Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0003789

Physical Location & Seq

Genomic Coordinates Forward (+)

63326725 .. 63333522

Gene Structure 6798 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG446500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGCCGGCGATGAGAGATGGTGCGTCGTCACCGGAGGCCGAGGCTTCGCCGCTCGCCATTTGGTTCTCATGCTCATCCGCCAAAACGTCTTCCGCGTCCGAATCGCCGACCTCGGCCCCACCGTCGATCTCGAACCTTCCGAGTCCAACGGAGTCCTCGCCGATGCTTTGAACTCCGGCCGCGCCCAGTACATTTCCATCGACCTCCGCCGTAAGGATCAAGTTCTTCAAGCATGTCAAGGTGCTGAGGTTGTGTTCCACATGGCGGCTCCCAATTCGTCGATCAACAATTACCAGCTCCACCATTCGGTCAATGTGGAAGGAACTAAGAATGTGATTGATGCTTGTGTTGAGCAGAAAGTGAAGAGGCTCATATACACTAGCTCTCCTAGTGTTGTGTTTGATGGGGTTCATGGCATTATTGATGGGGATGAGTCATTGCCATATCCACCTAAGCACAATGATACTTATTCGGCCACCAAAGCGCAAGGAGAAGAGTTGGTAATCAAGGCAAACGGTGTTAAAGGGCTTCTAACCTGCTGCATACGCCCTAGCAGCATTTTCGGACCCGGTGATAGGTTACTTGTTCCATCTTTAATTGTTGCCGCCAGGGCAGGGAAATCCAAGTTCATTATCGGAGATGGCAATAACATTTATGACTTCACGTATGTTGAAAACGTGGCCCATGCCCATATATGTGCAGAACGAGCTCTAGCGTCTGAAGGGACAGTTGCAGAGAAAGCTGCAGGACAGGCATATTTTATAACCAATATGGAACCTATCAAGTTTTGGGAGTTTATGTCACTTATTCTAGAAGGTCTTGGCTATGAAAGACCAAGAATAAAAATCCCTGCAGCTGTTATGATGCCAATTGCACATGTGGTGGAGTGGACATTTAAGCTTTTTGGTCCATATGGGATGAAGGTTCCACAGTTGACGCCTTCAAGAATTAGACTTCTCTCTTGCACCAGATCTTTTAAATCTTCAAAAGCACAGGATCGAATTGGTTACACGCCCATCATTTCGCTTCAGGAGGGTCTTAAGAGGACAATCGAGTCATACCCACACTTGAGGGCTGAACATCGACCCAAAAGAGAAGGGCCATCTAAAGCTTCTATGTATCTTGGAAGTGGGAGGGTTGCTGATGCATTACTGTGGAAGGACAAAAAACAGACATTCAAGGCACTATTATTTTTTATTGCAATCTACTACAACTTCATTGCATCTCAAGCTACAGTTATTACCGTCATTTCAAAGAGTCTCATGTTCGCATCAATTTTTGTGTTCATCTCTGGCAATTTACCAGAAAAGATGATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0008150 GO:0008152 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0047012 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01130 map00100 map01100 map01130

Pfam Domains

Protein Families

3Beta_HSD Reticulon

Protein Analysis

439

Amino Acids

48.53

Weight (kDa)

9.2

Isoelectric Point (pI)

38.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
3Beta_HSD	PF01073	9 - 283	1.4e-78	3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase	PF01370	9 - 237	1.7e-34	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	9 - 129	1e-07	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	10 - 237	8.3e-15	GDP-mannose 4,6 dehydratase
NAD_binding_10	PF13460	57 - 133	1.6e-08	NAD(P)H-binding
RmlD_sub_bind	PF04321	66 - 282	3.2e-08	RmlD substrate binding domain
NAD_binding_4	PF07993	76 - 229	8.7e-14	Male sterility protein
Reticulon	PF02453	380 - 435	1.2e-08	Reticulon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003789)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47290 AT1G47290 AT2G26260 AT2G26260
fragaria_vesca	FvH4_2g36970 FvH4_2g36970
malus_domestica	MD08G1046500.v1.1
prunus_persica	Prupe.1G401700_v2.0.a1 Prupe.1G401700_v2.0.a1
pyrus_communis	pycom08g03860 pycom15g04580 pycom15g04640
rosa_chinensis	RchiOBHm_Chr4g0406951 RchiOBHm_Chr6g0304791
rosa_laevigata	RLG00000008747 RLG00000010949
rosa_multiflora	Rmu_sc0000600.1_g000025 Rmu_sc0003120.1_g000003
rosa_roxburghii	Rroxscaffold_7G00163470
rosa_rugosa	Rorug03G0259800 Rorug04G0075400 Rorug06G0334600 Rorug06G0334600 Rorug06G0334700 Rorug06G0334800
rosa_samantha	Rh4AG141600 Rh4BG138400 Rh4DG135500 Rh6AG446500 Rh6BG455100 Rh6CG460000 Rh6DG446800
rosa_wichuraiana	Rw4G011530 Rw6G038850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	764
AasI	GACNNNNNNGTC	1 cut(s)	1054
Acc36I	ACCTGC	1 cut(s)	545
AccBSI	CCGCTC	1 cut(s)	53
AccII	CGCG	2 cut(s)	96, 183
AciI	CCGC	7 cut(s)	51, 79, 94, 181, 208, 266, 606
AclWI	GGATC	2 cut(s)	225, 1005
AcoI	YGGCCR	3 cut(s)	3, 178, 474
AcuI	CTGAAG	2 cut(s)	741, 1013
AcyI	GRCGYC	1 cut(s)	938
AfaI	GTAC	1 cut(s)	191
AfiI	CCNNNNNNNGG	5 cut(s)	214, 265, 307, 576, 615
AflII	CTTAAG	1 cut(s)	1040
AflIII	ACRYGT	1 cut(s)	877
AgsI	TTSAA	7 cut(s)	172, 230, 674, 945, 987, 1180, 1254
AjnI	CCWGG	1 cut(s)	608
AluBI	AGCT	8 cut(s)	298, 384, 710, 743, 857, 901, 1112, 1232
AluI	AGCT	8 cut(s)	298, 384, 710, 743, 857, 901, 1112, 1232
Alw21I	GWGCWC	1 cut(s)	712
Alw26I	GTCTC	1 cut(s)	1265
AlwI	GGATC	2 cut(s)	225, 1005
AoxI	GGCC	7 cut(s)	3, 37, 115, 178, 474, 681, 1100
ApeKI	GCWGC	4 cut(s)	540, 555, 743, 854
AspLEI	GCGC	2 cut(s)	185, 487
AspS9I	GGNCC	5 cut(s)	116, 566, 682, 908, 1100
AsuC2I	CCSGG	1 cut(s)	570
AsuHPI	GGTGA	2 cut(s)	22, 584
AvaII	GGWCC	2 cut(s)	566, 908
BanII	GRGCYC	1 cut(s)	712
BbsI	GAAGAC	1 cut(s)	82
Bbv12I	GWGCWC	1 cut(s)	712
BbvCI	CCTCAGC	1 cut(s)	246
BbvI	GCAGC	4 cut(s)	527, 567, 730, 866
BccI	CCATC	8 cut(s)	12, 206, 398, 419, 598, 635, 1025, 1111
BceAI	ACGGC	1 cut(s)	195
BcgI	CGANNNNNNTGC	2 cut(s)	544, 578
BciT130I	CCWGG	1 cut(s)	610
BcnI	CCSGG	1 cut(s)	570
BcoDI	GTCTC	1 cut(s)	1265
BfaI	CTAG	5 cut(s)	381, 390, 552, 713, 812
BfmI	CTRYAG	3 cut(s)	744, 852, 1233
BfrI	CTTAAG	1 cut(s)	1040
BfuAI	ACCTGC	1 cut(s)	545
BglI	GCCNNNNNGGC	1 cut(s)	611
BglII	AGATCT	1 cut(s)	971
BisI	GCNGC	8 cut(s)	51, 181, 267, 541, 556, 606, 744, 855
BlsI	GCNGC	8 cut(s)	52, 182, 268, 542, 557, 607, 745, 856
Bme1390I	CCNGG	2 cut(s)	570, 610
Bme18I	GGWCC	2 cut(s)	566, 908
BmgT120I	GGNCC	5 cut(s)	116, 566, 682, 908, 1100
BmiI	GGNNCC	5 cut(s)	118, 270, 568, 777, 927
BmrFI	CCNGG	2 cut(s)	570, 610
BmrI	ACTGGG	1 cut(s)	181
BmsI	GCATC	6 cut(s)	154, 331, 855, 1135, 1232, 1280
BmuI	ACTGGG	1 cut(s)	181
BpiI	GAAGAC	1 cut(s)	82
Bpu10I	CCTNAGC	2 cut(s)	246, 453
BpuEI	CTTGAG	2 cut(s)	1090, 1212
BpuMI	CCSGG	1 cut(s)	570
BsaAI	YACGTR	1 cut(s)	666
BsaHI	GRCGYC	1 cut(s)	938
BsaJI	CCNNGG	3 cut(s)	40, 112, 609
BsaWI	WCCGGW	1 cut(s)	32
BsaXI	ACNNNNNCTCC	4 cut(s)	370, 400, 785, 815
Bsc4I	CCNNNNNNNGG	5 cut(s)	214, 265, 307, 576, 615
Bse118I	RCCGGY	1 cut(s)	5
Bse1I	ACTGG	1 cut(s)	187
Bse3DI	GCAATG	2 cut(s)	437, 1218
BseBI	CCWGG	1 cut(s)	610
BseDI	CCNNGG	3 cut(s)	40, 112, 609
BseGI	GGATG	3 cut(s)	75, 436, 924
BseLI	CCNNNNNNNGG	5 cut(s)	214, 265, 307, 576, 615
BseMI	GCAATG	2 cut(s)	437, 1218
BseMII	CTCAG	1 cut(s)	237
BseNI	ACTGG	1 cut(s)	187
BseX3I	CGGCCG	1 cut(s)	178
BseXI	GCAGC	4 cut(s)	527, 567, 730, 866
BsgI	GTGCAG	1 cut(s)	720
Bsh1236I	CGCG	2 cut(s)	96, 183
Bsh1285I	CGRYCG	1 cut(s)	181
BshFI	GGCC	7 cut(s)	5, 39, 117, 180, 476, 683, 1102
BsiEI	CGRYCG	1 cut(s)	181
BsiHKAI	GWGCWC	1 cut(s)	712
BsiSI	CCGG	4 cut(s)	6, 33, 177, 570
BslFI	GGGAC	1 cut(s)	739
BslI	CCNNNNNNNGG	5 cut(s)	214, 265, 307, 576, 615
BsmAI	GTCTC	1 cut(s)	1265
BsmFI	GGGAC	1 cut(s)	739
BsnI	GGCC	7 cut(s)	5, 39, 117, 180, 476, 683, 1102
Bsp1286I	GDGCHC	1 cut(s)	712
Bsp143I	GATC	5 cut(s)	127, 217, 282, 971, 997
BspACI	CCGC	7 cut(s)	51, 79, 94, 181, 208, 266, 606
BspANI	GGCC	7 cut(s)	5, 39, 117, 180, 476, 683, 1102
BspCNI	CTCAG	1 cut(s)	238
BspFNI	CGCG	2 cut(s)	96, 183
BspLI	GGNNCC	5 cut(s)	118, 270, 568, 777, 927
BspMAI	CTGCAG	2 cut(s)	748, 856
BspMI	ACCTGC	1 cut(s)	545
BspPI	GGATC	2 cut(s)	225, 1005
BspTI	CTTAAG	1 cut(s)	1040
BsrBI	CCGCTC	1 cut(s)	53
BsrDI	GCAATG	2 cut(s)	437, 1218
BsrFI	RCCGGY	1 cut(s)	5
BsrI	ACTGG	1 cut(s)	187
BssAI	RCCGGY	1 cut(s)	5
BssECI	CCNNGG	3 cut(s)	40, 112, 609
BssMI	GATC	5 cut(s)	127, 217, 282, 971, 997
BssNI	GRCGYC	1 cut(s)	938
Bst2UI	CCWGG	1 cut(s)	610
Bst4CI	ACNGT	7 cut(s)	124, 518, 730, 933, 1155, 1237, 1246
Bst6I	CTCTTC	2 cut(s)	359, 489
BstACI	GRCGYC	1 cut(s)	938
BstAFI	CTTAAG	1 cut(s)	1040
BstBAI	YACGTR	1 cut(s)	666
BstC8I	GCNNGC	2 cut(s)	7, 55
BstDEI	CTNAG	3 cut(s)	246, 327, 453
BstF5I	GGATG	3 cut(s)	75, 436, 924
BstFNI	CGCG	2 cut(s)	96, 183
BstHHI	GCGC	2 cut(s)	185, 487
BstKTI	GATC	5 cut(s)	130, 220, 285, 974, 1000
BstMAI	GTCTC	1 cut(s)	1265
BstMBI	GATC	5 cut(s)	127, 217, 282, 971, 997
BstMCI	CGRYCG	1 cut(s)	181
BstMWI	GCNNNNNNNGC	8 cut(s)	482, 546, 555, 611, 707, 740, 752, 1229
BstNI	CCWGG	1 cut(s)	610
BstNSI	RCATGY	2 cut(s)	237, 881
BstSCI	CCNGG	2 cut(s)	568, 608
BstSFI	CTRYAG	3 cut(s)	744, 852, 1233
BstUI	CGCG	2 cut(s)	96, 183
BstV1I	GCAGC	4 cut(s)	527, 567, 730, 866
BstV2I	GAAGAC	1 cut(s)	82
BstX2I	RGATCY	1 cut(s)	971
BstYI	RGATCY	1 cut(s)	971
BstZI	CGGCCG	1 cut(s)	178
BsuRI	GGCC	7 cut(s)	5, 39, 117, 180, 476, 683, 1102
BtgZI	GCGATG	1 cut(s)	24
BtsCI	GGATG	3 cut(s)	75, 436, 924
BveI	ACCTGC	1 cut(s)	545
Cac8I	GCNNGC	2 cut(s)	7, 55
CfoI	GCGC	2 cut(s)	185, 487
Cfr10I	RCCGGY	1 cut(s)	5
Cfr13I	GGNCC	5 cut(s)	116, 566, 682, 908, 1100
CseI	GACGC	4 cut(s)	13, 85, 705, 946
Csp6I	GTAC	1 cut(s)	190
CviAII	CATG	7 cut(s)	70, 234, 262, 413, 686, 878, 1264
CviQI	GTAC	1 cut(s)	190
DdeI	CTNAG	3 cut(s)	246, 327, 453
DpnI	GATC	5 cut(s)	129, 219, 284, 973, 999
DpnII	GATC	5 cut(s)	127, 217, 282, 971, 997
DraI	TTTAAA	1 cut(s)	979
DrdI	GACNNNNNNGTC	1 cut(s)	1054
DseDI	GACNNNNNNGTC	1 cut(s)	1054
EaeI	YGGCCR	3 cut(s)	3, 178, 474
EagI	CGGCCG	1 cut(s)	178
Eam1104I	CTCTTC	2 cut(s)	359, 489
EarI	CTCTTC	2 cut(s)	359, 489
EciI	GGCGGA	2 cut(s)	68, 197
Ecl136II	GAGCTC	1 cut(s)	710
EclXI	CGGCCG	1 cut(s)	178
Eco24I	GRGCYC	1 cut(s)	712
Eco47I	GGWCC	2 cut(s)	566, 908
Eco52I	CGGCCG	1 cut(s)	178
Eco53kI	GAGCTC	1 cut(s)	710
Eco57I	CTGAAG	2 cut(s)	741, 1013
EcoICRI	GAGCTC	1 cut(s)	710
EcoRII	CCWGG	1 cut(s)	608
EcoT22I	ATGCAT	1 cut(s)	1150
EcoT38I	GRGCYC	1 cut(s)	712
FaeI	CATG	7 cut(s)	73, 237, 265, 416, 689, 881, 1267
FaqI	GGGAC	1 cut(s)	739
FatI	CATG	7 cut(s)	69, 233, 261, 412, 685, 877, 1263
FauNDI	CATATG	1 cut(s)	913
Fnu4HI	GCNGC	8 cut(s)	51, 181, 267, 541, 556, 606, 744, 855
FokI	GGATG	3 cut(s)	62, 443, 931
FriOI	GRGCYC	1 cut(s)	712
Fsp4HI	GCNGC	8 cut(s)	51, 181, 267, 541, 556, 606, 744, 855
FspBI	CTAG	5 cut(s)	381, 390, 552, 713, 812
GlaI	GCGC	2 cut(s)	184, 486
GluI	GCNGC	8 cut(s)	51, 181, 267, 541, 556, 606, 744, 855
HaeIII	GGCC	7 cut(s)	5, 39, 117, 180, 476, 683, 1102
HapII	CCGG	4 cut(s)	6, 33, 177, 570
HgaI	GACGC	4 cut(s)	13, 85, 705, 946
HhaI	GCGC	2 cut(s)	185, 487
Hin1I	GRCGYC	1 cut(s)	938
Hin1II	CATG	7 cut(s)	73, 237, 265, 416, 689, 881, 1267
Hin6I	GCGC	2 cut(s)	183, 485
HinP1I	GCGC	2 cut(s)	183, 485
HincII	GTYRAC	1 cut(s)	936
HindII	GTYRAC	1 cut(s)	936
HindIII	AAGCTT	2 cut(s)	899, 1110
HinfI	GANTC	6 cut(s)	102, 143, 153, 434, 1055, 1258
HpaII	CCGG	4 cut(s)	6, 33, 177, 570
HphI	GGTGA	2 cut(s)	22, 584
Hpy166II	GTNNAC	2 cut(s)	891, 936
Hpy188I	TCNGA	5 cut(s)	101, 142, 566, 638, 721
Hpy188III	TCNNGA	4 cut(s)	131, 812, 945, 1031
Hpy8I	GTNNAC	2 cut(s)	891, 936
Hpy99I	CGWCG	3 cut(s)	29, 128, 283
HpyAV	CCTTC	8 cut(s)	147, 314, 716, 809, 916, 951, 1091, 1153
HpyCH4III	ACNGT	7 cut(s)	124, 518, 730, 933, 1155, 1237, 1246
HpyCH4IV	ACGT	3 cut(s)	87, 665, 678
HpyF10VI	GCNNNNNNNGC	8 cut(s)	482, 546, 555, 611, 707, 740, 752, 1229
HpyF3I	CTNAG	3 cut(s)	246, 327, 453
HpySE526I	ACGT	3 cut(s)	87, 665, 678
Hsp92I	GRCGYC	1 cut(s)	938
Hsp92II	CATG	7 cut(s)	73, 237, 265, 416, 689, 881, 1267
HspAI	GCGC	2 cut(s)	183, 485
KroI	GCCGGC	1 cut(s)	5
KroNI	GCCGGC	1 cut(s)	7
Kzo9I	GATC	5 cut(s)	127, 217, 282, 971, 997
LmnI	GCTCC	2 cut(s)	274, 303
Lsp1109I	GCAGC	4 cut(s)	527, 567, 730, 866
LweI	GCATC	6 cut(s)	154, 331, 855, 1135, 1232, 1280
MaeI	CTAG	5 cut(s)	381, 390, 552, 713, 812
MaeII	ACGT	3 cut(s)	87, 665, 678
MaeIII	GTNAC	4 cut(s)	28, 579, 801, 1007
MalI	GATC	5 cut(s)	129, 219, 284, 973, 999
MbiI	CCGCTC	1 cut(s)	53
MboI	GATC	5 cut(s)	127, 217, 282, 971, 997
MboII	GAAGA	5 cut(s)	82, 218, 376, 506, 975
MfeI	CAATTG	1 cut(s)	870
MflI	RGATCY	1 cut(s)	971
MhlI	GDGCHC	1 cut(s)	712
MluCI	AATT	8 cut(s)	274, 289, 597, 870, 948, 1002, 1275, 1297
MlyI	GAGTC	5 cut(s)	152, 162, 443, 1064, 1267
MmeI	TCCRAC	1 cut(s)	171
Mph1103I	ATGCAT	1 cut(s)	1150
MroNI	GCCGGC	1 cut(s)	5
MseI	TTAA	5 cut(s)	522, 596, 897, 978, 1041
MslI	CAYNNNNRTG	2 cut(s)	696, 1153
MspA1I	CMGCKG	1 cut(s)	857
MspCI	CTTAAG	1 cut(s)	1040
MspI	CCGG	4 cut(s)	6, 33, 177, 570
MspR9I	CCNGG	2 cut(s)	570, 610
MunI	CAATTG	1 cut(s)	870
MvaI	CCWGG	1 cut(s)	610
MvnI	CGCG	2 cut(s)	96, 183
MwoI	GCNNNNNNNGC	8 cut(s)	482, 546, 555, 611, 707, 740, 752, 1229
NaeI	GCCGGC	1 cut(s)	7
NciI	CCSGG	1 cut(s)	570
NdeI	CATATG	1 cut(s)	913
NdeII	GATC	5 cut(s)	127, 217, 282, 971, 997
NgoMIV	GCCGGC	1 cut(s)	5
NlaIII	CATG	7 cut(s)	73, 237, 265, 416, 689, 881, 1267
NlaIV	GGNNCC	5 cut(s)	118, 270, 568, 777, 927
NmeAIII	GCCGAG	2 cut(s)	65, 93
NmuCI	GTSAC	2 cut(s)	28, 801
NsiI	ATGCAT	1 cut(s)	1150
NspI	RCATGY	2 cut(s)	237, 881
PciI	ACATGT	1 cut(s)	877
PcsI	WCGNNNNNNNCGW	3 cut(s)	93, 120, 138
PdiI	GCCGGC	1 cut(s)	7
PfeI	GAWTC	1 cut(s)	102
PkrI	GCNGC	8 cut(s)	52, 182, 268, 542, 557, 607, 745, 856
PleI	GAGTC	5 cut(s)	151, 161, 442, 1063, 1266
PpsI	GAGTC	5 cut(s)	151, 161, 442, 1063, 1266
Ppu21I	YACGTR	1 cut(s)	666
PscI	ACATGT	1 cut(s)	877
PsiI	TTATAA	1 cut(s)	764
Psp124BI	GAGCTC	1 cut(s)	712
Psp6I	CCWGG	1 cut(s)	608
PspGI	CCWGG	1 cut(s)	608
PspN4I	GGNNCC	5 cut(s)	118, 270, 568, 777, 927
PspPI	GGNCC	5 cut(s)	116, 566, 682, 908, 1100
PstI	CTGCAG	2 cut(s)	748, 856
PsuI	RGATCY	1 cut(s)	971
PvuII	CAGCTG	1 cut(s)	857
RsaI	GTAC	1 cut(s)	191
RsaNI	GTAC	1 cut(s)	190
RseI	CAYNNNNRTG	2 cut(s)	696, 1153
SacI	GAGCTC	1 cut(s)	712
SaqAI	TTAA	5 cut(s)	522, 596, 897, 978, 1041
SatI	GCNGC	8 cut(s)	51, 181, 267, 541, 556, 606, 744, 855
Sau3AI	GATC	5 cut(s)	127, 217, 282, 971, 997
Sau96I	GGNCC	5 cut(s)	116, 566, 682, 908, 1100
SchI	GAGTC	5 cut(s)	152, 162, 443, 1064, 1267
ScrFI	CCNGG	2 cut(s)	570, 610
SduI	GDGCHC	1 cut(s)	712
SfaNI	GCATC	6 cut(s)	154, 331, 855, 1135, 1232, 1280
SfcI	CTRYAG	3 cut(s)	744, 852, 1233
SinI	GGWCC	2 cut(s)	566, 908
SmiMI	CAYNNNNRTG	2 cut(s)	696, 1153
SmlI	CTYRAG	3 cut(s)	1040, 1069, 1227
SmoI	CTYRAG	3 cut(s)	1040, 1069, 1227
Sse9I	AATT	8 cut(s)	274, 289, 597, 870, 948, 1002, 1275, 1297
SsiI	CCGC	7 cut(s)	51, 79, 94, 181, 208, 266, 606
SspMI	CTAG	5 cut(s)	381, 390, 552, 713, 812
SstI	GAGCTC	1 cut(s)	712
StyD4I	CCNGG	2 cut(s)	568, 608
TaaI	ACNGT	7 cut(s)	124, 518, 730, 933, 1155, 1237, 1246
TaiI	ACGT	3 cut(s)	90, 668, 681
TaqI	TCGA	7 cut(s)	126, 132, 201, 281, 1000, 1053, 1084
TaqII	GACCGA	1 cut(s)	298
TasI	AATT	8 cut(s)	274, 289, 597, 870, 948, 1002, 1275, 1297
TatI	WGTACW	1 cut(s)	189
TauI	GCSGC	4 cut(s)	53, 183, 269, 608
TfiI	GAWTC	1 cut(s)	102
Tru1I	TTAA	5 cut(s)	522, 596, 897, 978, 1041
Tru9I	TTAA	5 cut(s)	522, 596, 897, 978, 1041
TseFI	GTSAC	2 cut(s)	28, 801
TseI	GCWGC	4 cut(s)	540, 555, 743, 854
Tsp45I	GTSAC	2 cut(s)	28, 801
TspDTI	ATGAA	6 cut(s)	401, 619, 843, 935, 1207, 1276
TspGWI	ACGGA	1 cut(s)	165
Vha464I	CTTAAG	1 cut(s)	1040
VpaK11BI	GGWCC	2 cut(s)	566, 908
XbaI	TCTAGA	1 cut(s)	811
XceI	RCATGY	2 cut(s)	237, 881
XspI	CTAG	5 cut(s)	381, 390, 552, 713, 812
Zsp2I	ATGCAT	1 cut(s)	1150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.