8I GCNNGC 1 cut(s) 49
ClaI ATCGAT 1 cut(s) 412
Csp6I GTAC 1 cut(s) 172
CviAII CATG 5 cut(s) 223, 318, 331, 367, 470
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 1 cut(s) 261
DpnI GATC 3 cut(s) 44, 411, 442
DpnII GATC 3 cut(s) 42, 409, 440
DriI GACNNNNNGTC 1 cut(s) 319
EaeI YGGCCR 2 cut(s) 96, 286
EagI CGGCCG 1 cut(s) 96
Eam1105I GACNNNNNGTC 1 cut(s) 319
Ecl136II GAGCTC 2 cut(s) 70, 158
EclXI CGGCCG 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 222, 447
Eco24I GRGCYC 2 cut(s) 72, 160
Eco31I GGTCTC 1 cut(s) 327
Eco52I CGGCCG 1 cut(s) 96
Eco53kI GAGCTC 2 cut(s) 70, 158
EcoICRI GAGCTC 2 cut(s) 70, 158
EcoT14I CCWWGG 2 cut(s) 222, 447
EcoT38I GRGCYC 2 cut(s) 72, 160
ErhI CCWWGG 2 cut(s) 222, 447
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 5 cut(s) 226, 321, 334, 370, 473
FaqI GGGAC 1 cut(s) 178
FatI CATG 5 cut(s) 222, 317, 330, 366, 469
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 1 cut(s) 252
FriOI GRGCYC 2 cut(s) 72, 160
Fsp4HI GCNGC 1 cut(s) 18
GluI GCNGC 1 cut(s) 18
GsaI CCCAGC 1 cut(s) 401
GsuI CTGGAG 3 cut(s) 44, 237, 309
HaeIII GGCC 2 cut(s) 98, 288
HapII CCGG 5 cut(s) 95, 99, 153, 161, 209
Hin1II CATG 5 cut(s) 226, 321, 334, 370, 473
HinfI GANTC 4 cut(s) 88, 102, 166, 314
HpaII CCGG 5 cut(s) 95, 99, 153, 161, 209
HphI GGTGA 2 cut(s) 151, 250
Hpy188III TCNNGA 2 cut(s) 61, 209
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 2 cut(s) 337, 428
HpyCH4V TGCA 2 cut(s) 51, 386
HpyF3I CTNAG 1 cut(s) 261
Hsp92II CATG 5 cut(s) 226, 321, 334, 370, 473
Kpn2I TCCGGA 1 cut(s) 208
Kzo9I GATC 3 cut(s) 42, 409, 440
LmnI GCTCC 9 cut(s) 14, 63, 67, 155, 163, 198, 211, 235, 262
LweI GCATC 1 cut(s) 274
MaeIII GTNAC 2 cut(s) 115, 148
MalI GATC 3 cut(s) 44, 411, 442
MboI GATC 3 cut(s) 42, 409, 440
MboII GAAGA 2 cut(s) 119, 433
MflI RGATCY 1 cut(s) 440
MhlI GDGCHC 2 cut(s) 72, 160
MlsI TGGCCA 1 cut(s) 288
MluNI TGGCCA 1 cut(s) 288
MlyI GAGTC 2 cut(s) 160, 308
MnlI CCTC 5 cut(s) 22, 102, 261, 270, 450
Mox20I TGGCCA 1 cut(s) 288
MroI TCCGGA 1 cut(s) 208
MscI TGGCCA 1 cut(s) 288
MseI TTAA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 288
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 5 cut(s) 95, 99, 153, 161, 209
MspR9I CCNGG 1 cut(s) 162
Mva1269I GAATGC 1 cut(s) 357
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 1 cut(s) 222
NdeII GATC 3 cut(s) 42, 409, 440
NlaIII CATG 5 cut(s) 226, 321, 334, 370, 473
NlaIV GGNNCC 2 cut(s) 59, 467
NmuCI GTSAC 2 cut(s) 115, 148
PctI GAATGC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 88, 102
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 2 cut(s) 160, 308
PpsI GAGTC 2 cut(s) 160, 308
Psp124BI GAGCTC 2 cut(s) 72, 160
PspFI CCCAGC 1 cut(s) 397
PspN4I GGNNCC 2 cut(s) 59, 467
PsuI RGATCY 1 cut(s) 440
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
SacI GAGCTC 2 cut(s) 72, 160
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 3 cut(s) 42, 409, 440
SchI GAGTC 2 cut(s) 160, 308
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 2 cut(s) 72, 160
SetI ASST 9 cut(s) 11, 72, 160, 195, 232, 240, 259, 461, 483
SfaNI GCATC 1 cut(s) 274
SfcI CTRYAG 1 cut(s) 427
SsiI CCGC 2 cut(s) 18, 401
SstI GAGCTC 2 cut(s) 72, 160
StyD4I CCNGG 1 cut(s) 160
StyI CCWWGG 2 cut(s) 222, 447
TaaI ACNGT 2 cut(s) 337, 428
TaqI TCGA 1 cut(s) 412
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 2 cut(s) 88, 102
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 2 cut(s) 115, 148
Tsp45I GTSAC 2 cut(s) 115, 148
TspDTI ATGAA 1 cut(s) 195
TspRI CASTG 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.