MD15G1026600.v1.1

Maf-like protein DDB_G0281937 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002073
Physical Location & Seq
Reverse (-)
1566098 .. 1569339
3242 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1026600.v1.1.491

Sequence Viewer

Length: 696 bp
ATGGAAGCCGATTCATCTTCCTTCAAGATAATCTTGGGATCGGCTTCGATAGCACGTCGAAAAATATTGGCTGAAATGGGATATGAATTCACAGTCATGACTGCCGACATTGACGAAAAATCCATCCGAACGGAAAAACCAGAGGAGTTGGTTTTGGTTCTTGCCAAGGCAAAGGCCGATGCTATCGTATCAAAGTTGCAAAGTATCAATAATCAAGAAAAGGATGCTGAGACCACGATTGTGATTGCGGCAGACACAGCAGAAGCCATTTCACAAAGGCTCCCAGTTGGTGACTACATAAAGGATGCCGAACCAACGCTATTAATTACTTCTGATCAAGTGGTGGTCTATGAAGGTGTGATTCGGGAAAAACCATCCGGCAAGGAAGAAGCACGGCAATTTTTGAAAGACTATTCTGGAGGGCATGCAGCAACAGTGGGATCTGTACATGTTACAAACCTTAAAACGGGATTCAGTAAAGGAGAATGGGATCGAGTGGAGATTTATTTCCATAAAATACCAGATGAAGTCATTGAGAAGCTGATTGAGGAGGGGATAGTGCTCAAAGTAGCAGGGGGACTCATAATAGAACATCCTCTAATACTTCCTTTTGTTAAAGAAGTGGTAGGGACAACCGATAGTGTGATGGGACTTCCCATAGATCTCACAAGGAGACTACTGAAAGAGGCCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Maf

Protein Analysis

232

Amino Acids

25.33

Weight (kDa)

5.03

Isoelectric Point (pI)

29.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Maf PF02545 9 - 229 9e-42 Maf-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 248
AclWI GGATC 3 cut(s) 46, 448, 498
AcsI RAATTY 1 cut(s) 86
AfaI GTAC 1 cut(s) 447
AfiI CCNNNNNNNGG 1 cut(s) 466
AflIII ACRYGT 1 cut(s) 448
AgsI TTSAA 2 cut(s) 25, 406
AjiI CACGTC 1 cut(s) 56
AleI CACNNNNGTG 1 cut(s) 239
AluBI AGCT 1 cut(s) 541
AluI AGCT 1 cut(s) 541
Alw21I GWGCWC 1 cut(s) 564
Alw26I GTCTC 2 cut(s) 224, 667
AlwI GGATC 3 cut(s) 46, 448, 498
AoxI GGCC 2 cut(s) 174, 687
ApeKI GCWGC 1 cut(s) 428
ApoI RAATTY 1 cut(s) 86
AseI ATTAAT 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 564
BbvI GCAGC 1 cut(s) 440
BccI CCATC 3 cut(s) 131, 382, 640
BceAI ACGGC 2 cut(s) 410, 674
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 2 cut(s) 224, 667
BfaI CTAG 1 cut(s) 694
BglII AGATCT 1 cut(s) 661
BisI GCNGC 2 cut(s) 249, 429
BlsI GCNGC 2 cut(s) 250, 430
BmgBI CACGTC 1 cut(s) 56
BmiI GGNNCC 1 cut(s) 281
BmrI ACTGGG 1 cut(s) 278
BmsI GCATC 3 cut(s) 169, 214, 295
BmuI ACTGGG 1 cut(s) 278
BpmI CTGGAG 1 cut(s) 438
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse1I ACTGG 1 cut(s) 284
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 5 cut(s) 123, 229, 310, 374, 592
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 284
BseRI GAGGAG 2 cut(s) 158, 563
BseXI GCAGC 1 cut(s) 440
BshFI GGCC 2 cut(s) 176, 689
BsiHKAI GWGCWC 1 cut(s) 564
BsiSI CCGG 1 cut(s) 378
BslFI GGGAC 3 cut(s) 591, 643, 663
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 2 cut(s) 224, 667
BsmFI GGGAC 3 cut(s) 591, 643, 663
BsnI GGCC 2 cut(s) 176, 689
Bso31I GGTCTC 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 564
Bsp1407I TGTACA 1 cut(s) 445
Bsp143I GATC 5 cut(s) 38, 334, 440, 490, 661
BspACI CCGC 1 cut(s) 248
BspANI GGCC 2 cut(s) 176, 689
BspCNI CTCAG 1 cut(s) 220
BspHI TCATGA 1 cut(s) 96
BspLI GGNNCC 1 cut(s) 281
BspPI GGATC 3 cut(s) 46, 448, 498
BspTNI GGTCTC 1 cut(s) 224
BsrGI TGTACA 1 cut(s) 445
BsrI ACTGG 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 5 cut(s) 38, 334, 440, 490, 661
BssT1I CCWWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 94, 436
BstAUI TGTACA 1 cut(s) 445
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 1 cut(s) 228
BstF5I GGATG 5 cut(s) 123, 229, 310, 374, 592
BstKTI GATC 5 cut(s) 41, 337, 443, 493, 664
BstMAI GTCTC 2 cut(s) 224, 667
BstMBI GATC 5 cut(s) 38, 334, 440, 490, 661
BstMWI GCNNNNNNNGC 2 cut(s) 50, 257
BstNSI RCATGY 2 cut(s) 428, 452
BstV1I GCAGC 1 cut(s) 440
BstX2I RGATCY 2 cut(s) 440, 661
BstYI RGATCY 2 cut(s) 440, 661
BsuRI GGCC 2 cut(s) 176, 689
BtrI CACGTC 1 cut(s) 56
BtsCI GGATG 5 cut(s) 123, 229, 310, 374, 592
BtsIMutI CAGTG 1 cut(s) 441
Cac8I GCNNGC 1 cut(s) 426
CciI TCATGA 1 cut(s) 96
Csp6I GTAC 1 cut(s) 446
CviAII CATG 3 cut(s) 97, 425, 449
CviJI RGCY 8 cut(s) 8, 44, 71, 176, 266, 280, 541, 689
CviKI_1 RGCY 8 cut(s) 8, 44, 71, 176, 266, 280, 541, 689
CviQI GTAC 1 cut(s) 446
DdeI CTNAG 1 cut(s) 228
DpnI GATC 5 cut(s) 40, 336, 442, 492, 663
DpnII GATC 5 cut(s) 38, 334, 440, 490, 661
Eco130I CCWWGG 1 cut(s) 165
Eco31I GGTCTC 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 3 cut(s) 100, 428, 452
FaiI YATR 9 cut(s) 84, 98, 299, 351, 426, 450, 513, 584, 659
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FaqI GGGAC 3 cut(s) 591, 643, 663
FatI CATG 3 cut(s) 96, 424, 448
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 249, 429
FokI GGATG 5 cut(s) 110, 236, 317, 361, 579
Fsp4HI GCNGC 2 cut(s) 249, 429
FspBI CTAG 1 cut(s) 694
GluI GCNGC 2 cut(s) 249, 429
GsuI CTGGAG 1 cut(s) 438
HaeIII GGCC 2 cut(s) 176, 689
HapII CCGG 1 cut(s) 378
Hin1II CATG 3 cut(s) 100, 428, 452
HinfI GANTC 4 cut(s) 11, 361, 471, 579
HpaII CCGG 1 cut(s) 378
HphI GGTGA 1 cut(s) 302
Hpy188I TCNGA 2 cut(s) 128, 334
Hpy188III TCNNGA 5 cut(s) 25, 97, 215, 365, 417
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 2 cut(s) 31, 347
HpyCH4III ACNGT 2 cut(s) 94, 436
HpyCH4IV ACGT 1 cut(s) 55
HpyCH4V TGCA 2 cut(s) 199, 428
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 257
HpyF3I CTNAG 1 cut(s) 228
HpySE526I ACGT 1 cut(s) 55
Hsp92II CATG 3 cut(s) 100, 428, 452
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 5 cut(s) 38, 334, 440, 490, 661
LmnI GCTCC 1 cut(s) 285
LpnPI CCDG 6 cut(s) 153, 297, 391, 402, 534, 558
Lsp1109I GCAGC 1 cut(s) 440
LweI GCATC 3 cut(s) 169, 214, 295
MaeI CTAG 1 cut(s) 694
MaeII ACGT 1 cut(s) 55
MaeIII GTNAC 2 cut(s) 290, 451
MalI GATC 5 cut(s) 40, 336, 442, 492, 663
MboI GATC 5 cut(s) 38, 334, 440, 490, 661
MboII GAAGA 2 cut(s) 9, 398
MflI RGATCY 2 cut(s) 440, 661
MhlI GDGCHC 1 cut(s) 564
MluCI AATT 3 cut(s) 86, 324, 398
MlyI GAGTC 1 cut(s) 573
MnlI CCTC 6 cut(s) 136, 413, 541, 544, 606, 679
MseI TTAA 3 cut(s) 323, 462, 615
MslI CAYNNNNRTG 2 cut(s) 95, 239
MspI CCGG 1 cut(s) 378
MwoI GCNNNNNNNGC 2 cut(s) 50, 257
NdeII GATC 5 cut(s) 38, 334, 440, 490, 661
NlaIII CATG 3 cut(s) 100, 428, 452
NlaIV GGNNCC 1 cut(s) 281
NmuCI GTSAC 1 cut(s) 290
NspI RCATGY 2 cut(s) 428, 452
OliI CACNNNNGTG 1 cut(s) 239
PaeI GCATGC 1 cut(s) 428
PagI TCATGA 1 cut(s) 96
PciI ACATGT 1 cut(s) 448
PfeI GAWTC 3 cut(s) 11, 361, 471
PkrI GCNGC 2 cut(s) 250, 430
PleI GAGTC 1 cut(s) 573
PpsI GAGTC 1 cut(s) 573
PscI ACATGT 1 cut(s) 448
PshBI ATTAAT 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 281
PsuI RGATCY 2 cut(s) 440, 661
RsaI GTAC 1 cut(s) 447
RsaNI GTAC 1 cut(s) 446
RseI CAYNNNNRTG 2 cut(s) 95, 239
SaqAI TTAA 3 cut(s) 323, 462, 615
SatI GCNGC 2 cut(s) 249, 429
Sau3AI GATC 5 cut(s) 38, 334, 440, 490, 661
SchI GAGTC 1 cut(s) 573
SduI GDGCHC 1 cut(s) 564
SetI ASST 4 cut(s) 58, 358, 462, 543
SfaNI GCATC 3 cut(s) 169, 214, 295
SmiMI CAYNNNNRTG 2 cut(s) 95, 239
SphI GCATGC 1 cut(s) 428
Sse9I AATT 3 cut(s) 86, 324, 398
SsiI CCGC 1 cut(s) 248
SspI AATATT 1 cut(s) 66
SspMI CTAG 1 cut(s) 694
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 94, 436
TaiI ACGT 1 cut(s) 58
TaqI TCGA 3 cut(s) 47, 58, 493
TasI AATT 3 cut(s) 86, 324, 398
TatI WGTACW 1 cut(s) 445
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 3 cut(s) 11, 361, 471
Tru1I TTAA 3 cut(s) 323, 462, 615
Tru9I TTAA 3 cut(s) 323, 462, 615
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 1 cut(s) 428
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 3 cut(s) 99, 366, 540
TspGWI ACGGA 1 cut(s) 146
TspRI CASTG 1 cut(s) 441
VspI ATTAAT 1 cut(s) 323
XapI RAATTY 1 cut(s) 86
XceI RCATGY 2 cut(s) 428, 452
XspI CTAG 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.