Rh4BG138400

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003789
Physical Location & Seq
Forward (+)
24666751 .. 24675977
9227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG138400.1

Sequence Viewer

Length: 1842 bp
ATGACCTGCAATGAGAGATGGTGCGTTGTCACCGGAGGCCGAGGCTTCGCCGCTCGCCATTTGGTTCTCATGCTCATCCGCCAGAACGTCTTCCATGTCCGAATTGCTGACCTCGGCCCCACCATCGATCTCGAACCTTCCGAGTCCAACGGAGTCCTCGCCGATGCTTTGAACTTCGGCCGCGCTGAGTACATTTCCATCGACCTCCGCTGCAAGGATCAAGTTCTTCAAGCATGTCAAGGTGCTGAGGTTGTGTTCCATATGGCGGCACCTAATTCGTCAATCAACAATTACCAGCTCCACCATTCGGTCAATGTGGAAGGAACTAAAAATGTGATTGATGCTTGTGTTAAGCAGAAAGTTAAGAGGCTCATATACACTAGCTCTCCTAGCGTTGTGTTTGATGGGGTTCATGGTATTATTGATGGGGATGAGTCGTTGTCATATCCACCCAAGCACAATGATACTTATTCGGCCACTAAAGCGCAAGGAGAAGAGTTGGTAATCAAGGCAAACGGTGTTAAAGGGCTTCTAACCTGCTGCTTACGCCCTAGCAGCATTTTCGGACCCGGTGATAGGTTACTGGTTCCGTCTTTAGTTGTTGCCGCCAGGGCAGGGAAATCCAAGTTCATTATAGGAGATGGCAATAACATTTATGATTTCACGTATGTTGAAAACGTGGCCCATGCCCATATATGTGCAGAGCAAGCTCTAGCTTTTGAAGGCACAGTTGCAGAGAAAGCTGCAGGACAGGCATATTTTATAACCAATATGGAACCTATTAAGTTTTGGGAGTTTATTTCACTTATTCTAGAAGGTCTTGGTTATGAAAGACCAAGGATAAAAATCCCTACAGCTGTTATGATGCCAATTGCACATGTGGTGGCGTGGACATATAAGCTTTTAGGCCCGTATGGGATGAAGATTCCACAGTTGACGCCTTCAAGAATTAGACTTCTCTCTTGCACCAGATCTTTTAAATCTTCAAAAGCACAGGATCGAATTGGTTACACACCCATTATTTCGCTTCAGGAGGGTCTTAAGAGGACAATTGAGTCATACCCACACTTGAGGGCTGAACATCGACCCAAAAGAGAAGGGCCATCTAAAGCTTCTGTATATCTTGGAAGCGGAAGGGTTGCTGACGCATTACTGTGGAGGGATAAAAAACAGACATTCAAGGCACTATTAGTTTTGATTGCGATCTACTACAACTTCATTGAATCTAAAGCTACGATTATTACTGTCATTTCGAAGAGTCTCATGTTCGCATCAATTTTCGTGTTCATCTCGGGCAATTTACCAGAAATGATATTAGGATATAAACTCGAGAAGCTTCAATCATCACATTTTCACTTCTCAGAAGAGATGTCACAACAAATTGCTGCATCTGTGGTCTCATCATGGAATATATCTGTCAAAACTTTAAAATCTCTCGGGACAGGAAATGACTGCATATTGTTTCTTAAGGCGGCTGTATCTCTACTGGTTCTTAGCTTTCTTGGAGCTATTTCACTTCAGAACTCATTTATTATAGGAGTTTCCACTGCCTTTTTAGCCTTCTTTGTGTATGAAAAGCAGGAAGAAAAAATCGATGGCATGGTTCTTGAAGCTTTCTCTCGTGGACGCAAATTGAATTCGCAATACCCCAAACCTAATAGCGAAAATCCCAGTCCTTCCGGCGAGTTCTCCTTCACAAGTTCTTCTTCCCGTCTAATCACCCAATTCTCAAGAATCTCAGAGGACCCAAAAGAGAGAGTAGCACAGACCGCAAATCAACAAATTGCTTCAGCTACACTTTTTCAACCCTTGGAGATATCAACATTGACAGTTCAGTGCTAG

Protein Analysis

613

Amino Acids

67.67

Weight (kDa)

8.91

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 9 - 283 2.6e-77 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 9 - 237 8.3e-34 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 129 9.3e-07 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 10 - 243 5.2e-13 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 60 - 133 2.5e-07 NAD(P)H-binding
NAD_binding_4 PF07993 77 - 228 1.4e-13 Male sterility protein
RmlD_sub_bind PF04321 82 - 282 4e-07 RmlD substrate binding domain
Reticulon PF02453 380 - 535 7.1e-31 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 764
AasI GACNNNNNNGTC 1 cut(s) 1054
Acc36I ACCTGC 2 cut(s) 14, 545
AccB1I GGYRCC 1 cut(s) 268
AccBSI CCGCTC 1 cut(s) 53
AccII CGCG 1 cut(s) 183
AciI CCGC 9 cut(s) 51, 79, 181, 208, 266, 606, 1131, 1472, 1770
AclWI GGATC 2 cut(s) 225, 1005
AcoI YGGCCR 2 cut(s) 178, 474
AcsI RAATTY 1 cut(s) 1636
AcuI CTGAAG 3 cut(s) 1013, 1502, 1773
AcyI GRCGYC 1 cut(s) 938
AfaI GTAC 1 cut(s) 191
AfiI CCNNNNNNNGG 5 cut(s) 214, 265, 307, 576, 615
AflII CTTAAG 2 cut(s) 1040, 1466
AflIII ACRYGT 1 cut(s) 877
AjnI CCWGG 1 cut(s) 608
Alw26I GTCTC 2 cut(s) 1265, 1402
AlwI GGATC 2 cut(s) 225, 1005
Ama87I CYCGRG 3 cut(s) 1291, 1328, 1436
AoxI GGCC 7 cut(s) 37, 115, 178, 474, 681, 907, 1100
ApeKI GCWGC 5 cut(s) 210, 540, 555, 743, 1385
ApoI RAATTY 1 cut(s) 1636
Asp700I GAANNNNTTC 1 cut(s) 89
AspLEI GCGC 2 cut(s) 185, 487
AspS9I GGNCC 6 cut(s) 116, 566, 682, 908, 1100, 1744
AsuC2I CCSGG 1 cut(s) 570
AsuHPI GGTGA 3 cut(s) 22, 584, 1711
AsuII TTCGAA 1 cut(s) 1253
AvaI CYCGRG 3 cut(s) 1291, 1328, 1436
AvaII GGWCC 2 cut(s) 566, 1744
BanI GGYRCC 1 cut(s) 268
BauI CACGAG 1 cut(s) 1620
BbsI GAAGAC 1 cut(s) 82
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 5 cut(s) 197, 527, 567, 730, 1372
BccI CCATC 8 cut(s) 12, 131, 206, 398, 419, 635, 1111, 1589
BcgI CGANNNNNNTGC 4 cut(s) 258, 292, 544, 578
BciT130I CCWGG 1 cut(s) 610
BcnI CCSGG 1 cut(s) 570
BcoDI GTCTC 2 cut(s) 1265, 1402
BfaI CTAG 6 cut(s) 381, 390, 552, 713, 812, 1840
BfmI CTRYAG 2 cut(s) 744, 852
BfrI CTTAAG 2 cut(s) 1040, 1466
BfuAI ACCTGC 2 cut(s) 14, 545
BglI GCCNNNNNGGC 1 cut(s) 611
BglII AGATCT 1 cut(s) 971
Bme1390I CCNGG 2 cut(s) 570, 610
Bme18I GGWCC 2 cut(s) 566, 1744
BmeT110I CYCGRG 3 cut(s) 1291, 1328, 1436
BmgT120I GGNCC 6 cut(s) 116, 566, 682, 908, 1100, 1744
BmiI GGNNCC 6 cut(s) 118, 270, 568, 588, 777, 1746
BmrFI CCNGG 2 cut(s) 570, 610
BmrI ACTGGG 1 cut(s) 1665
BmsI GCATC 5 cut(s) 154, 331, 855, 1280, 1397
BmuI ACTGGG 1 cut(s) 1665
BpiI GAAGAC 1 cut(s) 82
Bpu10I CCTNAGC 1 cut(s) 246
Bpu14I TTCGAA 1 cut(s) 1253
BpuEI CTTGAG 2 cut(s) 1090, 1714
BpuMI CCSGG 1 cut(s) 570
Bsa29I ATCGAT 2 cut(s) 126, 1593
BsaAI YACGTR 1 cut(s) 666
BsaBI GATNNNNATC 2 cut(s) 845, 1202
BsaHI GRCGYC 1 cut(s) 938
BsaI GGTCTC 1 cut(s) 1402
BsaJI CCNNGG 5 cut(s) 40, 112, 609, 836, 1809
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 5 cut(s) 214, 265, 307, 576, 615
Bse1I ACTGG 3 cut(s) 588, 1491, 1671
Bse3DI GCAATG 1 cut(s) 16
Bse8I GATNNNNATC 2 cut(s) 845, 1202
BseBI CCWGG 1 cut(s) 610
BseCI ATCGAT 2 cut(s) 126, 1593
BseDI CCNNGG 5 cut(s) 40, 112, 609, 836, 1809
BseGI GGATG 3 cut(s) 75, 436, 924
BseJI GATNNNNATC 2 cut(s) 845, 1202
BseLI CCNNNNNNNGG 5 cut(s) 214, 265, 307, 576, 615
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 4 cut(s) 177, 237, 1374, 1752
BseNI ACTGG 3 cut(s) 588, 1491, 1671
BseX3I CGGCCG 1 cut(s) 178
BseXI GCAGC 5 cut(s) 197, 527, 567, 730, 1372
BsgI GTGCAG 1 cut(s) 720
Bsh1236I CGCG 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 7 cut(s) 39, 117, 180, 476, 683, 909, 1102
BshNI GGYRCC 1 cut(s) 268
BshVI ATCGAT 2 cut(s) 126, 1593
BsiEI CGRYCG 1 cut(s) 181
BsiHKCI CYCGRG 3 cut(s) 1291, 1328, 1436
BsiSI CCGG 3 cut(s) 33, 570, 1680
BslFI GGGAC 1 cut(s) 1453
BslI CCNNNNNNNGG 5 cut(s) 214, 265, 307, 576, 615
BsmAI GTCTC 2 cut(s) 1265, 1402
BsmFI GGGAC 1 cut(s) 1453
BsnI GGCC 7 cut(s) 39, 117, 180, 476, 683, 909, 1102
Bso31I GGTCTC 1 cut(s) 1402
BsoBI CYCGRG 3 cut(s) 1291, 1328, 1436
Bsp119I TTCGAA 1 cut(s) 1253
Bsp143I GATC 5 cut(s) 127, 217, 971, 997, 1203
BspACI CCGC 9 cut(s) 51, 79, 181, 208, 266, 606, 1131, 1472, 1770
BspANI GGCC 7 cut(s) 39, 117, 180, 476, 683, 909, 1102
BspCNI CTCAG 4 cut(s) 178, 238, 1373, 1751
BspDI ATCGAT 2 cut(s) 126, 1593
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 6 cut(s) 118, 270, 568, 588, 777, 1746
BspMAI CTGCAG 1 cut(s) 748
BspMI ACCTGC 2 cut(s) 14, 545
BspPI GGATC 2 cut(s) 225, 1005
BspT104I TTCGAA 1 cut(s) 1253
BspT107I GGYRCC 1 cut(s) 268
BspTI CTTAAG 2 cut(s) 1040, 1466
BspTNI GGTCTC 1 cut(s) 1402
BsrBI CCGCTC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 16
BsrI ACTGG 3 cut(s) 588, 1491, 1671
BssECI CCNNGG 5 cut(s) 40, 112, 609, 836, 1809
BssMI GATC 5 cut(s) 127, 217, 971, 997, 1203
BssNI GRCGYC 1 cut(s) 938
BssSI CACGAG 1 cut(s) 1620
BssT1I CCWWGG 2 cut(s) 836, 1809
Bst2BI CACGAG 1 cut(s) 1620
Bst2UI CCWGG 1 cut(s) 610
Bst4CI ACNGT 6 cut(s) 518, 730, 933, 1155, 1246, 1831
Bst6I CTCTTC 3 cut(s) 489, 1250, 1359
BstACI GRCGYC 1 cut(s) 938
BstAFI CTTAAG 2 cut(s) 1040, 1466
BstBAI YACGTR 1 cut(s) 666
BstBI TTCGAA 1 cut(s) 1253
BstC8I GCNNGC 2 cut(s) 55, 708
BstDEI CTNAG 5 cut(s) 186, 246, 1360, 1493, 1738
BstF5I GGATG 3 cut(s) 75, 436, 924
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 2 cut(s) 185, 487
BstKTI GATC 5 cut(s) 130, 220, 974, 1000, 1206
BstMAI GTCTC 2 cut(s) 1265, 1402
BstMBI GATC 5 cut(s) 127, 217, 971, 997, 1203
BstMCI CGRYCG 1 cut(s) 181
BstNI CCWGG 1 cut(s) 610
BstNSI RCATGY 2 cut(s) 237, 881
BstSCI CCNGG 2 cut(s) 568, 608
BstSFI CTRYAG 2 cut(s) 744, 852
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 5 cut(s) 197, 527, 567, 730, 1372
BstV2I GAAGAC 1 cut(s) 82
BstX2I RGATCY 1 cut(s) 971
BstYI RGATCY 1 cut(s) 971
BstZI CGGCCG 1 cut(s) 178
Bsu15I ATCGAT 2 cut(s) 126, 1593
BsuRI GGCC 7 cut(s) 39, 117, 180, 476, 683, 909, 1102
BsuTUI ATCGAT 2 cut(s) 126, 1593
BtsCI GGATG 3 cut(s) 75, 436, 924
BtsI GCAGTG 1 cut(s) 1545
BtsIMutI CAGTG 2 cut(s) 1545, 1841
BveI ACCTGC 2 cut(s) 14, 545
Cac8I GCNNGC 2 cut(s) 55, 708
CfoI GCGC 2 cut(s) 185, 487
Cfr13I GGNCC 6 cut(s) 116, 566, 682, 908, 1100, 1744
ClaI ATCGAT 2 cut(s) 126, 1593
CseI GACGC 3 cut(s) 946, 1154, 1635
Csp6I GTAC 1 cut(s) 190
CviAII CATG 9 cut(s) 70, 95, 234, 413, 686, 878, 1264, 1404, 1600
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 5 cut(s) 186, 246, 1360, 1493, 1738
DpnI GATC 5 cut(s) 129, 219, 973, 999, 1205
DpnII GATC 5 cut(s) 127, 217, 971, 997, 1203
DraI TTTAAA 2 cut(s) 979, 1428
DrdI GACNNNNNNGTC 1 cut(s) 1054
DseDI GACNNNNNNGTC 1 cut(s) 1054
EaeI YGGCCR 2 cut(s) 178, 474
EagI CGGCCG 1 cut(s) 178
Eam1104I CTCTTC 3 cut(s) 489, 1250, 1359
EarI CTCTTC 3 cut(s) 489, 1250, 1359
EciI GGCGGA 1 cut(s) 68
EclXI CGGCCG 1 cut(s) 178
Eco130I CCWWGG 2 cut(s) 836, 1809
Eco31I GGTCTC 1 cut(s) 1402
Eco32I GATATC 1 cut(s) 1818
Eco47I GGWCC 2 cut(s) 566, 1744
Eco52I CGGCCG 1 cut(s) 178
Eco57I CTGAAG 3 cut(s) 1013, 1502, 1773
Eco88I CYCGRG 3 cut(s) 1291, 1328, 1436
EcoO109I RGGNCCY 1 cut(s) 1744
EcoRI GAATTC 1 cut(s) 1636
EcoRII CCWGG 1 cut(s) 608
EcoRV GATATC 1 cut(s) 1818
EcoT14I CCWWGG 2 cut(s) 836, 1809
ErhI CCWWGG 2 cut(s) 836, 1809
FaeI CATG 9 cut(s) 73, 98, 237, 416, 689, 881, 1267, 1407, 1603
FalI AAGNNNNNCTT 2 cut(s) 1690, 1722
FaqI GGGAC 1 cut(s) 1453
FatI CATG 9 cut(s) 69, 94, 233, 412, 685, 877, 1263, 1403, 1599
FauNDI CATATG 1 cut(s) 261
FokI GGATG 3 cut(s) 62, 443, 931
FspBI CTAG 6 cut(s) 381, 390, 552, 713, 812, 1840
GlaI GCGC 2 cut(s) 184, 486
HaeIII GGCC 7 cut(s) 39, 117, 180, 476, 683, 909, 1102
HapII CCGG 3 cut(s) 33, 570, 1680
HgaI GACGC 3 cut(s) 946, 1154, 1635
HhaI GCGC 2 cut(s) 185, 487
Hin1I GRCGYC 1 cut(s) 938
Hin1II CATG 9 cut(s) 73, 98, 237, 416, 689, 881, 1267, 1407, 1603
Hin6I GCGC 2 cut(s) 183, 485
HinP1I GCGC 2 cut(s) 183, 485
HincII GTYRAC 1 cut(s) 936
HindII GTYRAC 1 cut(s) 936
HindIII AAGCTT 4 cut(s) 899, 1110, 1334, 1611
HinfI GANTC 8 cut(s) 143, 153, 434, 925, 1055, 1223, 1258, 1734
HpaII CCGG 3 cut(s) 33, 570, 1680
HphI GGTGA 3 cut(s) 22, 584, 1711
Hpy166II GTNNAC 3 cut(s) 891, 936, 1625
Hpy188I TCNGA 6 cut(s) 101, 142, 566, 1363, 1521, 1741
Hpy188III TCNNGA 8 cut(s) 131, 812, 945, 1031, 1330, 1438, 1607, 1731
Hpy8I GTNNAC 3 cut(s) 891, 936, 1625
HpyCH4III ACNGT 6 cut(s) 518, 730, 933, 1155, 1246, 1831
HpyCH4IV ACGT 3 cut(s) 87, 665, 678
HpyCH4V TGCA 9 cut(s) 9, 213, 701, 734, 746, 875, 966, 1388, 1455
HpyF3I CTNAG 5 cut(s) 186, 246, 1360, 1493, 1738
HpySE526I ACGT 3 cut(s) 87, 665, 678
Hsp92I GRCGYC 1 cut(s) 938
Hsp92II CATG 9 cut(s) 73, 98, 237, 416, 689, 881, 1267, 1407, 1603
HspAI GCGC 2 cut(s) 183, 485
Kzo9I GATC 5 cut(s) 127, 217, 971, 997, 1203
LmnI GCTCC 2 cut(s) 303, 1505
Lsp1109I GCAGC 5 cut(s) 197, 527, 567, 730, 1372
LweI GCATC 5 cut(s) 154, 331, 855, 1280, 1397
MaeI CTAG 6 cut(s) 381, 390, 552, 713, 812, 1840
MaeII ACGT 3 cut(s) 87, 665, 678
MaeIII GTNAC 4 cut(s) 28, 579, 1007, 1371
MalI GATC 5 cut(s) 129, 219, 973, 999, 1205
MbiI CCGCTC 1 cut(s) 53
MboI GATC 5 cut(s) 127, 217, 971, 997, 1203
MfeI CAATTG 2 cut(s) 870, 1050
MflI RGATCY 1 cut(s) 971
MlyI GAGTC 5 cut(s) 152, 162, 443, 1064, 1267
MmeI TCCRAC 1 cut(s) 171
MroXI GAANNNNTTC 1 cut(s) 89
MseI TTAA 8 cut(s) 351, 363, 522, 783, 978, 1041, 1427, 1467
MslI CAYNNNNRTG 2 cut(s) 696, 1153
MspA1I CMGCKG 2 cut(s) 210, 857
MspCI CTTAAG 2 cut(s) 1040, 1466
MspI CCGG 3 cut(s) 33, 570, 1680
MspR9I CCNGG 2 cut(s) 570, 610
MunI CAATTG 2 cut(s) 870, 1050
MvaI CCWGG 1 cut(s) 610
MvnI CGCG 1 cut(s) 183
NciI CCSGG 1 cut(s) 570
NdeI CATATG 1 cut(s) 261
NdeII GATC 5 cut(s) 127, 217, 971, 997, 1203
NlaIII CATG 9 cut(s) 73, 98, 237, 416, 689, 881, 1267, 1407, 1603
NlaIV GGNNCC 6 cut(s) 118, 270, 568, 588, 777, 1746
NmeAIII GCCGAG 2 cut(s) 65, 93
NmuCI GTSAC 2 cut(s) 28, 1371
NspI RCATGY 2 cut(s) 237, 881
NspV TTCGAA 1 cut(s) 1253
PaeR7I CTCGAG 1 cut(s) 1328
PciI ACATGT 1 cut(s) 877
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PdmI GAANNNNTTC 1 cut(s) 89
PfeI GAWTC 3 cut(s) 925, 1223, 1734
PleI GAGTC 5 cut(s) 151, 161, 442, 1063, 1266
PpsI GAGTC 5 cut(s) 151, 161, 442, 1063, 1266
Ppu21I YACGTR 1 cut(s) 666
PpuMI RGGWCCY 1 cut(s) 1744
PscI ACATGT 1 cut(s) 877
PsiI TTATAA 1 cut(s) 764
Psp5II RGGWCCY 1 cut(s) 1744
Psp6I CCWGG 1 cut(s) 608
PspGI CCWGG 1 cut(s) 608
PspN4I GGNNCC 6 cut(s) 118, 270, 568, 588, 777, 1746
PspPI GGNCC 6 cut(s) 116, 566, 682, 908, 1100, 1744
PspPPI RGGWCCY 1 cut(s) 1744
PstI CTGCAG 1 cut(s) 748
PsuI RGATCY 1 cut(s) 971
PvuII CAGCTG 1 cut(s) 857
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
RseI CAYNNNNRTG 2 cut(s) 696, 1153
SaqAI TTAA 8 cut(s) 351, 363, 522, 783, 978, 1041, 1427, 1467
Sau3AI GATC 5 cut(s) 127, 217, 971, 997, 1203
Sau96I GGNCC 6 cut(s) 116, 566, 682, 908, 1100, 1744
SchI GAGTC 5 cut(s) 152, 162, 443, 1064, 1267
ScrFI CCNGG 2 cut(s) 570, 610
SfaNI GCATC 5 cut(s) 154, 331, 855, 1280, 1397
SfcI CTRYAG 2 cut(s) 744, 852
Sfr274I CTCGAG 1 cut(s) 1328
SfuI TTCGAA 1 cut(s) 1253
SinI GGWCC 2 cut(s) 566, 1744
SlaI CTCGAG 1 cut(s) 1328
SmiMI CAYNNNNRTG 2 cut(s) 696, 1153
SmlI CTYRAG 5 cut(s) 1040, 1069, 1328, 1466, 1729
SmoI CTYRAG 5 cut(s) 1040, 1069, 1328, 1466, 1729
SsiI CCGC 9 cut(s) 51, 79, 181, 208, 266, 606, 1131, 1472, 1770
SspMI CTAG 6 cut(s) 381, 390, 552, 713, 812, 1840
StyD4I CCNGG 2 cut(s) 568, 608
StyI CCWWGG 2 cut(s) 836, 1809
TaaI ACNGT 6 cut(s) 518, 730, 933, 1155, 1246, 1831
TaiI ACGT 3 cut(s) 90, 668, 681
TaqI TCGA 8 cut(s) 126, 132, 201, 1000, 1084, 1253, 1329, 1593
TaqII GACCGA 1 cut(s) 298
TatI WGTACW 1 cut(s) 189
TauI GCSGC 5 cut(s) 53, 183, 269, 608, 1475
TfiI GAWTC 3 cut(s) 925, 1223, 1734
Tru1I TTAA 8 cut(s) 351, 363, 522, 783, 978, 1041, 1427, 1467
Tru9I TTAA 8 cut(s) 351, 363, 522, 783, 978, 1041, 1427, 1467
TscAI CASTG 2 cut(s) 1552, 1841
TseFI GTSAC 2 cut(s) 28, 1371
TseI GCWGC 5 cut(s) 210, 540, 555, 743, 1385
Tsp45I GTSAC 2 cut(s) 28, 1371
TspDTI ATGAA 7 cut(s) 401, 619, 843, 935, 1207, 1276, 1587
TspGWI ACGGA 2 cut(s) 165, 579
TspRI CASTG 2 cut(s) 1552, 1841
Vha464I CTTAAG 2 cut(s) 1040, 1466
VpaK11BI GGWCC 2 cut(s) 566, 1744
XapI RAATTY 1 cut(s) 1636
XbaI TCTAGA 1 cut(s) 811
XceI RCATGY 2 cut(s) 237, 881
XhoI CTCGAG 1 cut(s) 1328
XmnI GAANNNNTTC 1 cut(s) 89
XspI CTAG 6 cut(s) 381, 390, 552, 713, 812, 1840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.