MD08G1031100.v1.1

Maf-like protein DDB_G0281937 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002073
Physical Location & Seq
Reverse (-)
2219494 .. 2223263
3770 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1031100.v1.1.491

Sequence Viewer

Length: 675 bp
ATGGAAGCCGATTCATCTTCCTTCAAGATAATCTTGGGATCGGCTTCGATAGCACGCCGGAAAATATTGGCTGAAATGGGATATGAATTCACAGTCGTGACTGCAGACATTGATGAAAAATCCATCCGAACGGAAAAACCAGAGGAGTTGGTTTTGGTTCTTGCCAAGGCAAAGGCCGATGCTATTATATCAAAGTTGCAAAGCATCAATAAACAAGAGAAGGATACTGAGCCGACAATTGTGATTGCGGCAGACACAGCAGAAGCCATTTCACAAAAGCTCCCAGTTGGGGACTACATAAAGGATGCCAAACCAACACTATTAGTTACTTCTGATCAAGTGGTGGTCTATGAAGGTGTGATCCGGGAAAAACCATCCAGCAAGGAAGAAGCACGGCAATTTTTGAAAGATTATTCTGGAGGGCATGCAGCAACAGTAGGATCTGTGCATGTTACAAACCTTAAAACGGGATTCAGTAAAGGAGAATGGGATCGAGTGGAGATTTATTTCCATAAAATACCAGATGAAGTCATTGAGAAGCTGATTGAGGAGGGGATAGTGCTCAAAGTTGCTGGGGGACTCATAATCGAACATCCTCTTATTCTTCCCTATGTCAAAGAAGTGCTTTTGTTTAATGAATCCTGCAAGCATAACCCATCGTGGTCGGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Maf

Protein Analysis

225

Amino Acids

24.75

Weight (kDa)

5.26

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Maf PF02545 9 - 207 6.8e-33 Maf-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 248
AclWI GGATC 4 cut(s) 46, 355, 448, 498
AcsI RAATTY 1 cut(s) 86
AfiI CCNNNNNNNGG 2 cut(s) 289, 466
AgsI TTSAA 2 cut(s) 25, 406
AleI CACNNNNGTG 1 cut(s) 95
AluBI AGCT 2 cut(s) 280, 541
AluI AGCT 2 cut(s) 280, 541
Alw21I GWGCWC 1 cut(s) 564
AlwI GGATC 4 cut(s) 46, 355, 448, 498
AoxI GGCC 1 cut(s) 174
ApeKI GCWGC 1 cut(s) 428
ApoI RAATTY 1 cut(s) 86
AsuC2I CCSGG 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 564
BbvI GCAGC 1 cut(s) 440
BccI CCATC 3 cut(s) 131, 382, 664
BceAI ACGGC 1 cut(s) 410
BciVI GTATCC 1 cut(s) 217
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 365
BfmI CTRYAG 1 cut(s) 102
BfuI GTATCC 1 cut(s) 217
BisI GCNGC 2 cut(s) 249, 429
BlsI GCNGC 2 cut(s) 250, 430
Bme1390I CCNGG 1 cut(s) 365
BmrFI CCNGG 1 cut(s) 365
BmrI ACTGGG 1 cut(s) 278
BmsI GCATC 3 cut(s) 169, 213, 295
BmuI ACTGGG 1 cut(s) 278
BpmI CTGGAG 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 466
Bse1I ACTGG 1 cut(s) 284
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 5 cut(s) 123, 310, 374, 592, 673
BseLI CCNNNNNNNGG 2 cut(s) 289, 466
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 284
BseRI GAGGAG 2 cut(s) 158, 563
BseXI GCAGC 1 cut(s) 440
BseYI CCCAGC 1 cut(s) 572
BshFI GGCC 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 564
BsiSI CCGG 2 cut(s) 58, 364
BslFI GGGAC 2 cut(s) 305, 591
BslI CCNNNNNNNGG 2 cut(s) 289, 466
BsmFI GGGAC 2 cut(s) 305, 591
BsnI GGCC 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 564
Bsp143I GATC 5 cut(s) 38, 334, 360, 440, 490
BspACI CCGC 1 cut(s) 248
BspANI GGCC 1 cut(s) 176
BspCNI CTCAG 1 cut(s) 220
BspMAI CTGCAG 1 cut(s) 106
BspPI GGATC 4 cut(s) 46, 355, 448, 498
BsrI ACTGG 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 5 cut(s) 38, 334, 360, 440, 490
BssT1I CCWWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 94, 436
BstC8I GCNNGC 3 cut(s) 55, 426, 647
BstDEI CTNAG 1 cut(s) 228
BstF5I GGATG 5 cut(s) 123, 310, 374, 592, 673
BstKTI GATC 5 cut(s) 41, 337, 363, 443, 493
BstMBI GATC 5 cut(s) 38, 334, 360, 440, 490
BstMWI GCNNNNNNNGC 2 cut(s) 50, 257
BstNSI RCATGY 2 cut(s) 428, 452
BstSCI CCNGG 1 cut(s) 363
BstSFI CTRYAG 1 cut(s) 102
BstV1I GCAGC 1 cut(s) 440
BstX2I RGATCY 1 cut(s) 440
BstYI RGATCY 1 cut(s) 440
BsuI GTATCC 1 cut(s) 217
BsuRI GGCC 1 cut(s) 176
BtsCI GGATG 5 cut(s) 123, 310, 374, 592, 673
Cac8I GCNNGC 3 cut(s) 55, 426, 647
CviAII CATG 2 cut(s) 425, 449
CviJI RGCY 8 cut(s) 8, 44, 71, 176, 232, 266, 280, 541
CviKI_1 RGCY 8 cut(s) 8, 44, 71, 176, 232, 266, 280, 541
DdeI CTNAG 1 cut(s) 228
DpnI GATC 5 cut(s) 40, 336, 362, 442, 492
DpnII GATC 5 cut(s) 38, 334, 360, 440, 490
Eco130I CCWWGG 1 cut(s) 165
EcoRI GAATTC 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 2 cut(s) 428, 452
FalI AAGNNNNNCTT 4 cut(s) 17, 49, 609, 641
FaqI GGGAC 2 cut(s) 305, 591
FatI CATG 2 cut(s) 424, 448
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 249, 429
FokI GGATG 4 cut(s) 110, 317, 361, 579
Fsp4HI GCNGC 2 cut(s) 249, 429
GluI GCNGC 2 cut(s) 249, 429
GsaI CCCAGC 1 cut(s) 576
GsuI CTGGAG 1 cut(s) 438
HaeIII GGCC 1 cut(s) 176
HapII CCGG 2 cut(s) 58, 364
Hin1II CATG 2 cut(s) 428, 452
HinfI GANTC 4 cut(s) 11, 471, 579, 638
HpaII CCGG 2 cut(s) 58, 364
Hpy188I TCNGA 3 cut(s) 128, 334, 667
Hpy188III TCNNGA 3 cut(s) 25, 97, 417
HpyAV CCTTC 3 cut(s) 31, 214, 347
HpyCH4III ACNGT 2 cut(s) 94, 436
HpyCH4V TGCA 5 cut(s) 104, 199, 428, 448, 645
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 257
HpyF3I CTNAG 1 cut(s) 228
Hsp92II CATG 2 cut(s) 428, 452
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 5 cut(s) 38, 334, 360, 440, 490
LmnI GCTCC 1 cut(s) 285
LpnPI CCDG 9 cut(s) 71, 153, 297, 377, 391, 402, 534, 558, 655
Lsp1109I GCAGC 1 cut(s) 440
LweI GCATC 3 cut(s) 169, 213, 295
MaeIII GTNAC 3 cut(s) 97, 325, 451
MalI GATC 5 cut(s) 40, 336, 362, 442, 492
MboI GATC 5 cut(s) 38, 334, 360, 440, 490
MboII GAAGA 3 cut(s) 9, 398, 596
MfeI CAATTG 1 cut(s) 237
MflI RGATCY 1 cut(s) 440
MhlI GDGCHC 1 cut(s) 564
MluCI AATT 3 cut(s) 86, 237, 398
MlyI GAGTC 1 cut(s) 573
MmeI TCCRAC 1 cut(s) 645
MnlI CCTC 5 cut(s) 136, 413, 541, 544, 606
MseI TTAA 2 cut(s) 462, 633
MslI CAYNNNNRTG 1 cut(s) 95
MspI CCGG 2 cut(s) 58, 364
MspR9I CCNGG 1 cut(s) 365
MunI CAATTG 1 cut(s) 237
MwoI GCNNNNNNNGC 2 cut(s) 50, 257
NciI CCSGG 1 cut(s) 365
NdeII GATC 5 cut(s) 38, 334, 360, 440, 490
NlaIII CATG 2 cut(s) 428, 452
NmuCI GTSAC 1 cut(s) 97
NspI RCATGY 2 cut(s) 428, 452
OliI CACNNNNGTG 1 cut(s) 95
PaeI GCATGC 1 cut(s) 428
PfeI GAWTC 3 cut(s) 11, 471, 638
PfoI TCCNGGA 1 cut(s) 363
PkrI GCNGC 2 cut(s) 250, 430
PleI GAGTC 1 cut(s) 573
PpsI GAGTC 1 cut(s) 573
PspFI CCCAGC 1 cut(s) 572
PstI CTGCAG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 440
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 2 cut(s) 462, 633
SatI GCNGC 2 cut(s) 249, 429
Sau3AI GATC 5 cut(s) 38, 334, 360, 440, 490
SchI GAGTC 1 cut(s) 573
ScrFI CCNGG 1 cut(s) 365
SduI GDGCHC 1 cut(s) 564
SetI ASST 4 cut(s) 282, 358, 462, 543
SfaNI GCATC 3 cut(s) 169, 213, 295
SfcI CTRYAG 1 cut(s) 102
SmiMI CAYNNNNRTG 1 cut(s) 95
SphI GCATGC 1 cut(s) 428
Sse9I AATT 3 cut(s) 86, 237, 398
SsiI CCGC 1 cut(s) 248
SspI AATATT 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 363
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 94, 436
TaqI TCGA 3 cut(s) 47, 493, 588
TasI AATT 3 cut(s) 86, 237, 398
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 3 cut(s) 11, 471, 638
Tru1I TTAA 2 cut(s) 462, 633
Tru9I TTAA 2 cut(s) 462, 633
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 1 cut(s) 428
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 5 cut(s) 99, 129, 366, 540, 651
TspGWI ACGGA 1 cut(s) 146
XapI RAATTY 1 cut(s) 86
XceI RCATGY 2 cut(s) 428, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.