Rorug06G0334700

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003789
Physical Location & Seq
Forward (+)
48722465 .. 48727206
4742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0334700.1

Sequence Viewer

Length: 1620 bp
ATGGCGATGGCGGTTGGGCCAGCTGCGTTGCCGCCGGCCGATGGAGGCAGTCCTCCAGCGATGGGACCCGCAATGATGCCGATGACGGTGGTGCCGGACGCGGTGGCGAAGGATGCAATAATTGGGTGGTACCGCGGCGAGTTTGCGGCCGCCAACGCGATCATTGACGCACTCTGCGGGCACCTCACACAGCTCAGCGGCGGAGGAGGGGCCGACTACGGCGCCGTGTTCGCCGCGATACACCGCAGGCGGATGAATTGGATCCCGGTGCTCCAGATGCAGAAGTACCACTCCATCGCTGACGTGATGGTGGAGCTCCGAAACCTCGGGGCGAAGATGGCGGAGCGGGAGAGCCACCACCAAAACGGGGACTTTTTTGAGGCCGAGGACAATTGCAAGAGCAGCGAGGGGGCTAAGTCGTGTGTGGAGGACGAGAAGGTGGAAGAAACTAATGGAAATGAGCCGGCCGCCGATGAGGTCACTGAAGAAGACGGTTCGCCGGAGAGTGATATTACCGACTCAGGATCTCAAGAAGTGCCCAATTCAGTGAACACTGAGATATGTTCTAACCATGATGACTGTGAGGCACGTCGTGCCCAGGTCAAGTTGACAAAAGGTTTCTCAGCAAAGGAATCAGTCAAAGGGCATATGGTTAATGTTGTGAAAGGCTTAAAGCTATATGAGGAAATATTTACTGACTCTGAGCTCTCTAAATTGACTGACTTTGCAAACGAACTCCGCACGGCTGGTCTGAATGGAGAGCTCTCAGGTGAGACCTTTATTTTGTTCAACAAGCAGATTAAGGGAAACAGGAGAGAGCTGATTCAGTTTGGAATTCCAATATTTGGACAGGTCAAAGATGAAGCTGCAAGCAACATAGGGCCAATTCCAGCTCTTCTCCATGATGTGATTGATCACCTTGTACTGTGGCAACTAATTCCAGAGTATAAAAGACCTAATGGTTGCATTATCAACTTCTTTGAGGAGGGAGAATATTCGCAGCCATTCCTGAAACCACCACATTTGGATCAGCCCATCTCCACTCTTCTCCTCTCTGAATCCACAATGGCTTTCGGGCGCACCCTTGTGACTGATACTGAAGGGAACTACAAGGGGCCGCTGACCCTCTCCTTGAAGGAAGGGTCTCTCCTAGTTATGAGGGGAAACAGTGCTGACATGGCAAGGCACGTCATGTGTCCATCTCCGAACAAGAGGATCAGCATCACATTCTTTAGAGTCCGAGTAGACTCCAACCAATTTCAGTCATCACCACCAAGTCCAACACCGAATGGTGCCATGACTGTATGGCAACATGGCGTTATGAGCCCATATGCAGCTCCAGATGGAGCACCCAATGGCTATGAGGCAATGGATGTAATGCCAAAATGGGGTGTCCTTCGTGCTCCGGTGGTCATGTTAGCCCCAGTTCGTCCTATGGTAGTGAGCCCTCGAAAAGTTCCTCAAAGTGGTACTGGAGTTTTCTTGCCATGGACTGTGGGATCAAGAAAACCAGCAAAACATCTTCCACCTCGCGCCCAAAAAGGACGGCTTATGGCACTCCCTTCCCCGGCCGAGACACATGTAGTGGAGTCCACTTCTGAGCCAGGCATCACTGTTTAA

Protein Analysis

539

Amino Acids

58.44

Weight (kDa)

5.47

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 322 - 412 1.7e-06 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 129
AccB1I GGYRCC 5 cut(s) 91, 129, 180, 221, 1292
AccB7I CCANNNNNTGG 1 cut(s) 845
AccBSI CCGCTC 1 cut(s) 346
AccI GTMKAC 1 cut(s) 1245
AccII CGCG 5 cut(s) 101, 135, 158, 236, 1533
AclWI GGATC 6 cut(s) 256, 269, 532, 1035, 1223, 1507
AcoI YGGCCR 4 cut(s) 36, 147, 465, 1569
AcsI RAATTY 1 cut(s) 834
AcuI CTGAAG 2 cut(s) 504, 1119
AcyI GRCGYC 1 cut(s) 222
AdeI CACNNNGTG 1 cut(s) 593
AfaI GTAC 4 cut(s) 131, 287, 924, 1471
AfiI CCNNNNNNNGG 7 cut(s) 41, 62, 367, 845, 1388, 1466, 1567
AflIII ACRYGT 1 cut(s) 1579
AgsI TTSAA 2 cut(s) 790, 1135
AjiI CACGTC 3 cut(s) 304, 590, 1189
AjnI CCWGG 2 cut(s) 597, 1603
AleI CACNNNNGTG 1 cut(s) 1085
Alw21I GWGCWC 6 cut(s) 273, 318, 708, 765, 1351, 1405
Alw26I GTCTC 3 cut(s) 767, 1149, 1568
AlwI GGATC 6 cut(s) 256, 269, 532, 1035, 1223, 1507
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 9 cut(s) 17, 36, 147, 210, 381, 465, 881, 1115, 1569
ApeKI GCWGC 5 cut(s) 23, 402, 866, 1000, 1334
ApoI RAATTY 1 cut(s) 834
ArsI GACNNNNNNTTYG 2 cut(s) 766, 798
Asp718I GGTACC 1 cut(s) 129
AspLEI GCGC 3 cut(s) 224, 1080, 1535
AspS9I GGNCC 5 cut(s) 17, 65, 210, 881, 1115
AsuC2I CCSGG 2 cut(s) 266, 1568
AsuHPI GGTGA 3 cut(s) 782, 908, 1260
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 1 cut(s) 65
BaeGI GKGCMC 3 cut(s) 183, 540, 598
BaeI ACNNNNGTAYC 2 cut(s) 1461, 1494
BamHI GGATCC 1 cut(s) 261
BanI GGYRCC 5 cut(s) 91, 129, 180, 221, 1292
BanII GRGCYC 5 cut(s) 318, 708, 765, 1328, 1448
BbsI GAAGAC 1 cut(s) 495
Bbv12I GWGCWC 6 cut(s) 273, 318, 708, 765, 1351, 1405
BbvI GCAGC 5 cut(s) 10, 414, 853, 1012, 1346
BccI CCATC 8 cut(s) 35, 55, 301, 302, 331, 1043, 1207, 1337
BceAI ACGGC 4 cut(s) 209, 235, 759, 1562
BciT130I CCWGG 2 cut(s) 599, 1605
BclI TGATCA 1 cut(s) 913
BcnI CCSGG 2 cut(s) 266, 1568
BcoDI GTCTC 3 cut(s) 767, 1149, 1568
BfaI CTAG 1 cut(s) 1151
BfoI RGCGCY 1 cut(s) 225
BlpI GCTNAGC 1 cut(s) 194
Bme1390I CCNGG 4 cut(s) 266, 599, 1568, 1605
Bme18I GGWCC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 326
BmgBI CACGTC 3 cut(s) 304, 590, 1189
BmgT120I GGNCC 5 cut(s) 17, 65, 210, 881, 1115
BmrFI CCNGG 4 cut(s) 266, 599, 1568, 1605
BmrI ACTGGG 1 cut(s) 1418
BmsI GCATC 4 cut(s) 66, 103, 267, 1230
BmuI ACTGGG 1 cut(s) 1418
BpiI GAAGAC 1 cut(s) 495
BpmI CTGGAG 4 cut(s) 39, 257, 1323, 1494
Bpu1102I GCTNAGC 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 513
BpuMI CCSGG 2 cut(s) 266, 1568
BsaBI GATNNNNATC 1 cut(s) 1220
BsaHI GRCGYC 1 cut(s) 222
BsaI GGTCTC 2 cut(s) 767, 1149
BsaJI CCNNGG 6 cut(s) 133, 325, 384, 597, 1487, 1566
BsaWI WCCGGW 1 cut(s) 1405
Bsc4I CCNNNNNNNGG 7 cut(s) 41, 62, 367, 845, 1388, 1466, 1567
Bse118I RCCGGY 2 cut(s) 34, 463
Bse1I ACTGG 2 cut(s) 1424, 1477
Bse3DI GCAATG 2 cut(s) 78, 1374
Bse8I GATNNNNATC 1 cut(s) 1220
BseBI CCWGG 2 cut(s) 599, 1605
BseDI CCNNGG 6 cut(s) 133, 325, 384, 597, 1487, 1566
BseGI GGATG 3 cut(s) 118, 258, 1378
BseJI GATNNNNATC 1 cut(s) 1220
BseLI CCNNNNNNNGG 7 cut(s) 41, 62, 367, 845, 1388, 1466, 1567
BseMI GCAATG 2 cut(s) 78, 1374
BseMII CTCAG 7 cut(s) 208, 534, 546, 636, 693, 780, 1590
BseNI ACTGG 2 cut(s) 1424, 1477
BseRI GAGGAG 3 cut(s) 219, 998, 1040
BseSI GKGCMC 3 cut(s) 183, 540, 598
BseX3I CGGCCG 4 cut(s) 36, 147, 465, 1569
BseXI GCAGC 5 cut(s) 10, 414, 853, 1012, 1346
Bsh1236I CGCG 5 cut(s) 101, 135, 158, 236, 1533
Bsh1285I CGRYCG 4 cut(s) 39, 150, 468, 1572
BshFI GGCC 9 cut(s) 19, 38, 149, 212, 383, 467, 883, 1117, 1571
BshNI GGYRCC 5 cut(s) 91, 129, 180, 221, 1292
BsiEI CGRYCG 4 cut(s) 39, 150, 468, 1572
BsiHKAI GWGCWC 6 cut(s) 273, 318, 708, 765, 1351, 1405
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 7 cut(s) 35, 95, 266, 464, 500, 1406, 1568
BslFI GGGAC 2 cut(s) 78, 383
BslI CCNNNNNNNGG 7 cut(s) 41, 62, 367, 845, 1388, 1466, 1567
BsmAI GTCTC 3 cut(s) 767, 1149, 1568
BsmFI GGGAC 2 cut(s) 78, 383
BsnI GGCC 9 cut(s) 19, 38, 149, 212, 383, 467, 883, 1117, 1571
Bso31I GGTCTC 2 cut(s) 767, 1149
BsoBI CYCGRG 1 cut(s) 326
Bsp143I GATC 7 cut(s) 159, 261, 524, 913, 1027, 1215, 1499
Bsp1720I GCTNAGC 1 cut(s) 194
Bsp19I CCATGG 1 cut(s) 1487
BspANI GGCC 9 cut(s) 19, 38, 149, 212, 383, 467, 883, 1117, 1571
BspCNI CTCAG 7 cut(s) 207, 533, 547, 635, 694, 779, 1591
BspFNI CGCG 5 cut(s) 101, 135, 158, 236, 1533
BspPI GGATC 6 cut(s) 256, 269, 532, 1035, 1223, 1507
BspQI GCTCTTC 1 cut(s) 900
BspT107I GGYRCC 5 cut(s) 91, 129, 180, 221, 1292
BspTNI GGTCTC 2 cut(s) 767, 1149
BsrBI CCGCTC 1 cut(s) 346
BsrDI GCAATG 2 cut(s) 78, 1374
BsrFI RCCGGY 2 cut(s) 34, 463
BsrI ACTGG 2 cut(s) 1424, 1477
BssAI RCCGGY 2 cut(s) 34, 463
BssECI CCNNGG 6 cut(s) 133, 325, 384, 597, 1487, 1566
BssMI GATC 7 cut(s) 159, 261, 524, 913, 1027, 1215, 1499
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 1487
Bst2UI CCWGG 2 cut(s) 599, 1605
Bst4CI ACNGT 8 cut(s) 88, 494, 581, 927, 1169, 1303, 1495, 1615
Bst6I CTCTTC 2 cut(s) 900, 1050
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 1 cut(s) 593
BstC8I GCNNGC 6 cut(s) 21, 36, 179, 248, 465, 871
BstDEI CTNAG 8 cut(s) 194, 414, 520, 555, 622, 702, 766, 1599
BstDSI CCRYGG 2 cut(s) 133, 1487
BstF5I GGATG 3 cut(s) 118, 258, 1378
BstFNI CGCG 5 cut(s) 101, 135, 158, 236, 1533
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 3 cut(s) 224, 1080, 1535
BstKTI GATC 7 cut(s) 162, 264, 527, 916, 1030, 1218, 1502
BstMAI GTCTC 3 cut(s) 767, 1149, 1568
BstMBI GATC 7 cut(s) 159, 261, 524, 913, 1027, 1215, 1499
BstMCI CGRYCG 4 cut(s) 39, 150, 468, 1572
BstMWI GCNNNNNNNGC 9 cut(s) 113, 155, 230, 277, 338, 402, 593, 1178, 1323
BstNI CCWGG 2 cut(s) 599, 1605
BstNSI RCATGY 1 cut(s) 1583
BstSCI CCNGG 4 cut(s) 264, 597, 1566, 1603
BstSLI GKGCMC 3 cut(s) 183, 540, 598
BstUI CGCG 5 cut(s) 101, 135, 158, 236, 1533
BstV1I GCAGC 5 cut(s) 10, 414, 853, 1012, 1346
BstV2I GAAGAC 1 cut(s) 495
BstX2I RGATCY 2 cut(s) 261, 524
BstYI RGATCY 2 cut(s) 261, 524
BstZI CGGCCG 4 cut(s) 36, 147, 465, 1569
BsuRI GGCC 9 cut(s) 19, 38, 149, 212, 383, 467, 883, 1117, 1571
BtgI CCRYGG 2 cut(s) 133, 1487
BtgZI GCGATG 3 cut(s) 20, 74, 280
BtrI CACGTC 3 cut(s) 304, 590, 1189
BtsCI GGATG 3 cut(s) 118, 258, 1378
BtsIMutI CAGTG 5 cut(s) 480, 552, 552, 1174, 1611
Cac8I GCNNGC 6 cut(s) 21, 36, 179, 248, 465, 871
CciNI GCGGCCGC 1 cut(s) 147
CfoI GCGC 3 cut(s) 224, 1080, 1535
Cfr10I RCCGGY 2 cut(s) 34, 463
Cfr13I GGNCC 5 cut(s) 17, 65, 210, 881, 1115
Cfr42I CCGCGG 1 cut(s) 136
CseI GACGC 2 cut(s) 107, 176
Csp6I GTAC 4 cut(s) 130, 286, 923, 1470
CspCI CAANNNNNGTGG 2 cut(s) 1005, 1040
CviAII CATG 9 cut(s) 572, 902, 1177, 1192, 1297, 1313, 1414, 1488, 1580
CviQI GTAC 4 cut(s) 130, 286, 923, 1470
DdeI CTNAG 8 cut(s) 194, 414, 520, 555, 622, 702, 766, 1599
DinI GGCGCC 1 cut(s) 223
DpnI GATC 7 cut(s) 161, 263, 526, 915, 1029, 1217, 1501
DpnII GATC 7 cut(s) 159, 261, 524, 913, 1027, 1215, 1499
DraIII CACNNNGTG 1 cut(s) 593
EaeI YGGCCR 4 cut(s) 36, 147, 465, 1569
EagI CGGCCG 4 cut(s) 36, 147, 465, 1569
Eam1104I CTCTTC 2 cut(s) 900, 1050
EarI CTCTTC 2 cut(s) 900, 1050
EciI GGCGGA 3 cut(s) 216, 265, 356
Ecl136II GAGCTC 3 cut(s) 316, 706, 763
EclXI CGGCCG 4 cut(s) 36, 147, 465, 1569
Eco130I CCWWGG 1 cut(s) 1487
Eco24I GRGCYC 5 cut(s) 318, 708, 765, 1328, 1448
Eco31I GGTCTC 2 cut(s) 767, 1149
Eco47I GGWCC 1 cut(s) 65
Eco52I CGGCCG 4 cut(s) 36, 147, 465, 1569
Eco53kI GAGCTC 3 cut(s) 316, 706, 763
Eco57I CTGAAG 2 cut(s) 504, 1119
Eco88I CYCGRG 1 cut(s) 326
EcoICRI GAGCTC 3 cut(s) 316, 706, 763
EcoO109I RGGNCCY 1 cut(s) 65
EcoRI GAATTC 1 cut(s) 834
EcoRII CCWGG 2 cut(s) 597, 1603
EcoT14I CCWWGG 1 cut(s) 1487
EcoT38I GRGCYC 5 cut(s) 318, 708, 765, 1328, 1448
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 1487
FaeI CATG 9 cut(s) 575, 905, 1180, 1195, 1300, 1316, 1417, 1491, 1583
FalI AAGNNNNNCTT 2 cut(s) 1533, 1565
FaqI GGGAC 2 cut(s) 78, 383
FatI CATG 9 cut(s) 571, 901, 1176, 1191, 1296, 1312, 1413, 1487, 1579
FauI CCCGC 3 cut(s) 76, 170, 339
FauNDI CATATG 2 cut(s) 648, 1330
FbaI TGATCA 1 cut(s) 913
FblI GTMKAC 1 cut(s) 1245
FokI GGATG 3 cut(s) 125, 265, 1385
FriOI GRGCYC 5 cut(s) 318, 708, 765, 1328, 1448
FspBI CTAG 1 cut(s) 1151
GlaI GCGC 3 cut(s) 223, 1079, 1534
GsuI CTGGAG 4 cut(s) 39, 257, 1323, 1494
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 9 cut(s) 19, 38, 149, 212, 383, 467, 883, 1117, 1571
HapII CCGG 7 cut(s) 35, 95, 266, 464, 500, 1406, 1568
HgaI GACGC 2 cut(s) 107, 176
HhaI GCGC 3 cut(s) 224, 1080, 1535
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 9 cut(s) 575, 905, 1180, 1195, 1300, 1316, 1417, 1491, 1583
Hin6I GCGC 3 cut(s) 222, 1078, 1533
HinP1I GCGC 3 cut(s) 222, 1078, 1533
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HinfI GANTC 8 cut(s) 518, 632, 698, 823, 1058, 1236, 1247, 1589
HpaII CCGG 7 cut(s) 35, 95, 266, 464, 500, 1406, 1568
HphI GGTGA 3 cut(s) 782, 908, 1260
Hpy166II GTNNAC 4 cut(s) 550, 609, 1246, 1593
Hpy188I TCNGA 7 cut(s) 320, 703, 753, 1057, 1206, 1241, 1600
Hpy188III TCNNGA 7 cut(s) 274, 522, 530, 941, 1009, 1340, 1503
Hpy8I GTNNAC 4 cut(s) 550, 609, 1246, 1593
Hpy99I CGWCG 1 cut(s) 594
HpyAV CCTTC 7 cut(s) 103, 430, 1094, 1129, 1133, 1406, 1572
HpyCH4III ACNGT 8 cut(s) 88, 494, 581, 927, 1169, 1303, 1495, 1615
HpyCH4IV ACGT 3 cut(s) 303, 589, 1188
HpyCH4V TGCA 7 cut(s) 116, 280, 396, 728, 869, 966, 1334
HpyF10VI GCNNNNNNNGC 9 cut(s) 113, 155, 230, 277, 338, 402, 593, 1178, 1323
HpyF3I CTNAG 8 cut(s) 194, 414, 520, 555, 622, 702, 766, 1599
HpySE526I ACGT 3 cut(s) 303, 589, 1188
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 9 cut(s) 575, 905, 1180, 1195, 1300, 1316, 1417, 1491, 1583
HspAI GCGC 3 cut(s) 222, 1078, 1533
KasI GGCGCC 1 cut(s) 221
KflI GGGWCCC 1 cut(s) 65
KpnI GGTACC 1 cut(s) 133
KroI GCCGGC 2 cut(s) 34, 463
KroNI GCCGGC 2 cut(s) 36, 465
Ksp22I TGATCA 1 cut(s) 913
KspI CCGCGG 1 cut(s) 136
Kzo9I GATC 7 cut(s) 159, 261, 524, 913, 1027, 1215, 1499
LguI GCTCTTC 1 cut(s) 900
LmnI GCTCC 7 cut(s) 276, 313, 321, 343, 1342, 1346, 1408
Lsp1109I GCAGC 5 cut(s) 10, 414, 853, 1012, 1346
LweI GCATC 4 cut(s) 66, 103, 267, 1230
MaeI CTAG 1 cut(s) 1151
MaeII ACGT 3 cut(s) 303, 589, 1188
MaeIII GTNAC 2 cut(s) 478, 1087
MalI GATC 7 cut(s) 161, 263, 526, 915, 1029, 1217, 1501
MbiI CCGCTC 1 cut(s) 346
MboI GATC 7 cut(s) 159, 261, 524, 913, 1027, 1215, 1499
MboII GAAGA 7 cut(s) 346, 455, 497, 500, 887, 1037, 1514
MfeI CAATTG 1 cut(s) 391
MflI RGATCY 2 cut(s) 261, 524
MluCI AATT 9 cut(s) 120, 256, 391, 541, 713, 834, 885, 936, 1256
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 5 cut(s) 512, 692, 1241, 1245, 1598
MmeI TCCRAC 2 cut(s) 1275, 1304
MroNI GCCGGC 2 cut(s) 34, 463
MseI TTAA 4 cut(s) 654, 671, 801, 1618
MslI CAYNNNNRTG 1 cut(s) 1085
MspA1I CMGCKG 4 cut(s) 23, 135, 198, 1120
MspI CCGG 7 cut(s) 35, 95, 266, 464, 500, 1406, 1568
MspR9I CCNGG 4 cut(s) 266, 599, 1568, 1605
MunI CAATTG 1 cut(s) 391
MvaI CCWGG 2 cut(s) 599, 1605
MvnI CGCG 5 cut(s) 101, 135, 158, 236, 1533
MwoI GCNNNNNNNGC 9 cut(s) 113, 155, 230, 277, 338, 402, 593, 1178, 1323
NaeI GCCGGC 2 cut(s) 36, 465
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 2 cut(s) 266, 1568
NcoI CCATGG 1 cut(s) 1487
NdeI CATATG 2 cut(s) 648, 1330
NdeII GATC 7 cut(s) 159, 261, 524, 913, 1027, 1215, 1499
NgoMIV GCCGGC 2 cut(s) 34, 463
NlaIII CATG 9 cut(s) 575, 905, 1180, 1195, 1300, 1316, 1417, 1491, 1583
NmeAIII GCCGAG 2 cut(s) 409, 1597
NmuCI GTSAC 2 cut(s) 478, 1087
NotI GCGGCCGC 1 cut(s) 147
NspI RCATGY 1 cut(s) 1583
OliI CACNNNNGTG 1 cut(s) 1085
PciI ACATGT 1 cut(s) 1579
PciSI GCTCTTC 1 cut(s) 900
PdiI GCCGGC 2 cut(s) 36, 465
PfeI GAWTC 3 cut(s) 632, 823, 1058
PflMI CCANNNNNTGG 1 cut(s) 845
PleI GAGTC 5 cut(s) 512, 692, 1241, 1244, 1597
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 5 cut(s) 512, 692, 1241, 1244, 1597
PpuMI RGGWCCY 1 cut(s) 65
PscI ACATGT 1 cut(s) 1579
Psp124BI GAGCTC 3 cut(s) 318, 708, 765
Psp5II RGGWCCY 1 cut(s) 65
Psp6I CCWGG 2 cut(s) 597, 1603
PspGI CCWGG 2 cut(s) 597, 1603
PspPI GGNCC 5 cut(s) 17, 65, 210, 881, 1115
PspPPI RGGWCCY 1 cut(s) 65
PsuI RGATCY 2 cut(s) 261, 524
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 4 cut(s) 131, 287, 924, 1471
RsaNI GTAC 4 cut(s) 130, 286, 923, 1470
RseI CAYNNNNRTG 1 cut(s) 1085
SacI GAGCTC 3 cut(s) 318, 708, 765
SacII CCGCGG 1 cut(s) 136
SapI GCTCTTC 1 cut(s) 900
SaqAI TTAA 4 cut(s) 654, 671, 801, 1618
Sau3AI GATC 7 cut(s) 159, 261, 524, 913, 1027, 1215, 1499
Sau96I GGNCC 5 cut(s) 17, 65, 210, 881, 1115
SchI GAGTC 5 cut(s) 512, 692, 1241, 1245, 1598
ScrFI CCNGG 4 cut(s) 266, 599, 1568, 1605
SfaNI GCATC 4 cut(s) 66, 103, 267, 1230
SfoI GGCGCC 1 cut(s) 223
Sfr303I CCGCGG 1 cut(s) 136
SgrBI CCGCGG 1 cut(s) 136
SinI GGWCC 1 cut(s) 65
SmiMI CAYNNNNRTG 1 cut(s) 1085
SmlI CTYRAG 1 cut(s) 528
SmoI CTYRAG 1 cut(s) 528
Sse9I AATT 9 cut(s) 120, 256, 391, 541, 713, 834, 885, 936, 1256
SspDI GGCGCC 1 cut(s) 221
SspI AATATT 3 cut(s) 690, 843, 995
SspMI CTAG 1 cut(s) 1151
SstI GAGCTC 3 cut(s) 318, 708, 765
StyD4I CCNGG 4 cut(s) 264, 597, 1566, 1603
StyI CCWWGG 1 cut(s) 1487
TaaI ACNGT 8 cut(s) 88, 494, 581, 927, 1169, 1303, 1495, 1615
TaiI ACGT 3 cut(s) 306, 592, 1191
TaqI TCGA 1 cut(s) 1450
TasI AATT 9 cut(s) 120, 256, 391, 541, 713, 834, 885, 936, 1256
TatI WGTACW 1 cut(s) 922
TauI GCSGC 8 cut(s) 34, 138, 149, 152, 201, 236, 470, 1120
TfiI GAWTC 3 cut(s) 632, 823, 1058
Tru1I TTAA 4 cut(s) 654, 671, 801, 1618
Tru9I TTAA 4 cut(s) 654, 671, 801, 1618
TscAI CASTG 5 cut(s) 487, 552, 559, 1174, 1618
TseFI GTSAC 2 cut(s) 478, 1087
TseI GCWGC 5 cut(s) 23, 402, 866, 1000, 1334
Tsp45I GTSAC 2 cut(s) 478, 1087
TspDTI ATGAA 2 cut(s) 269, 876
TspRI CASTG 5 cut(s) 487, 552, 559, 1174, 1618
Van91I CCANNNNNTGG 1 cut(s) 845
VpaK11BI GGWCC 1 cut(s) 65
XapI RAATTY 1 cut(s) 834
XceI RCATGY 1 cut(s) 1583
XmiI GTMKAC 1 cut(s) 1245
XspI CTAG 1 cut(s) 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.