pycom08g01890

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002741
Physical Location & Seq
Reverse (-)
1400416 .. 1401188
773 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01890.1

Sequence Viewer

Length: 321 bp
ATGACTAGCTCCTCCTCCACTTCACGCAAGGCTTTAAGCAAGATCGCCTGCAATCGTCTGCAGAAAGAGCTGGTGGAGTGGCAGGTGAATGCTCCGGCAGGTTTTAGTCACAAAGTCACTGATAATCTTCAGAGATGGGTAATCGAAGTCACCGGAGCTCCGGGAACTCTATACGCTAACGAATTGTATCAGCTCCAAGTTGATTTCCCCGAGCATTACCCCATGGAAGCACCACAGGTGATTTTTGTCTCTCCGGCTCCTCTGCATCCTCACATTTATAGCAATGGCCATATTTGTTTAGGTACCGTTCTTGTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

107

Amino Acids

11.82

Weight (kDa)

6.49

Isoelectric Point (pI)

64.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 19 - 100 1.9e-24 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 73
Acc36I ACCTGC 2 cut(s) 73, 89
Acc65I GGTACC 1 cut(s) 302
AccB1I GGYRCC 1 cut(s) 302
AcoI YGGCCR 1 cut(s) 286
AcuI CTGAAG 1 cut(s) 113
AfaI GTAC 1 cut(s) 304
AluBI AGCT 4 cut(s) 9, 70, 158, 193
AluI AGCT 4 cut(s) 9, 70, 158, 193
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 1 cut(s) 253
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 1 cut(s) 286
Asp718I GGTACC 1 cut(s) 302
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 3 cut(s) 97, 142, 250
AvaI CYCGRG 1 cut(s) 209
BalI TGGCCA 1 cut(s) 288
BanI GGYRCC 1 cut(s) 302
BanII GRGCYC 1 cut(s) 160
Bbv12I GWGCWC 1 cut(s) 160
BccI CCATC 1 cut(s) 129
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 59
BfuAI ACCTGC 2 cut(s) 73, 89
Bme1390I CCNGG 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 209
BmiI GGNNCC 2 cut(s) 258, 304
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 1 cut(s) 274
BpuMI CCSGG 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 222
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bse3DI GCAATG 1 cut(s) 289
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 1 cut(s) 265
BseMI GCAATG 1 cut(s) 289
BseRI GAGGAG 2 cut(s) 4, 249
BshFI GGCC 1 cut(s) 288
BshNI GGYRCC 1 cut(s) 302
BsiHKAI GWGCWC 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 4 cut(s) 95, 153, 161, 254
BsmAI GTCTC 1 cut(s) 253
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 160
Bsp143I GATC 1 cut(s) 42
Bsp19I CCATGG 1 cut(s) 222
BspANI GGCC 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 258, 304
BspMAI CTGCAG 1 cut(s) 63
BspMI ACCTGC 2 cut(s) 73, 89
BspT107I GGYRCC 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 289
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 222
Bst4CI ACNGT 1 cut(s) 307
BstC8I GCNNGC 1 cut(s) 49
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 265
BstKTI GATC 1 cut(s) 45
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 1 cut(s) 42
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstSCI CCNGG 1 cut(s) 160
BstSFI CTRYAG 1 cut(s) 59
BsuRI GGCC 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 265
BtsIMutI CAGTG 1 cut(s) 117
BveI ACCTGC 2 cut(s) 73, 89
Cac8I GCNNGC 1 cut(s) 49
Csp6I GTAC 1 cut(s) 303
CviAII CATG 1 cut(s) 223
CviJI RGCY 7 cut(s) 9, 32, 70, 158, 193, 257, 288
CviKI_1 RGCY 7 cut(s) 9, 32, 70, 158, 193, 257, 288
CviQI GTAC 1 cut(s) 303
DpnI GATC 1 cut(s) 44
DpnII GATC 1 cut(s) 42
EaeI YGGCCR 1 cut(s) 286
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 1 cut(s) 226
FaiI YATR 4 cut(s) 172, 224, 279, 291
FatI CATG 1 cut(s) 222
FokI GGATG 1 cut(s) 252
FriOI GRGCYC 1 cut(s) 160
FspBI CTAG 1 cut(s) 6
HaeIII GGCC 1 cut(s) 288
HapII CCGG 4 cut(s) 95, 153, 161, 254
Hin1II CATG 1 cut(s) 226
HpaII CCGG 4 cut(s) 95, 153, 161, 254
HphI GGTGA 3 cut(s) 97, 142, 250
Hpy188I TCNGA 1 cut(s) 132
HpyCH4III ACNGT 1 cut(s) 307
HpyCH4V TGCA 3 cut(s) 51, 61, 265
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
Hsp92II CATG 1 cut(s) 226
KpnI GGTACC 1 cut(s) 306
Kzo9I GATC 1 cut(s) 42
LmnI GCTCC 6 cut(s) 14, 97, 155, 163, 198, 262
LpnPI CCDG 9 cut(s) 56, 61, 68, 84, 108, 166, 174, 221, 267
LweI GCATC 1 cut(s) 274
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 3 cut(s) 107, 115, 148
MalI GATC 1 cut(s) 44
MboI GATC 1 cut(s) 42
MboII GAAGA 1 cut(s) 119
MhlI GDGCHC 1 cut(s) 160
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 1 cut(s) 182
MluNI TGGCCA 1 cut(s) 288
MnlI CCTC 4 cut(s) 22, 25, 270, 279
Mox20I TGGCCA 1 cut(s) 288
MscI TGGCCA 1 cut(s) 288
MseI TTAA 2 cut(s) 35, 319
Msp20I TGGCCA 1 cut(s) 288
MspI CCGG 4 cut(s) 95, 153, 161, 254
MspR9I CCNGG 1 cut(s) 162
Mva1269I GAATGC 1 cut(s) 94
MwoI GCNNNNNNNGC 1 cut(s) 67
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 1 cut(s) 222
NdeII GATC 1 cut(s) 42
NlaIII CATG 1 cut(s) 226
NlaIV GGNNCC 2 cut(s) 258, 304
NmuCI GTSAC 3 cut(s) 107, 115, 148
PaqCI CACCTGC 1 cut(s) 73
PctI GAATGC 1 cut(s) 94
PfoI TCCNGGA 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 160
PspN4I GGNNCC 2 cut(s) 258, 304
PstI CTGCAG 1 cut(s) 63
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
SacI GAGCTC 1 cut(s) 160
SaqAI TTAA 2 cut(s) 35, 319
Sau3AI GATC 1 cut(s) 42
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 1 cut(s) 160
SetI ASST 8 cut(s) 11, 72, 87, 103, 160, 195, 240, 304
SfaNI GCATC 1 cut(s) 274
SfcI CTRYAG 1 cut(s) 59
Sse9I AATT 1 cut(s) 182
SspMI CTAG 1 cut(s) 6
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 1 cut(s) 160
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 307
TaqI TCGA 1 cut(s) 144
TasI AATT 1 cut(s) 182
Tru1I TTAA 2 cut(s) 35, 319
Tru9I TTAA 2 cut(s) 35, 319
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 3 cut(s) 107, 115, 148
Tsp45I GTSAC 3 cut(s) 107, 115, 148
TspRI CASTG 1 cut(s) 124
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.