MD15G1021700.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002741
Physical Location & Seq
Reverse (-)
1254520 .. 1257089
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1021700.v1.1.491

Sequence Viewer

Length: 486 bp
ATGACGAGCTCCTCCGCCACTTCACGCAAGGCCTTAAGCAAGATCGCCTGCAATCGGCTGCAGAAAGAGCTGGTGGAGTGGCAGGTGAATCCTCCGGCAGGTTTCAAGCACAAAGTCACCGATAATCTTCAGAGATGGGTCATCGAAGTCACCGGAGCGCCGGGAACTCTATACGCTAACGAAATTTATCAGCTCCAAGTCGATTTCCCCGAGCATTATCCCATGGAAGCACCACAGGTGATTTTTGTCCCTCCGGCTCCTCTGCATCCTCACATTTACAGCAATGGCCATATCTGTTTAGATATTCTATATGATTCATGGTCCCCAGCCATGACGGTTAGTTCCATCTGTATCAGCATTCTCTCCATGTTATCGAGTTCCACGGTGAAGCAACGCCCAGCGGATAACGATCGATACGTGAAGAACTGCAGGAACGGCAGATCTCCCAAGGAGACGACATGGTGGTTCCATGATGACAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

162

Amino Acids

18.29

Weight (kDa)

7.72

Isoelectric Point (pI)

62.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 19 - 153 2.8e-39 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 73
Acc36I ACCTGC 2 cut(s) 73, 89
AciI CCGC 2 cut(s) 15, 401
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 113
AfiI CCNNNNNNNGG 2 cut(s) 54, 98
AflII CTTAAG 1 cut(s) 34
AgsI TTSAA 1 cut(s) 106
AluBI AGCT 3 cut(s) 9, 70, 193
AluI AGCT 3 cut(s) 9, 70, 193
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 2 cut(s) 30, 286
ApeKI GCWGC 1 cut(s) 58
ApoI RAATTY 1 cut(s) 183
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 321
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 5 cut(s) 97, 109, 142, 250, 397
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 321
BalI TGGCCA 1 cut(s) 288
BanII GRGCYC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 1 cut(s) 45
BccI CCATC 2 cut(s) 129, 353
BceAI ACGGC 1 cut(s) 451
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 446
BfmI CTRYAG 2 cut(s) 59, 427
BfoI RGCGCY 1 cut(s) 161
BfrI CTTAAG 1 cut(s) 34
BfuAI ACCTGC 2 cut(s) 73, 89
BglII AGATCT 1 cut(s) 440
BisI GCNGC 1 cut(s) 59
BlsI GCNGC 1 cut(s) 60
Bme1390I CCNGG 1 cut(s) 162
Bme18I GGWCC 1 cut(s) 321
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 321
BmiI GGNNCC 3 cut(s) 258, 323, 467
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 1 cut(s) 274
BpuMI CCSGG 1 cut(s) 162
Bsa29I ATCGAT 1 cut(s) 412
BsaAI YACGTR 1 cut(s) 418
BsaBI GATNNNNATC 1 cut(s) 408
BsaJI CCNNGG 3 cut(s) 222, 381, 447
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 98
Bse3DI GCAATG 1 cut(s) 289
Bse8I GATNNNNATC 1 cut(s) 408
BseCI ATCGAT 1 cut(s) 412
BseDI CCNNGG 3 cut(s) 222, 381, 447
BseGI GGATG 1 cut(s) 265
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 54, 98
BseMI GCAATG 1 cut(s) 289
BseRI GAGGAG 1 cut(s) 249
BseXI GCAGC 1 cut(s) 45
BseYI CCCAGC 2 cut(s) 325, 397
Bsh1285I CGRYCG 1 cut(s) 412
BshFI GGCC 2 cut(s) 32, 288
BshVI ATCGAT 1 cut(s) 412
BsiEI CGRYCG 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 4 cut(s) 95, 153, 161, 254
BslFI GGGAC 2 cut(s) 233, 307
BslI CCNNNNNNNGG 2 cut(s) 54, 98
BsmAI GTCTC 1 cut(s) 446
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 2 cut(s) 233, 307
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 2 cut(s) 32, 288
BsoBI CYCGRG 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 3 cut(s) 42, 409, 440
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 2 cut(s) 15, 401
BspANI GGCC 2 cut(s) 32, 288
BspDI ATCGAT 1 cut(s) 412
BspLI GGNNCC 3 cut(s) 258, 323, 467
BspMAI CTGCAG 2 cut(s) 63, 431
BspMI ACCTGC 2 cut(s) 73, 89
BspTI CTTAAG 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 289
BssECI CCNNGG 3 cut(s) 222, 381, 447
BssMI GATC 3 cut(s) 42, 409, 440
BssT1I CCWWGG 2 cut(s) 222, 447
Bst4CI ACNGT 2 cut(s) 337, 385
BstAFI CTTAAG 1 cut(s) 34
BstBAI YACGTR 1 cut(s) 418
BstC8I GCNNGC 1 cut(s) 49
BstDSI CCRYGG 2 cut(s) 222, 381
BstENI CCTNNNNNAGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 265
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 3 cut(s) 45, 412, 443
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 3 cut(s) 42, 409, 440
BstMCI CGRYCG 1 cut(s) 412
BstMWI GCNNNNNNNGC 2 cut(s) 67, 435
BstSCI CCNGG 1 cut(s) 160
BstSFI CTRYAG 2 cut(s) 59, 427
BstV1I GCAGC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 440
BstYI RGATCY 1 cut(s) 440
Bsu15I ATCGAT 1 cut(s) 412
BsuRI GGCC 2 cut(s) 32, 288
BsuTUI ATCGAT 1 cut(s) 412
BtgI CCRYGG 2 cut(s) 222, 381
BtsCI GGATG 1 cut(s) 265
BveI ACCTGC 2 cut(s) 73, 89
Cac8I GCNNGC 1 cut(s) 49
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 321
ClaI ATCGAT 1 cut(s) 412
CviAII CATG 6 cut(s) 223, 318, 331, 367, 459, 470
CviJI RGCY 8 cut(s) 9, 32, 58, 70, 193, 257, 288, 329
CviKI_1 RGCY 8 cut(s) 9, 32, 58, 70, 193, 257, 288, 329
DpnI GATC 3 cut(s) 44, 411, 442
DpnII GATC 3 cut(s) 42, 409, 440
EaeI YGGCCR 1 cut(s) 286
EciI GGCGGA 1 cut(s) 4
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 2 cut(s) 222, 447
Eco147I AGGCCT 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 321
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 9
EcoNI CCTNNNNNAGG 1 cut(s) 96
EcoT14I CCWWGG 2 cut(s) 222, 447
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 2 cut(s) 222, 447
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 6 cut(s) 226, 321, 334, 370, 462, 473
FaqI GGGAC 2 cut(s) 233, 307
FatI CATG 6 cut(s) 222, 317, 330, 366, 458, 469
Fnu4HI GCNGC 1 cut(s) 59
FokI GGATG 1 cut(s) 252
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 59
GlaI GCGC 1 cut(s) 159
GluI GCNGC 1 cut(s) 59
GsaI CCCAGC 2 cut(s) 329, 401
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 2 cut(s) 32, 288
HapII CCGG 4 cut(s) 95, 153, 161, 254
HhaI GCGC 1 cut(s) 160
Hin1II CATG 6 cut(s) 226, 321, 334, 370, 462, 473
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 2 cut(s) 88, 314
HpaII CCGG 4 cut(s) 95, 153, 161, 254
HphI GGTGA 5 cut(s) 97, 109, 142, 250, 397
Hpy188I TCNGA 1 cut(s) 132
HpyCH4III ACNGT 2 cut(s) 337, 385
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 4 cut(s) 51, 61, 265, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 435
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 6 cut(s) 226, 321, 334, 370, 462, 473
HspAI GCGC 1 cut(s) 158
Kzo9I GATC 3 cut(s) 42, 409, 440
LmnI GCTCC 4 cut(s) 14, 155, 198, 262
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 1 cut(s) 274
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 2 cut(s) 115, 148
MalI GATC 3 cut(s) 44, 411, 442
MboI GATC 3 cut(s) 42, 409, 440
MboII GAAGA 2 cut(s) 119, 433
MflI RGATCY 1 cut(s) 440
MhlI GDGCHC 1 cut(s) 11
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 1 cut(s) 183
MluNI TGGCCA 1 cut(s) 288
MnlI CCTC 5 cut(s) 22, 102, 261, 270, 279
Mox20I TGGCCA 1 cut(s) 288
MscI TGGCCA 1 cut(s) 288
MseI TTAA 2 cut(s) 35, 484
Msp20I TGGCCA 1 cut(s) 288
MspA1I CMGCKG 1 cut(s) 401
MspCI CTTAAG 1 cut(s) 34
MspI CCGG 4 cut(s) 95, 153, 161, 254
MspR9I CCNGG 1 cut(s) 162
Mva1269I GAATGC 1 cut(s) 357
MwoI GCNNNNNNNGC 2 cut(s) 67, 435
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 1 cut(s) 222
NdeII GATC 3 cut(s) 42, 409, 440
NlaIII CATG 6 cut(s) 226, 321, 334, 370, 462, 473
NlaIV GGNNCC 3 cut(s) 258, 323, 467
NmuCI GTSAC 2 cut(s) 115, 148
PaqCI CACCTGC 1 cut(s) 73
PceI AGGCCT 1 cut(s) 32
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 414
PctI GAATGC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 88, 314
PkrI GCNGC 1 cut(s) 60
Ple19I CGATCG 1 cut(s) 412
Ppu21I YACGTR 1 cut(s) 418
Psp124BI GAGCTC 1 cut(s) 11
PspFI CCCAGC 2 cut(s) 325, 397
PspN4I GGNNCC 3 cut(s) 258, 323, 467
PspPI GGNCC 1 cut(s) 321
PstI CTGCAG 2 cut(s) 63, 431
PsuI RGATCY 1 cut(s) 440
PvuI CGATCG 1 cut(s) 412
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 2 cut(s) 35, 484
SatI GCNGC 1 cut(s) 59
Sau3AI GATC 3 cut(s) 42, 409, 440
Sau96I GGNCC 1 cut(s) 321
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 1 cut(s) 11
SetI ASST 8 cut(s) 11, 72, 87, 103, 195, 240, 420, 483
SfaNI GCATC 1 cut(s) 274
SfcI CTRYAG 2 cut(s) 59, 427
SinI GGWCC 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 1 cut(s) 183
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 2 cut(s) 15, 401
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 160
StyI CCWWGG 2 cut(s) 222, 447
TaaI ACNGT 2 cut(s) 337, 385
TaiI ACGT 1 cut(s) 420
TaqI TCGA 4 cut(s) 144, 201, 374, 412
TasI AATT 1 cut(s) 183
TfiI GAWTC 2 cut(s) 88, 314
Tru1I TTAA 2 cut(s) 35, 484
Tru9I TTAA 2 cut(s) 35, 484
TseFI GTSAC 2 cut(s) 115, 148
TseI GCWGC 1 cut(s) 58
Tsp45I GTSAC 2 cut(s) 115, 148
TspDTI ATGAA 1 cut(s) 306
Vha464I CTTAAG 1 cut(s) 34
VpaK11BI GGWCC 1 cut(s) 321
XagI CCTNNNNNAGG 1 cut(s) 96
XapI RAATTY 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.