MD08G1046500.v1.1

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003789
Physical Location & Seq
Reverse (-)
3515266 .. 3519846
4581 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1046500.v1.1.491

Sequence Viewer

Length: 1683 bp
ATGCCCGGTGACGAAAAATGGTGCGTGGTCACCGGAGGTCGAGGCTTCGCGGCGCGCCACTTGGTCCAGATGCTGATCCGATACGACGTGTTCCGGGTCCGAATCGCCGACCTGGGCTCCACCGTCGATCTAGAACCTTCCGAGGAGAACGGCATTCTCGCCGAAGCTCTGAGCTCCGGCCGCGCCCAGTACGTGTCCCTCGACCTTCGCAGCAAGAGCCAAGTTCTTCAAGTGTTTCAAGGTGCGGAGGTTGTGTTTCACATGGCGGCTCCCAATTCGTCCATCAACAATTACAAGCTCCACCACTCGGTCAATGTAGAAGGAACACAGAACGTGATTGATGCTTGTACTGAGCTGAAAGTAAAGAGACTAATCTATACTAGCTCCCCGAGTGTTGTTTTCGATGGGATTCATGGGATTGTTGACGGGGATGAGTCGTTGCCATATCCACCTAAGCACAATGATTTCTATTCGGCAACGAAAGCTCAGGGAGAAGAATTGGTGATCAAGGCAAATGGTGTTAAGGGGCTTCTCACATGTTGCATTCGCCCTAGCAGCATTTTTGGACCTGGTGATAGGTTGCTCGTTCCGTCTTTGGTAGTTGCTGCCAGGGCAGGGAAATCCAAGTACATCATTGGTGATGGCAACAACATATATGATTTCACATATGTTGAAAATGTGGCACATGCGCATATATGTGCAGAGCGAGCTCTTGCATCAGAGGGGACAGTTGCAGATAAAGCAGCGGGGCAGGCATATTTTATAACCAATATGGAACCTATCAAGTTTTGGGAGTTTGTTTCACTTATTCTAGAAGGTCTTGGCTATGAAAGGCCAAGAATAAAAATCCCTGCATTTGTTGTGATGCCAATTGCACATATGGTGGAGTTGACATATAAGCTGTTAGGCCCATATGGGATGAAGGTTCCACAGTTGACCCCTTCGAGAATCAGACTTCTCTCTTGCACCCGATCTTTTAAATGTTCGAAAGCACAGGATCGAATTGGCTACACACCCATCGTATCGCTTCAGGACGGTCTGAAAAGGACAATCGAGGCATACCCACATTTGAGGGCTGAACATCGACCGAAAAGAGGAGGACCATCTAAAGCGTCAATATATCTGGGAAGTGGAAGGGTTGCTGACACATTACTCTGGAAGGATAAAAAACAGACCTTTAAGGCGTTATTTGTTTTGATTGCAATCTACTACAGCTTCATTGTACCTGGATCTACAATTATTACTATGCTTTCAAGGGTTCTCCTGGTAGCTTCAGTATTCGTGTTCATTCATGCTGTTTTACCAGAAAAGATATTAGGATACAAAATTGAGAAGCTTCAGTCATCACATTTCCACTTATCAGAAGAGACGTCGCAACAAATTGCCAAGTCTGTGGCTTCATCATGGAATGCATCTGTTAAAACTCTGAAATCTCTTGGCCAGGGAGATGATGGGATATTGTTCCTTAAGGTTGCTCTCACCCTATTGGTTCTTAGCTTCCTTGGAGTGTTTTCACTTCAGAACTTATATGTTATAGGAGTTCCCGCTGCCTTTATAGTTTTCTACGTGTATGAGAAGGAGGAGCAAAAAATTGACGACATGGTCCTCAAAGCTCTCTCTCGTGGATGCAAATTGAAATCAGATATTGTCAAAAAATTCTGTACCTCCAAGAAGATTGACTAA

Protein Analysis

561

Amino Acids

61.85

Weight (kDa)

9.15

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 9 - 283 3.1e-76 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 9 - 237 6.8e-34 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 129 4.8e-08 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 10 - 238 4.7e-17 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 57 - 133 1.1e-07 NAD(P)H-binding
RmlD_sub_bind PF04321 60 - 281 7.7e-09 RmlD substrate binding domain
NAD_binding_4 PF07993 72 - 229 3.8e-13 Male sterility protein
Reticulon PF02453 380 - 537 5.1e-33 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 764
AasI GACNNNNNNGTC 1 cut(s) 1601
AatII GACGTC 1 cut(s) 1373
Acc16I TGCGCA 1 cut(s) 690
AccII CGCG 3 cut(s) 50, 55, 183
AciI CCGC 6 cut(s) 50, 181, 245, 266, 746, 1545
AclWI GGATC 3 cut(s) 70, 1005, 1237
AcoI YGGCCR 2 cut(s) 178, 1438
AcsI RAATTY 1 cut(s) 1655
AcuI CTGAAG 4 cut(s) 1013, 1257, 1322, 1502
AcyI GRCGYC 1 cut(s) 1370
AfaI GTAC 5 cut(s) 191, 349, 629, 1224, 1663
AfiI CCNNNNNNNGG 3 cut(s) 307, 615, 1094
AflII CTTAAG 1 cut(s) 1466
AflIII ACRYGT 4 cut(s) 87, 192, 536, 1566
AgsI TTSAA 5 cut(s) 230, 239, 674, 1254, 1636
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 6 cut(s) 111, 568, 608, 1225, 1263, 1440
Alw21I GWGCWC 2 cut(s) 176, 712
Alw26I GTCTC 2 cut(s) 361, 1361
AlwI GGATC 3 cut(s) 70, 1005, 1237
AlwNI CAGNNNCTG 1 cut(s) 73
Ama87I CYCGRG 1 cut(s) 388
AoxI GGCC 4 cut(s) 178, 833, 907, 1438
ApeKI GCWGC 5 cut(s) 210, 555, 605, 743, 1547
ApoI RAATTY 1 cut(s) 1655
AscI GGCGCGCC 1 cut(s) 53
AspLEI GCGC 4 cut(s) 55, 57, 185, 691
AspS9I GGNCC 6 cut(s) 64, 97, 566, 908, 1100, 1603
AsuC2I CCSGG 2 cut(s) 6, 95
AsuHPI GGTGA 6 cut(s) 20, 22, 514, 584, 650, 1471
AsuII TTCGAA 1 cut(s) 986
AvaI CYCGRG 1 cut(s) 388
AvaII GGWCC 5 cut(s) 64, 97, 566, 1100, 1603
BalI TGGCCA 1 cut(s) 1440
BanII GRGCYC 3 cut(s) 119, 176, 712
BauI CACGAG 1 cut(s) 1620
Bbv12I GWGCWC 2 cut(s) 176, 712
BbvI GCAGC 5 cut(s) 222, 567, 592, 755, 1534
BccI CCATC 6 cut(s) 290, 398, 635, 1025, 1111, 1445
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 6 cut(s) 113, 570, 610, 1227, 1265, 1442
BciVI GTATCC 1 cut(s) 1313
BclI TGATCA 1 cut(s) 504
BcnI CCSGG 2 cut(s) 6, 95
BcoDI GTCTC 2 cut(s) 361, 1361
BfaI CTAG 4 cut(s) 131, 381, 552, 812
BfmI CTRYAG 1 cut(s) 1210
BfrI CTTAAG 1 cut(s) 1466
BfuI GTATCC 1 cut(s) 1313
BisI GCNGC 8 cut(s) 51, 181, 211, 267, 556, 606, 744, 1548
BlsI GCNGC 8 cut(s) 52, 182, 212, 268, 557, 607, 745, 1549
Bme1390I CCNGG 8 cut(s) 6, 95, 113, 570, 610, 1227, 1265, 1442
Bme18I GGWCC 5 cut(s) 64, 97, 566, 1100, 1603
BmeT110I CYCGRG 1 cut(s) 388
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 6 cut(s) 64, 97, 566, 908, 1100, 1603
BmiI GGNNCC 5 cut(s) 98, 118, 270, 777, 927
BmrFI CCNGG 8 cut(s) 6, 95, 113, 570, 610, 1227, 1265, 1442
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 6 cut(s) 60, 331, 725, 855, 1421, 1616
BmuI ACTGGG 1 cut(s) 181
Bpu10I CCTNAGC 2 cut(s) 453, 486
Bpu14I TTCGAA 1 cut(s) 986
BpuMI CCSGG 2 cut(s) 6, 95
BsaAI YACGTR 2 cut(s) 193, 1567
BsaBI GATNNNNATC 2 cut(s) 74, 1202
BsaHI GRCGYC 1 cut(s) 1370
BsaJI CCNNGG 5 cut(s) 112, 141, 609, 1441, 1501
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 239, 269
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 615, 1094
Bse1I ACTGG 1 cut(s) 187
Bse8I GATNNNNATC 2 cut(s) 74, 1202
BseBI CCWGG 6 cut(s) 113, 570, 610, 1227, 1265, 1442
BseDI CCNNGG 5 cut(s) 112, 141, 609, 1441, 1501
BseGI GGATG 3 cut(s) 436, 924, 1631
BseJI GATNNNNATC 2 cut(s) 74, 1202
BseLI CCNNNNNNNGG 3 cut(s) 307, 615, 1094
BseMII CTCAG 3 cut(s) 161, 342, 500
BseNI ACTGG 1 cut(s) 187
BsePI GCGCGC 1 cut(s) 53
BseRI GAGGAG 3 cut(s) 158, 1110, 1595
BseX3I CGGCCG 1 cut(s) 178
BseXI GCAGC 5 cut(s) 222, 567, 592, 755, 1534
BsgI GTGCAG 1 cut(s) 720
Bsh1236I CGCG 3 cut(s) 50, 55, 183
Bsh1285I CGRYCG 2 cut(s) 181, 1088
BshFI GGCC 4 cut(s) 180, 835, 909, 1440
BsiEI CGRYCG 2 cut(s) 181, 1088
BsiHKAI GWGCWC 2 cut(s) 176, 712
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 4 cut(s) 6, 33, 94, 177
BslFI GGGAC 2 cut(s) 181, 739
BslI CCNNNNNNNGG 3 cut(s) 307, 615, 1094
BsmAI GTCTC 2 cut(s) 361, 1361
BsmBI CGTCTC 1 cut(s) 1361
BsmFI GGGAC 2 cut(s) 181, 739
BsmI GAATGC 3 cut(s) 153, 543, 1414
BsnI GGCC 4 cut(s) 180, 835, 909, 1440
BsoBI CYCGRG 1 cut(s) 388
Bsp119I TTCGAA 1 cut(s) 986
Bsp1286I GDGCHC 3 cut(s) 119, 176, 712
Bsp143I GATC 6 cut(s) 75, 127, 504, 971, 997, 1229
BspACI CCGC 6 cut(s) 50, 181, 245, 266, 746, 1545
BspANI GGCC 4 cut(s) 180, 835, 909, 1440
BspCNI CTCAG 3 cut(s) 162, 343, 499
BspFNI CGCG 3 cut(s) 50, 55, 183
BspLI GGNNCC 5 cut(s) 98, 118, 270, 777, 927
BspPI GGATC 3 cut(s) 70, 1005, 1237
BspT104I TTCGAA 1 cut(s) 986
BspTI CTTAAG 1 cut(s) 1466
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 5 cut(s) 112, 141, 609, 1441, 1501
BssHII GCGCGC 1 cut(s) 53
BssMI GATC 6 cut(s) 75, 127, 504, 971, 997, 1229
BssNI GRCGYC 1 cut(s) 1370
BssSI CACGAG 1 cut(s) 1620
BssT1I CCWWGG 1 cut(s) 1501
Bst2BI CACGAG 1 cut(s) 1620
Bst2UI CCWGG 6 cut(s) 113, 570, 610, 1227, 1265, 1442
Bst4CI ACNGT 4 cut(s) 124, 730, 933, 1037
Bst6I CTCTTC 1 cut(s) 1359
BstACI GRCGYC 1 cut(s) 1370
BstAFI CTTAAG 1 cut(s) 1466
BstBAI YACGTR 2 cut(s) 193, 1567
BstBI TTCGAA 1 cut(s) 986
BstC8I GCNNGC 3 cut(s) 55, 708, 753
BstDEI CTNAG 5 cut(s) 170, 351, 453, 486, 1493
BstEII GGTNACC 1 cut(s) 28
BstF5I GGATG 3 cut(s) 436, 924, 1631
BstFNI CGCG 3 cut(s) 50, 55, 183
BstHHI GCGC 4 cut(s) 55, 57, 185, 691
BstKTI GATC 6 cut(s) 78, 130, 507, 974, 1000, 1232
BstMAI GTCTC 2 cut(s) 361, 1361
BstMBI GATC 6 cut(s) 75, 127, 504, 971, 997, 1229
BstMCI CGRYCG 2 cut(s) 181, 1088
BstMWI GCNNNNNNNGC 8 cut(s) 180, 216, 482, 555, 611, 707, 740, 752
BstNI CCWGG 6 cut(s) 113, 570, 610, 1227, 1265, 1442
BstNSI RCATGY 2 cut(s) 540, 689
BstPI GGTNACC 1 cut(s) 28
BstSCI CCNGG 8 cut(s) 4, 93, 111, 568, 608, 1225, 1263, 1440
BstSFI CTRYAG 1 cut(s) 1210
BstUI CGCG 3 cut(s) 50, 55, 183
BstV1I GCAGC 5 cut(s) 222, 567, 592, 755, 1534
BstX2I RGATCY 1 cut(s) 1229
BstXI CCANNNNNNTGG 1 cut(s) 1393
BstYI RGATCY 1 cut(s) 1229
BstZI CGGCCG 1 cut(s) 178
BsuI GTATCC 1 cut(s) 1313
BsuRI GGCC 4 cut(s) 180, 835, 909, 1440
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 3 cut(s) 436, 924, 1631
Cac8I GCNNGC 3 cut(s) 55, 708, 753
CaiI CAGNNNCTG 1 cut(s) 73
CfoI GCGC 4 cut(s) 55, 57, 185, 691
Cfr13I GGNCC 6 cut(s) 64, 97, 566, 908, 1100, 1603
CseI GACGC 1 cut(s) 1101
CsiI ACCWGGT 1 cut(s) 568
Csp6I GTAC 5 cut(s) 190, 348, 628, 1223, 1662
CviAII CATG 7 cut(s) 262, 413, 537, 686, 1292, 1404, 1600
CviQI GTAC 5 cut(s) 190, 348, 628, 1223, 1662
DdeI CTNAG 5 cut(s) 170, 351, 453, 486, 1493
DpnI GATC 6 cut(s) 77, 129, 506, 973, 999, 1231
DpnII GATC 6 cut(s) 75, 127, 504, 971, 997, 1229
DraI TTTAAA 1 cut(s) 979
DrdI GACNNNNNNGTC 1 cut(s) 1601
DseDI GACNNNNNNGTC 1 cut(s) 1601
EaeI YGGCCR 2 cut(s) 178, 1438
EagI CGGCCG 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 1359
EarI CTCTTC 1 cut(s) 1359
Ecl136II GAGCTC 2 cut(s) 174, 710
EclXI CGGCCG 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 1501
Eco24I GRGCYC 3 cut(s) 119, 176, 712
Eco47I GGWCC 5 cut(s) 64, 97, 566, 1100, 1603
Eco52I CGGCCG 1 cut(s) 178
Eco53kI GAGCTC 2 cut(s) 174, 710
Eco57I CTGAAG 4 cut(s) 1013, 1257, 1322, 1502
Eco88I CYCGRG 1 cut(s) 388
Eco91I GGTNACC 1 cut(s) 28
EcoICRI GAGCTC 2 cut(s) 174, 710
EcoO65I GGTNACC 1 cut(s) 28
EcoRII CCWGG 6 cut(s) 111, 568, 608, 1225, 1263, 1440
EcoT14I CCWWGG 1 cut(s) 1501
EcoT22I ATGCAT 1 cut(s) 1414
EcoT38I GRGCYC 3 cut(s) 119, 176, 712
ErhI CCWWGG 1 cut(s) 1501
Esp3I CGTCTC 1 cut(s) 1361
FaeI CATG 7 cut(s) 265, 416, 540, 689, 1295, 1407, 1603
FaqI GGGAC 2 cut(s) 181, 739
FatI CATG 7 cut(s) 261, 412, 536, 685, 1291, 1403, 1599
FauI CCCGC 2 cut(s) 739, 1552
FauNDI CATATG 3 cut(s) 667, 879, 913
FbaI TGATCA 1 cut(s) 504
Fnu4HI GCNGC 8 cut(s) 51, 181, 211, 267, 556, 606, 744, 1548
FokI GGATG 3 cut(s) 443, 931, 1638
FriOI GRGCYC 3 cut(s) 119, 176, 712
Fsp4HI GCNGC 8 cut(s) 51, 181, 211, 267, 556, 606, 744, 1548
FspAI RTGCGCAY 1 cut(s) 690
FspBI CTAG 4 cut(s) 131, 381, 552, 812
FspI TGCGCA 1 cut(s) 690
GlaI GCGC 4 cut(s) 54, 56, 184, 690
GluI GCNGC 8 cut(s) 51, 181, 211, 267, 556, 606, 744, 1548
HaeIII GGCC 4 cut(s) 180, 835, 909, 1440
HapII CCGG 4 cut(s) 6, 33, 94, 177
HgaI GACGC 1 cut(s) 1101
HhaI GCGC 4 cut(s) 55, 57, 185, 691
Hin1I GRCGYC 1 cut(s) 1370
Hin1II CATG 7 cut(s) 265, 416, 540, 689, 1295, 1407, 1603
Hin6I GCGC 4 cut(s) 53, 55, 183, 689
HinP1I GCGC 4 cut(s) 53, 55, 183, 689
HincII GTYRAC 3 cut(s) 424, 891, 936
HindII GTYRAC 3 cut(s) 424, 891, 936
HindIII AAGCTT 1 cut(s) 1334
HinfI GANTC 4 cut(s) 102, 409, 434, 948
HpaII CCGG 4 cut(s) 6, 33, 94, 177
HphI GGTGA 6 cut(s) 20, 22, 514, 584, 650, 1471
Hpy166II GTNNAC 3 cut(s) 424, 891, 936
Hpy188III TCNNGA 6 cut(s) 67, 131, 812, 945, 1031, 1156
Hpy8I GTNNAC 3 cut(s) 424, 891, 936
Hpy99I CGWCG 3 cut(s) 89, 128, 1375
HpyAV CCTTC 9 cut(s) 147, 215, 314, 809, 916, 951, 1128, 1153, 1570
HpyCH4III ACNGT 4 cut(s) 124, 730, 933, 1037
HpyCH4IV ACGT 5 cut(s) 87, 192, 333, 1370, 1566
HpyF10VI GCNNNNNNNGC 8 cut(s) 180, 216, 482, 555, 611, 707, 740, 752
HpyF3I CTNAG 5 cut(s) 170, 351, 453, 486, 1493
HpySE526I ACGT 5 cut(s) 87, 192, 333, 1370, 1566
Hsp92I GRCGYC 1 cut(s) 1370
Hsp92II CATG 7 cut(s) 265, 416, 540, 689, 1295, 1407, 1603
HspAI GCGC 4 cut(s) 53, 55, 183, 689
Ksp22I TGATCA 1 cut(s) 504
Kzo9I GATC 6 cut(s) 75, 127, 504, 971, 997, 1229
LmnI GCTCC 6 cut(s) 122, 179, 274, 303, 389, 1582
Lsp1109I GCAGC 5 cut(s) 222, 567, 592, 755, 1534
LweI GCATC 6 cut(s) 60, 331, 725, 855, 1421, 1616
MabI ACCWGGT 1 cut(s) 568
MaeI CTAG 4 cut(s) 131, 381, 552, 812
MaeII ACGT 5 cut(s) 87, 192, 333, 1370, 1566
MaeIII GTNAC 2 cut(s) 8, 28
MalI GATC 6 cut(s) 77, 129, 506, 973, 999, 1231
MboI GATC 6 cut(s) 75, 127, 504, 971, 997, 1229
MboII GAAGA 3 cut(s) 218, 506, 1376
MfeI CAATTG 1 cut(s) 870
MflI RGATCY 1 cut(s) 1229
MhlI GDGCHC 3 cut(s) 119, 176, 712
MlsI TGGCCA 1 cut(s) 1440
MluNI TGGCCA 1 cut(s) 1440
MlyI GAGTC 1 cut(s) 443
Mox20I TGGCCA 1 cut(s) 1440
Mph1103I ATGCAT 1 cut(s) 1414
MscI TGGCCA 1 cut(s) 1440
MseI TTAA 5 cut(s) 522, 978, 1179, 1419, 1467
MslI CAYNNNNRTG 1 cut(s) 696
Msp20I TGGCCA 1 cut(s) 1440
MspA1I CMGCKG 2 cut(s) 746, 1547
MspCI CTTAAG 1 cut(s) 1466
MspI CCGG 4 cut(s) 6, 33, 94, 177
MspR9I CCNGG 8 cut(s) 6, 95, 113, 570, 610, 1227, 1265, 1442
MunI CAATTG 1 cut(s) 870
Mva1269I GAATGC 3 cut(s) 153, 543, 1414
MvaI CCWGG 6 cut(s) 113, 570, 610, 1227, 1265, 1442
MvnI CGCG 3 cut(s) 50, 55, 183
MwoI GCNNNNNNNGC 8 cut(s) 180, 216, 482, 555, 611, 707, 740, 752
NciI CCSGG 2 cut(s) 6, 95
NdeI CATATG 3 cut(s) 667, 879, 913
NdeII GATC 6 cut(s) 75, 127, 504, 971, 997, 1229
NlaIII CATG 7 cut(s) 265, 416, 540, 689, 1295, 1407, 1603
NlaIV GGNNCC 5 cut(s) 98, 118, 270, 777, 927
NmuCI GTSAC 2 cut(s) 8, 28
NsbI TGCGCA 1 cut(s) 690
NsiI ATGCAT 1 cut(s) 1414
NspI RCATGY 2 cut(s) 540, 689
NspV TTCGAA 1 cut(s) 986
PalAI GGCGCGCC 1 cut(s) 53
PauI GCGCGC 1 cut(s) 53
PciI ACATGT 1 cut(s) 536
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 3 cut(s) 153, 543, 1414
PfeI GAWTC 3 cut(s) 102, 409, 948
PflFI GACNNNGTC 1 cut(s) 1601
PkrI GCNGC 8 cut(s) 52, 182, 212, 268, 557, 607, 745, 1549
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
Ppu21I YACGTR 2 cut(s) 193, 1567
PscI ACATGT 1 cut(s) 536
PsiI TTATAA 1 cut(s) 764
Psp124BI GAGCTC 2 cut(s) 176, 712
Psp6I CCWGG 6 cut(s) 111, 568, 608, 1225, 1263, 1440
PspEI GGTNACC 1 cut(s) 28
PspGI CCWGG 6 cut(s) 111, 568, 608, 1225, 1263, 1440
PspN4I GGNNCC 5 cut(s) 98, 118, 270, 777, 927
PspPI GGNCC 6 cut(s) 64, 97, 566, 908, 1100, 1603
PstNI CAGNNNCTG 1 cut(s) 73
PsuI RGATCY 1 cut(s) 1229
PsyI GACNNNGTC 1 cut(s) 1601
PteI GCGCGC 1 cut(s) 53
RsaI GTAC 5 cut(s) 191, 349, 629, 1224, 1663
RsaNI GTAC 5 cut(s) 190, 348, 628, 1223, 1662
RseI CAYNNNNRTG 1 cut(s) 696
SacI GAGCTC 2 cut(s) 176, 712
SaqAI TTAA 5 cut(s) 522, 978, 1179, 1419, 1467
SatI GCNGC 8 cut(s) 51, 181, 211, 267, 556, 606, 744, 1548
Sau3AI GATC 6 cut(s) 75, 127, 504, 971, 997, 1229
Sau96I GGNCC 6 cut(s) 64, 97, 566, 908, 1100, 1603
SchI GAGTC 1 cut(s) 443
ScrFI CCNGG 8 cut(s) 6, 95, 113, 570, 610, 1227, 1265, 1442
SduI GDGCHC 3 cut(s) 119, 176, 712
SexAI ACCWGGT 1 cut(s) 568
SfaNI GCATC 6 cut(s) 60, 331, 725, 855, 1421, 1616
SfcI CTRYAG 1 cut(s) 1210
SfuI TTCGAA 1 cut(s) 986
SgsI GGCGCGCC 1 cut(s) 53
SinI GGWCC 5 cut(s) 64, 97, 566, 1100, 1603
SmiMI CAYNNNNRTG 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 1466
SmoI CTYRAG 1 cut(s) 1466
SsiI CCGC 6 cut(s) 50, 181, 245, 266, 746, 1545
SspMI CTAG 4 cut(s) 131, 381, 552, 812
SstI GAGCTC 2 cut(s) 176, 712
StyD4I CCNGG 8 cut(s) 4, 93, 111, 568, 608, 1225, 1263, 1440
StyI CCWWGG 1 cut(s) 1501
TaaI ACNGT 4 cut(s) 124, 730, 933, 1037
TaiI ACGT 5 cut(s) 90, 195, 336, 1373, 1569
TaqI TCGA 9 cut(s) 40, 126, 201, 402, 944, 986, 1000, 1053, 1084
TaqII GACCGA 2 cut(s) 298, 1102
TatI WGTACW 2 cut(s) 347, 627
TauI GCSGC 3 cut(s) 53, 183, 269
TfiI GAWTC 3 cut(s) 102, 409, 948
Tru1I TTAA 5 cut(s) 522, 978, 1179, 1419, 1467
Tru9I TTAA 5 cut(s) 522, 978, 1179, 1419, 1467
TseFI GTSAC 2 cut(s) 8, 28
TseI GCWGC 5 cut(s) 210, 555, 605, 743, 1547
Tsp45I GTSAC 2 cut(s) 8, 28
TspDTI ATGAA 7 cut(s) 401, 843, 935, 1207, 1276, 1280, 1389
TspGWI ACGGA 1 cut(s) 579
Tth111I GACNNNGTC 1 cut(s) 1601
Vha464I CTTAAG 1 cut(s) 1466
VpaK11BI GGWCC 5 cut(s) 64, 97, 566, 1100, 1603
XapI RAATTY 1 cut(s) 1655
XbaI TCTAGA 2 cut(s) 130, 811
XceI RCATGY 2 cut(s) 540, 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 1448
XspI CTAG 4 cut(s) 131, 381, 552, 812
ZraI GACGTC 1 cut(s) 1371
Zsp2I ATGCAT 1 cut(s) 1414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.